Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001935

Physical Location & Seq

Genomic Coordinates Reverse (-)

13866287 .. 13868195

Gene Structure 1909 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00293660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGAAGTCGTACCAGTTCATTTTTTTCATAATATGTTTATGTCATGTATCCATAAATCCAAATGCCAGTAATGTTAGCTCCAACTTGCTCAAAGTTCCTTTGCCTGTATTCAAACAGTCATTCCAGAAAACCATTACAGTTGTGCAAAATGTGAGCTCTACCTTGTCCACTTTGCATGTTGATCACGATCAGAATTCTAATAATGTCATTTCATACTGTGAGGATTTGCTTGATTCAACAGGTGAAGCCTTGAATTGGTCCCTTTCCACAACTCATGAGCTAAAAGGTGATGTCCTATCCCACACAAGAACTTGGTTGAAAACTTCAGTCACAAGGCATAAGACATGCATACGAGCTTTCGATCGCACAAACAATTCCATTGTTGCATTAGGCTTCAAACTAGTGACCTCATTAATCCTTGAGGTCCTTGGCATGTTGCCTGAGCTGCCTAATGCTCTTGATCTAGAACCTAGATCACATCAGACTCAGTTCCAGCAAATCAGTTATTGGAAGAATAACCCTAACGTCATCGTGTCTAAGGATAGGACTGGAAATTTCCAAAAGATAATGCAGGCAGTAGAGGCTGTACCGGATCATAGCCGGGAGCGTTTTGTGATCTTGGTGAAGAGAGGAGTTTACCAGGAAAATGTGGATATCAATGTGAAAAAGACTAATTTGGTTTTGATTGGAGAGGGCATGGACGTAACTACCATAACTGGACATAAGAGTAATGGTACTGGTTGGCGTACATTTACCTCGGCAACATTTACTGTGAAGGCAGAAGGCTTCATTGCAATCGATATAGGGTTTGAGAACACAGCCGGACCACACAACGGCCAGGCAGTGGCACTTCTAGTCAAGGGTGACCAATCAGCTTTCTTCCGATGCAAGATAAGTGGATACCAAGACACATTGTATGCACACAACAGCCGCCAGTTCTTTAGGGAATGCAAGATCAGTGGCACTGTCGACTTCATCTTCGGCTACAGCACTGCAGTTTTCCAAATGTGCACCATTATTGCCAAGAAAAGACCTAATCATGGCCTGGTTACAGTTACTGCCCACGGTCGTTTCGAAGAAGACTCAACCGGGTTTACATTCCAATTTTGTGACGTGACTGCAGATGCTGATTTGTTGAGCAACATTACAGCTACACCCACATACTTGGGTCGACCTTGGGGTAATTACTCACGTACAGTTTTCATGCAATCGACCCTAGGTAGCATAGTAACTCCCAAGGGTTGGATTGAATGGAATGGAAACCGTGGCATTGACACCCTGTATTATGGTGAGTTCATGAACAAAGGGCCAGGTTCCAAGCTCGCAGGGCGGGTCAGATGGCTTGGTCATCATACTGATCCATCTGAGGCCGATCAGTTTACGGCCACCAACTTTATTGACGGAGATTCTTGGCTGCCATCCACAGGTATCCCATACACATCAAAATTGGGGAATCCAATACCTCAACCTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

491

Amino Acids

54.68

Weight (kDa)

8.31

Isoelectric Point (pI)

22.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	13 - 124	4.1e-10	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	175 - 469	1.2e-122	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001935)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17310 FvH4_6g17430
malus_domestica	MD01G1105400.v1.1 MD03G1086400.v1.1 MD04G1225800.v1.1 MD06G1080700.v1.1
prunus_persica	Prupe.2G210700_v2.0.a1 Prupe.2G216300_v2.0.a1 Prupe.6G344600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0362901 RchiOBHm_Chr1g0362911 RchiOBHm_Chr3g0450671 RchiOBHm_Chr3g0471441
rosa_laevigata	RLG00000024178 RLG00000025712
rosa_multiflora	Rmu_co8224930.1_g000001 Rmu_co8324923.1_g000001 Rmu_sc0000078.1_g000035 Rmu_sc0001502.1_g000007 Rmu_sc0001502.1_g000008 Rmu_sc0006582.1_g000010
rosa_roxburghii	Rroxscaffold_4G00293660 Rroxscaffold_6G00409950 Rroxscaffold_6G00428330
rosa_rugosa	Rorug01G0302100 Rorug01G0302100 Rorug02G0629500 Rorug03G0117800
rosa_samantha	Rh1AG311400 Rh1AG311500 Rh1BG274600 Rh1CG291800 Rh1DG305300 Rh3AG030300 Rh3AG167600 Rh3BG030600 Rh3BG030700 Rh3BG193100 Rh3CG029500 Rh3CG183500 Rh3DG030200 Rh3DG030300 Rh3DG182400
rosa_wichuraiana	Rw1G027600 Rw3G002330 Rw3G015790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	844, 1242
AccI	GTMKAC	2 cut(s)	969, 1171
AciI	CCGC	2 cut(s)	931, 1330
AclWI	GGATC	2 cut(s)	600, 1352
AcoI	YGGCCR	2 cut(s)	835, 1383
AcsI	RAATTY	2 cut(s)	193, 553
AcuI	CTGAAG	1 cut(s)	309
AfaI	GTAC	5 cut(s)	11, 588, 736, 748, 1195
AfiI	CCNNNNNNNGG	6 cut(s)	255, 833, 844, 1040, 1242, 1424
AgsI	TTSAA	6 cut(s)	112, 237, 253, 319, 397, 1250
AhlI	ACTAGT	1 cut(s)	400
AjiI	CACGTC	1 cut(s)	1114
AjnI	CCWGG	4 cut(s)	639, 837, 1044, 1309
AluBI	AGCT	9 cut(s)	78, 156, 280, 356, 445, 875, 1151, 1321, 1473
AluI	AGCT	9 cut(s)	78, 156, 280, 356, 445, 875, 1151, 1321, 1473
Alw21I	GWGCWC	2 cut(s)	158, 1013
Alw44I	GTGCAC	1 cut(s)	1009
AlwI	GGATC	2 cut(s)	600, 1352
AlwNI	CAGNNNCTG	2 cut(s)	1058, 1127
AoxI	GGCC	5 cut(s)	835, 1042, 1307, 1368, 1383
ApaLI	GTGCAC	1 cut(s)	1009
ApeKI	GCWGC	2 cut(s)	445, 1414
ApoI	RAATTY	2 cut(s)	193, 553
ArsI	GACNNNNNNTTYG	2 cut(s)	962, 994
AseI	ATTAAT	1 cut(s)	413
AspA2I	CCTAGG	1 cut(s)	1216
AspS9I	GGNCC	4 cut(s)	258, 424, 824, 1307
AsuC2I	CCSGG	2 cut(s)	602, 1090
AsuHPI	GGTGA	5 cut(s)	254, 299, 634, 875, 1301
AsuII	TTCGAA	1 cut(s)	1074
AvaII	GGWCC	3 cut(s)	258, 424, 824
AvrII	CCTAGG	1 cut(s)	1216
BaeGI	GKGCMC	1 cut(s)	1013
BanII	GRGCYC	1 cut(s)	158
BbsI	GAAGAC	1 cut(s)	1086
Bbv12I	GWGCWC	2 cut(s)	158, 1013
BbvI	GCAGC	2 cut(s)	432, 1401
BccI	CCATC	3 cut(s)	1332, 1369, 1426
BceAI	ACGGC	2 cut(s)	850, 1398
BciT130I	CCWGG	4 cut(s)	641, 839, 1046, 1311
BciVI	GTATCC	3 cut(s)	58, 893, 1439
BclI	TGATCA	1 cut(s)	181
BcnI	CCSGG	2 cut(s)	602, 1090
BcuI	ACTAGT	1 cut(s)	400
BfaI	CTAG	6 cut(s)	401, 464, 471, 854, 1217, 1470
BfmI	CTRYAG	3 cut(s)	985, 993, 1119
BfuI	GTATCC	3 cut(s)	58, 893, 1439
BisI	GCNGC	3 cut(s)	446, 931, 1415
BlnI	CCTAGG	1 cut(s)	1216
BlsI	GCNGC	3 cut(s)	447, 932, 1416
Bme1390I	CCNGG	6 cut(s)	602, 641, 839, 1046, 1090, 1311
Bme18I	GGWCC	3 cut(s)	258, 424, 824
BmgBI	CACGTC	1 cut(s)	1114
BmgT120I	GGNCC	4 cut(s)	258, 424, 824, 1307
BmiI	GGNNCC	2 cut(s)	260, 1315
BmrFI	CCNGG	6 cut(s)	602, 641, 839, 1046, 1090, 1311
BmsI	GCATC	2 cut(s)	875, 1114
BpiI	GAAGAC	1 cut(s)	1086
Bpu10I	CCTNAGC	1 cut(s)	441
Bpu14I	TTCGAA	1 cut(s)	1074
BpuEI	CTTGAG	1 cut(s)	440
BpuMI	CCSGG	2 cut(s)	602, 1090
Bsa29I	ATCGAT	1 cut(s)	798
BsaAI	YACGTR	1 cut(s)	1193
BsaBI	GATNNNNATC	1 cut(s)	186
BsaJI	CCNNGG	7 cut(s)	427, 756, 1063, 1175, 1216, 1236, 1264
BsaWI	WCCGGW	1 cut(s)	589
Bsc4I	CCNNNNNNNGG	6 cut(s)	255, 833, 844, 1040, 1242, 1424
Bse1I	ACTGG	6 cut(s)	13, 66, 553, 721, 742, 934
Bse3DI	GCAATG	1 cut(s)	789
Bse8I	GATNNNNATC	1 cut(s)	186
BseBI	CCWGG	4 cut(s)	641, 839, 1046, 1311
BseCI	ATCGAT	1 cut(s)	798
BseDI	CCNNGG	7 cut(s)	427, 756, 1063, 1175, 1216, 1236, 1264
BseGI	GGATG	1 cut(s)	1418
BseJI	GATNNNNATC	1 cut(s)	186
BseLI	CCNNNNNNNGG	6 cut(s)	255, 833, 844, 1040, 1242, 1424
BseMI	GCAATG	1 cut(s)	789
BseMII	CTCAG	3 cut(s)	432, 500, 1356
BseNI	ACTGG	6 cut(s)	13, 66, 553, 721, 742, 934
BseRI	GAGGAG	1 cut(s)	645
BseSI	GKGCMC	1 cut(s)	1013
BseXI	GCAGC	2 cut(s)	432, 1401
Bsh1285I	CGRYCG	2 cut(s)	364, 1069
BshFI	GGCC	5 cut(s)	837, 1044, 1309, 1370, 1385
BshVI	ATCGAT	1 cut(s)	798
BsiEI	CGRYCG	2 cut(s)	364, 1069
BsiHKAI	GWGCWC	2 cut(s)	158, 1013
BsiSI	CCGG	4 cut(s)	590, 601, 822, 1089
BslFI	GGGAC	1 cut(s)	244
BslI	CCNNNNNNNGG	6 cut(s)	255, 833, 844, 1040, 1242, 1424
BsmFI	GGGAC	1 cut(s)	244
BsmI	GAATGC	1 cut(s)	953
BsnI	GGCC	5 cut(s)	837, 1044, 1309, 1370, 1385
Bsp119I	TTCGAA	1 cut(s)	1074
Bsp1286I	GDGCHC	2 cut(s)	158, 1013
BspACI	CCGC	2 cut(s)	931, 1330
BspANI	GGCC	5 cut(s)	837, 1044, 1309, 1370, 1385
BspCNI	CTCAG	3 cut(s)	433, 499, 1357
BspDI	ATCGAT	1 cut(s)	798
BspHI	TCATGA	2 cut(s)	274, 1296
BspLI	GGNNCC	2 cut(s)	260, 1315
BspMAI	CTGCAG	2 cut(s)	997, 1123
BspPI	GGATC	2 cut(s)	600, 1352
BspT104I	TTCGAA	1 cut(s)	1074
BsrDI	GCAATG	1 cut(s)	789
BsrI	ACTGG	6 cut(s)	13, 66, 553, 721, 742, 934
BssECI	CCNNGG	7 cut(s)	427, 756, 1063, 1175, 1216, 1236, 1264
BssT1I	CCWWGG	4 cut(s)	427, 1175, 1216, 1236
Bst2UI	CCWGG	4 cut(s)	641, 839, 1046, 1311
Bst4CI	ACNGT	9 cut(s)	117, 139, 218, 772, 967, 1054, 1067, 1198, 1265
Bst6I	CTCTTC	1 cut(s)	620
BstBAI	YACGTR	1 cut(s)	1193
BstBI	TTCGAA	1 cut(s)	1074
BstC8I	GCNNGC	2 cut(s)	573, 1323
BstDEI	CTNAG	4 cut(s)	441, 486, 537, 1365
BstDSI	CCRYGG	2 cut(s)	1063, 1264
BstEII	GGTNACC	1 cut(s)	863
BstF5I	GGATG	1 cut(s)	1418
BstMCI	CGRYCG	2 cut(s)	364, 1069
BstMWI	GCNNNNNNNGC	3 cut(s)	445, 581, 1327
BstNI	CCWGG	4 cut(s)	641, 839, 1046, 1311
BstNSI	RCATGY	3 cut(s)	179, 348, 436
BstPI	GGTNACC	1 cut(s)	863
BstSCI	CCNGG	6 cut(s)	600, 639, 837, 1044, 1088, 1309
BstSFI	CTRYAG	3 cut(s)	985, 993, 1119
BstSLI	GKGCMC	1 cut(s)	1013
BstV1I	GCAGC	2 cut(s)	432, 1401
BstV2I	GAAGAC	1 cut(s)	1086
BstXI	CCANNNNNNTGG	1 cut(s)	1165
Bsu15I	ATCGAT	1 cut(s)	798
BsuI	GTATCC	3 cut(s)	58, 893, 1439
BsuRI	GGCC	5 cut(s)	837, 1044, 1309, 1370, 1385
BsuTUI	ATCGAT	1 cut(s)	798
BtgI	CCRYGG	2 cut(s)	1063, 1264
BtrI	CACGTC	1 cut(s)	1114
BtsCI	GGATG	1 cut(s)	1418
BtsI	GCAGTG	2 cut(s)	849, 990
BtsIMutI	CAGTG	4 cut(s)	849, 963, 964, 990
Cac8I	GCNNGC	2 cut(s)	573, 1323
CaiI	CAGNNNCTG	2 cut(s)	1058, 1127
CciI	TCATGA	2 cut(s)	274, 1296
Cfr13I	GGNCC	4 cut(s)	258, 424, 824, 1307
ClaI	ATCGAT	1 cut(s)	798
Csp6I	GTAC	5 cut(s)	10, 587, 735, 747, 1194
CspCI	CAANNNNNGTGG	4 cut(s)	877, 912, 940, 975
CviAII	CATG	9 cut(s)	44, 176, 275, 345, 433, 697, 1040, 1204, 1297
CviQI	GTAC	5 cut(s)	10, 587, 735, 747, 1194
DdeI	CTNAG	4 cut(s)	441, 486, 537, 1365
EaeI	YGGCCR	2 cut(s)	835, 1383
Eam1104I	CTCTTC	1 cut(s)	620
EarI	CTCTTC	1 cut(s)	620
Ecl136II	GAGCTC	1 cut(s)	156
Eco130I	CCWWGG	4 cut(s)	427, 1175, 1216, 1236
Eco24I	GRGCYC	1 cut(s)	158
Eco32I	GATATC	1 cut(s)	655
Eco47I	GGWCC	3 cut(s)	258, 424, 824
Eco53kI	GAGCTC	1 cut(s)	156
Eco57I	CTGAAG	1 cut(s)	309
Eco91I	GGTNACC	1 cut(s)	863
EcoICRI	GAGCTC	1 cut(s)	156
EcoO109I	RGGNCCY	1 cut(s)	424
EcoO65I	GGTNACC	1 cut(s)	863
EcoRI	GAATTC	1 cut(s)	193
EcoRII	CCWGG	4 cut(s)	639, 837, 1044, 1309
EcoRV	GATATC	1 cut(s)	655
EcoT14I	CCWWGG	4 cut(s)	427, 1175, 1216, 1236
EcoT22I	ATGCAT	1 cut(s)	350
EcoT38I	GRGCYC	1 cut(s)	158
ErhI	CCWWGG	4 cut(s)	427, 1175, 1216, 1236
FaeI	CATG	9 cut(s)	47, 179, 278, 348, 436, 700, 1043, 1207, 1300
FaqI	GGGAC	1 cut(s)	244
FatI	CATG	9 cut(s)	43, 175, 274, 344, 432, 696, 1039, 1203, 1296
FauI	CCCGC	1 cut(s)	1323
FbaI	TGATCA	1 cut(s)	181
FblI	GTMKAC	2 cut(s)	969, 1171
Fnu4HI	GCNGC	3 cut(s)	446, 931, 1415
FokI	GGATG	1 cut(s)	1405
FriOI	GRGCYC	1 cut(s)	158
Fsp4HI	GCNGC	3 cut(s)	446, 931, 1415
FspBI	CTAG	6 cut(s)	401, 464, 471, 854, 1217, 1470
GluI	GCNGC	3 cut(s)	446, 931, 1415
HaeIII	GGCC	5 cut(s)	837, 1044, 1309, 1370, 1385
HapII	CCGG	4 cut(s)	590, 601, 822, 1089
Hin1II	CATG	9 cut(s)	47, 179, 278, 348, 436, 700, 1043, 1207, 1300
HincII	GTYRAC	2 cut(s)	970, 1172
HindII	GTYRAC	2 cut(s)	970, 1172
HinfI	GANTC	5 cut(s)	233, 484, 1082, 1406, 1453
HpaII	CCGG	4 cut(s)	590, 601, 822, 1089
HphI	GGTGA	5 cut(s)	254, 299, 634, 875, 1301
Hpy166II	GTNNAC	7 cut(s)	168, 637, 970, 1011, 1095, 1172, 1380
Hpy188I	TCNGA	5 cut(s)	192, 483, 884, 1337, 1366
Hpy188III	TCNNGA	6 cut(s)	124, 185, 275, 458, 464, 1297
Hpy8I	GTNNAC	7 cut(s)	168, 637, 970, 1011, 1095, 1172, 1380
HpyAV	CCTTC	2 cut(s)	769, 776
HpyCH4III	ACNGT	9 cut(s)	117, 139, 218, 772, 967, 1054, 1067, 1198, 1265
HpyCH4IV	ACGT	4 cut(s)	525, 702, 1113, 1192
HpyF10VI	GCNNNNNNNGC	3 cut(s)	445, 581, 1327
HpyF3I	CTNAG	4 cut(s)	441, 486, 537, 1365
HpySE526I	ACGT	4 cut(s)	525, 702, 1113, 1192
Hsp92II	CATG	9 cut(s)	47, 179, 278, 348, 436, 700, 1043, 1207, 1300
Ksp22I	TGATCA	1 cut(s)	181
LmnI	GCTCC	2 cut(s)	83, 604
Lsp1109I	GCAGC	2 cut(s)	432, 1401
LweI	GCATC	2 cut(s)	875, 1114
MaeI	CTAG	6 cut(s)	401, 464, 471, 854, 1217, 1470
MaeII	ACGT	4 cut(s)	525, 702, 1113, 1192
MaeIII	GTNAC	9 cut(s)	328, 403, 703, 863, 1048, 1054, 1109, 1114, 1228
MboII	GAAGA	6 cut(s)	523, 637, 871, 970, 1088, 1091
MhlI	GDGCHC	2 cut(s)	158, 1013
MluCI	AATT	8 cut(s)	193, 253, 373, 553, 673, 1103, 1183, 1445
MlyI	GAGTC	2 cut(s)	478, 1076
MmeI	TCCRAC	2 cut(s)	105, 1223
MnlI	CCTC	9 cut(s)	214, 415, 418, 574, 623, 685, 766, 1360, 1473
Mph1103I	ATGCAT	1 cut(s)	350
MseI	TTAA	1 cut(s)	413
MspI	CCGG	4 cut(s)	590, 601, 822, 1089
MspR9I	CCNGG	6 cut(s)	602, 641, 839, 1046, 1090, 1311
Mva1269I	GAATGC	1 cut(s)	953
MvaI	CCWGG	4 cut(s)	641, 839, 1046, 1311
MwoI	GCNNNNNNNGC	3 cut(s)	445, 581, 1327
NciI	CCSGG	2 cut(s)	602, 1090
NlaIII	CATG	9 cut(s)	47, 179, 278, 348, 436, 700, 1043, 1207, 1300
NlaIV	GGNNCC	2 cut(s)	260, 1315
NmeAIII	GCCGAG	1 cut(s)	737
NmuCI	GTSAC	5 cut(s)	328, 403, 863, 1109, 1114
NsiI	ATGCAT	1 cut(s)	350
NspI	RCATGY	3 cut(s)	179, 348, 436
NspV	TTCGAA	1 cut(s)	1074
PagI	TCATGA	2 cut(s)	274, 1296
PcsI	WCGNNNNNNNCGW	1 cut(s)	1071
PctI	GAATGC	1 cut(s)	953
PfeI	GAWTC	3 cut(s)	233, 1406, 1453
PflMI	CCANNNNNTGG	2 cut(s)	844, 1242
PkrI	GCNGC	3 cut(s)	447, 932, 1416
Ple19I	CGATCG	1 cut(s)	364
PleI	GAGTC	2 cut(s)	478, 1076
PpsI	GAGTC	2 cut(s)	478, 1076
Ppu21I	YACGTR	1 cut(s)	1193
PpuMI	RGGWCCY	1 cut(s)	424
PshBI	ATTAAT	1 cut(s)	413
Psp124BI	GAGCTC	1 cut(s)	158
Psp5II	RGGWCCY	1 cut(s)	424
Psp6I	CCWGG	4 cut(s)	639, 837, 1044, 1309
PspEI	GGTNACC	1 cut(s)	863
PspGI	CCWGG	4 cut(s)	639, 837, 1044, 1309
PspN4I	GGNNCC	2 cut(s)	260, 1315
PspPI	GGNCC	4 cut(s)	258, 424, 824, 1307
PspPPI	RGGWCCY	1 cut(s)	424
PstI	CTGCAG	2 cut(s)	997, 1123
PstNI	CAGNNNCTG	2 cut(s)	1058, 1127
PvuI	CGATCG	1 cut(s)	364
RsaI	GTAC	5 cut(s)	11, 588, 736, 748, 1195
RsaNI	GTAC	5 cut(s)	10, 587, 735, 747, 1194
SacI	GAGCTC	1 cut(s)	158
SalI	GTCGAC	2 cut(s)	968, 1170
SaqAI	TTAA	1 cut(s)	413
SatI	GCNGC	3 cut(s)	446, 931, 1415
Sau96I	GGNCC	4 cut(s)	258, 424, 824, 1307
SchI	GAGTC	2 cut(s)	478, 1076
ScrFI	CCNGG	6 cut(s)	602, 641, 839, 1046, 1090, 1311
SduI	GDGCHC	2 cut(s)	158, 1013
SfaNI	GCATC	2 cut(s)	875, 1114
SfcI	CTRYAG	3 cut(s)	985, 993, 1119
SfuI	TTCGAA	1 cut(s)	1074
SinI	GGWCC	3 cut(s)	258, 424, 824
SmlI	CTYRAG	1 cut(s)	419
SmoI	CTYRAG	1 cut(s)	419
SpeI	ACTAGT	1 cut(s)	400
Sse9I	AATT	8 cut(s)	193, 253, 373, 553, 673, 1103, 1183, 1445
SsiI	CCGC	2 cut(s)	931, 1330
SspMI	CTAG	6 cut(s)	401, 464, 471, 854, 1217, 1470
SstI	GAGCTC	1 cut(s)	158
StyD4I	CCNGG	6 cut(s)	600, 639, 837, 1044, 1088, 1309
StyI	CCWWGG	4 cut(s)	427, 1175, 1216, 1236
TaaI	ACNGT	9 cut(s)	117, 139, 218, 772, 967, 1054, 1067, 1198, 1265
TaiI	ACGT	4 cut(s)	528, 705, 1116, 1195
TaqI	TCGA	6 cut(s)	360, 798, 969, 1074, 1171, 1211
TasI	AATT	8 cut(s)	193, 253, 373, 553, 673, 1103, 1183, 1445
TauI	GCSGC	1 cut(s)	933
TfiI	GAWTC	3 cut(s)	233, 1406, 1453
Tru1I	TTAA	1 cut(s)	413
Tru9I	TTAA	1 cut(s)	413
TscAI	CASTG	4 cut(s)	849, 964, 970, 997
TseFI	GTSAC	5 cut(s)	328, 403, 863, 1109, 1114
TseI	GCWGC	2 cut(s)	445, 1414
Tsp45I	GTSAC	5 cut(s)	328, 403, 863, 1109, 1114
TspDTI	ATGAA	9 cut(s)	7, 16, 17, 201, 778, 964, 1192, 1285, 1313
TspGWI	ACGGA	1 cut(s)	1416
TspRI	CASTG	4 cut(s)	849, 964, 970, 997
Van91I	CCANNNNNTGG	2 cut(s)	844, 1242
VneI	GTGCAC	1 cut(s)	1009
VpaK11BI	GGWCC	3 cut(s)	258, 424, 824
VspI	ATTAAT	1 cut(s)	413
XapI	RAATTY	2 cut(s)	193, 553
XbaI	TCTAGA	1 cut(s)	463
XceI	RCATGY	3 cut(s)	179, 348, 436
XmaJI	CCTAGG	1 cut(s)	1216
XmiI	GTMKAC	2 cut(s)	969, 1171
XspI	CTAG	6 cut(s)	401, 464, 471, 854, 1217, 1470
Zsp2I	ATGCAT	1 cut(s)	350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.