RchiOBHm_Chr3g0450671

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001935
Physical Location & Seq
Reverse (-)
2113687 .. 2115774
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41799

Sequence Viewer

Length: 1548 bp
ATGAAGCTCTCCGATAGTTTCTATCTTTTCTTCACTTGCTTCATTGCTCTATCCATTAACTCACATGCTAGGTCTAGTTCTAATATCGGCTCATCCCGCTTCGTAAAAGTCCCCTTGCCTTTGTTCAGAGAGTCACTGGTGTCTACTATGGAACTTGTTCAAAGTGTGGCCTCTACCATGTCCCTAATCCATGGTGACATTGATGATAACGATGATGATGGTCCAAATCTCAATGTTTCTCTTCCACATTGTGCATATTTGCTTGGTGAAACTGCTGGTGTTATGAATTGGACCCTTTCGGCGATTAATCAGCAATATACTCAAGGTCATAATAAACAATTTTATACAACAGGTGATGCCATTTCCCATGCGAGGACACTGTTGAGTGGTAGTCTTGGCAGCCAAGAAATGTGTACGCAAACGCTTGATGGTACCAAACATTTCATGGTTTCAAGGAGGCTCAAACAAGTGACCACATCCATATATGATATCCTAGATATGTTGCAAGTGCCACGATTTGGTCCTGATTTTTCCAGGGCCACTGGTGCTTCTTTACTTGGTTCGACTAGATTCGACGGCGTTAAGATTTCACAAAAACCAAACGTCATTGTTTCACAAGACGGGAAGGGAGATTTCAAGCTAATAATGGACGCGATTAAGGAAGCACCAAGTCACTCCACAAATTACTTTGTGATACTTGTTAAGAGAGGAGTTTACCATGAATACGTGAACATTGATGAGACAAAATCGAATCTGGTCTTGATGGGAGAGGGCATGGATGTTACTACCATTTCCGGAAGTAAAAGCGTAAGGGGTGGTCAGCCAACATTTGATACAGCCACATTTGCCGTTAGGGCCCTAGGATTCATTGCAATGGACATAGGGTTTGAGAACACTGCCGGACAAGAGAACATGCAGGCGTTGGCGGTTCTCTCCAGCGGCGACCGCTCCGCCTTTTACAGATGCAAGATGACCGGAAATCAAGACACATTGCTAGTGCAAGCTGGCCGCCAATTCTTCCGAGAATGCAACATCACGGGCACCGTTGACTTCATCTTTGGCTATGGCAGTGCTGTCTTTCAAAAATGCGACATTTACATCAAGAAAAATCCAATAGGCGGAACCAGCGTCATCGCGGCCCATGGCCGTAACAACCCATCAGACCCATCAGGCTTTTCATTCCACCTCTGCACCATCACCGGAGACCCCAATGTGGTCATTAAAGACTCGACTAACAACGGAGCGTACCTCGGTCGGAATTGGGGGGTTTATGCACGAACAGTGTTCATAGATTCGACCATAAGCAATGTTCTACGGCCGGAAGGTTGGCTCCAGTGGCAAGGCAAGGGTGTGGACAAAGTGTATTTCGGGGAGTACATGAACAATGGCCCGGGTGCAAACGCTGCAAACCGGGTGAGCTGGCCTGGTTGTCATGCGAGGATGAGCCGATCTGAGGCTGATAGATTTACAGTGGCAAAGCTTATTGATGGAACTTCATGGCTGTCAACTGTAGGTATCCCAAACACAATGGGATTGGATGGATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

515

Amino Acids

56.18

Weight (kDa)

6.83

Isoelectric Point (pI)

31.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 201 - 497 8.2e-110 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 2 cut(s) 431, 1040
AccB7I CCANNNNNTGG 1 cut(s) 518
AccBSI CCGCTC 1 cut(s) 948
AccI GTMKAC 1 cut(s) 143
AccII CGCG 2 cut(s) 653, 1136
AccIII TCCGGA 1 cut(s) 794
AciI CCGC 8 cut(s) 97, 926, 939, 946, 951, 1009, 1119, 1136
AclWI GGATC 1 cut(s) 1536
AcoI YGGCCR 3 cut(s) 1006, 1144, 1316
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 4 cut(s) 415, 433, 1247, 1376
AfiI CCNNNNNNNGG 5 cut(s) 372, 518, 1118, 1213, 1453
AgsI TTSAA 4 cut(s) 161, 453, 637, 1082
AjnI CCWGG 2 cut(s) 533, 1423
AluBI AGCT 5 cut(s) 7, 640, 1004, 1419, 1480
AluI AGCT 5 cut(s) 7, 640, 1004, 1419, 1480
Alw26I GTCTC 2 cut(s) 734, 1197
AlwI GGATC 1 cut(s) 1536
Ama87I CYCGRG 1 cut(s) 1390
Aor13HI TCCGGA 1 cut(s) 794
AoxI GGCC 9 cut(s) 168, 537, 855, 1006, 1137, 1144, 1316, 1387, 1421
ApaI GGGCCC 1 cut(s) 859
ApeKI GCWGC 2 cut(s) 399, 1403
ArsI GACNNNNNNTTYG 4 cut(s) 869, 901, 1040, 1072
AseI ATTAAT 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 156
Asp718I GGTACC 1 cut(s) 431
AspA2I CCTAGG 1 cut(s) 859
AspS9I GGNCC 8 cut(s) 221, 291, 521, 537, 855, 856, 1138, 1388
AsuC2I CCSGG 3 cut(s) 1391, 1392, 1412
AsuHPI GGTGA 5 cut(s) 206, 278, 365, 1189, 1426
AvaI CYCGRG 1 cut(s) 1390
AvaII GGWCC 3 cut(s) 221, 291, 521
AvrII CCTAGG 1 cut(s) 859
BaeGI GKGCMC 2 cut(s) 859, 1043
BamHI GGATCC 1 cut(s) 1541
BanI GGYRCC 2 cut(s) 431, 1040
BanII GRGCYC 1 cut(s) 859
BbvI GCAGC 2 cut(s) 411, 1390
BccI CCATC 8 cut(s) 212, 422, 757, 1165, 1174, 1202, 1481, 1532
BceAI ACGGC 4 cut(s) 592, 833, 1131, 1331
BciT130I CCWGG 2 cut(s) 535, 1425
BciVI GTATCC 1 cut(s) 1526
BcnI CCSGG 3 cut(s) 1391, 1392, 1412
BcoDI GTCTC 2 cut(s) 734, 1197
BfaI CTAG 6 cut(s) 69, 75, 494, 567, 860, 995
BfmI CTRYAG 1 cut(s) 1509
BfuI GTATCC 1 cut(s) 1526
BglI GCCNNNNNGGC 1 cut(s) 854
BisI GCNGC 5 cut(s) 400, 940, 1009, 1137, 1404
BlnI CCTAGG 1 cut(s) 859
BlsI GCNGC 5 cut(s) 401, 941, 1010, 1138, 1405
Bme1390I CCNGG 5 cut(s) 535, 1391, 1392, 1412, 1425
Bme18I GGWCC 3 cut(s) 221, 291, 521
BmeT110I CYCGRG 1 cut(s) 1390
BmgT120I GGNCC 8 cut(s) 221, 291, 521, 537, 855, 856, 1138, 1388
BmiI GGNNCC 7 cut(s) 293, 433, 857, 1042, 1123, 1331, 1543
BmrFI CCNGG 5 cut(s) 535, 1391, 1392, 1412, 1425
BmsI GCATC 2 cut(s) 346, 953
BplI GAGNNNNNCTC 2 cut(s) 1233, 1265
BpmI CTGGAG 2 cut(s) 919, 1316
BpuEI CTTGAG 1 cut(s) 306
BpuMI CCSGG 3 cut(s) 1391, 1392, 1412
BsaAI YACGTR 1 cut(s) 727
BsaI GGTCTC 1 cut(s) 1197
BsaJI CCNNGG 6 cut(s) 190, 534, 859, 1141, 1249, 1390
BsaWI WCCGGW 3 cut(s) 794, 974, 1199
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 518, 1118, 1213, 1453
Bse1I ACTGG 3 cut(s) 141, 547, 1333
Bse3DI GCAATG 5 cut(s) 42, 867, 879, 989, 1312
BseAI TCCGGA 1 cut(s) 794
BseBI CCWGG 2 cut(s) 535, 1425
BseDI CCNNGG 6 cut(s) 190, 534, 859, 1141, 1249, 1390
BseGI GGATG 5 cut(s) 92, 476, 784, 1446, 1543
BseLI CCNNNNNNNGG 5 cut(s) 372, 518, 1118, 1213, 1453
BseMI GCAATG 5 cut(s) 42, 867, 879, 989, 1312
BseMII CTCAG 1 cut(s) 1443
BseNI ACTGG 3 cut(s) 141, 547, 1333
BseRI GAGGAG 1 cut(s) 723
BseSI GKGCMC 2 cut(s) 859, 1043
BseX3I CGGCCG 1 cut(s) 1316
BseXI GCAGC 2 cut(s) 411, 1390
BsgI GTGCAG 1 cut(s) 1174
Bsh1236I CGCG 2 cut(s) 653, 1136
Bsh1285I CGRYCG 3 cut(s) 946, 1255, 1319
BshFI GGCC 9 cut(s) 170, 539, 857, 1008, 1139, 1146, 1318, 1389, 1423
BshNI GGYRCC 2 cut(s) 431, 1040
BsiEI CGRYCG 3 cut(s) 946, 1255, 1319
BsiHKCI CYCGRG 1 cut(s) 1390
BsiSI CCGG 7 cut(s) 795, 900, 975, 1200, 1319, 1391, 1411
BslFI GGGAC 2 cut(s) 95, 166
BslI CCNNNNNNNGG 5 cut(s) 372, 518, 1118, 1213, 1453
BsmAI GTCTC 2 cut(s) 734, 1197
BsmFI GGGAC 2 cut(s) 95, 166
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 9 cut(s) 170, 539, 857, 1008, 1139, 1146, 1318, 1389, 1423
Bso31I GGTCTC 1 cut(s) 1197
BsoBI CYCGRG 1 cut(s) 1390
Bsp120I GGGCCC 1 cut(s) 855
Bsp1286I GDGCHC 2 cut(s) 859, 1043
Bsp13I TCCGGA 1 cut(s) 794
Bsp143I GATC 2 cut(s) 1448, 1541
Bsp19I CCATGG 2 cut(s) 190, 1141
BspACI CCGC 8 cut(s) 97, 926, 939, 946, 951, 1009, 1119, 1136
BspANI GGCC 9 cut(s) 170, 539, 857, 1008, 1139, 1146, 1318, 1389, 1423
BspCNI CTCAG 1 cut(s) 1444
BspEI TCCGGA 1 cut(s) 794
BspFNI CGCG 2 cut(s) 653, 1136
BspLI GGNNCC 7 cut(s) 293, 433, 857, 1042, 1123, 1331, 1543
BspPI GGATC 1 cut(s) 1536
BspT107I GGYRCC 2 cut(s) 431, 1040
BspTNI GGTCTC 1 cut(s) 1197
BsrBI CCGCTC 1 cut(s) 948
BsrDI GCAATG 5 cut(s) 42, 867, 879, 989, 1312
BsrI ACTGG 3 cut(s) 141, 547, 1333
BssECI CCNNGG 6 cut(s) 190, 534, 859, 1141, 1249, 1390
BssMI GATC 2 cut(s) 1448, 1541
BssT1I CCWWGG 3 cut(s) 190, 859, 1141
Bst2UI CCWGG 2 cut(s) 535, 1425
Bst4CI ACNGT 5 cut(s) 381, 1045, 1282, 1471, 1510
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 1403
BstBAI YACGTR 1 cut(s) 727
BstC8I GCNNGC 4 cut(s) 918, 1002, 1006, 1421
BstDEI CTNAG 1 cut(s) 1452
BstDSI CCRYGG 2 cut(s) 190, 1141
BstF5I GGATG 5 cut(s) 92, 476, 784, 1446, 1543
BstFNI CGCG 2 cut(s) 653, 1136
BstKTI GATC 2 cut(s) 1451, 1544
BstMAI GTCTC 2 cut(s) 734, 1197
BstMBI GATC 2 cut(s) 1448, 1541
BstMCI CGRYCG 3 cut(s) 946, 1255, 1319
BstMWI GCNNNNNNNGC 8 cut(s) 96, 545, 845, 854, 945, 1125, 1336, 1403
BstNI CCWGG 2 cut(s) 535, 1425
BstNSI RCATGY 2 cut(s) 68, 916
BstSCI CCNGG 5 cut(s) 533, 1389, 1390, 1410, 1423
BstSFI CTRYAG 1 cut(s) 1509
BstSLI GKGCMC 2 cut(s) 859, 1043
BstUI CGCG 2 cut(s) 653, 1136
BstV1I GCAGC 2 cut(s) 411, 1390
BstX2I RGATCY 1 cut(s) 1541
BstYI RGATCY 1 cut(s) 1541
BstZI CGGCCG 1 cut(s) 1316
BsuI GTATCC 1 cut(s) 1526
BsuRI GGCC 9 cut(s) 170, 539, 857, 1008, 1139, 1146, 1318, 1389, 1423
BtgI CCRYGG 2 cut(s) 190, 1141
BtgZI GCGATG 1 cut(s) 1117
BtsCI GGATG 5 cut(s) 92, 476, 784, 1446, 1543
BtsI GCAGTG 2 cut(s) 894, 1075
BtsIMutI CAGTG 8 cut(s) 134, 377, 540, 894, 1075, 1287, 1340, 1476
Cac8I GCNNGC 4 cut(s) 918, 1002, 1006, 1421
Cfr13I GGNCC 8 cut(s) 221, 291, 521, 537, 855, 856, 1138, 1388
Cfr9I CCCGGG 1 cut(s) 1390
CseI GACGC 2 cut(s) 659, 1117
Csp6I GTAC 4 cut(s) 414, 432, 1246, 1375
CviQI GTAC 4 cut(s) 414, 432, 1246, 1375
DdeI CTNAG 1 cut(s) 1452
DpnI GATC 2 cut(s) 1450, 1543
DpnII GATC 2 cut(s) 1448, 1541
DraIII CACNNNGTG 1 cut(s) 251
EaeI YGGCCR 3 cut(s) 1006, 1144, 1316
EagI CGGCCG 1 cut(s) 1316
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
EciI GGCGGA 2 cut(s) 940, 1134
EclXI CGGCCG 1 cut(s) 1316
Eco130I CCWWGG 3 cut(s) 190, 859, 1141
Eco24I GRGCYC 1 cut(s) 859
Eco31I GGTCTC 1 cut(s) 1197
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 3 cut(s) 221, 291, 521
Eco52I CGGCCG 1 cut(s) 1316
Eco88I CYCGRG 1 cut(s) 1390
EcoO109I RGGNCCY 2 cut(s) 855, 856
EcoRII CCWGG 2 cut(s) 533, 1423
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 3 cut(s) 190, 859, 1141
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 3 cut(s) 190, 859, 1141
FaqI GGGAC 2 cut(s) 95, 166
FauI CCCGC 1 cut(s) 104
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 5 cut(s) 400, 940, 1009, 1137, 1404
FokI GGATG 4 cut(s) 79, 463, 791, 1453
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 5 cut(s) 400, 940, 1009, 1137, 1404
FspBI CTAG 6 cut(s) 69, 75, 494, 567, 860, 995
GluI GCNGC 5 cut(s) 400, 940, 1009, 1137, 1404
GsuI CTGGAG 2 cut(s) 919, 1316
HaeIII GGCC 9 cut(s) 170, 539, 857, 1008, 1139, 1146, 1318, 1389, 1423
HapII CCGG 7 cut(s) 795, 900, 975, 1200, 1319, 1391, 1411
HgaI GACGC 2 cut(s) 659, 1117
HincII GTYRAC 2 cut(s) 1048, 1506
HindII GTYRAC 2 cut(s) 1048, 1506
HindIII AAGCTT 1 cut(s) 1478
HinfI GANTC 6 cut(s) 131, 570, 751, 864, 1226, 1292
HpaII CCGG 7 cut(s) 795, 900, 975, 1200, 1319, 1391, 1411
HphI GGTGA 5 cut(s) 206, 278, 365, 1189, 1426
Hpy166II GTNNAC 7 cut(s) 144, 414, 715, 730, 1048, 1354, 1506
Hpy188I TCNGA 6 cut(s) 13, 128, 1022, 1162, 1257, 1453
Hpy188III TCNNGA 5 cut(s) 524, 760, 795, 983, 1102
Hpy8I GTNNAC 7 cut(s) 144, 414, 715, 730, 1048, 1354, 1506
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 2 cut(s) 619, 1316
HpyCH4III ACNGT 5 cut(s) 381, 1045, 1282, 1471, 1510
HpyCH4IV ACGT 2 cut(s) 603, 726
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 545, 845, 854, 945, 1125, 1336, 1403
HpyF3I CTNAG 1 cut(s) 1452
HpySE526I ACGT 2 cut(s) 603, 726
Kpn2I TCCGGA 1 cut(s) 794
KpnI GGTACC 1 cut(s) 435
Kzo9I GATC 2 cut(s) 1448, 1541
LmnI GCTCC 3 cut(s) 953, 1241, 1335
Lsp1109I GCAGC 2 cut(s) 411, 1390
LweI GCATC 2 cut(s) 346, 953
MaeI CTAG 6 cut(s) 69, 75, 494, 567, 860, 995
MaeII ACGT 2 cut(s) 603, 726
MaeIII GTNAC 6 cut(s) 132, 194, 469, 671, 781, 1148
MalI GATC 2 cut(s) 1450, 1543
MbiI CCGCTC 1 cut(s) 948
MboI GATC 2 cut(s) 1448, 1541
MboII GAAGA 3 cut(s) 22, 233, 1009
MflI RGATCY 1 cut(s) 1541
MhlI GDGCHC 2 cut(s) 859, 1043
MluCI AATT 5 cut(s) 286, 338, 682, 1013, 1258
MlyI GAGTC 2 cut(s) 140, 1220
MmeI TCCRAC 1 cut(s) 1235
MnlI CCTC 9 cut(s) 181, 366, 450, 701, 763, 1196, 1259, 1431, 1447
MroI TCCGGA 1 cut(s) 794
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 6 cut(s) 57, 306, 582, 657, 702, 1221
MslI CAYNNNNRTG 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 939
MspI CCGG 7 cut(s) 795, 900, 975, 1200, 1319, 1391, 1411
MspR9I CCNGG 5 cut(s) 535, 1391, 1392, 1412, 1425
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 2 cut(s) 535, 1425
MvnI CGCG 2 cut(s) 653, 1136
MwoI GCNNNNNNNGC 8 cut(s) 96, 545, 845, 854, 945, 1125, 1336, 1403
NciI CCSGG 3 cut(s) 1391, 1392, 1412
NcoI CCATGG 2 cut(s) 190, 1141
NdeII GATC 2 cut(s) 1448, 1541
NlaIV GGNNCC 7 cut(s) 293, 433, 857, 1042, 1123, 1331, 1543
NmuCI GTSAC 4 cut(s) 132, 194, 469, 671
NspI RCATGY 2 cut(s) 68, 916
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 4 cut(s) 570, 751, 864, 1292
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 5 cut(s) 401, 941, 1010, 1138, 1405
PleI GAGTC 2 cut(s) 139, 1220
PpsI GAGTC 2 cut(s) 139, 1220
Ppu21I YACGTR 1 cut(s) 727
PshBI ATTAAT 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 533, 1423
PspGI CCWGG 2 cut(s) 533, 1423
PspN4I GGNNCC 7 cut(s) 293, 433, 857, 1042, 1123, 1331, 1543
PspOMI GGGCCC 1 cut(s) 855
PspPI GGNCC 8 cut(s) 221, 291, 521, 537, 855, 856, 1138, 1388
PsuI RGATCY 1 cut(s) 1541
RsaI GTAC 4 cut(s) 415, 433, 1247, 1376
RsaNI GTAC 4 cut(s) 414, 432, 1246, 1375
RseI CAYNNNNRTG 1 cut(s) 872
SaqAI TTAA 6 cut(s) 57, 306, 582, 657, 702, 1221
SatI GCNGC 5 cut(s) 400, 940, 1009, 1137, 1404
Sau3AI GATC 2 cut(s) 1448, 1541
Sau96I GGNCC 8 cut(s) 221, 291, 521, 537, 855, 856, 1138, 1388
SchI GAGTC 2 cut(s) 140, 1220
ScrFI CCNGG 5 cut(s) 535, 1391, 1392, 1412, 1425
SduI GDGCHC 2 cut(s) 859, 1043
SfaNI GCATC 2 cut(s) 346, 953
SfcI CTRYAG 1 cut(s) 1509
SinI GGWCC 3 cut(s) 221, 291, 521
SmaI CCCGGG 1 cut(s) 1392
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 5 cut(s) 286, 338, 682, 1013, 1258
SsiI CCGC 8 cut(s) 97, 926, 939, 946, 951, 1009, 1119, 1136
SspMI CTAG 6 cut(s) 69, 75, 494, 567, 860, 995
StyD4I CCNGG 5 cut(s) 533, 1389, 1390, 1410, 1423
StyI CCWWGG 3 cut(s) 190, 859, 1141
TaaI ACNGT 5 cut(s) 381, 1045, 1282, 1471, 1510
TaiI ACGT 2 cut(s) 606, 729
TaqI TCGA 5 cut(s) 563, 573, 749, 1229, 1295
TaqII GACCGA 1 cut(s) 1241
TasI AATT 5 cut(s) 286, 338, 682, 1013, 1258
TatI WGTACW 1 cut(s) 1374
TauI GCSGC 3 cut(s) 942, 1011, 1139
TfiI GAWTC 4 cut(s) 570, 751, 864, 1292
Tru1I TTAA 6 cut(s) 57, 306, 582, 657, 702, 1221
Tru9I TTAA 6 cut(s) 57, 306, 582, 657, 702, 1221
TscAI CASTG 8 cut(s) 141, 384, 547, 901, 1075, 1287, 1340, 1476
TseFI GTSAC 4 cut(s) 132, 194, 469, 671
TseI GCWGC 2 cut(s) 399, 1403
Tsp45I GTSAC 4 cut(s) 132, 194, 469, 671
TspGWI ACGGA 1 cut(s) 1254
TspMI CCCGGG 1 cut(s) 1390
TspRI CASTG 8 cut(s) 141, 384, 547, 901, 1075, 1287, 1340, 1476
Van91I CCANNNNNTGG 1 cut(s) 518
VpaK11BI GGWCC 3 cut(s) 221, 291, 521
VspI ATTAAT 1 cut(s) 306
XceI RCATGY 2 cut(s) 68, 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmaI CCCGGG 1 cut(s) 1390
XmaJI CCTAGG 1 cut(s) 859
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 6 cut(s) 69, 75, 494, 567, 860, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.