FvH4_6g39890

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003423
Physical Location & Seq
Forward (+)
31511798 .. 31513442
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39890.t1

Sequence Viewer

Length: 774 bp
ATGGAAGGAAGCAGAAGCCTGACGAGCTTCCTTGAGCTCGATGCCATTCTAATCCGTAAGGTTGGAGCGGAGTTTTTGGGGACCTTCATTCTGATCTTTGCGGCAGCGGCAGGACCTATAGTGAACCAAAAGTACGATGGTGCTGAGACACTGCTAGGCAACGCAGCATGCTCAGGGTTAGCAGTGATGGTAGTGGTTTTTTCCACCGGCCACATCTCTGGAGCACACCTAAACCCATCGGTCACCGTCGCATTTGCAGCGTTTCGACACTTCCCTTGGTCCCAAGTTCCTCTCTACATTACAGCGCAGGTTTCGGCGTCCATCTCTGCAAGTTTTGCTCTAAAGTCTGTTTTCCATCCCTTTATGAACGGTGGAGTTACAGTTCCTTCAGTAGGCAATGGCCAAGCTTTTGCACTCGAGTTCATTGTCACTTTCATTCTCATGTTTGTAATCATTGCTGTTGCCACAGATACCCGTGCGGTAGGGGAGTTGGCAGGTGTAGCAATTGGAACTGCAGTATTGCTCGATATTCTTATAGCAGGGCCAACAACCGGAGCTTCAATGAATCCGGCCAGGACTTTGGGGCCGGCAATTGCTGCAGGACATTATAAATCGCTGTGGATCTACTTGGTTGCACCAACACTTGGTGCTTTAGCTGGTGCTGCTGCCTACACAGCTGTGAAGCTCCCAGAAATCCCACAAAAAACACAAATCCAGATCCATGACGACAGTAATGGTGAGGCTACGCCACAACCTCGCGTACATTTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

258

Amino Acids

26.7

Weight (kDa)

6.36

Isoelectric Point (pI)

24.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 18 - 224 1e-54 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AarI CACCTGC 1 cut(s) 485
Acc36I ACCTGC 2 cut(s) 298, 485
AccB7I CCANNNNNTGG 1 cut(s) 644
AccBSI CCGCTC 1 cut(s) 68
AccII CGCG 1 cut(s) 759
AciI CCGC 4 cut(s) 68, 101, 107, 479
AclWI GGATC 2 cut(s) 629, 712
AcoI YGGCCR 3 cut(s) 208, 400, 570
AcuI CTGAAG 1 cut(s) 372
AcyI GRCGYC 1 cut(s) 317
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 2 cut(s) 134, 762
AfiI CCNNNNNNNGG 3 cut(s) 392, 551, 644
AgsI TTSAA 1 cut(s) 561
AjnI CCWGG 1 cut(s) 572
AleI CACNNNNGTG 1 cut(s) 677
AloI GAACNNNNNNTCC 2 cut(s) 366, 398
AluBI AGCT 7 cut(s) 27, 37, 407, 557, 656, 677, 685
AluI AGCT 7 cut(s) 27, 37, 407, 557, 656, 677, 685
Alw21I GWGCWC 2 cut(s) 39, 226
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 2 cut(s) 629, 712
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 5 cut(s) 208, 400, 542, 570, 584
ApeKI GCWGC 6 cut(s) 104, 164, 257, 596, 662, 665
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 5 cut(s) 81, 113, 279, 542, 584
AsuHPI GGTGA 2 cut(s) 235, 749
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 3 cut(s) 81, 113, 279
BalI TGGCCA 1 cut(s) 402
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 39, 226
BbvI GCAGC 6 cut(s) 116, 176, 269, 583, 649, 652
BccI CCATC 5 cut(s) 131, 181, 244, 329, 363
BciT130I CCWGG 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 3 cut(s) 117, 513, 597
BfuAI ACCTGC 2 cut(s) 298, 485
BisI GCNGC 8 cut(s) 102, 105, 108, 165, 258, 597, 663, 666
BlsI GCNGC 8 cut(s) 103, 106, 109, 166, 259, 598, 664, 667
Bme1390I CCNGG 1 cut(s) 574
Bme18I GGWCC 3 cut(s) 81, 113, 279
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 5 cut(s) 81, 113, 279, 542, 584
BmiI GGNNCC 3 cut(s) 82, 281, 585
BmrFI CCNGG 1 cut(s) 574
BmsI GCATC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 172
BpuEI CTTGAG 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 275
BsaWI WCCGGW 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 551, 644
Bse118I RCCGGY 2 cut(s) 206, 586
Bse3DI GCAATG 2 cut(s) 403, 453
BseBI CCWGG 1 cut(s) 574
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 3 cut(s) 392, 551, 644
BseMI GCAATG 2 cut(s) 403, 453
BseMII CTCAG 2 cut(s) 135, 186
BseXI GCAGC 6 cut(s) 116, 176, 269, 583, 649, 652
Bsh1236I CGCG 1 cut(s) 759
BshFI GGCC 5 cut(s) 210, 402, 544, 572, 586
BsiHKAI GWGCWC 2 cut(s) 39, 226
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 4 cut(s) 207, 552, 569, 587
BslFI GGGAC 2 cut(s) 94, 265
BslI CCNNNNNNNGG 3 cut(s) 392, 551, 644
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 2 cut(s) 94, 265
BsnI GGCC 5 cut(s) 210, 402, 544, 572, 586
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 39, 226
Bsp143I GATC 3 cut(s) 93, 621, 717
BspACI CCGC 4 cut(s) 68, 101, 107, 479
BspANI GGCC 5 cut(s) 210, 402, 544, 572, 586
BspCNI CTCAG 2 cut(s) 136, 185
BspFNI CGCG 1 cut(s) 759
BspLI GGNNCC 3 cut(s) 82, 281, 585
BspMAI CTGCAG 2 cut(s) 517, 601
BspMI ACCTGC 2 cut(s) 298, 485
BspPI GGATC 2 cut(s) 629, 712
BsrBI CCGCTC 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 403, 453
BsrFI RCCGGY 2 cut(s) 206, 586
BssAI RCCGGY 2 cut(s) 206, 586
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 3 cut(s) 93, 621, 717
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 4 cut(s) 247, 371, 382, 731
BstACI GRCGYC 1 cut(s) 317
BstAPI GCANNNNNTGC 2 cut(s) 335, 596
BstC8I GCNNGC 2 cut(s) 169, 588
BstDEI CTNAG 2 cut(s) 144, 172
BstEII GGTNACC 1 cut(s) 241
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 759
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 3 cut(s) 96, 624, 720
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 3 cut(s) 93, 621, 717
BstMWI GCNNNNNNNGC 8 cut(s) 24, 107, 257, 335, 500, 596, 662, 674
BstNI CCWGG 1 cut(s) 574
BstNSI RCATGY 1 cut(s) 171
BstPI GGTNACC 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 572
BstSFI CTRYAG 3 cut(s) 117, 513, 597
BstUI CGCG 1 cut(s) 759
BstV1I GCAGC 6 cut(s) 116, 176, 269, 583, 649, 652
BstX2I RGATCY 2 cut(s) 621, 717
BstXI CCANNNNNNTGG 2 cut(s) 218, 580
BstYI RGATCY 2 cut(s) 621, 717
BsuRI GGCC 5 cut(s) 210, 402, 544, 572, 586
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 2 cut(s) 149, 189
BtsIMutI CAGTG 2 cut(s) 149, 189
BveI ACCTGC 2 cut(s) 298, 485
Cac8I GCNNGC 2 cut(s) 169, 588
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 2 cut(s) 206, 586
Cfr13I GGNCC 5 cut(s) 81, 113, 279, 542, 584
CseI GACGC 1 cut(s) 306
Csp6I GTAC 2 cut(s) 133, 761
CviAII CATG 3 cut(s) 168, 442, 722
CviQI GTAC 2 cut(s) 133, 761
DdeI CTNAG 2 cut(s) 144, 172
DpnI GATC 3 cut(s) 95, 623, 719
DpnII GATC 3 cut(s) 93, 621, 717
DraIII CACNNNGTG 1 cut(s) 647
EaeI YGGCCR 3 cut(s) 208, 400, 570
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 81, 113, 279
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 416
Eco91I GGTNACC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoO109I RGGNCCY 2 cut(s) 81, 113
EcoO65I GGTNACC 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 572
EcoT14I CCWWGG 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 3 cut(s) 171, 445, 725
FaiI YATR 7 cut(s) 119, 169, 365, 443, 536, 609, 723
FaqI GGGAC 2 cut(s) 94, 265
FatI CATG 3 cut(s) 167, 441, 721
Fnu4HI GCNGC 8 cut(s) 102, 105, 108, 165, 258, 597, 663, 666
FokI GGATG 1 cut(s) 342
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 8 cut(s) 102, 105, 108, 165, 258, 597, 663, 666
FspBI CTAG 1 cut(s) 155
GlaI GCGC 1 cut(s) 306
GluI GCNGC 8 cut(s) 102, 105, 108, 165, 258, 597, 663, 666
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 5 cut(s) 210, 402, 544, 572, 586
HapII CCGG 4 cut(s) 207, 552, 569, 587
HgaI GACGC 1 cut(s) 306
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 3 cut(s) 171, 445, 725
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 1 cut(s) 565
HpaII CCGG 4 cut(s) 207, 552, 569, 587
HphI GGTGA 2 cut(s) 235, 749
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 2 cut(s) 219, 715
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 2 cut(s) 94, 396
HpyCH4III ACNGT 4 cut(s) 247, 371, 382, 731
HpyCH4V TGCA 6 cut(s) 257, 329, 413, 515, 599, 635
HpyF10VI GCNNNNNNNGC 8 cut(s) 24, 107, 257, 335, 500, 596, 662, 674
HpyF3I CTNAG 2 cut(s) 144, 172
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 3 cut(s) 171, 445, 725
HspAI GCGC 1 cut(s) 305
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Kzo9I GATC 3 cut(s) 93, 621, 717
LmnI GCTCC 4 cut(s) 65, 221, 554, 690
Lsp1109I GCAGC 6 cut(s) 116, 176, 269, 583, 649, 652
LweI GCATC 1 cut(s) 31
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 3 cut(s) 241, 376, 427
MalI GATC 3 cut(s) 95, 623, 719
MbiI CCGCTC 1 cut(s) 68
MboI GATC 3 cut(s) 93, 621, 717
MfeI CAATTG 2 cut(s) 504, 591
MflI RGATCY 2 cut(s) 621, 717
MhlI GDGCHC 2 cut(s) 39, 226
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 2 cut(s) 504, 591
MluNI TGGCCA 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 3 cut(s) 300, 733, 765
Mox20I TGGCCA 1 cut(s) 402
MroNI GCCGGC 1 cut(s) 586
MscI TGGCCA 1 cut(s) 402
MslI CAYNNNNRTG 2 cut(s) 440, 677
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 107, 677
MspI CCGG 4 cut(s) 207, 552, 569, 587
MspR9I CCNGG 1 cut(s) 574
MunI CAATTG 2 cut(s) 504, 591
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 1 cut(s) 759
MwoI GCNNNNNNNGC 8 cut(s) 24, 107, 257, 335, 500, 596, 662, 674
NaeI GCCGGC 1 cut(s) 588
NdeII GATC 3 cut(s) 93, 621, 717
NgoMIV GCCGGC 1 cut(s) 586
NlaIII CATG 3 cut(s) 171, 445, 725
NlaIV GGNNCC 3 cut(s) 82, 281, 585
NmuCI GTSAC 2 cut(s) 241, 427
NspI RCATGY 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 677
PaeI GCATGC 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 416
PaqCI CACCTGC 1 cut(s) 485
PdiI GCCGGC 1 cut(s) 588
PfeI GAWTC 1 cut(s) 565
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 8 cut(s) 103, 106, 109, 166, 259, 598, 664, 667
PpuMI RGGWCCY 2 cut(s) 81, 113
PsiI TTATAA 1 cut(s) 609
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 2 cut(s) 81, 113
Psp6I CCWGG 1 cut(s) 572
PspEI GGTNACC 1 cut(s) 241
PspGI CCWGG 1 cut(s) 572
PspN4I GGNNCC 3 cut(s) 82, 281, 585
PspPI GGNCC 5 cut(s) 81, 113, 279, 542, 584
PspPPI RGGWCCY 2 cut(s) 81, 113
PspXI VCTCGAGB 1 cut(s) 416
PsrI GAACNNNNNNTAC 2 cut(s) 116, 148
PstI CTGCAG 2 cut(s) 517, 601
PsuI RGATCY 2 cut(s) 621, 717
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 134, 762
RsaNI GTAC 2 cut(s) 133, 761
RseI CAYNNNNRTG 2 cut(s) 440, 677
SacI GAGCTC 1 cut(s) 39
SatI GCNGC 8 cut(s) 102, 105, 108, 165, 258, 597, 663, 666
Sau3AI GATC 3 cut(s) 93, 621, 717
Sau96I GGNCC 5 cut(s) 81, 113, 279, 542, 584
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 2 cut(s) 39, 226
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 3 cut(s) 117, 513, 597
Sfr274I CTCGAG 1 cut(s) 416
SinI GGWCC 3 cut(s) 81, 113, 279
SlaI CTCGAG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 440, 677
SmlI CTYRAG 2 cut(s) 32, 416
SmoI CTYRAG 2 cut(s) 32, 416
SphI GCATGC 1 cut(s) 171
Sse9I AATT 2 cut(s) 504, 591
SsiI CCGC 4 cut(s) 68, 101, 107, 479
SspMI CTAG 1 cut(s) 155
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 572
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 4 cut(s) 247, 371, 382, 731
TaqI TCGA 4 cut(s) 39, 265, 417, 525
TaqII GACCGA 1 cut(s) 229
TasI AATT 2 cut(s) 504, 591
TauI GCSGC 2 cut(s) 104, 110
TfiI GAWTC 1 cut(s) 565
TscAI CASTG 2 cut(s) 156, 189
TseFI GTSAC 2 cut(s) 241, 427
TseI GCWGC 6 cut(s) 104, 164, 257, 596, 662, 665
Tsp45I GTSAC 2 cut(s) 241, 427
TspDTI ATGAA 5 cut(s) 76, 380, 412, 424, 578
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 2 cut(s) 156, 189
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 81, 113, 279
XagI CCTNNNNNAGG 1 cut(s) 390
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 416
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.