RchiOBHm_Chr2g0154421

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003423
Physical Location & Seq
Forward (+)
71604342 .. 71605444
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52341

Sequence Viewer

Length: 420 bp
ATGAACGGTGGAGTTACAATTCCTTCAGTAGGTAATGGCCAAGCTTTTGCACGCGAGTTTATTGTCACTTTCAACCTCATGTTTGTCATCATTTTTGTTGCCACAGATACCGCGCCATGCGGTTTAGCAATTGGAGCTACAGTATTGCTCGACATTCTTATAGCAGGGAAAACAACCGGCGCTTCAATGAATCCGGTCCGGACTTTGGGGCCGACAGTGGCTGCAGGACATTATAAGTCACTGTGGATCTACTTGGTTGCCCCAAGACTTGGTGCTTTAGCTGGTGCTGCTGCCCACACAGCTGCGAAGCTCTCAGAAATCCGACCAAACCAGCAGATCCATGATGCCAGTAATGATGAGACTACACCACAACCTCGCATTCATTTCCCCCCAGACAAATCTCCTTACCGGACAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

139

Amino Acids

14.61

Weight (kDa)

8.93

Isoelectric Point (pI)

21.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 7 - 98 1.6e-20 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 269
AccII CGCG 2 cut(s) 54, 113
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 2 cut(s) 111, 120
AclWI GGATC 2 cut(s) 254, 331
AcoI YGGCCR 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 9
AfiI CCNNNNNNNGG 3 cut(s) 29, 205, 269
AgsI TTSAA 2 cut(s) 73, 186
AluBI AGCT 5 cut(s) 44, 137, 281, 302, 310
AluI AGCT 5 cut(s) 44, 137, 281, 302, 310
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 2 cut(s) 254, 331
AlwNI CAGNNNCTG 1 cut(s) 221
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 2 cut(s) 37, 209
ApeKI GCWGC 4 cut(s) 221, 287, 290, 302
AspLEI GCGC 2 cut(s) 115, 182
AspS9I GGNCC 2 cut(s) 196, 209
AvaII GGWCC 1 cut(s) 196
BalI TGGCCA 1 cut(s) 39
BbvI GCAGC 4 cut(s) 208, 274, 277, 289
BcoDI GTCTC 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 138, 222
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 4 cut(s) 222, 288, 291, 303
BlsI GCNGC 4 cut(s) 223, 289, 292, 304
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 196, 209
BmiI GGNNCC 1 cut(s) 210
BmsI GCATC 1 cut(s) 334
BsaWI WCCGGW 3 cut(s) 193, 198, 408
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 205, 269
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 29, 205, 269
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 348
BseXI GCAGC 4 cut(s) 208, 274, 277, 289
Bsh1236I CGCG 2 cut(s) 54, 113
BshFI GGCC 2 cut(s) 39, 211
BsiSI CCGG 4 cut(s) 177, 194, 199, 409
BslI CCNNNNNNNGG 3 cut(s) 29, 205, 269
BsmAI GTCTC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 2 cut(s) 39, 211
Bsp13I TCCGGA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 246, 336
BspACI CCGC 2 cut(s) 111, 120
BspANI GGCC 2 cut(s) 39, 211
BspCNI CTCAG 1 cut(s) 326
BspEI TCCGGA 1 cut(s) 198
BspFNI CGCG 2 cut(s) 54, 113
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 2 cut(s) 254, 331
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 348
BssAI RCCGGY 1 cut(s) 176
BssMI GATC 2 cut(s) 246, 336
Bst4CI ACNGT 4 cut(s) 8, 142, 217, 243
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 1 cut(s) 313
BstENI CCTNNNNNAGG 1 cut(s) 27
BstFNI CGCG 2 cut(s) 54, 113
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 115, 182
BstKTI GATC 2 cut(s) 249, 339
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 2 cut(s) 246, 336
BstMWI GCNNNNNNNGC 3 cut(s) 134, 287, 299
BstSFI CTRYAG 2 cut(s) 138, 222
BstUI CGCG 2 cut(s) 54, 113
BstV1I GCAGC 4 cut(s) 208, 274, 277, 289
BstX2I RGATCY 2 cut(s) 246, 336
BstYI RGATCY 2 cut(s) 246, 336
BsuRI GGCC 2 cut(s) 39, 211
BtsIMutI CAGTG 2 cut(s) 222, 239
Cac8I GCNNGC 1 cut(s) 52
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 2 cut(s) 115, 182
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 196, 209
CpoI CGGWCCG 1 cut(s) 196
CspI CGGWCCG 1 cut(s) 196
CviAII CATG 3 cut(s) 79, 117, 341
CviJI RGCY 8 cut(s) 39, 44, 137, 211, 221, 281, 302, 310
CviKI_1 RGCY 8 cut(s) 39, 44, 137, 211, 221, 281, 302, 310
DdeI CTNAG 1 cut(s) 313
DpnI GATC 2 cut(s) 248, 338
DpnII GATC 2 cut(s) 246, 336
EaeI YGGCCR 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 27
FaeI CATG 3 cut(s) 82, 120, 344
FaiI YATR 5 cut(s) 80, 118, 161, 234, 342
FatI CATG 3 cut(s) 78, 116, 340
Fnu4HI GCNGC 4 cut(s) 222, 288, 291, 303
Fsp4HI GCNGC 4 cut(s) 222, 288, 291, 303
GlaI GCGC 2 cut(s) 114, 181
GluI GCNGC 4 cut(s) 222, 288, 291, 303
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 39, 211
HapII CCGG 4 cut(s) 177, 194, 199, 409
HhaI GCGC 2 cut(s) 115, 182
Hin1II CATG 3 cut(s) 82, 120, 344
Hin6I GCGC 2 cut(s) 113, 180
HinP1I GCGC 2 cut(s) 113, 180
HindIII AAGCTT 1 cut(s) 42
HinfI GANTC 1 cut(s) 190
HpaII CCGG 4 cut(s) 177, 194, 199, 409
Hpy188I TCNGA 2 cut(s) 316, 323
Hpy188III TCNNGA 1 cut(s) 199
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 4 cut(s) 8, 142, 217, 243
HpyCH4V TGCA 2 cut(s) 50, 224
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 287, 299
HpyF3I CTNAG 1 cut(s) 313
Hsp92II CATG 3 cut(s) 82, 120, 344
HspAI GCGC 2 cut(s) 113, 180
Kpn2I TCCGGA 1 cut(s) 198
Kzo9I GATC 2 cut(s) 246, 336
LmnI GCTCC 1 cut(s) 134
Lsp1109I GCAGC 4 cut(s) 208, 274, 277, 289
LweI GCATC 1 cut(s) 334
MaeIII GTNAC 3 cut(s) 13, 64, 237
MalI GATC 2 cut(s) 248, 338
MboI GATC 2 cut(s) 246, 336
MfeI CAATTG 1 cut(s) 129
MflI RGATCY 2 cut(s) 246, 336
MlsI TGGCCA 1 cut(s) 39
MluCI AATT 2 cut(s) 18, 129
MluNI TGGCCA 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 2 cut(s) 86, 384
Mox20I TGGCCA 1 cut(s) 39
MroI TCCGGA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 39
Msp20I TGGCCA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 4 cut(s) 177, 194, 199, 409
MunI CAATTG 1 cut(s) 129
Mva1269I GAATGC 1 cut(s) 378
MvnI CGCG 2 cut(s) 54, 113
MwoI GCNNNNNNNGC 3 cut(s) 134, 287, 299
NdeII GATC 2 cut(s) 246, 336
NlaIII CATG 3 cut(s) 82, 120, 344
NlaIV GGNNCC 1 cut(s) 210
NmuCI GTSAC 2 cut(s) 64, 237
PctI GAATGC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 190
PflMI CCANNNNNTGG 1 cut(s) 269
PkrI GCNGC 4 cut(s) 223, 289, 292, 304
PsiI TTATAA 1 cut(s) 234
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 2 cut(s) 196, 209
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 221
PsuI RGATCY 2 cut(s) 246, 336
PvuII CAGCTG 1 cut(s) 302
Rsr2I CGGWCCG 1 cut(s) 196
RsrII CGGWCCG 1 cut(s) 196
SatI GCNGC 4 cut(s) 222, 288, 291, 303
Sau3AI GATC 2 cut(s) 246, 336
Sau96I GGNCC 2 cut(s) 196, 209
SetI ASST 8 cut(s) 34, 46, 78, 139, 283, 304, 312, 376
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 2 cut(s) 138, 222
SinI GGWCC 1 cut(s) 196
Sse9I AATT 2 cut(s) 18, 129
SsiI CCGC 2 cut(s) 111, 120
TaaI ACNGT 4 cut(s) 8, 142, 217, 243
TaqI TCGA 1 cut(s) 150
TasI AATT 2 cut(s) 18, 129
TfiI GAWTC 1 cut(s) 190
TscAI CASTG 2 cut(s) 222, 246
TseFI GTSAC 2 cut(s) 64, 237
TseI GCWGC 4 cut(s) 221, 287, 290, 302
Tsp45I GTSAC 2 cut(s) 64, 237
TspDTI ATGAA 3 cut(s) 17, 203, 371
TspRI CASTG 2 cut(s) 222, 246
Van91I CCANNNNNTGG 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 196
XagI CCTNNNNNAGG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.