MD09G1135700.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003423
Physical Location & Seq
Reverse (-)
10421534 .. 10422029
496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1135700.v1.1.491

Sequence Viewer

Length: 336 bp
ATGTTTATTATCACTGCCGTTGCCATTGATACCCGTGCGGTGGGGGAGATGGCAGGCGTAGCAATTGGAGCTACAGTTTTACTCAACATTCTTGTGGCAGGACCAACAACTGGCGGTTCGATGAACCCTATCCAAACTTTGGGTCCGGGTGTTGCTACCGGAAATTATAACGGATTTTGTATCTGCTTAGTTGCACCCCCTCTTGGTGCCTTAGCTGGTGCTGCTGCCTACACAATTGTGAAGCTCCTAGACAATAAACCACCAAACCAGGTTAGAACTTCCCTTAATACTGCACCTACGAAAAAAATTATCAGATTCATGGAAGACCCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

112

Amino Acids

11.34

Weight (kDa)

7.77

Isoelectric Point (pI)

14.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 1 - 77 3.1e-14 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 206
AccB7I CCANNNNNTGG 2 cut(s) 110, 139
AciI CCGC 2 cut(s) 38, 114
AfiI CCNNNNNNNGG 4 cut(s) 40, 110, 139, 203
AjnI CCWGG 1 cut(s) 267
AleI CACNNNNGTG 1 cut(s) 236
AluBI AGCT 3 cut(s) 71, 215, 244
AluI AGCT 3 cut(s) 71, 215, 244
ApeKI GCWGC 2 cut(s) 221, 224
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspS9I GGNCC 2 cut(s) 101, 143
AsuC2I CCSGG 1 cut(s) 147
AvaII GGWCC 2 cut(s) 101, 143
BanI GGYRCC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 208, 211
BccI CCATC 1 cut(s) 43
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 72
BisI GCNGC 2 cut(s) 222, 225
BlsI GCNGC 2 cut(s) 223, 226
Bme1390I CCNGG 2 cut(s) 147, 269
Bme18I GGWCC 2 cut(s) 101, 143
BmgT120I GGNCC 2 cut(s) 101, 143
BmiI GGNNCC 2 cut(s) 144, 208
BmrFI CCNGG 2 cut(s) 147, 269
BpiI GAAGAC 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 211
BpuMI CCSGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 110, 139, 203
Bse1I ACTGG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 269
BseLI CCNNNNNNNGG 4 cut(s) 40, 110, 139, 203
BseNI ACTGG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 208, 211
BsgI GTGCAG 1 cut(s) 276
BshNI GGYRCC 1 cut(s) 206
BsiSI CCGG 2 cut(s) 146, 159
BslI CCNNNNNNNGG 4 cut(s) 40, 110, 139, 203
BspACI CCGC 2 cut(s) 38, 114
BspLI GGNNCC 2 cut(s) 144, 208
BspT107I GGYRCC 1 cut(s) 206
BsrI ACTGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 187, 211
BstMWI GCNNNNNNNGC 3 cut(s) 59, 68, 221
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 145, 267
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 2 cut(s) 208, 211
BstV2I GAAGAC 1 cut(s) 330
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 101, 143
CsiI ACCWGGT 1 cut(s) 267
CviAII CATG 1 cut(s) 319
CviJI RGCY 3 cut(s) 71, 215, 244
CviKI_1 RGCY 3 cut(s) 71, 215, 244
DdeI CTNAG 2 cut(s) 187, 211
Eco47I GGWCC 2 cut(s) 101, 143
EcoRII CCWGG 1 cut(s) 267
FaeI CATG 1 cut(s) 322
FaiI YATR 2 cut(s) 168, 320
FatI CATG 1 cut(s) 318
Fnu4HI GCNGC 2 cut(s) 222, 225
Fsp4HI GCNGC 2 cut(s) 222, 225
FspBI CTAG 1 cut(s) 248
GluI GCNGC 2 cut(s) 222, 225
HapII CCGG 2 cut(s) 146, 159
Hin1II CATG 1 cut(s) 322
HinfI GANTC 1 cut(s) 315
HpaII CCGG 2 cut(s) 146, 159
Hpy188I TCNGA 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 194, 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 68, 221
HpyF3I CTNAG 2 cut(s) 187, 211
Hsp92II CATG 1 cut(s) 322
LmnI GCTCC 2 cut(s) 68, 249
LpnPI CCDG 8 cut(s) 39, 84, 96, 159, 172, 201, 254, 281
Lsp1109I GCAGC 2 cut(s) 208, 211
MabI ACCWGGT 1 cut(s) 267
MaeI CTAG 1 cut(s) 248
MboII GAAGA 1 cut(s) 335
MfeI CAATTG 2 cut(s) 63, 234
MluCI AATT 4 cut(s) 63, 163, 234, 306
MnlI CCTC 1 cut(s) 210
MseI TTAA 2 cut(s) 285, 334
MslI CAYNNNNRTG 2 cut(s) 92, 236
MspI CCGG 2 cut(s) 146, 159
MspR9I CCNGG 2 cut(s) 147, 269
MunI CAATTG 2 cut(s) 63, 234
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 59, 68, 221
NciI CCSGG 1 cut(s) 147
NlaIII CATG 1 cut(s) 322
NlaIV GGNNCC 2 cut(s) 144, 208
OliI CACNNNNGTG 1 cut(s) 236
PfeI GAWTC 1 cut(s) 315
PflMI CCANNNNNTGG 2 cut(s) 110, 139
PkrI GCNGC 2 cut(s) 223, 226
PsiI TTATAA 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 144, 208
PspPI GGNCC 2 cut(s) 101, 143
RseI CAYNNNNRTG 2 cut(s) 92, 236
SaqAI TTAA 2 cut(s) 285, 334
SatI GCNGC 2 cut(s) 222, 225
Sau96I GGNCC 2 cut(s) 101, 143
ScrFI CCNGG 2 cut(s) 147, 269
SetI ASST 5 cut(s) 73, 217, 246, 273, 298
SexAI ACCWGGT 1 cut(s) 267
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 2 cut(s) 101, 143
SmiMI CAYNNNNRTG 2 cut(s) 92, 236
Sse9I AATT 4 cut(s) 63, 163, 234, 306
SsiI CCGC 2 cut(s) 38, 114
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 145, 267
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 1 cut(s) 119
TasI AATT 4 cut(s) 63, 163, 234, 306
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 2 cut(s) 285, 334
Tru9I TTAA 2 cut(s) 285, 334
TscAI CASTG 1 cut(s) 19
TseI GCWGC 2 cut(s) 221, 224
TspDTI ATGAA 2 cut(s) 137, 307
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 2 cut(s) 110, 139
VpaK11BI GGWCC 2 cut(s) 101, 143
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.