MD09G1135600.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003423
Physical Location & Seq
Reverse (-)
10411135 .. 10412157
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1135600.v1.1.491

Sequence Viewer

Length: 744 bp
ATGCATGTGTATTTACAGCTTGGAGCCGAGTTTGTGGGGAGCTTCATCCTGATATTTTCAGCAGCAGCAGCCCCTATTGTGAACCAAAAGTATCAAGGTTCGGAGACGCTGCTAGGCAATGCGGCATGCGCAGGCCTAGCAGTGATGGCAGTGATTTTGTCCACAGGCCACATCTCCGGAGCACACCTAAACCCGTCTGTCACCATTGCTTTTGCAACGTTTCGACACTTCCCATGGACCCAAGTTCCCCTCTACATAATAGCACAGGTTTCAGCATCCATCTCTGCAAGTTTCGCTCTGAAGGGTGTTTTTCATCCATTCATGTCTGGTGGAGTAACGGTTCCTTCTGTTAGCCATTGCCAAGCTTTTGCACTCGAGTTCATTGTCACTTTCATTCTCATGTTCACTGTCACTTCCGTTGCCACTGATACTCGTGCGGTGGGGGAGTTGGCAGGTGTAGCAATTGGAGCGACAGTTTTGCTCAACATTCTTGTGGCAGGACCAACAACGGGCGGCTCAATGAACCCTATCCGGACTTTGGGTCCGGCTGTGGCTGCCGGAAACTATAAGGGATTGTGGATCTATTTGGTTGCACCCACACTTGGTGCCTTAGCTGGTGCTGCTACGTACACAGCTGTGAAGCTCCCACACAACCAGATTCGTGACAAGGCTGATGGCGGCACTCCCTTGCCGCAATTCTACTCAGACGGATCTCCTTCCATCGAACAGGGTAATTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

248

Amino Acids

25.52

Weight (kDa)

7.05

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 6 - 210 7.4e-51 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 443
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 605
AccIII TCCGGA 2 cut(s) 176, 531
AciI CCGC 5 cut(s) 122, 437, 513, 678, 692
AclI AACGTT 1 cut(s) 218
AclWI GGATC 2 cut(s) 587, 718
AcuI CTGAAG 1 cut(s) 320
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 509, 538, 602
AgsI TTSAA 1 cut(s) 738
AhdI GACNNNNNGTC 1 cut(s) 540
AleI CACNNNNGTG 1 cut(s) 635
AloI GAACNNNNNNTCC 2 cut(s) 324, 356
AluBI AGCT 6 cut(s) 19, 42, 365, 614, 635, 643
AluI AGCT 6 cut(s) 19, 42, 365, 614, 635, 643
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 2 cut(s) 587, 718
Ama87I CYCGRG 1 cut(s) 374
Aor13HI TCCGGA 2 cut(s) 176, 531
AoxI GGCC 2 cut(s) 133, 166
ApeKI GCWGC 6 cut(s) 62, 65, 68, 109, 554, 620
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 237, 500, 542
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 3 cut(s) 237, 500, 542
BanI GGYRCC 1 cut(s) 605
BauI CACGAG 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 6 cut(s) 74, 77, 80, 96, 541, 607
BccI CCATC 4 cut(s) 139, 287, 668, 728
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 3 cut(s) 113, 137, 742
BfuAI ACCTGC 1 cut(s) 443
Bme18I GGWCC 3 cut(s) 237, 500, 542
BmeRI GACNNNNNGTC 1 cut(s) 540
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 3 cut(s) 237, 500, 542
BmiI GGNNCC 5 cut(s) 25, 239, 342, 543, 607
BmsI GCATC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 627
BsaJI CCNNGG 1 cut(s) 233
BsaWI WCCGGW 2 cut(s) 176, 531
BsaXI ACNNNNNCTCC 4 cut(s) 324, 354, 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 509, 538, 602
Bse3DI GCAATG 3 cut(s) 124, 204, 355
BseAI TCCGGA 2 cut(s) 176, 531
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 3 cut(s) 45, 275, 313
BseLI CCNNNNNNNGG 3 cut(s) 509, 538, 602
BseMI GCAATG 3 cut(s) 124, 204, 355
BseMII CTCAG 1 cut(s) 717
BseXI GCAGC 6 cut(s) 74, 77, 80, 96, 541, 607
BshFI GGCC 2 cut(s) 135, 168
BshNI GGYRCC 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 4 cut(s) 177, 532, 545, 558
BslI CCNNNNNNNGG 3 cut(s) 509, 538, 602
BsmAI GTCTC 1 cut(s) 98
BsmBI CGTCTC 1 cut(s) 98
BsnI GGCC 2 cut(s) 135, 168
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 184
Bsp13I TCCGGA 2 cut(s) 176, 531
Bsp143I GATC 2 cut(s) 579, 710
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 5 cut(s) 122, 437, 513, 678, 692
BspANI GGCC 2 cut(s) 135, 168
BspCNI CTCAG 1 cut(s) 716
BspEI TCCGGA 2 cut(s) 176, 531
BspLI GGNNCC 5 cut(s) 25, 239, 342, 543, 607
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 2 cut(s) 587, 718
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 3 cut(s) 124, 204, 355
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 2 cut(s) 579, 710
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 233
Bst2BI CACGAG 1 cut(s) 432
Bst4CI ACNGT 3 cut(s) 340, 409, 475
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 127, 133
BstDEI CTNAG 2 cut(s) 610, 703
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 3 cut(s) 45, 275, 313
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 582, 713
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 2 cut(s) 579, 710
BstMWI GCNNNNNNNGC 9 cut(s) 68, 128, 137, 146, 293, 458, 467, 554, 620
BstNSI RCATGY 2 cut(s) 8, 129
BstSNI TACGTA 1 cut(s) 627
BstV1I GCAGC 6 cut(s) 74, 77, 80, 96, 541, 607
BstX2I RGATCY 2 cut(s) 579, 710
BstYI RGATCY 2 cut(s) 579, 710
BsuRI GGCC 2 cut(s) 135, 168
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 3 cut(s) 45, 275, 313
BtsI GCAGTG 2 cut(s) 147, 156
BtsIMutI CAGTG 4 cut(s) 147, 156, 405, 423
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 2 cut(s) 127, 133
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 237, 500, 542
CseI GACGC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 628
CviAII CATG 5 cut(s) 5, 126, 234, 322, 400
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 2 cut(s) 610, 703
DpnI GATC 2 cut(s) 581, 712
DpnII GATC 2 cut(s) 579, 710
DraIII CACNNNGTG 1 cut(s) 605
DriI GACNNNNNGTC 1 cut(s) 540
Eam1105I GACNNNNNGTC 1 cut(s) 540
Eco105I TACGTA 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 233
Eco147I AGGCCT 1 cut(s) 135
Eco47I GGWCC 3 cut(s) 237, 500, 542
Eco57I CTGAAG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 233
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 5 cut(s) 8, 129, 237, 325, 403
FaiI YATR 7 cut(s) 6, 127, 235, 257, 323, 401, 567
FatI CATG 5 cut(s) 4, 125, 233, 321, 399
FokI GGATG 3 cut(s) 32, 262, 300
FspBI CTAG 3 cut(s) 113, 137, 742
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 1 cut(s) 130
HaeIII GGCC 2 cut(s) 135, 168
HapII CCGG 4 cut(s) 177, 532, 545, 558
HgaI GACGC 1 cut(s) 115
HhaI GCGC 1 cut(s) 131
Hin1II CATG 5 cut(s) 8, 129, 237, 325, 403
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 1 cut(s) 658
HpaII CCGG 4 cut(s) 177, 532, 545, 558
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 4 cut(s) 82, 162, 405, 630
Hpy188I TCNGA 3 cut(s) 103, 300, 706
Hpy188III TCNNGA 4 cut(s) 49, 177, 532, 662
Hpy8I GTNNAC 4 cut(s) 82, 162, 405, 630
HpyAV CCTTC 3 cut(s) 295, 354, 726
HpyCH4III ACNGT 3 cut(s) 340, 409, 475
HpyCH4IV ACGT 2 cut(s) 218, 626
HpyCH4V TGCA 5 cut(s) 4, 215, 287, 371, 593
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 128, 137, 146, 293, 458, 467, 554, 620
HpyF3I CTNAG 2 cut(s) 610, 703
HpySE526I ACGT 2 cut(s) 218, 626
Hsp92II CATG 5 cut(s) 8, 129, 237, 325, 403
HspAI GCGC 1 cut(s) 129
Kpn2I TCCGGA 2 cut(s) 176, 531
Kzo9I GATC 2 cut(s) 579, 710
LmnI GCTCC 5 cut(s) 23, 39, 179, 467, 648
Lsp1109I GCAGC 6 cut(s) 74, 77, 80, 96, 541, 607
LweI GCATC 1 cut(s) 284
MaeI CTAG 3 cut(s) 113, 137, 742
MaeII ACGT 2 cut(s) 218, 626
MaeIII GTNAC 5 cut(s) 199, 334, 385, 409, 662
MalI GATC 2 cut(s) 581, 712
MboI GATC 2 cut(s) 579, 710
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 579, 710
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 3 cut(s) 462, 695, 733
MnlI CCTC 1 cut(s) 260
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 2 cut(s) 176, 531
MslI CAYNNNNRTG 3 cut(s) 398, 491, 635
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 4 cut(s) 177, 532, 545, 558
MunI CAATTG 1 cut(s) 462
MwoI GCNNNNNNNGC 9 cut(s) 68, 128, 137, 146, 293, 458, 467, 554, 620
NcoI CCATGG 1 cut(s) 233
NdeII GATC 2 cut(s) 579, 710
NlaIII CATG 5 cut(s) 8, 129, 237, 325, 403
NlaIV GGNNCC 5 cut(s) 25, 239, 342, 543, 607
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 4 cut(s) 199, 385, 409, 662
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 129
OliI CACNNNNGTG 1 cut(s) 635
PaeI GCATGC 1 cut(s) 129
PaeR7I CTCGAG 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 443
PceI AGGCCT 1 cut(s) 135
PfeI GAWTC 1 cut(s) 658
Ppu21I YACGTR 1 cut(s) 627
Psp1406I AACGTT 1 cut(s) 218
PspN4I GGNNCC 5 cut(s) 25, 239, 342, 543, 607
PspPI GGNCC 3 cut(s) 237, 500, 542
PspXI VCTCGAGB 1 cut(s) 374
PsuI RGATCY 2 cut(s) 579, 710
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 3 cut(s) 398, 491, 635
Sau3AI GATC 2 cut(s) 579, 710
Sau96I GGNCC 3 cut(s) 237, 500, 542
SduI GDGCHC 1 cut(s) 184
SfaNI GCATC 1 cut(s) 284
Sfr274I CTCGAG 1 cut(s) 374
SinI GGWCC 3 cut(s) 237, 500, 542
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 3 cut(s) 398, 491, 635
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SnaBI TACGTA 1 cut(s) 627
SphI GCATGC 1 cut(s) 129
Sse9I AATT 3 cut(s) 462, 695, 733
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 5 cut(s) 122, 437, 513, 678, 692
SspMI CTAG 3 cut(s) 113, 137, 742
StuI AGGCCT 1 cut(s) 135
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 3 cut(s) 340, 409, 475
TaiI ACGT 2 cut(s) 221, 629
TaqI TCGA 3 cut(s) 223, 375, 723
TasI AATT 3 cut(s) 462, 695, 733
TauI GCSGC 4 cut(s) 125, 516, 681, 694
TfiI GAWTC 1 cut(s) 658
TscAI CASTG 4 cut(s) 147, 156, 412, 430
TseFI GTSAC 4 cut(s) 199, 385, 409, 662
TseI GCWGC 6 cut(s) 62, 65, 68, 109, 554, 620
Tsp45I GTSAC 4 cut(s) 199, 385, 409, 662
TspDTI ATGAA 6 cut(s) 34, 302, 310, 370, 382, 536
TspGWI ACGGA 2 cut(s) 406, 723
TspRI CASTG 4 cut(s) 147, 156, 412, 430
VpaK11BI GGWCC 3 cut(s) 237, 500, 542
XceI RCATGY 2 cut(s) 8, 129
XhoI CTCGAG 1 cut(s) 374
XspI CTAG 3 cut(s) 113, 137, 742
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.