Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003423

Physical Location & Seq

Genomic Coordinates Forward (+)

23697369 .. 23707092

Gene Structure 9724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1144100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGGCGGAATCTGAGCCGGTGACGCCATCGGCTTCGGCGCCGGCGACGCCGGGAACGCCACTGCCTCTGTTTTCAGAGCCGAGAGTGGATTCGCTGTCGTATGAGCGTAAGTCAATGCCGCGAACCAAGTGCTTGCCTGTGATAGCTCCGACGTGGGGTCAATCTCCCAACTGCTTCAACGAGTTCCCTACTCCAACTGTCTCCCTCAGCCGCAAGGTAGGAGCTGAGTTCGTAGGAACCTTCATCTTGATGTTTGCAGCAAGTGCTGGACCAATCGTGAACCAAAAGTACAACGGAGCGGAGTCCTTGATCGGAAATGCAGCATGCGCAGGGCTTGGTGTGATGATTGTGATTCTCTCCACAGGCCACATTTCTGGTGCACATCTAAACCCATCTCTCACCATCGCGTTTGCCGCCCTGCGACATTTCCCATGGCTTCATGTCCCTGCCTACATCGCAGCTCAGGTTTTGGGCTCCATTTGCGCCTCGTTTACTCTCAAAGGGGTTTTACATCCGTACATGTCTGGCGGAGTCACGGTGCCTACTGTGAGCACTGGCCAGGCTTTTGGACTTGAGTTCATCATCACTTTTAATCTCTTGTTTGTTGTCACTGCTGTTGCAACTGACACTCGAGCGTTGGGAGAGTTGGCTGGTTTAGCAGTTGGGGCTTGTGTCATGCTAAACATTCTTATGGCAGGGCCATCTAGTGGTGGTTCAATGAACCCGGTGCGTACTCTCGGCCCTGCTATCGCTGCCGGAAACTACACCAAGTTGTGGATATACCTGGTGGCTCCTACCCTTGGAGCTGTCGCCGGGGCAGGTACCTATACAGCTGTGAAGCTTCGAGAAGATGAGGTGGATGCACCTGTGCGCATGGCCAGGAGCTTCCGTCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005372 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006833 GO:0006855 GO:0007154 GO:0008150 GO:0008509 GO:0009605 GO:0009987 GO:0009991 GO:0010036 GO:0015075 GO:0015103 GO:0015105 GO:0015238 GO:0015250 GO:0015267 GO:0015318 GO:0015698 GO:0015700 GO:0015893 GO:0016020 GO:0016021 GO:0016328 GO:0022803 GO:0022804 GO:0022838 GO:0022857 GO:0031224 GO:0031226 GO:0031667 GO:0031668 GO:0031669 GO:0034220 GO:0035445 GO:0042044 GO:0042221 GO:0042493 GO:0044425 GO:0044459 GO:0044464 GO:0046685 GO:0046713 GO:0046715 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0070887 GO:0071496 GO:0071944 GO:0080029 GO:0098656 GO:0098660 GO:0098661

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MIP

Protein Analysis

299

Amino Acids

31.05

Weight (kDa)

8.85

Isoelectric Point (pI)

40.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIP	PF00230	66 - 276	7.2e-53	Major intrinsic protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10380
fragaria_vesca	FvH4_5g10070 FvH4_6g39890 FvH4_6g39890
malus_domestica	MD06G1128200.v1.1 MD09G1135600.v1.1 MD09G1135700.v1.1 MD14G1144100.v1.1
prunus_persica	Prupe.3G191000_v2.0.a1 Prupe.5G138000_v2.0.a1 Prupe.5G138000_v2.0.a1 Prupe.5G138000_v2.0.a1
pyrus_communis	pycom06g11840 pycom09g05660
rosa_chinensis	RchiOBHm_Chr2g0154411 RchiOBHm_Chr2g0154421 RchiOBHm_Chr7g0188201
rosa_laevigata	RLG00000004719 RLG00000020776
rosa_multiflora	Rmu_co8400175.1_g000001 Rmu_sc0002519.1_g000004 Rmu_sc0008750.1_g000017
rosa_roxburghii	Rroxscaffold_2G00094350 Rroxscaffold_3G00266670
rosa_rugosa	Rorug07G0277000 Rorug07G0277100
rosa_samantha	Rh2AG508700 Rh2BG519900 Rh2BG520000 Rh2CG494100 Rh2DG530300 Rh7AG093400 Rh7BG094900 Rh7DG095300
rosa_wichuraiana	Rw2G041770 Rw7G008050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	328, 872
Acc36I	ACCTGC	1 cut(s)	809
Acc65I	GGTACC	1 cut(s)	821
AccB1I	GGYRCC	3 cut(s)	37, 538, 821
AccB7I	CCANNNNNTGG	2 cut(s)	707, 774
AccBSI	CCGCTC	1 cut(s)	299
AccII	CGCG	2 cut(s)	121, 407
AciI	CCGC	6 cut(s)	5, 119, 211, 299, 414, 528
AcoI	YGGCCR	2 cut(s)	556, 876
AcyI	GRCGYC	3 cut(s)	23, 38, 47
AfaI	GTAC	4 cut(s)	290, 518, 733, 823
AfiI	CCNNNNNNNGG	4 cut(s)	155, 707, 774, 800
AflIII	ACRYGT	1 cut(s)	519
AgsI	TTSAA	2 cut(s)	178, 717
AhdI	GACNNNNNGTC	1 cut(s)	156
AjiI	CACGTC	1 cut(s)	153
AjnI	CCWGG	3 cut(s)	558, 783, 878
AluBI	AGCT	7 cut(s)	146, 224, 461, 806, 833, 841, 885
AluI	AGCT	7 cut(s)	146, 224, 461, 806, 833, 841, 885
Alw21I	GWGCWC	2 cut(s)	382, 554
Alw26I	GTCTC	1 cut(s)	205
Alw44I	GTGCAC	1 cut(s)	378
Ama87I	CYCGRG	1 cut(s)	630
AoxI	GGCC	5 cut(s)	364, 556, 698, 739, 876
ApaLI	GTGCAC	1 cut(s)	378
ApeKI	GCWGC	4 cut(s)	257, 320, 458, 752
Asp718I	GGTACC	1 cut(s)	821
AspLEI	GCGC	4 cut(s)	40, 329, 485, 873
AspS9I	GGNCC	3 cut(s)	269, 698, 740
AsuC2I	CCSGG	3 cut(s)	51, 725, 814
AsuHPI	GGTGA	2 cut(s)	31, 391
AvaI	CYCGRG	1 cut(s)	630
AvaII	GGWCC	1 cut(s)	269
BaeGI	GKGCMC	1 cut(s)	382
BalI	TGGCCA	2 cut(s)	558, 878
BanI	GGYRCC	3 cut(s)	37, 538, 821
BanII	GRGCYC	1 cut(s)	476
Bbv12I	GWGCWC	2 cut(s)	382, 554
BbvCI	CCTCAGC	1 cut(s)	206
BbvI	GCAGC	4 cut(s)	269, 332, 470, 739
BccI	CCATC	4 cut(s)	34, 400, 410, 709
BcgI	CGANNNNNNTGC	2 cut(s)	111, 145
BciT130I	CCWGG	3 cut(s)	560, 785, 880
BcnI	CCSGG	3 cut(s)	51, 725, 814
BcoDI	GTCTC	1 cut(s)	205
BfaI	CTAG	2 cut(s)	705, 895
BfoI	RGCGCY	1 cut(s)	41
BfuAI	ACCTGC	1 cut(s)	809
BisI	GCNGC	7 cut(s)	119, 211, 258, 321, 414, 459, 753
BlsI	GCNGC	7 cut(s)	120, 212, 259, 322, 415, 460, 754
Bme1390I	CCNGG	6 cut(s)	51, 560, 725, 785, 814, 880
Bme18I	GGWCC	1 cut(s)	269
BmeRI	GACNNNNNGTC	1 cut(s)	156
BmeT110I	CYCGRG	1 cut(s)	630
BmgBI	CACGTC	1 cut(s)	153
BmgT120I	GGNCC	3 cut(s)	269, 698, 740
BmiI	GGNNCC	6 cut(s)	39, 238, 475, 540, 792, 823
BmrFI	CCNGG	6 cut(s)	51, 560, 725, 785, 814, 880
BmsI	GCATC	1 cut(s)	850
Bpu10I	CCTNAGC	2 cut(s)	206, 462
BpuEI	CTTGAG	1 cut(s)	593
BpuMI	CCSGG	3 cut(s)	51, 725, 814
BsaHI	GRCGYC	3 cut(s)	23, 38, 47
BsaJI	CCNNGG	3 cut(s)	431, 799, 813
BsaXI	ACNNNNNCTCC	3 cut(s)	522, 552, 874
Bsc4I	CCNNNNNNNGG	4 cut(s)	155, 707, 774, 800
Bse118I	RCCGGY	2 cut(s)	16, 40
Bse1I	ACTGG	1 cut(s)	559
BseBI	CCWGG	3 cut(s)	560, 785, 880
BseDI	CCNNGG	3 cut(s)	431, 799, 813
BseGI	GGATG	2 cut(s)	511, 865
BseLI	CCNNNNNNNGG	4 cut(s)	155, 707, 774, 800
BseMII	CTCAG	3 cut(s)	216, 220, 476
BseNI	ACTGG	1 cut(s)	559
BseSI	GKGCMC	1 cut(s)	382
BseXI	GCAGC	4 cut(s)	269, 332, 470, 739
Bsh1236I	CGCG	2 cut(s)	121, 407
BshFI	GGCC	5 cut(s)	366, 558, 700, 741, 878
BshNI	GGYRCC	3 cut(s)	37, 538, 821
BsiHKAI	GWGCWC	2 cut(s)	382, 554
BsiHKCI	CYCGRG	1 cut(s)	630
BsiSI	CCGG	6 cut(s)	17, 41, 50, 725, 756, 813
BslFI	GGGAC	1 cut(s)	428
BslI	CCNNNNNNNGG	4 cut(s)	155, 707, 774, 800
BsmAI	GTCTC	1 cut(s)	205
BsmFI	GGGAC	1 cut(s)	428
BsnI	GGCC	5 cut(s)	366, 558, 700, 741, 878
BsoBI	CYCGRG	1 cut(s)	630
Bsp1286I	GDGCHC	3 cut(s)	382, 476, 554
Bsp143I	GATC	1 cut(s)	309
Bsp19I	CCATGG	1 cut(s)	431
BspACI	CCGC	6 cut(s)	5, 119, 211, 299, 414, 528
BspANI	GGCC	5 cut(s)	366, 558, 700, 741, 878
BspCNI	CTCAG	4 cut(s)	4, 217, 219, 475
BspFNI	CGCG	2 cut(s)	121, 407
BspLI	GGNNCC	6 cut(s)	39, 238, 475, 540, 792, 823
BspMI	ACCTGC	1 cut(s)	809
BspT107I	GGYRCC	3 cut(s)	37, 538, 821
BsrBI	CCGCTC	1 cut(s)	299
BsrFI	RCCGGY	2 cut(s)	16, 40
BsrI	ACTGG	1 cut(s)	559
BssAI	RCCGGY	2 cut(s)	16, 40
BssECI	CCNNGG	3 cut(s)	431, 799, 813
BssMI	GATC	1 cut(s)	309
BssNI	GRCGYC	3 cut(s)	23, 38, 47
BssT1I	CCWWGG	2 cut(s)	431, 799
Bst2UI	CCWGG	3 cut(s)	560, 785, 880
Bst4CI	ACNGT	3 cut(s)	199, 538, 547
BstACI	GRCGYC	3 cut(s)	23, 38, 47
BstAPI	GCANNNNNTGC	1 cut(s)	263
BstC8I	GCNNGC	3 cut(s)	42, 134, 325
BstDEI	CTNAG	4 cut(s)	12, 206, 225, 462
BstDSI	CCRYGG	1 cut(s)	431
BstF5I	GGATG	2 cut(s)	511, 865
BstFNI	CGCG	2 cut(s)	121, 407
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	4 cut(s)	40, 329, 485, 873
BstKTI	GATC	1 cut(s)	312
BstMAI	GTCTC	1 cut(s)	205
BstMBI	GATC	1 cut(s)	309
BstNI	CCWGG	3 cut(s)	560, 785, 880
BstNSI	RCATGY	2 cut(s)	327, 523
BstSCI	CCNGG	6 cut(s)	49, 558, 723, 783, 812, 878
BstSLI	GKGCMC	1 cut(s)	382
BstUI	CGCG	2 cut(s)	121, 407
BstV1I	GCAGC	4 cut(s)	269, 332, 470, 739
BstXI	CCANNNNNNTGG	2 cut(s)	374, 566
BsuRI	GGCC	5 cut(s)	366, 558, 700, 741, 878
BtgI	CCRYGG	1 cut(s)	431
BtgZI	GCGATG	2 cut(s)	388, 439
BtrI	CACGTC	1 cut(s)	153
BtsCI	GGATG	2 cut(s)	511, 865
BtsI	GCAGTG	2 cut(s)	59, 609
BtsIMutI	CAGTG	3 cut(s)	59, 552, 609
BveI	ACCTGC	1 cut(s)	809
Cac8I	GCNNGC	3 cut(s)	42, 134, 325
CfoI	GCGC	4 cut(s)	40, 329, 485, 873
Cfr10I	RCCGGY	2 cut(s)	16, 40
Cfr13I	GGNCC	3 cut(s)	269, 698, 740
CseI	GACGC	2 cut(s)	31, 55
CsiI	ACCWGGT	1 cut(s)	783
Csp6I	GTAC	4 cut(s)	289, 517, 732, 822
CviAII	CATG	6 cut(s)	324, 432, 440, 520, 676, 874
CviQI	GTAC	4 cut(s)	289, 517, 732, 822
DdeI	CTNAG	4 cut(s)	12, 206, 225, 462
DinI	GGCGCC	1 cut(s)	39
DpnI	GATC	1 cut(s)	311
DpnII	GATC	1 cut(s)	309
DriI	GACNNNNNGTC	1 cut(s)	156
EaeI	YGGCCR	2 cut(s)	556, 876
Eam1105I	GACNNNNNGTC	1 cut(s)	156
EciI	GGCGGA	2 cut(s)	20, 543
Eco130I	CCWWGG	2 cut(s)	431, 799
Eco24I	GRGCYC	1 cut(s)	476
Eco47I	GGWCC	1 cut(s)	269
Eco88I	CYCGRG	1 cut(s)	630
EcoRII	CCWGG	3 cut(s)	558, 783, 878
EcoT14I	CCWWGG	2 cut(s)	431, 799
EcoT38I	GRGCYC	1 cut(s)	476
EgeI	GGCGCC	1 cut(s)	39
EheI	GGCGCC	1 cut(s)	39
ErhI	CCWWGG	2 cut(s)	431, 799
FaeI	CATG	6 cut(s)	327, 435, 443, 523, 679, 877
FaqI	GGGAC	1 cut(s)	428
FatI	CATG	6 cut(s)	323, 431, 439, 519, 675, 873
Fnu4HI	GCNGC	7 cut(s)	119, 211, 258, 321, 414, 459, 753
FokI	GGATG	2 cut(s)	498, 872
FriOI	GRGCYC	1 cut(s)	476
Fsp4HI	GCNGC	7 cut(s)	119, 211, 258, 321, 414, 459, 753
FspAI	RTGCGCAY	1 cut(s)	872
FspBI	CTAG	2 cut(s)	705, 895
FspI	TGCGCA	2 cut(s)	328, 872
GlaI	GCGC	4 cut(s)	39, 328, 484, 872
GluI	GCNGC	7 cut(s)	119, 211, 258, 321, 414, 459, 753
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	5 cut(s)	366, 558, 700, 741, 878
HapII	CCGG	6 cut(s)	17, 41, 50, 725, 756, 813
HgaI	GACGC	2 cut(s)	31, 55
HhaI	GCGC	4 cut(s)	40, 329, 485, 873
Hin1I	GRCGYC	3 cut(s)	23, 38, 47
Hin1II	CATG	6 cut(s)	327, 435, 443, 523, 679, 877
Hin6I	GCGC	4 cut(s)	38, 327, 483, 871
HinP1I	GCGC	4 cut(s)	38, 327, 483, 871
HindIII	AAGCTT	1 cut(s)	839
HinfI	GANTC	5 cut(s)	8, 89, 302, 352, 531
HpaII	CCGG	6 cut(s)	17, 41, 50, 725, 756, 813
HphI	GGTGA	2 cut(s)	31, 391
Hpy166II	GTNNAC	3 cut(s)	280, 380, 492
Hpy188I	TCNGA	4 cut(s)	13, 76, 150, 314
Hpy188III	TCNNGA	3 cut(s)	247, 277, 845
Hpy8I	GTNNAC	3 cut(s)	280, 380, 492
Hpy99I	CGWCG	3 cut(s)	49, 154, 894
HpyAV	CCTTC	1 cut(s)	250
HpyCH4III	ACNGT	3 cut(s)	199, 538, 547
HpyCH4IV	ACGT	1 cut(s)	152
HpyCH4V	TGCA	5 cut(s)	257, 320, 380, 620, 863
HpyF3I	CTNAG	4 cut(s)	12, 206, 225, 462
HpySE526I	ACGT	1 cut(s)	152
Hsp92I	GRCGYC	3 cut(s)	23, 38, 47
Hsp92II	CATG	6 cut(s)	327, 435, 443, 523, 679, 877
HspAI	GCGC	4 cut(s)	38, 327, 483, 871
KasI	GGCGCC	1 cut(s)	37
KpnI	GGTACC	1 cut(s)	825
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
Kzo9I	GATC	1 cut(s)	309
LmnI	GCTCC	7 cut(s)	151, 221, 296, 479, 796, 803, 882
Lsp1109I	GCAGC	4 cut(s)	269, 332, 470, 739
LweI	GCATC	1 cut(s)	850
MabI	ACCWGGT	1 cut(s)	783
MaeI	CTAG	2 cut(s)	705, 895
MaeII	ACGT	1 cut(s)	152
MaeIII	GTNAC	3 cut(s)	19, 532, 607
MalI	GATC	1 cut(s)	311
MbiI	CCGCTC	1 cut(s)	299
MboI	GATC	1 cut(s)	309
MboII	GAAGA	1 cut(s)	860
MhlI	GDGCHC	3 cut(s)	382, 476, 554
MlsI	TGGCCA	2 cut(s)	558, 878
MluNI	TGGCCA	2 cut(s)	558, 878
Mly113I	GGCGCC	1 cut(s)	38
MlyI	GAGTC	2 cut(s)	311, 540
MmeI	TCCRAC	2 cut(s)	173, 218
MnlI	CCTC	4 cut(s)	75, 215, 496, 847
Mox20I	TGGCCA	2 cut(s)	558, 878
MreI	CGCCGGCG	1 cut(s)	40
MroNI	GCCGGC	1 cut(s)	40
MscI	TGGCCA	2 cut(s)	558, 878
MseI	TTAA	1 cut(s)	591
MslI	CAYNNNNRTG	2 cut(s)	248, 689
Msp20I	TGGCCA	2 cut(s)	558, 878
MspA1I	CMGCKG	1 cut(s)	833
MspI	CCGG	6 cut(s)	17, 41, 50, 725, 756, 813
MspR9I	CCNGG	6 cut(s)	51, 560, 725, 785, 814, 880
MvaI	CCWGG	3 cut(s)	560, 785, 880
MvnI	CGCG	2 cut(s)	121, 407
NaeI	GCCGGC	1 cut(s)	42
NarI	GGCGCC	1 cut(s)	38
NciI	CCSGG	3 cut(s)	51, 725, 814
NcoI	CCATGG	1 cut(s)	431
NdeII	GATC	1 cut(s)	309
NgoMIV	GCCGGC	1 cut(s)	40
NlaIII	CATG	6 cut(s)	327, 435, 443, 523, 679, 877
NlaIV	GGNNCC	6 cut(s)	39, 238, 475, 540, 792, 823
NmeAIII	GCCGAG	2 cut(s)	105, 717
NmuCI	GTSAC	3 cut(s)	19, 532, 607
NsbI	TGCGCA	2 cut(s)	328, 872
NspI	RCATGY	2 cut(s)	327, 523
PaeI	GCATGC	1 cut(s)	327
PaeR7I	CTCGAG	1 cut(s)	630
PciI	ACATGT	1 cut(s)	519
PcsI	WCGNNNNNNNCGW	1 cut(s)	41
PdiI	GCCGGC	1 cut(s)	42
PfeI	GAWTC	3 cut(s)	8, 89, 352
PflMI	CCANNNNNTGG	2 cut(s)	707, 774
PkrI	GCNGC	7 cut(s)	120, 212, 259, 322, 415, 460, 754
PleI	GAGTC	2 cut(s)	310, 539
PluTI	GGCGCC	1 cut(s)	41
PpsI	GAGTC	2 cut(s)	310, 539
PscI	ACATGT	1 cut(s)	519
Psp6I	CCWGG	3 cut(s)	558, 783, 878
PspGI	CCWGG	3 cut(s)	558, 783, 878
PspN4I	GGNNCC	6 cut(s)	39, 238, 475, 540, 792, 823
PspPI	GGNCC	3 cut(s)	269, 698, 740
PspXI	VCTCGAGB	1 cut(s)	630
PsrI	GAACNNNNNNTAC	2 cut(s)	272, 304
PvuII	CAGCTG	1 cut(s)	833
RsaI	GTAC	4 cut(s)	290, 518, 733, 823
RsaNI	GTAC	4 cut(s)	289, 517, 732, 822
RseI	CAYNNNNRTG	2 cut(s)	248, 689
SaqAI	TTAA	1 cut(s)	591
SatI	GCNGC	7 cut(s)	119, 211, 258, 321, 414, 459, 753
Sau3AI	GATC	1 cut(s)	309
Sau96I	GGNCC	3 cut(s)	269, 698, 740
SchI	GAGTC	2 cut(s)	311, 540
ScrFI	CCNGG	6 cut(s)	51, 560, 725, 785, 814, 880
SduI	GDGCHC	3 cut(s)	382, 476, 554
SexAI	ACCWGGT	1 cut(s)	783
SfaNI	GCATC	1 cut(s)	850
SfoI	GGCGCC	1 cut(s)	39
Sfr274I	CTCGAG	1 cut(s)	630
SgrAI	CRCCGGYG	1 cut(s)	40
SinI	GGWCC	1 cut(s)	269
SlaI	CTCGAG	1 cut(s)	630
SmiMI	CAYNNNNRTG	2 cut(s)	248, 689
SmlI	CTYRAG	2 cut(s)	572, 630
SmoI	CTYRAG	2 cut(s)	572, 630
SphI	GCATGC	1 cut(s)	327
SsiI	CCGC	6 cut(s)	5, 119, 211, 299, 414, 528
SspDI	GGCGCC	1 cut(s)	37
SspMI	CTAG	2 cut(s)	705, 895
StyD4I	CCNGG	6 cut(s)	49, 558, 723, 783, 812, 878
StyI	CCWWGG	2 cut(s)	431, 799
TaaI	ACNGT	3 cut(s)	199, 538, 547
TaiI	ACGT	1 cut(s)	155
TaqI	TCGA	2 cut(s)	631, 844
TatI	WGTACW	1 cut(s)	288
TauI	GCSGC	3 cut(s)	121, 213, 416
TfiI	GAWTC	3 cut(s)	8, 89, 352
Tru1I	TTAA	1 cut(s)	591
Tru9I	TTAA	1 cut(s)	591
TscAI	CASTG	3 cut(s)	66, 559, 616
TseFI	GTSAC	3 cut(s)	19, 532, 607
TseI	GCWGC	4 cut(s)	257, 320, 458, 752
Tsp45I	GTSAC	3 cut(s)	19, 532, 607
TspDTI	ATGAA	4 cut(s)	232, 428, 568, 734
TspGWI	ACGGA	3 cut(s)	309, 504, 878
TspRI	CASTG	3 cut(s)	66, 559, 616
Van91I	CCANNNNNTGG	2 cut(s)	707, 774
VneI	GTGCAC	1 cut(s)	378
VpaK11BI	GGWCC	1 cut(s)	269
XceI	RCATGY	2 cut(s)	327, 523
XhoI	CTCGAG	1 cut(s)	630
XspI	CTAG	2 cut(s)	705, 895

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.