Rh2BG520000

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003423
Physical Location & Seq
Forward (+)
72977286 .. 72980659
3374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG520000.1

Sequence Viewer

Length: 507 bp
ATGAATCCGGTCCGGACTTTGGGGCCGGCAGTGGCTGCAGGACATTATAAGTCACTGTGGATCTACTTGGTTGCCCCAAGACTTGGTGCTTTAGCTGGTGCTGCTGCCCACACAGCTGCGAAGCTCTCAGAAATCCGACCAAACCAGCAGATCCATGATGCCAGTAATGATGAGACTACACCACAACCTCGCATTCATTTCCCCCCAGACAAATCTCCTTACCGGACAGGGGACTGGGACCGGCAGCTTTGGCCATGGCGGCGTCTCTTGCCCTTCAATGGCCGGCAGCTTGCTTGGTTGCTCCTAAACCCAAACGATGGCCTGCATTTCTTGTCAGAATCATCTGCTATAGCCAACGAAGCCAACTCCAAGGGTGATATTGTTCATTCCACCGGAGAATTCATTGCCGATCGTACCCTCCCTAAGAAGGCCATCAACCTCGACGCTACAGCGGCCTGTGTGCCGTCTAGCAGGTATAATGTGCAAGACACATGCACCAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

168

Amino Acids

18.52

Weight (kDa)

8.64

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 1 - 36 1.7e-07 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 462
AccB7I CCANNNNNTGG 2 cut(s) 83, 317
AccIII TCCGGA 1 cut(s) 12
AciI CCGC 2 cut(s) 259, 452
AclWI GGATC 2 cut(s) 68, 145
AcoI YGGCCR 2 cut(s) 251, 280
AcsI RAATTY 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 1 cut(s) 415
AfiI CCNNNNNNNGG 6 cut(s) 19, 83, 229, 278, 317, 427
AgsI TTSAA 1 cut(s) 277
AluBI AGCT 5 cut(s) 95, 116, 124, 247, 289
AluI AGCT 5 cut(s) 95, 116, 124, 247, 289
Alw26I GTCTC 2 cut(s) 167, 269
AlwI GGATC 2 cut(s) 68, 145
AlwNI CAGNNNCTG 1 cut(s) 35
Aor13HI TCCGGA 1 cut(s) 12
AoxI GGCC 6 cut(s) 23, 251, 280, 319, 429, 453
ApeKI GCWGC 6 cut(s) 35, 101, 104, 116, 244, 286
ApoI RAATTY 1 cut(s) 398
AspS9I GGNCC 3 cut(s) 10, 23, 238
AsuHPI GGTGA 1 cut(s) 386
AvaII GGWCC 2 cut(s) 10, 238
BalI TGGCCA 1 cut(s) 253
BbvI GCAGC 6 cut(s) 22, 88, 91, 103, 256, 298
BccI CCATC 2 cut(s) 311, 440
BceAI ACGGC 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 167, 269
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 3 cut(s) 36, 348, 447
BfuAI ACCTGC 1 cut(s) 462
BglI GCCNNNNNGGC 1 cut(s) 259
BisI GCNGC 8 cut(s) 36, 102, 105, 117, 245, 260, 287, 453
BlsI GCNGC 8 cut(s) 37, 103, 106, 118, 246, 261, 288, 454
Bme18I GGWCC 2 cut(s) 10, 238
BmgT120I GGNCC 3 cut(s) 10, 23, 238
BmiI GGNNCC 2 cut(s) 24, 239
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 254, 369
BsaWI WCCGGW 4 cut(s) 7, 12, 222, 392
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 83, 229, 278, 317, 427
Bse118I RCCGGY 3 cut(s) 25, 240, 282
Bse1I ACTGG 2 cut(s) 162, 239
Bse3DI GCAATG 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 254, 369
BseLI CCNNNNNNNGG 6 cut(s) 19, 83, 229, 278, 317, 427
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 2 cut(s) 162, 239
BseXI GCAGC 6 cut(s) 22, 88, 91, 103, 256, 298
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 6 cut(s) 25, 253, 282, 321, 431, 455
BsiEI CGRYCG 1 cut(s) 412
BsiSI CCGG 7 cut(s) 8, 13, 26, 223, 241, 283, 393
BslFI GGGAC 2 cut(s) 245, 251
BslI CCNNNNNNNGG 6 cut(s) 19, 83, 229, 278, 317, 427
BsmAI GTCTC 2 cut(s) 167, 269
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 245, 251
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 6 cut(s) 25, 253, 282, 321, 431, 455
Bsp13I TCCGGA 1 cut(s) 12
Bsp143I GATC 3 cut(s) 60, 150, 409
Bsp19I CCATGG 1 cut(s) 254
BspACI CCGC 2 cut(s) 259, 452
BspANI GGCC 6 cut(s) 25, 253, 282, 321, 431, 455
BspCNI CTCAG 1 cut(s) 140
BspEI TCCGGA 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 24, 239
BspMAI CTGCAG 1 cut(s) 40
BspMI ACCTGC 1 cut(s) 462
BspPI GGATC 2 cut(s) 68, 145
BsrDI GCAATG 1 cut(s) 402
BsrFI RCCGGY 3 cut(s) 25, 240, 282
BsrI ACTGG 2 cut(s) 162, 239
BssAI RCCGGY 3 cut(s) 25, 240, 282
BssECI CCNNGG 2 cut(s) 254, 369
BssMI GATC 3 cut(s) 60, 150, 409
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 2 cut(s) 254, 369
Bst4CI ACNGT 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 262
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 4 cut(s) 27, 284, 291, 323
BstDEI CTNAG 2 cut(s) 127, 423
BstDSI CCRYGG 1 cut(s) 254
BstKTI GATC 3 cut(s) 63, 153, 412
BstMAI GTCTC 2 cut(s) 167, 269
BstMBI GATC 3 cut(s) 60, 150, 409
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 8 cut(s) 35, 101, 113, 250, 259, 268, 359, 452
BstNSI RCATGY 1 cut(s) 495
BstSFI CTRYAG 3 cut(s) 36, 348, 447
BstV1I GCAGC 6 cut(s) 22, 88, 91, 103, 256, 298
BstX2I RGATCY 2 cut(s) 60, 150
BstYI RGATCY 2 cut(s) 60, 150
BsuRI GGCC 6 cut(s) 25, 253, 282, 321, 431, 455
BtgI CCRYGG 1 cut(s) 254
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 2 cut(s) 36, 53
BveI ACCTGC 1 cut(s) 462
Cac8I GCNNGC 4 cut(s) 27, 284, 291, 323
CaiI CAGNNNCTG 1 cut(s) 35
Cfr10I RCCGGY 3 cut(s) 25, 240, 282
Cfr13I GGNCC 3 cut(s) 10, 23, 238
CpoI CGGWCCG 1 cut(s) 10
CseI GACGC 2 cut(s) 251, 452
Csp6I GTAC 1 cut(s) 414
CspI CGGWCCG 1 cut(s) 10
CviAII CATG 3 cut(s) 155, 255, 492
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 2 cut(s) 127, 423
DpnI GATC 3 cut(s) 62, 152, 411
DpnII GATC 3 cut(s) 60, 150, 409
EaeI YGGCCR 2 cut(s) 251, 280
Eco130I CCWWGG 2 cut(s) 254, 369
Eco47I GGWCC 2 cut(s) 10, 238
EcoRI GAATTC 1 cut(s) 398
EcoT14I CCWWGG 2 cut(s) 254, 369
ErhI CCWWGG 2 cut(s) 254, 369
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 3 cut(s) 158, 258, 495
FaiI YATR 6 cut(s) 48, 156, 256, 350, 477, 493
FaqI GGGAC 2 cut(s) 245, 251
FatI CATG 3 cut(s) 154, 254, 491
Fnu4HI GCNGC 8 cut(s) 36, 102, 105, 117, 245, 260, 287, 453
Fsp4HI GCNGC 8 cut(s) 36, 102, 105, 117, 245, 260, 287, 453
FspBI CTAG 1 cut(s) 468
GluI GCNGC 8 cut(s) 36, 102, 105, 117, 245, 260, 287, 453
HaeIII GGCC 6 cut(s) 25, 253, 282, 321, 431, 455
HapII CCGG 7 cut(s) 8, 13, 26, 223, 241, 283, 393
HgaI GACGC 2 cut(s) 251, 452
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 3 cut(s) 158, 258, 495
HinfI GANTC 2 cut(s) 4, 338
HpaII CCGG 7 cut(s) 8, 13, 26, 223, 241, 283, 393
HphI GGTGA 1 cut(s) 386
Hpy188I TCNGA 3 cut(s) 130, 137, 337
Hpy188III TCNNGA 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 2 cut(s) 283, 421
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4V TGCA 4 cut(s) 38, 325, 484, 495
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 101, 113, 250, 259, 268, 359, 452
HpyF3I CTNAG 2 cut(s) 127, 423
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 3 cut(s) 158, 258, 495
Kpn2I TCCGGA 1 cut(s) 12
KroI GCCGGC 2 cut(s) 25, 282
KroNI GCCGGC 2 cut(s) 27, 284
Kzo9I GATC 3 cut(s) 60, 150, 409
LmnI GCTCC 1 cut(s) 306
Lsp1109I GCAGC 6 cut(s) 22, 88, 91, 103, 256, 298
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 51
MalI GATC 3 cut(s) 62, 152, 411
MboI GATC 3 cut(s) 60, 150, 409
MflI RGATCY 2 cut(s) 60, 150
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 1 cut(s) 398
MluNI TGGCCA 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 3 cut(s) 198, 428, 449
Mox20I TGGCCA 1 cut(s) 253
MroI TCCGGA 1 cut(s) 12
MroNI GCCGGC 2 cut(s) 25, 282
MscI TGGCCA 1 cut(s) 253
Msp20I TGGCCA 1 cut(s) 253
MspA1I CMGCKG 2 cut(s) 116, 452
MspI CCGG 7 cut(s) 8, 13, 26, 223, 241, 283, 393
Mva1269I GAATGC 1 cut(s) 192
MwoI GCNNNNNNNGC 8 cut(s) 35, 101, 113, 250, 259, 268, 359, 452
NaeI GCCGGC 2 cut(s) 27, 284
NcoI CCATGG 1 cut(s) 254
NdeII GATC 3 cut(s) 60, 150, 409
NgoMIV GCCGGC 2 cut(s) 25, 282
NlaIII CATG 3 cut(s) 158, 258, 495
NlaIV GGNNCC 2 cut(s) 24, 239
NmuCI GTSAC 1 cut(s) 51
NspI RCATGY 1 cut(s) 495
PctI GAATGC 1 cut(s) 192
PdiI GCCGGC 2 cut(s) 27, 284
PfeI GAWTC 2 cut(s) 4, 338
PflMI CCANNNNNTGG 2 cut(s) 83, 317
PkrI GCNGC 8 cut(s) 37, 103, 106, 118, 246, 261, 288, 454
Ple19I CGATCG 1 cut(s) 412
PsiI TTATAA 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 24, 239
PspPI GGNCC 3 cut(s) 10, 23, 238
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 60, 150
PvuI CGATCG 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
Rsr2I CGGWCCG 1 cut(s) 10
RsrII CGGWCCG 1 cut(s) 10
SatI GCNGC 8 cut(s) 36, 102, 105, 117, 245, 260, 287, 453
Sau3AI GATC 3 cut(s) 60, 150, 409
Sau96I GGNCC 3 cut(s) 10, 23, 238
SetI ASST 8 cut(s) 97, 118, 126, 190, 249, 291, 441, 476
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 3 cut(s) 36, 348, 447
SinI GGWCC 2 cut(s) 10, 238
Sse9I AATT 1 cut(s) 398
SsiI CCGC 2 cut(s) 259, 452
SspMI CTAG 1 cut(s) 468
StyI CCWWGG 2 cut(s) 254, 369
TaaI ACNGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 441
TasI AATT 1 cut(s) 398
TauI GCSGC 2 cut(s) 262, 455
TfiI GAWTC 2 cut(s) 4, 338
TscAI CASTG 2 cut(s) 36, 60
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 6 cut(s) 35, 101, 104, 116, 244, 286
Tsp45I GTSAC 1 cut(s) 51
TspDTI ATGAA 4 cut(s) 17, 185, 374, 391
TspRI CASTG 2 cut(s) 36, 60
Van91I CCANNNNNTGG 2 cut(s) 83, 317
VpaK11BI GGWCC 2 cut(s) 10, 238
XapI RAATTY 1 cut(s) 398
XceI RCATGY 1 cut(s) 495
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.