RchiOBHm_Chr2g0154411

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003423
Physical Location & Seq
Forward (+)
71603735 .. 71604279
545 bp
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UTR
Exon/CDS
Intron
PRQ52340

Sequence Viewer

Length: 309 bp
ATGCCAGGAAGCAGAAGCTTGAAGAGCTTCCTTGTGCTCGATGCCACTTTTATCCGTAAGGTTGGAGCGGAGTTTGTTGGGACCTTCATTATGATATTTGCGGCAGCAGCAACAGCAGCACCTATCGTGAACCAAAAGTACGATGGCGCGGAGACACTGCTAGGCAACGCAACATGTGCAAGGCTAGCAGTGATGGTAGTGGTTTTGTCCATCGGCCACATCTCCGGAGCACACCTAAACCCATCAGTCACTATCGCATTTGCAGCGTTTCAACACTTCCCATGGTCCCAAGTTCCCCTCTACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

102

Amino Acids

10.84

Weight (kDa)

9.03

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 15 - 102 4.6e-19 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 68
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 3 cut(s) 68, 101, 149
AcoI YGGCCR 1 cut(s) 214
AfaI GTAC 1 cut(s) 140
AflIII ACRYGT 1 cut(s) 173
AgsI TTSAA 2 cut(s) 22, 272
AjnI CCWGG 1 cut(s) 4
AluBI AGCT 2 cut(s) 18, 27
AluI AGCT 2 cut(s) 18, 27
Alw21I GWGCWC 2 cut(s) 39, 232
Alw26I GTCTC 1 cut(s) 146
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 4 cut(s) 104, 107, 116, 263
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 81, 285
AsuNHI GCTAGC 1 cut(s) 184
AvaII GGWCC 2 cut(s) 81, 285
Bbv12I GWGCWC 2 cut(s) 39, 232
BbvI GCAGC 4 cut(s) 116, 119, 128, 275
BccI CCATC 4 cut(s) 137, 187, 218, 250
BciT130I CCWGG 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 2 cut(s) 161, 185
BisI GCNGC 5 cut(s) 102, 105, 108, 117, 264
BlsI GCNGC 5 cut(s) 103, 106, 109, 118, 265
Bme1390I CCNGG 1 cut(s) 6
Bme18I GGWCC 2 cut(s) 81, 285
BmgT120I GGNCC 2 cut(s) 81, 285
BmiI GGNNCC 2 cut(s) 82, 287
BmrFI CCNGG 1 cut(s) 6
BmsI GCATC 1 cut(s) 31
BmtI GCTAGC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 224
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 281
BseXI GCAGC 4 cut(s) 116, 119, 128, 275
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 39, 232
BsiSI CCGG 1 cut(s) 225
BslFI GGGAC 2 cut(s) 94, 271
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 94, 271
BsnI GGCC 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 39, 232
Bsp13I TCCGGA 1 cut(s) 224
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 3 cut(s) 68, 101, 149
BspANI GGCC 1 cut(s) 216
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 82, 287
BspOI GCTAGC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 6
Bst6I CTCTTC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 186
BstDSI CCRYGG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 149
BstMAI GTCTC 1 cut(s) 146
BstMWI GCNNNNNNNGC 7 cut(s) 24, 107, 113, 116, 176, 185, 263
BstNI CCWGG 1 cut(s) 6
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 4
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 4 cut(s) 116, 119, 128, 275
BsuRI GGCC 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 281
BtsI GCAGTG 2 cut(s) 155, 195
BtsIMutI CAGTG 2 cut(s) 155, 195
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 81, 285
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 174, 282
CviJI RGCY 4 cut(s) 18, 27, 184, 216
CviKI_1 RGCY 4 cut(s) 18, 27, 184, 216
CviQI GTAC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 281
Eco47I GGWCC 2 cut(s) 81, 285
EcoO109I RGGNCCY 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 2 cut(s) 177, 285
FaiI YATR 5 cut(s) 92, 175, 283, 305, 307
FaqI GGGAC 2 cut(s) 94, 271
FatI CATG 2 cut(s) 173, 281
Fnu4HI GCNGC 5 cut(s) 102, 105, 108, 117, 264
Fsp4HI GCNGC 5 cut(s) 102, 105, 108, 117, 264
FspBI CTAG 2 cut(s) 161, 185
GlaI GCGC 1 cut(s) 148
GluI GCNGC 5 cut(s) 102, 105, 108, 117, 264
HaeIII GGCC 1 cut(s) 216
HapII CCGG 1 cut(s) 225
HhaI GCGC 1 cut(s) 149
Hin1II CATG 2 cut(s) 177, 285
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 16
HpaII CCGG 1 cut(s) 225
Hpy166II GTNNAC 1 cut(s) 130
Hpy188III TCNNGA 2 cut(s) 127, 225
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 94
HpyCH4V TGCA 2 cut(s) 179, 263
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 107, 113, 116, 176, 185, 263
Hsp92II CATG 2 cut(s) 177, 285
HspAI GCGC 1 cut(s) 147
Kpn2I TCCGGA 1 cut(s) 224
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 2 cut(s) 65, 227
LpnPI CCDG 2 cut(s) 18, 238
Lsp1109I GCAGC 4 cut(s) 116, 119, 128, 275
LweI GCATC 1 cut(s) 31
MaeI CTAG 2 cut(s) 161, 185
MaeIII GTNAC 1 cut(s) 247
MbiI CCGCTC 1 cut(s) 68
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 2 cut(s) 39, 232
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 1 cut(s) 308
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 26
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 1 cut(s) 6
MvaI CCWGG 1 cut(s) 6
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 7 cut(s) 24, 107, 113, 116, 176, 185, 263
NcoI CCATGG 1 cut(s) 281
NheI GCTAGC 1 cut(s) 184
NlaIII CATG 2 cut(s) 177, 285
NlaIV GGNNCC 2 cut(s) 82, 287
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 177
PciI ACATGT 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 26
PkrI GCNGC 5 cut(s) 103, 106, 109, 118, 265
PpuMI RGGWCCY 1 cut(s) 81
PscI ACATGT 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
PspN4I GGNNCC 2 cut(s) 82, 287
PspPI GGNCC 2 cut(s) 81, 285
PspPPI RGGWCCY 1 cut(s) 81
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SapI GCTCTTC 1 cut(s) 17
SatI GCNGC 5 cut(s) 102, 105, 108, 117, 264
Sau96I GGNCC 2 cut(s) 81, 285
ScrFI CCNGG 1 cut(s) 6
SduI GDGCHC 2 cut(s) 39, 232
SetI ASST 6 cut(s) 20, 29, 63, 86, 124, 237
SfaNI GCATC 1 cut(s) 31
SinI GGWCC 2 cut(s) 81, 285
SsiI CCGC 3 cut(s) 68, 101, 149
SspMI CTAG 2 cut(s) 161, 185
StyD4I CCNGG 1 cut(s) 4
StyI CCWWGG 1 cut(s) 281
TaqI TCGA 1 cut(s) 39
TauI GCSGC 1 cut(s) 104
TscAI CASTG 2 cut(s) 162, 195
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 4 cut(s) 104, 107, 116, 263
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 76
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 2 cut(s) 162, 195
VpaK11BI GGWCC 2 cut(s) 81, 285
XceI RCATGY 1 cut(s) 177
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 2 cut(s) 161, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.