Rh2DG530300

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003423
Physical Location & Seq
Forward (+)
75901015 .. 75901352
338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG530300.1

Sequence Viewer

Length: 234 bp
ATGAATCCGGTCCGGACTTTGGGGCCGGCAGTGGCTGCAGGACATTATAAGTCACTGTGGATCTACTTGGTTGCCCCAAGACTTGGTGCTTTAGCTGGTGCTGCTGCCCACACAGCTGCGAAGCTCTCAGAAATCCGACCAAACCAGCAGATCCATGATGCCAGTAATGATGAGACTACACCACAACCTCGCATTCATTTCCCCCCAGACAAATCTCCTTACCGGACAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

77

Amino Acids

8.31

Weight (kDa)

9.69

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 1 - 36 3.5e-08 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 12
AclWI GGATC 2 cut(s) 68, 145
AfiI CCNNNNNNNGG 2 cut(s) 19, 83
AluBI AGCT 3 cut(s) 95, 116, 124
AluI AGCT 3 cut(s) 95, 116, 124
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 2 cut(s) 68, 145
AlwNI CAGNNNCTG 1 cut(s) 35
Aor13HI TCCGGA 1 cut(s) 12
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 4 cut(s) 35, 101, 104, 116
AspS9I GGNCC 2 cut(s) 10, 23
AvaII GGWCC 1 cut(s) 10
BbvI GCAGC 4 cut(s) 22, 88, 91, 103
BcoDI GTCTC 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 4 cut(s) 36, 102, 105, 117
BlsI GCNGC 4 cut(s) 37, 103, 106, 118
Bme18I GGWCC 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 10, 23
BmiI GGNNCC 1 cut(s) 24
BmsI GCATC 1 cut(s) 148
BsaWI WCCGGW 3 cut(s) 7, 12, 222
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 83
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 162
BseAI TCCGGA 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 19, 83
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 162
BseXI GCAGC 4 cut(s) 22, 88, 91, 103
BshFI GGCC 1 cut(s) 25
BsiSI CCGG 4 cut(s) 8, 13, 26, 223
BslI CCNNNNNNNGG 2 cut(s) 19, 83
BsmAI GTCTC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 1 cut(s) 25
Bsp13I TCCGGA 1 cut(s) 12
Bsp143I GATC 2 cut(s) 60, 150
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 140
BspEI TCCGGA 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 24
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 2 cut(s) 68, 145
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 162
BssAI RCCGGY 1 cut(s) 25
BssMI GATC 2 cut(s) 60, 150
Bst4CI ACNGT 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 1 cut(s) 127
BstKTI GATC 2 cut(s) 63, 153
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 2 cut(s) 60, 150
BstMWI GCNNNNNNNGC 3 cut(s) 35, 101, 113
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 4 cut(s) 22, 88, 91, 103
BstX2I RGATCY 2 cut(s) 60, 150
BstYI RGATCY 2 cut(s) 60, 150
BsuRI GGCC 1 cut(s) 25
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 2 cut(s) 36, 53
Cac8I GCNNGC 1 cut(s) 27
CaiI CAGNNNCTG 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 10, 23
CpoI CGGWCCG 1 cut(s) 10
CspI CGGWCCG 1 cut(s) 10
CviAII CATG 1 cut(s) 155
CviJI RGCY 5 cut(s) 25, 35, 95, 116, 124
CviKI_1 RGCY 5 cut(s) 25, 35, 95, 116, 124
DdeI CTNAG 1 cut(s) 127
DpnI GATC 2 cut(s) 62, 152
DpnII GATC 2 cut(s) 60, 150
Eco47I GGWCC 1 cut(s) 10
FaeI CATG 1 cut(s) 158
FaiI YATR 2 cut(s) 48, 156
FatI CATG 1 cut(s) 154
Fnu4HI GCNGC 4 cut(s) 36, 102, 105, 117
Fsp4HI GCNGC 4 cut(s) 36, 102, 105, 117
GluI GCNGC 4 cut(s) 36, 102, 105, 117
HaeIII GGCC 1 cut(s) 25
HapII CCGG 4 cut(s) 8, 13, 26, 223
Hin1II CATG 1 cut(s) 158
HinfI GANTC 1 cut(s) 4
HpaII CCGG 4 cut(s) 8, 13, 26, 223
Hpy188I TCNGA 2 cut(s) 130, 137
Hpy188III TCNNGA 1 cut(s) 13
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4V TGCA 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 101, 113
HpyF3I CTNAG 1 cut(s) 127
Hsp92II CATG 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 12
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 2 cut(s) 60, 150
LpnPI CCDG 9 cut(s) 21, 24, 26, 39, 81, 158, 175, 213, 219
Lsp1109I GCAGC 4 cut(s) 22, 88, 91, 103
LweI GCATC 1 cut(s) 148
MaeIII GTNAC 1 cut(s) 51
MalI GATC 2 cut(s) 62, 152
MboI GATC 2 cut(s) 60, 150
MflI RGATCY 2 cut(s) 60, 150
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 1 cut(s) 198
MroI TCCGGA 1 cut(s) 12
MroNI GCCGGC 1 cut(s) 25
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 4 cut(s) 8, 13, 26, 223
Mva1269I GAATGC 1 cut(s) 192
MwoI GCNNNNNNNGC 3 cut(s) 35, 101, 113
NaeI GCCGGC 1 cut(s) 27
NdeII GATC 2 cut(s) 60, 150
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 51
PctI GAATGC 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 27
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 83
PkrI GCNGC 4 cut(s) 37, 103, 106, 118
PsiI TTATAA 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 2 cut(s) 10, 23
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 60, 150
PvuII CAGCTG 1 cut(s) 116
Rsr2I CGGWCCG 1 cut(s) 10
RsrII CGGWCCG 1 cut(s) 10
SatI GCNGC 4 cut(s) 36, 102, 105, 117
Sau3AI GATC 2 cut(s) 60, 150
Sau96I GGNCC 2 cut(s) 10, 23
SetI ASST 4 cut(s) 97, 118, 126, 190
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 1 cut(s) 10
TaaI ACNGT 1 cut(s) 57
TfiI GAWTC 1 cut(s) 4
TscAI CASTG 2 cut(s) 36, 60
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 4 cut(s) 35, 101, 104, 116
Tsp45I GTSAC 1 cut(s) 51
TspDTI ATGAA 2 cut(s) 17, 185
TspRI CASTG 2 cut(s) 36, 60
Van91I CCANNNNNTGG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.