RLG00000020776

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003423
Physical Location & Seq
Forward (+)
68621940 .. 68622983
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020776

Sequence Viewer

Length: 705 bp
ATGTCAAGAGAACGCTGGATCTGTCTGGAAGTCTTCTGCCACGTCATACAACCAACAGTTGGGACCTTCATTATGATATTTGCGGCAGCAGCAGCACCTATCGTGAACCAAAAGTACGATGGCGCAGAGACGCTGCAGGGCAACGCAACATGTGCAAGGCTAGCAGTGATGCGTTTCAACACTTCCCCCCAAGTTCCCCTCTACATAATAGCGCAGGTTCTGGCGTCCATCTCTGCAAGTCTTGCTCTCAAGGGTGTTTTTCATCCATTTATGAACGGTGGAGTTACAGTTCCTTCAGTAGGCAATGGCCAGGCTTTTGCACTCGAGTTCATTGTCACTTTCAACCTCATGTTTGTCATCATTTCTGTTGCCACAGATACCCGTGCGGTAGGGGAGTTGGCAGGTTTAGCAATTGGAGCTACAGTATTGCTCGACATTCTTATAGCAGGTGTTGTAACAGGCGCTTCAATGAATCCGGTCCGAACTTTGGGGCCTGCAGTGGCTGCAGGACATTATAAGTCACTGTGGATCTACTTGGTTGCCCCAGCACTTGGTGCTTTAGCTGATGCTGCTGCCTACACAGCTGTGAAGCTCTCAGAAATCCGACCAAACCAGCAGATCCATGATGCCAGTATTAATGGTGAGACTACACCACAACCTCGCATTCATTTCCCCTCAGACAAATCTCCTTACCGGACAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

235

Amino Acids

24.74

Weight (kDa)

7.77

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 55 - 193 1.6e-31 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AarI CACCTGC 1 cut(s) 437
Acc36I ACCTGC 3 cut(s) 205, 392, 437
AccB7I CCANNNNNTGG 2 cut(s) 59, 551
AciI CCGC 2 cut(s) 83, 386
AclWI GGATC 3 cut(s) 26, 536, 613
AcoI YGGCCR 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 224
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 4 cut(s) 59, 299, 487, 551
AflIII ACRYGT 1 cut(s) 149
AgsI TTSAA 3 cut(s) 178, 343, 468
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 1 cut(s) 309
AleI CACNNNNGTG 1 cut(s) 584
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
AluBI AGCT 4 cut(s) 419, 563, 584, 592
AluI AGCT 4 cut(s) 419, 563, 584, 592
Alw26I GTCTC 2 cut(s) 122, 638
AlwI GGATC 3 cut(s) 26, 536, 613
AlwNI CAGNNNCTG 2 cut(s) 220, 503
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 2 cut(s) 307, 491
ApeKI GCWGC 7 cut(s) 86, 89, 92, 133, 503, 569, 572
AseI ATTAAT 1 cut(s) 636
AspLEI GCGC 3 cut(s) 125, 214, 464
AspS9I GGNCC 3 cut(s) 63, 478, 491
AsuHPI GGTGA 1 cut(s) 653
AsuNHI GCTAGC 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 323
AvaII GGWCC 2 cut(s) 63, 478
BalI TGGCCA 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 25
BbvI GCAGC 7 cut(s) 98, 101, 104, 120, 490, 556, 559
BccI CCATC 2 cut(s) 113, 236
BciT130I CCWGG 1 cut(s) 311
BcoDI GTCTC 2 cut(s) 122, 638
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 4 cut(s) 134, 420, 495, 504
BfoI RGCGCY 1 cut(s) 465
BfuAI ACCTGC 3 cut(s) 205, 392, 437
BisI GCNGC 8 cut(s) 84, 87, 90, 93, 134, 504, 570, 573
BlsI GCNGC 8 cut(s) 85, 88, 91, 94, 135, 505, 571, 574
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 2 cut(s) 63, 478
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 3 cut(s) 63, 478, 491
BmiI GGNNCC 2 cut(s) 64, 492
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 3 cut(s) 159, 556, 616
BmtI GCTAGC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 233
BsaHI GRCGYC 1 cut(s) 224
BsaWI WCCGGW 2 cut(s) 475, 693
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 408, 438
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 299, 487, 551
Bse1I ACTGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 311
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 4 cut(s) 59, 299, 487, 551
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 2 cut(s) 609, 690
BseNI ACTGG 1 cut(s) 630
BseXI GCAGC 7 cut(s) 98, 101, 104, 120, 490, 556, 559
BseYI CCCAGC 1 cut(s) 544
BshFI GGCC 2 cut(s) 309, 493
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 2 cut(s) 476, 694
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 4 cut(s) 59, 299, 487, 551
BsmAI GTCTC 2 cut(s) 122, 638
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 2 cut(s) 309, 493
BsoBI CYCGRG 1 cut(s) 323
Bsp143I GATC 3 cut(s) 18, 528, 618
BspACI CCGC 2 cut(s) 83, 386
BspANI GGCC 2 cut(s) 309, 493
BspCNI CTCAG 2 cut(s) 608, 689
BspLI GGNNCC 2 cut(s) 64, 492
BspMAI CTGCAG 3 cut(s) 138, 499, 508
BspMI ACCTGC 3 cut(s) 205, 392, 437
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 3 cut(s) 26, 536, 613
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 1 cut(s) 630
BssMI GATC 3 cut(s) 18, 528, 618
BssNI GRCGYC 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 5 cut(s) 58, 278, 289, 424, 525
BstACI GRCGYC 1 cut(s) 224
BstAPI GCANNNNNTGC 4 cut(s) 152, 242, 503, 554
BstC8I GCNNGC 2 cut(s) 162, 495
BstDEI CTNAG 2 cut(s) 595, 676
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 3 cut(s) 125, 214, 464
BstKTI GATC 3 cut(s) 21, 531, 621
BstMAI GTCTC 2 cut(s) 122, 638
BstMBI GATC 3 cut(s) 18, 528, 618
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 4 cut(s) 134, 420, 495, 504
BstV1I GCAGC 7 cut(s) 98, 101, 104, 120, 490, 556, 559
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 3 cut(s) 18, 528, 618
BstYI RGATCY 3 cut(s) 18, 528, 618
BsuRI GGCC 2 cut(s) 309, 493
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 1 cut(s) 262
BtsI GCAGTG 2 cut(s) 171, 504
BtsIMutI CAGTG 3 cut(s) 171, 504, 521
BveI ACCTGC 3 cut(s) 205, 392, 437
Cac8I GCNNGC 2 cut(s) 162, 495
CaiI CAGNNNCTG 2 cut(s) 220, 503
CfoI GCGC 3 cut(s) 125, 214, 464
Cfr13I GGNCC 3 cut(s) 63, 478, 491
CpoI CGGWCCG 1 cut(s) 478
CseI GACGC 2 cut(s) 139, 213
Csp6I GTAC 1 cut(s) 115
CspI CGGWCCG 1 cut(s) 478
CviAII CATG 3 cut(s) 150, 349, 623
CviJI RGCY 9 cut(s) 160, 309, 314, 419, 493, 503, 563, 584, 592
CviKI_1 RGCY 9 cut(s) 160, 309, 314, 419, 493, 503, 563, 584, 592
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 595, 676
DpnI GATC 3 cut(s) 20, 530, 620
DpnII GATC 3 cut(s) 18, 528, 618
DraIII CACNNNGTG 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 63, 478
Eco57I CTGAAG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 323
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 2 cut(s) 63, 491
EcoRII CCWGG 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 3 cut(s) 153, 352, 626
FaiI YATR 9 cut(s) 47, 74, 151, 206, 272, 350, 443, 516, 624
FaqI GGGAC 1 cut(s) 76
FatI CATG 3 cut(s) 149, 348, 622
Fnu4HI GCNGC 8 cut(s) 84, 87, 90, 93, 134, 504, 570, 573
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 8 cut(s) 84, 87, 90, 93, 134, 504, 570, 573
FspBI CTAG 1 cut(s) 161
GlaI GCGC 3 cut(s) 124, 213, 463
GluI GCNGC 8 cut(s) 84, 87, 90, 93, 134, 504, 570, 573
GsaI CCCAGC 1 cut(s) 548
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 2 cut(s) 309, 493
HapII CCGG 2 cut(s) 476, 694
HgaI GACGC 2 cut(s) 139, 213
HhaI GCGC 3 cut(s) 125, 214, 464
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 3 cut(s) 153, 352, 626
Hin6I GCGC 3 cut(s) 123, 212, 462
HinP1I GCGC 3 cut(s) 123, 212, 462
HinfI GANTC 1 cut(s) 472
HpaII CCGG 2 cut(s) 476, 694
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 482, 598, 605, 679
Hpy188III TCNNGA 3 cut(s) 6, 26, 103
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 2 cut(s) 76, 303
HpyCH4III ACNGT 5 cut(s) 58, 278, 289, 424, 525
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 6 cut(s) 136, 155, 236, 320, 497, 506
HpyF3I CTNAG 2 cut(s) 595, 676
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 3 cut(s) 153, 352, 626
HspAI GCGC 3 cut(s) 123, 212, 462
Kzo9I GATC 3 cut(s) 18, 528, 618
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 7 cut(s) 98, 101, 104, 120, 490, 556, 559
LweI GCATC 3 cut(s) 159, 556, 616
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 283, 334, 454, 519
MalI GATC 3 cut(s) 20, 530, 620
MboI GATC 3 cut(s) 18, 528, 618
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 3 cut(s) 18, 528, 618
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 1 cut(s) 411
MluNI TGGCCA 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 4 cut(s) 209, 356, 669, 685
Mox20I TGGCCA 1 cut(s) 309
MscI TGGCCA 1 cut(s) 309
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 476, 694
MspR9I CCNGG 1 cut(s) 311
MunI CAATTG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 663
MvaI CCWGG 1 cut(s) 311
NdeII GATC 3 cut(s) 18, 528, 618
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 3 cut(s) 153, 352, 626
NlaIV GGNNCC 2 cut(s) 64, 492
NmuCI GTSAC 2 cut(s) 334, 519
NspI RCATGY 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 323
PaqCI CACCTGC 1 cut(s) 437
PciI ACATGT 1 cut(s) 149
PctI GAATGC 1 cut(s) 663
PfeI GAWTC 1 cut(s) 472
PflMI CCANNNNNTGG 2 cut(s) 59, 551
PkrI GCNGC 8 cut(s) 85, 88, 91, 94, 135, 505, 571, 574
PpuMI RGGWCCY 1 cut(s) 63
PscI ACATGT 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 636
PsiI TTATAA 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 309
PspFI CCCAGC 1 cut(s) 544
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 64, 492
PspPI GGNCC 3 cut(s) 63, 478, 491
PspPPI RGGWCCY 1 cut(s) 63
PspXI VCTCGAGB 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 98, 130
PstI CTGCAG 3 cut(s) 138, 499, 508
PstNI CAGNNNCTG 2 cut(s) 220, 503
PsuI RGATCY 3 cut(s) 18, 528, 618
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 584
Rsr2I CGGWCCG 1 cut(s) 478
RsrII CGGWCCG 1 cut(s) 478
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 8 cut(s) 84, 87, 90, 93, 134, 504, 570, 573
Sau3AI GATC 3 cut(s) 18, 528, 618
Sau96I GGNCC 3 cut(s) 63, 478, 491
ScrFI CCNGG 1 cut(s) 311
SfaNI GCATC 3 cut(s) 159, 556, 616
SfcI CTRYAG 4 cut(s) 134, 420, 495, 504
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 2 cut(s) 63, 478
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 248, 323
SmoI CTYRAG 2 cut(s) 248, 323
Sse9I AATT 1 cut(s) 411
SsiI CCGC 2 cut(s) 83, 386
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 309
TaaI ACNGT 5 cut(s) 58, 278, 289, 424, 525
TaiI ACGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 324, 432
TasI AATT 1 cut(s) 411
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 472
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 3 cut(s) 171, 504, 528
TseFI GTSAC 2 cut(s) 334, 519
TseI GCWGC 7 cut(s) 86, 89, 92, 133, 503, 569, 572
Tsp45I GTSAC 2 cut(s) 334, 519
TspDTI ATGAA 6 cut(s) 58, 251, 287, 319, 485, 656
TspRI CASTG 3 cut(s) 171, 504, 528
Van91I CCANNNNNTGG 2 cut(s) 59, 551
VpaK11BI GGWCC 2 cut(s) 63, 478
VspI ATTAAT 1 cut(s) 636
XagI CCTNNNNNAGG 1 cut(s) 297
XceI RCATGY 1 cut(s) 153
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XhoI CTCGAG 1 cut(s) 323
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.