Rroxscaffold_2G00094350

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003423
Physical Location & Seq
Reverse (-)
15650844 .. 15651380
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00094350.1

Sequence Viewer

Length: 327 bp
ATGGCCAAGCTTTTGCACTCGATCGAGGTCATTGTCACTTTCAACCTCATGTTTGTCATCATTTCTGTTGCCACAGATACCCGTGCGGTAGGGGAGTTGGCAGGTTTGGGAGTTGGAGCTACAGTATTGCTCGACATTCTTGTAGCAGGGCAAACAACCGGCGCTTCAATGAATCCGGTCCGGACTTTGGGGCCGGCAGTGGCTGCAGGACATTATAAGTCACTGTGGATGTACTTGGTTGCCCCAACACTTGGTGCTTTAATTAGCTGGTGCTGCTGCCCACACAGCTGTGAAGCTCTCAGAAATCCGACCAAACCAGCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

108

Amino Acids

11.27

Weight (kDa)

7.71

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 8 - 89 2.3e-21 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 251
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 3
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 187, 251
AgsI TTSAA 2 cut(s) 43, 168
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 5 cut(s) 10, 119, 267, 288, 296
AluI AGCT 5 cut(s) 10, 119, 267, 288, 296
AlwNI CAGNNNCTG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 180
AoxI GGCC 2 cut(s) 3, 191
ApeKI GCWGC 3 cut(s) 203, 273, 276
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 178, 191
AvaII GGWCC 1 cut(s) 178
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 3 cut(s) 190, 260, 263
BfmI CTRYAG 2 cut(s) 120, 204
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 92
BisI GCNGC 3 cut(s) 204, 274, 277
BlsI GCNGC 3 cut(s) 205, 275, 278
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 2 cut(s) 178, 191
BmiI GGNNCC 1 cut(s) 192
BsaWI WCCGGW 2 cut(s) 175, 180
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 251
Bse118I RCCGGY 2 cut(s) 158, 193
BseAI TCCGGA 1 cut(s) 180
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 187, 251
BseMII CTCAG 1 cut(s) 313
BseXI GCAGC 3 cut(s) 190, 260, 263
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 5, 193
BsiEI CGRYCG 1 cut(s) 24
BsiSI CCGG 4 cut(s) 159, 176, 181, 194
BslI CCNNNNNNNGG 2 cut(s) 187, 251
BsnI GGCC 2 cut(s) 5, 193
Bsp13I TCCGGA 1 cut(s) 180
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 1 cut(s) 86
BspANI GGCC 2 cut(s) 5, 193
BspCNI CTCAG 1 cut(s) 312
BspEI TCCGGA 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 192
BspMAI CTGCAG 1 cut(s) 208
BspMI ACCTGC 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 158, 193
BssAI RCCGGY 2 cut(s) 158, 193
BssMI GATC 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 124, 225
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 234
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 3 cut(s) 203, 273, 285
BstSFI CTRYAG 2 cut(s) 120, 204
BstV1I GCAGC 3 cut(s) 190, 260, 263
BsuRI GGCC 2 cut(s) 5, 193
BtsCI GGATG 1 cut(s) 234
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 221
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 195
CaiI CAGNNNCTG 1 cut(s) 203
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 2 cut(s) 158, 193
Cfr13I GGNCC 2 cut(s) 178, 191
CpoI CGGWCCG 1 cut(s) 178
Csp6I GTAC 1 cut(s) 232
CspI CGGWCCG 1 cut(s) 178
CviAII CATG 1 cut(s) 49
CviJI RGCY 8 cut(s) 5, 10, 119, 193, 203, 267, 288, 296
CviKI_1 RGCY 8 cut(s) 5, 10, 119, 193, 203, 267, 288, 296
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 1 cut(s) 299
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 178
FaeI CATG 1 cut(s) 52
FaiI YATR 2 cut(s) 50, 216
FatI CATG 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 204, 274, 277
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 3 cut(s) 204, 274, 277
GlaI GCGC 1 cut(s) 163
GluI GCNGC 3 cut(s) 204, 274, 277
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 5, 193
HapII CCGG 4 cut(s) 159, 176, 181, 194
HhaI GCGC 1 cut(s) 164
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 1 cut(s) 172
HpaII CCGG 4 cut(s) 159, 176, 181, 194
Hpy188I TCNGA 2 cut(s) 302, 309
Hpy188III TCNNGA 1 cut(s) 181
HpyCH4III ACNGT 2 cut(s) 124, 225
HpyCH4V TGCA 2 cut(s) 16, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 273, 285
HpyF3I CTNAG 1 cut(s) 299
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 180
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 8 cut(s) 87, 132, 172, 189, 192, 194, 207, 253
Lsp1109I GCAGC 3 cut(s) 190, 260, 263
MaeIII GTNAC 2 cut(s) 34, 219
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 261
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 2 cut(s) 19, 56
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 180
MroNI GCCGGC 1 cut(s) 193
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 4 cut(s) 159, 176, 181, 194
MwoI GCNNNNNNNGC 3 cut(s) 203, 273, 285
NaeI GCCGGC 1 cut(s) 195
NdeII GATC 1 cut(s) 21
NgoMIV GCCGGC 1 cut(s) 193
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 34, 219
OliI CACNNNNGTG 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 172
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 3 cut(s) 205, 275, 278
Ple19I CGATCG 1 cut(s) 24
PsiI TTATAA 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 2 cut(s) 178, 191
PstI CTGCAG 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 203
PvuI CGATCG 1 cut(s) 24
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 288
Rsr2I CGGWCCG 1 cut(s) 178
RsrII CGGWCCG 1 cut(s) 178
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 3 cut(s) 204, 274, 277
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 2 cut(s) 178, 191
SetI ASST 8 cut(s) 12, 30, 48, 106, 121, 269, 290, 298
SfcI CTRYAG 2 cut(s) 120, 204
SinI GGWCC 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 288
Sse9I AATT 1 cut(s) 261
SsiI CCGC 1 cut(s) 86
TaaI ACNGT 2 cut(s) 124, 225
TaqI TCGA 3 cut(s) 20, 24, 132
TasI AATT 1 cut(s) 261
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 1 cut(s) 172
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TscAI CASTG 2 cut(s) 204, 228
TseFI GTSAC 2 cut(s) 34, 219
TseI GCWGC 3 cut(s) 203, 273, 276
Tsp45I GTSAC 2 cut(s) 34, 219
TspDTI ATGAA 1 cut(s) 185
TspRI CASTG 2 cut(s) 204, 228
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.