FvH4_6g41330

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004058
Physical Location & Seq
Reverse (-)
32521128 .. 32523704
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41330.t1

Sequence Viewer

Length: 1089 bp
ATGGCCAATTTGACGATGAAGGGTCTTCTCAAGAAATCCAAGTCAAGCAAGCCGTCCGAGCTCCTCAAGAACACTCGTGAGCTTCTCATCTTCCTCAATGCCGATAAGGACGTCCGCGAGCAGAAGTGCAAGGAGAAGATGGAAGAATTAACAAAATCAATGTTGGAGATTAGAACACTTCTGTATGGGAATGGTGAATCAGAGCCGAATAAAGACACTTGTGCACAGCTTACTCAGGAATTTTTCAGAGAGGATATGTTCCGCCTTCTCATCGCTAGCCTTTCAAAGCTGAACCTAGAGACTCGTAAAAACGCAACGCATATTATTGCAAATCTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCCGAATATTTGGAGAAAAATATTGATATTATGGATCTTCTGATACCTGGTTACGAAGAAGGTGATATTGCTCTGTCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCAAGGTACGTCCTGGAATCAAACCACATGAAAAAGTTCTTTGATTATATACAAATTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACCTTCAAGGAGCTTATGACCAGGCATAAATCCACTGTTGCACAATTTCTGTCAAATAATTATGTCTGGTTTTTCCAAGAATATAATTCGCAGTTACTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTGTCAAACTGCTAGGAAATATGTTACTGGATCGCTCAAACTCCGATGTGATGGTTCGATACGTTTGCTCTTTGGATAACATGAGAATCCTCATGAACCTTCTCAGGGATCCAAACAAGACGATCCAATTAGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAACGAAAATAAGCCTCCTGAGATTGCTAATGTACTAGTAACAAATAGGAGCAAGCTTCTCCGTTTCTTTGGCGACTTCAACATTGAGAAAGAGGATATGCAGTTTGAAGCAGACAAAGCTGAACTCATTAAAGAAATCTCTATTCTTGAACCCAAGGACATTAAGGACTTCTCTTCAGTCCTGAGCTTTAGAGAGCATTGTCAGATTGCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

363

Amino Acids

42.17

Weight (kDa)

7.02

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 10 - 340 5.5e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
AccII CGCG 2 cut(s) 117, 358
AciI CCGC 2 cut(s) 115, 262
AclWI GGATC 5 cut(s) 411, 747, 812, 825, 826
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 239, 543, 550
AcuI CTGAAG 1 cut(s) 1032
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 2 cut(s) 497, 906
AfiI CCNNNNNNNGG 1 cut(s) 814
AgsI TTSAA 8 cut(s) 285, 353, 557, 586, 859, 952, 980, 1022
AhlI ACTAGT 1 cut(s) 907
AjnI CCWGG 3 cut(s) 415, 501, 599
AluBI AGCT 9 cut(s) 61, 82, 229, 289, 592, 862, 928, 992, 1059
AluI AGCT 9 cut(s) 61, 82, 229, 289, 592, 862, 928, 992, 1059
Alw21I GWGCWC 2 cut(s) 63, 226
Alw26I GTCTC 3 cut(s) 293, 687, 836
Alw44I GTGCAC 1 cut(s) 222
AlwI GGATC 5 cut(s) 411, 747, 812, 825, 826
AlwNI CAGNNNCTG 1 cut(s) 676
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 222
ApeKI GCWGC 2 cut(s) 572, 575
ApoI RAATTY 3 cut(s) 239, 543, 550
Asp700I GAANNNNTTC 2 cut(s) 471, 524
AsuHPI GGTGA 3 cut(s) 206, 443, 688
AsuNHI GCTAGC 1 cut(s) 275
BaeGI GKGCMC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 817
BanII GRGCYC 1 cut(s) 63
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 2 cut(s) 17, 847
Bbv12I GWGCWC 2 cut(s) 63, 226
BbvI GCAGC 2 cut(s) 559, 587
BccI CCATC 2 cut(s) 133, 754
BceAI ACGGC 2 cut(s) 37, 719
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 3 cut(s) 417, 503, 601
BcoDI GTCTC 3 cut(s) 293, 687, 836
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 4 cut(s) 276, 296, 722, 908
BfmI CTRYAG 1 cut(s) 573
BisI GCNGC 2 cut(s) 573, 576
BlsI GCNGC 2 cut(s) 574, 577
Bme1390I CCNGG 3 cut(s) 417, 503, 601
BmiI GGNNCC 1 cut(s) 819
BmrFI CCNGG 3 cut(s) 417, 503, 601
BmsI GCATC 2 cut(s) 559, 572
BmtI GCTAGC 1 cut(s) 279
BpiI GAAGAC 2 cut(s) 17, 847
BpmI CTGGAG 1 cut(s) 698
Bpu10I CCTNAGC 1 cut(s) 1055
BpuEI CTTGAG 2 cut(s) 14, 50
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 1 cut(s) 1026
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse1I ACTGG 2 cut(s) 681, 741
BseBI CCWGG 3 cut(s) 417, 503, 601
BseDI CCNNGG 1 cut(s) 1026
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMII CTCAG 5 cut(s) 248, 698, 826, 882, 1046
BseNI ACTGG 2 cut(s) 681, 741
BseRI GAGGAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 226
BseXI GCAGC 2 cut(s) 559, 587
Bsh1236I CGCG 2 cut(s) 117, 358
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 63, 226
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 3 cut(s) 293, 687, 836
BsmI GAATGC 2 cut(s) 471, 473
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 63, 226
Bsp143I GATC 4 cut(s) 403, 739, 817, 831
Bsp68I TCGCGA 1 cut(s) 358
BspACI CCGC 2 cut(s) 115, 262
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 247, 697, 825, 883, 1047
BspFNI CGCG 2 cut(s) 117, 358
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 1 cut(s) 819
BspMAI CTGCAG 1 cut(s) 577
BspOI GCTAGC 1 cut(s) 279
BspPI GGATC 5 cut(s) 411, 747, 812, 825, 826
BspTNI GGTCTC 1 cut(s) 836
BsrI ACTGG 2 cut(s) 681, 741
BssECI CCNNGG 1 cut(s) 1026
BssMI GATC 4 cut(s) 403, 739, 817, 831
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 1026
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 417, 503, 601
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 1 cut(s) 1051
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 569
BstC8I GCNNGC 5 cut(s) 50, 119, 277, 708, 926
BstDEI CTNAG 5 cut(s) 234, 684, 812, 891, 1055
BstF5I GGATG 1 cut(s) 574
BstFNI CGCG 2 cut(s) 117, 358
BstKTI GATC 4 cut(s) 406, 742, 820, 834
BstMAI GTCTC 3 cut(s) 293, 687, 836
BstMBI GATC 4 cut(s) 403, 739, 817, 831
BstMWI GCNNNNNNNGC 3 cut(s) 58, 569, 989
BstNI CCWGG 3 cut(s) 417, 503, 601
BstNSI RCATGY 1 cut(s) 1086
BstSCI CCNGG 3 cut(s) 415, 501, 599
BstSFI CTRYAG 1 cut(s) 573
BstSLI GKGCMC 1 cut(s) 226
BstUI CGCG 2 cut(s) 117, 358
BstV1I GCAGC 2 cut(s) 559, 587
BstV2I GAAGAC 2 cut(s) 17, 847
BstX2I RGATCY 2 cut(s) 403, 817
BstYI RGATCY 2 cut(s) 403, 817
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 574
BtsIMutI CAGTG 1 cut(s) 612
BtuMI TCGCGA 1 cut(s) 358
Cac8I GCNNGC 5 cut(s) 50, 119, 277, 708, 926
CaiI CAGNNNCTG 1 cut(s) 676
CciI TCATGA 1 cut(s) 801
CsiI ACCWGGT 1 cut(s) 415
Csp6I GTAC 2 cut(s) 496, 905
CviAII CATG 5 cut(s) 517, 790, 802, 851, 1083
CviQI GTAC 2 cut(s) 496, 905
DdeI CTNAG 5 cut(s) 234, 684, 812, 891, 1055
DpnI GATC 4 cut(s) 405, 741, 819, 833
DpnII GATC 4 cut(s) 403, 739, 817, 831
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1051
EarI CTCTTC 1 cut(s) 1051
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 1026
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 836
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 1032
EcoICRI GAGCTC 1 cut(s) 61
EcoRII CCWGG 3 cut(s) 415, 501, 599
EcoT14I CCWWGG 1 cut(s) 1026
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 1026
FaeI CATG 5 cut(s) 520, 793, 805, 854, 1086
FatI CATG 5 cut(s) 516, 789, 801, 850, 1082
Fnu4HI GCNGC 2 cut(s) 573, 576
FokI GGATG 1 cut(s) 581
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 2 cut(s) 573, 576
FspBI CTAG 4 cut(s) 276, 296, 722, 908
GluI GCNGC 2 cut(s) 573, 576
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 1 cut(s) 5
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 5 cut(s) 520, 793, 805, 854, 1086
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 5 cut(s) 197, 301, 506, 680, 795
HphI GGTGA 3 cut(s) 206, 443, 688
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 9 cut(s) 58, 202, 248, 373, 411, 568, 687, 754, 1077
Hpy188III TCNNGA 9 cut(s) 31, 67, 77, 236, 357, 802, 890, 1019, 1054
Hpy8I GTNNAC 1 cut(s) 224
HpyAV CCTTC 5 cut(s) 13, 275, 422, 592, 818
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 3 cut(s) 111, 498, 771
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 569, 989
HpyF3I CTNAG 5 cut(s) 234, 684, 812, 891, 1055
HpySE526I ACGT 3 cut(s) 111, 498, 771
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 5 cut(s) 520, 793, 805, 854, 1086
Kzo9I GATC 4 cut(s) 403, 739, 817, 831
LmnI GCTCC 3 cut(s) 66, 589, 921
Lsp1109I GCAGC 2 cut(s) 559, 587
LweI GCATC 2 cut(s) 559, 572
MabI ACCWGGT 1 cut(s) 415
MaeI CTAG 4 cut(s) 276, 296, 722, 908
MaeII ACGT 3 cut(s) 111, 498, 771
MaeIII GTNAC 4 cut(s) 419, 672, 732, 910
MalI GATC 4 cut(s) 405, 741, 819, 833
MboI GATC 4 cut(s) 403, 739, 817, 831
MboII GAAGA 8 cut(s) 17, 82, 148, 155, 398, 437, 847, 1038
MflI RGATCY 2 cut(s) 403, 817
MhlI GDGCHC 2 cut(s) 63, 226
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 295, 689
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 74, 104, 244, 809, 897, 958
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 473
MroXI GAANNNNTTC 2 cut(s) 471, 524
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 149, 461, 1002, 1035, 1087
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 3 cut(s) 417, 503, 601
Mva1269I GAATGC 2 cut(s) 471, 473
MvaI CCWGG 3 cut(s) 417, 503, 601
MvnI CGCG 2 cut(s) 117, 358
MwoI GCNNNNNNNGC 3 cut(s) 58, 569, 989
NdeII GATC 4 cut(s) 403, 739, 817, 831
NheI GCTAGC 1 cut(s) 275
NlaIII CATG 5 cut(s) 520, 793, 805, 854, 1086
NlaIV GGNNCC 1 cut(s) 819
NruI TCGCGA 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 1 cut(s) 1086
PagI TCATGA 1 cut(s) 801
PctI GAATGC 2 cut(s) 471, 473
PdmI GAANNNNTTC 2 cut(s) 471, 524
PfeI GAWTC 3 cut(s) 197, 506, 795
PfoI TCCNGGA 1 cut(s) 501
PkrI GCNGC 2 cut(s) 574, 577
PleI GAGTC 2 cut(s) 295, 688
PpsI GAGTC 2 cut(s) 295, 688
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 3 cut(s) 415, 501, 599
PspGI CCWGG 3 cut(s) 415, 501, 599
PspN4I GGNNCC 1 cut(s) 819
PstI CTGCAG 1 cut(s) 577
PstNI CAGNNNCTG 1 cut(s) 676
PsuI RGATCY 2 cut(s) 403, 817
RruI TCGCGA 1 cut(s) 358
RsaI GTAC 2 cut(s) 497, 906
RsaNI GTAC 2 cut(s) 496, 905
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 5 cut(s) 149, 461, 1002, 1035, 1087
SatI GCNGC 2 cut(s) 573, 576
Sau3AI GATC 4 cut(s) 403, 739, 817, 831
SchI GAGTC 2 cut(s) 295, 689
ScrFI CCNGG 3 cut(s) 417, 503, 601
SduI GDGCHC 2 cut(s) 63, 226
SexAI ACCWGGT 1 cut(s) 415
SfaNI GCATC 2 cut(s) 559, 572
SfcI CTRYAG 1 cut(s) 573
SmlI CTYRAG 2 cut(s) 29, 65
SmoI CTYRAG 2 cut(s) 29, 65
SpeI ACTAGT 1 cut(s) 907
SsiI CCGC 2 cut(s) 115, 262
SspI AATATT 2 cut(s) 377, 391
SspMI CTAG 4 cut(s) 276, 296, 722, 908
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 3 cut(s) 415, 501, 599
StyI CCWWGG 1 cut(s) 1026
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 3 cut(s) 114, 501, 774
TaqI TCGA 1 cut(s) 766
TatI WGTACW 1 cut(s) 904
TfiI GAWTC 3 cut(s) 197, 506, 795
Tru1I TTAA 5 cut(s) 149, 461, 1002, 1035, 1087
Tru9I TTAA 5 cut(s) 149, 461, 1002, 1035, 1087
TscAI CASTG 1 cut(s) 619
TseI GCWGC 2 cut(s) 572, 575
TspDTI ATGAA 4 cut(s) 32, 533, 818, 839
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 1 cut(s) 619
VneI GTGCAC 1 cut(s) 222
XapI RAATTY 3 cut(s) 239, 543, 550
XceI RCATGY 1 cut(s) 1086
XmnI GAANNNNTTC 2 cut(s) 471, 524
XspI CTAG 4 cut(s) 276, 296, 722, 908
ZraI GACGTC 1 cut(s) 112
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.