Rw2G043150

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004058
Physical Location & Seq
Reverse (-)
69494430 .. 69497114
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043150.1

Sequence Viewer

Length: 1074 bp
ATGAAGGGTCTTCTCAAGAAATCCAAGGCACGCAAGCCTTTGGAGCTCGTCAAGCACACGCGCGAGCTTCTCATCTTCTTCAATGGCAACATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGATGGAAGAATTAACAAAATCAATGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCGAATAAAGACACTTGTGCACAGCTTACCCAGGAATTTTTCAAAGATGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGAGACTCGTAAAAACGCAACACATATTGTCGCAAATTTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTACTTGGAAAAGAATATTGATCTTATAGATATTCTGATACCTGGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGGACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTGGATCGCTCAAACTCTGCTGTGATGGTTCGATACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGATCCAAACAAGACAATCCAATTGGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAATGAAAATAAGCCTCCTCAGATTGTTAATGTACTAGTAACAAATAGGAGCAAGCTTCTCCGTTTCTTTGGCAACTTCACCATTGAAAAAGAGGATCAGCAGTTTGAAGCAGACAAAGTTGAACTTATGAAAGAGATTTCTATTCTTGAACCCAAGGATTCTAAGGAATTTTCGTCGATCGTGAGCTTTAGAGAGAATTGTGAGATTCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

357

Amino Acids

41.63

Weight (kDa)

8.13

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 5 - 335 1.6e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 61, 63, 102, 343
AciI CCGC 2 cut(s) 100, 247
AclWI GGATC 4 cut(s) 732, 797, 810, 960
AcsI RAATTY 4 cut(s) 224, 316, 535, 1025
AfaI GTAC 3 cut(s) 362, 482, 891
AfiI CCNNNNNNNGG 2 cut(s) 407, 799
AflIII ACRYGT 1 cut(s) 756
AhlI ACTAGT 1 cut(s) 892
AjnI CCWGG 4 cut(s) 219, 400, 486, 584
AluBI AGCT 9 cut(s) 46, 67, 214, 274, 577, 703, 847, 913, 1044
AluI AGCT 9 cut(s) 46, 67, 214, 274, 577, 703, 847, 913, 1044
Alw21I GWGCWC 3 cut(s) 48, 211, 764
Alw26I GTCTC 4 cut(s) 278, 405, 672, 821
Alw44I GTGCAC 1 cut(s) 207
AlwI GGATC 4 cut(s) 732, 797, 810, 960
AlwNI CAGNNNCTG 1 cut(s) 661
ApaLI GTGCAC 1 cut(s) 207
ApeKI GCWGC 2 cut(s) 557, 560
ApoI RAATTY 4 cut(s) 224, 316, 535, 1025
Asp700I GAANNNNTTC 2 cut(s) 456, 509
AspLEI GCGC 1 cut(s) 63
AsuHPI GGTGA 4 cut(s) 191, 428, 673, 928
AsuII TTCGAA 1 cut(s) 269
AsuNHI GCTAGC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 211
BamHI GGATCC 1 cut(s) 802
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 2 cut(s) 2, 832
Bbv12I GWGCWC 3 cut(s) 48, 211, 764
BbvI GCAGC 2 cut(s) 544, 572
BccI CCATC 3 cut(s) 118, 170, 739
BceAI ACGGC 1 cut(s) 704
BcgI CGANNNNNNTGC 2 cut(s) 741, 775
BciT130I CCWGG 4 cut(s) 221, 402, 488, 586
BcoDI GTCTC 4 cut(s) 278, 405, 672, 821
BcuI ACTAGT 1 cut(s) 892
BfaI CTAG 2 cut(s) 261, 893
BfmI CTRYAG 1 cut(s) 558
BisI GCNGC 2 cut(s) 558, 561
BlsI GCNGC 2 cut(s) 559, 562
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 4 cut(s) 221, 402, 488, 586
BmiI GGNNCC 1 cut(s) 804
BmrFI CCNGG 4 cut(s) 221, 402, 488, 586
BmsI GCATC 2 cut(s) 544, 557
BmtI GCTAGC 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 2, 832
BpmI CTGGAG 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 757
BsaI GGTCTC 1 cut(s) 821
BsaJI CCNNGG 3 cut(s) 24, 219, 1011
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 799
Bse1I ACTGG 2 cut(s) 635, 726
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 4 cut(s) 221, 402, 488, 586
BseDI CCNNGG 3 cut(s) 24, 219, 1011
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 2 cut(s) 407, 799
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 4 cut(s) 348, 683, 811, 890
BseNI ACTGG 2 cut(s) 635, 726
BseRI GAGGAG 1 cut(s) 864
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 2 cut(s) 544, 572
Bsh1236I CGCG 4 cut(s) 61, 63, 102, 343
Bsh1285I CGRYCG 1 cut(s) 1038
BsiEI CGRYCG 1 cut(s) 1038
BsiHKAI GWGCWC 3 cut(s) 48, 211, 764
BslI CCNNNNNNNGG 2 cut(s) 407, 799
BsmAI GTCTC 4 cut(s) 278, 405, 672, 821
BsmBI CGTCTC 1 cut(s) 405
BsmI GAATGC 2 cut(s) 456, 458
Bso31I GGTCTC 1 cut(s) 821
Bsp119I TTCGAA 1 cut(s) 269
Bsp1286I GDGCHC 3 cut(s) 48, 211, 764
Bsp143I GATC 5 cut(s) 379, 724, 802, 952, 1035
Bsp68I TCGCGA 1 cut(s) 343
BspACI CCGC 2 cut(s) 100, 247
BspCNI CTCAG 4 cut(s) 349, 682, 810, 889
BspFNI CGCG 4 cut(s) 61, 63, 102, 343
BspLI GGNNCC 1 cut(s) 804
BspMAI CTGCAG 1 cut(s) 562
BspOI GCTAGC 1 cut(s) 264
BspPI GGATC 4 cut(s) 732, 797, 810, 960
BspT104I TTCGAA 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 821
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 2 cut(s) 635, 726
BssECI CCNNGG 3 cut(s) 24, 219, 1011
BssMI GATC 5 cut(s) 379, 724, 802, 952, 1035
BssT1I CCWWGG 2 cut(s) 24, 1011
Bst2UI CCWGG 4 cut(s) 221, 402, 488, 586
Bst4CI ACNGT 2 cut(s) 416, 601
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 757
BstBI TTCGAA 1 cut(s) 269
BstC8I GCNNGC 7 cut(s) 31, 35, 65, 104, 262, 693, 911
BstDEI CTNAG 5 cut(s) 357, 669, 797, 876, 1020
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 4 cut(s) 61, 63, 102, 343
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 5 cut(s) 382, 727, 805, 955, 1038
BstMAI GTCTC 4 cut(s) 278, 405, 672, 821
BstMBI GATC 5 cut(s) 379, 724, 802, 952, 1035
BstMCI CGRYCG 1 cut(s) 1038
BstMWI GCNNNNNNNGC 3 cut(s) 43, 52, 554
BstNI CCWGG 4 cut(s) 221, 402, 488, 586
BstSCI CCNGG 4 cut(s) 219, 400, 486, 584
BstSFI CTRYAG 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 4 cut(s) 61, 63, 102, 343
BstV1I GCAGC 2 cut(s) 544, 572
BstV2I GAAGAC 2 cut(s) 2, 832
BstX2I RGATCY 1 cut(s) 802
BstYI RGATCY 1 cut(s) 802
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 1 cut(s) 559
BtuMI TCGCGA 1 cut(s) 343
Cac8I GCNNGC 7 cut(s) 31, 35, 65, 104, 262, 693, 911
CaiI CAGNNNCTG 1 cut(s) 661
CfoI GCGC 1 cut(s) 63
CsiI ACCWGGT 1 cut(s) 400
Csp6I GTAC 3 cut(s) 361, 481, 890
CviAII CATG 5 cut(s) 91, 502, 775, 836, 1068
CviQI GTAC 3 cut(s) 361, 481, 890
DdeI CTNAG 5 cut(s) 357, 669, 797, 876, 1020
DpnI GATC 5 cut(s) 381, 726, 804, 954, 1037
DpnII GATC 5 cut(s) 379, 724, 802, 952, 1035
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 24, 1011
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 821
Eco53kI GAGCTC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 46
EcoRII CCWGG 4 cut(s) 219, 400, 486, 584
EcoT14I CCWWGG 2 cut(s) 24, 1011
EcoT22I ATGCAT 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 24, 1011
Esp3I CGTCTC 1 cut(s) 405
FaeI CATG 5 cut(s) 94, 505, 778, 839, 1071
FalI AAGNNNNNCTT 4 cut(s) 497, 529, 966, 998
FatI CATG 5 cut(s) 90, 501, 774, 835, 1067
Fnu4HI GCNGC 2 cut(s) 558, 561
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 2 cut(s) 558, 561
FspBI CTAG 2 cut(s) 261, 893
GlaI GCGC 1 cut(s) 62
GluI GCNGC 2 cut(s) 558, 561
GsuI CTGGAG 1 cut(s) 683
HhaI GCGC 1 cut(s) 63
Hin1II CATG 5 cut(s) 94, 505, 778, 839, 1071
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 7 cut(s) 182, 286, 491, 665, 780, 1016, 1063
HphI GGTGA 4 cut(s) 191, 428, 673, 928
Hpy166II GTNNAC 2 cut(s) 209, 597
Hpy188I TCNGA 7 cut(s) 187, 358, 396, 496, 553, 672, 879
Hpy188III TCNNGA 4 cut(s) 16, 342, 1004, 1039
Hpy8I GTNNAC 2 cut(s) 209, 597
Hpy99I CGWCG 1 cut(s) 1036
HpyAV CCTTC 2 cut(s) 260, 803
HpyCH4III ACNGT 2 cut(s) 416, 601
HpyCH4IV ACGT 3 cut(s) 242, 483, 756
HpyCH4V TGCA 6 cut(s) 209, 322, 440, 456, 560, 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 52, 554
HpyF3I CTNAG 5 cut(s) 357, 669, 797, 876, 1020
HpySE526I ACGT 3 cut(s) 242, 483, 756
Hsp92II CATG 5 cut(s) 94, 505, 778, 839, 1071
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 379, 724, 802, 952, 1035
LmnI GCTCC 3 cut(s) 43, 574, 906
Lsp1109I GCAGC 2 cut(s) 544, 572
LweI GCATC 2 cut(s) 544, 557
MabI ACCWGGT 1 cut(s) 400
MaeI CTAG 2 cut(s) 261, 893
MaeII ACGT 3 cut(s) 242, 483, 756
MaeIII GTNAC 2 cut(s) 717, 895
MalI GATC 5 cut(s) 381, 726, 804, 954, 1037
MboI GATC 5 cut(s) 379, 724, 802, 952, 1035
MboII GAAGA 5 cut(s) 67, 70, 140, 517, 832
MfeI CAATTG 1 cut(s) 821
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 3 cut(s) 48, 211, 764
MlyI GAGTC 2 cut(s) 280, 674
MmeI TCCRAC 2 cut(s) 129, 583
MnlI CCTC 4 cut(s) 471, 882, 885, 943
Mph1103I ATGCAT 1 cut(s) 458
MroXI GAANNNNTTC 2 cut(s) 456, 509
MseI TTAA 3 cut(s) 134, 446, 885
MspR9I CCNGG 4 cut(s) 221, 402, 488, 586
MunI CAATTG 1 cut(s) 821
Mva1269I GAATGC 2 cut(s) 456, 458
MvaI CCWGG 4 cut(s) 221, 402, 488, 586
MvnI CGCG 4 cut(s) 61, 63, 102, 343
MwoI GCNNNNNNNGC 3 cut(s) 43, 52, 554
NdeII GATC 5 cut(s) 379, 724, 802, 952, 1035
NheI GCTAGC 1 cut(s) 260
NlaIII CATG 5 cut(s) 94, 505, 778, 839, 1071
NlaIV GGNNCC 1 cut(s) 804
NruI TCGCGA 1 cut(s) 343
NsiI ATGCAT 1 cut(s) 458
NspV TTCGAA 1 cut(s) 269
PctI GAATGC 2 cut(s) 456, 458
PdmI GAANNNNTTC 2 cut(s) 456, 509
PfeI GAWTC 5 cut(s) 182, 491, 780, 1016, 1063
PfoI TCCNGGA 1 cut(s) 486
PkrI GCNGC 2 cut(s) 559, 562
Ple19I CGATCG 1 cut(s) 1038
PleI GAGTC 2 cut(s) 280, 673
PpsI GAGTC 2 cut(s) 280, 673
Ppu21I YACGTR 1 cut(s) 757
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 4 cut(s) 219, 400, 486, 584
PspGI CCWGG 4 cut(s) 219, 400, 486, 584
PspN4I GGNNCC 1 cut(s) 804
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 661
PsuI RGATCY 1 cut(s) 802
PvuI CGATCG 1 cut(s) 1038
RruI TCGCGA 1 cut(s) 343
RsaI GTAC 3 cut(s) 362, 482, 891
RsaNI GTAC 3 cut(s) 361, 481, 890
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 134, 446, 885
SatI GCNGC 2 cut(s) 558, 561
Sau3AI GATC 5 cut(s) 379, 724, 802, 952, 1035
ScaI AGTACT 1 cut(s) 362
SchI GAGTC 2 cut(s) 280, 674
ScrFI CCNGG 4 cut(s) 221, 402, 488, 586
SduI GDGCHC 3 cut(s) 48, 211, 764
SexAI ACCWGGT 1 cut(s) 400
SfaNI GCATC 2 cut(s) 544, 557
SfcI CTRYAG 1 cut(s) 558
SfuI TTCGAA 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 14, 281
SmoI CTYRAG 2 cut(s) 14, 281
SpeI ACTAGT 1 cut(s) 892
SsiI CCGC 2 cut(s) 100, 247
SspI AATATT 1 cut(s) 376
SspMI CTAG 2 cut(s) 261, 893
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 4 cut(s) 219, 400, 486, 584
StyI CCWWGG 2 cut(s) 24, 1011
TaaI ACNGT 2 cut(s) 416, 601
TaiI ACGT 3 cut(s) 245, 486, 759
TaqI TCGA 3 cut(s) 269, 751, 1034
TatI WGTACW 2 cut(s) 360, 889
TfiI GAWTC 5 cut(s) 182, 491, 780, 1016, 1063
Tru1I TTAA 3 cut(s) 134, 446, 885
Tru9I TTAA 3 cut(s) 134, 446, 885
TseI GCWGC 2 cut(s) 557, 560
TspDTI ATGAA 6 cut(s) 17, 518, 803, 824, 876, 1001
TspGWI ACGGA 1 cut(s) 908
VneI GTGCAC 1 cut(s) 207
XapI RAATTY 4 cut(s) 224, 316, 535, 1025
XmnI GAANNNNTTC 2 cut(s) 456, 509
XspI CTAG 2 cut(s) 261, 893
ZrmI AGTACT 1 cut(s) 362
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.