Rh2DG544800

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004058
Physical Location & Seq
Reverse (-)
77384620 .. 77387383
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG544800.1

Sequence Viewer

Length: 1164 bp
ATGTTGTTCCCATCTCTTCTCATAAACCCTAAATTGAGGTTCCGTCCCCAGAAACGCAGCAAATATGAAGGGTCTCTTTTGATAGTGAAATTGAAGATGAAGGGTCTTCTCAAGAAATCCAAGGCACGCAAGCCTTTGGAGCTCGTCAAGCACACGCGCGAGCTTCTCATCTTCTTCAATGGCAACATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGATGGAAGAATTAACAAAATCAACGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCGAATAAAGACACTTGTGCACAGCTTACCCAGGAATTTTTCAAAGATGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGAGACTCGTAAAAACGCAACACATATTGTCGCAAATTTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTACTTGGAAAAGAATATTGATCTTATAGATATTCTGATACCTGGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTCGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTGGATCGCTCAAACTCTGCTGTGATGGTTCGATACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGATCCAAACAAGACAATCCAATTGGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAATGAAAATAAGCCTCCTCAGATTGTTAATGTACTAGTAACAAATAGGAGCAAGCTTCTCCGTTTCTTTGGCAACTTCACCATTGAAAAAGACCATATGTACTTCGCTGCAGAGGATCAGCAGTTTGAAGCAGACAAAGTTGAACTTATGAAAGAGATTTCTATTCTTGAACCCAAGGATATTAAGGAATTTTCGTCAATCAGTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

387

Amino Acids

45.22

Weight (kDa)

9.18

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 37 - 374 1.1e-120 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 157, 159, 198, 439
AciI CCGC 2 cut(s) 196, 343
AclI AACGTT 1 cut(s) 242
AclWI GGATC 4 cut(s) 828, 893, 906, 1077
AcsI RAATTY 4 cut(s) 320, 412, 631, 1142
AfaI GTAC 4 cut(s) 458, 578, 987, 1055
AfiI CCNNNNNNNGG 2 cut(s) 503, 895
AflIII ACRYGT 1 cut(s) 852
AhlI ACTAGT 1 cut(s) 988
AjnI CCWGG 4 cut(s) 315, 496, 582, 680
AluBI AGCT 8 cut(s) 142, 163, 310, 370, 673, 799, 943, 1009
AluI AGCT 8 cut(s) 142, 163, 310, 370, 673, 799, 943, 1009
Alw21I GWGCWC 3 cut(s) 144, 307, 860
Alw26I GTCTC 5 cut(s) 78, 374, 501, 768, 917
Alw44I GTGCAC 1 cut(s) 303
AlwI GGATC 4 cut(s) 828, 893, 906, 1077
AlwNI CAGNNNCTG 1 cut(s) 757
ApaLI GTGCAC 1 cut(s) 303
ApeKI GCWGC 4 cut(s) 57, 653, 656, 1061
ApoI RAATTY 4 cut(s) 320, 412, 631, 1142
Asp700I GAANNNNTTC 3 cut(s) 552, 605, 608
AspLEI GCGC 1 cut(s) 159
AsuHPI GGTGA 4 cut(s) 287, 524, 769, 1024
AsuII TTCGAA 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 307
BamHI GGATCC 1 cut(s) 898
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 98, 928
Bbv12I GWGCWC 3 cut(s) 144, 307, 860
BbvI GCAGC 4 cut(s) 69, 640, 668, 1048
BccI CCATC 4 cut(s) 19, 214, 266, 835
BceAI ACGGC 1 cut(s) 800
BcgI CGANNNNNNTGC 2 cut(s) 837, 871
BciT130I CCWGG 4 cut(s) 317, 498, 584, 682
BcoDI GTCTC 5 cut(s) 78, 374, 501, 768, 917
BcuI ACTAGT 1 cut(s) 988
BfaI CTAG 2 cut(s) 357, 989
BfmI CTRYAG 2 cut(s) 654, 1062
BisI GCNGC 4 cut(s) 58, 654, 657, 1062
BlsI GCNGC 4 cut(s) 59, 655, 658, 1063
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 4 cut(s) 317, 498, 584, 682
BmiI GGNNCC 2 cut(s) 41, 900
BmrFI CCNGG 4 cut(s) 317, 498, 584, 682
BmsI GCATC 2 cut(s) 640, 653
BmtI GCTAGC 1 cut(s) 360
BpiI GAAGAC 2 cut(s) 98, 928
BpmI CTGGAG 1 cut(s) 779
Bpu14I TTCGAA 1 cut(s) 365
BpuEI CTTGAG 2 cut(s) 95, 398
BsaAI YACGTR 1 cut(s) 853
BsaI GGTCTC 2 cut(s) 78, 917
BsaJI CCNNGG 3 cut(s) 120, 315, 1128
Bsc4I CCNNNNNNNGG 2 cut(s) 503, 895
Bse1I ACTGG 2 cut(s) 731, 822
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 4 cut(s) 317, 498, 584, 682
BseDI CCNNGG 3 cut(s) 120, 315, 1128
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 503, 895
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 4 cut(s) 444, 779, 907, 986
BseNI ACTGG 2 cut(s) 731, 822
BseRI GAGGAG 1 cut(s) 960
BseSI GKGCMC 1 cut(s) 307
BseXI GCAGC 4 cut(s) 69, 640, 668, 1048
Bsh1236I CGCG 4 cut(s) 157, 159, 198, 439
BsiHKAI GWGCWC 3 cut(s) 144, 307, 860
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 503, 895
BsmAI GTCTC 5 cut(s) 78, 374, 501, 768, 917
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 2 cut(s) 552, 554
Bso31I GGTCTC 2 cut(s) 78, 917
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 3 cut(s) 144, 307, 860
Bsp143I GATC 4 cut(s) 475, 820, 898, 1069
Bsp68I TCGCGA 1 cut(s) 439
BspACI CCGC 2 cut(s) 196, 343
BspCNI CTCAG 4 cut(s) 445, 778, 906, 985
BspFNI CGCG 4 cut(s) 157, 159, 198, 439
BspLI GGNNCC 2 cut(s) 41, 900
BspMAI CTGCAG 2 cut(s) 658, 1066
BspOI GCTAGC 1 cut(s) 360
BspPI GGATC 4 cut(s) 828, 893, 906, 1077
BspT104I TTCGAA 1 cut(s) 365
BspTNI GGTCTC 2 cut(s) 78, 917
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 2 cut(s) 731, 822
BssECI CCNNGG 3 cut(s) 120, 315, 1128
BssMI GATC 4 cut(s) 475, 820, 898, 1069
BssT1I CCWWGG 2 cut(s) 120, 1128
Bst2UI CCWGG 4 cut(s) 317, 498, 584, 682
Bst4CI ACNGT 2 cut(s) 512, 697
Bst6I CTCTTC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 650
BstBAI YACGTR 1 cut(s) 853
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 7 cut(s) 127, 131, 161, 200, 358, 789, 1007
BstDEI CTNAG 4 cut(s) 453, 765, 893, 972
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 4 cut(s) 157, 159, 198, 439
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 4 cut(s) 478, 823, 901, 1072
BstMAI GTCTC 5 cut(s) 78, 374, 501, 768, 917
BstMBI GATC 4 cut(s) 475, 820, 898, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 139, 148, 650
BstNI CCWGG 4 cut(s) 317, 498, 584, 682
BstSCI CCNGG 4 cut(s) 315, 496, 582, 680
BstSFI CTRYAG 2 cut(s) 654, 1062
BstSLI GKGCMC 1 cut(s) 307
BstUI CGCG 4 cut(s) 157, 159, 198, 439
BstV1I GCAGC 4 cut(s) 69, 640, 668, 1048
BstV2I GAAGAC 2 cut(s) 98, 928
BstX2I RGATCY 1 cut(s) 898
BstYI RGATCY 1 cut(s) 898
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 1 cut(s) 655
BtuMI TCGCGA 1 cut(s) 439
Cac8I GCNNGC 7 cut(s) 127, 131, 161, 200, 358, 789, 1007
CaiI CAGNNNCTG 1 cut(s) 757
CfoI GCGC 1 cut(s) 159
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 4 cut(s) 457, 577, 986, 1054
CviAII CATG 4 cut(s) 187, 598, 871, 932
CviQI GTAC 4 cut(s) 457, 577, 986, 1054
DdeI CTNAG 4 cut(s) 453, 765, 893, 972
DpnI GATC 4 cut(s) 477, 822, 900, 1071
DpnII GATC 4 cut(s) 475, 820, 898, 1069
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 332
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 120, 1128
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 2 cut(s) 78, 917
Eco53kI GAGCTC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 142
EcoRII CCWGG 4 cut(s) 315, 496, 582, 680
EcoT14I CCWWGG 2 cut(s) 120, 1128
EcoT22I ATGCAT 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 2 cut(s) 120, 1128
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 4 cut(s) 190, 601, 874, 935
FalI AAGNNNNNCTT 6 cut(s) 60, 92, 593, 625, 1083, 1115
FaqI GGGAC 1 cut(s) 30
FatI CATG 4 cut(s) 186, 597, 870, 931
FauNDI CATATG 1 cut(s) 1050
Fnu4HI GCNGC 4 cut(s) 58, 654, 657, 1062
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 4 cut(s) 58, 654, 657, 1062
FspBI CTAG 2 cut(s) 357, 989
GlaI GCGC 1 cut(s) 158
GluI GCNGC 4 cut(s) 58, 654, 657, 1062
GsuI CTGGAG 1 cut(s) 779
HhaI GCGC 1 cut(s) 159
Hin1II CATG 4 cut(s) 190, 601, 874, 935
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 5 cut(s) 278, 382, 587, 761, 876
HphI GGTGA 4 cut(s) 287, 524, 769, 1024
Hpy166II GTNNAC 2 cut(s) 305, 693
Hpy188I TCNGA 7 cut(s) 283, 454, 492, 592, 649, 768, 975
Hpy188III TCNNGA 3 cut(s) 112, 438, 1121
Hpy8I GTNNAC 2 cut(s) 305, 693
HpyAV CCTTC 4 cut(s) 62, 94, 356, 899
HpyCH4III ACNGT 2 cut(s) 512, 697
HpyCH4IV ACGT 4 cut(s) 242, 338, 579, 852
HpyCH4V TGCA 8 cut(s) 305, 418, 536, 552, 656, 701, 953, 1064
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 148, 650
HpyF3I CTNAG 4 cut(s) 453, 765, 893, 972
HpySE526I ACGT 4 cut(s) 242, 338, 579, 852
Hsp92II CATG 4 cut(s) 190, 601, 874, 935
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 4 cut(s) 475, 820, 898, 1069
LmnI GCTCC 3 cut(s) 139, 670, 1002
Lsp1109I GCAGC 4 cut(s) 69, 640, 668, 1048
LweI GCATC 2 cut(s) 640, 653
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 2 cut(s) 357, 989
MaeII ACGT 4 cut(s) 242, 338, 579, 852
MaeIII GTNAC 2 cut(s) 813, 991
MalI GATC 4 cut(s) 477, 822, 900, 1071
MboI GATC 4 cut(s) 475, 820, 898, 1069
MboII GAAGA 9 cut(s) 8, 98, 106, 163, 166, 236, 601, 613, 928
MfeI CAATTG 1 cut(s) 917
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 3 cut(s) 144, 307, 860
MlyI GAGTC 2 cut(s) 376, 770
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 5 cut(s) 30, 567, 978, 981, 1060
Mph1103I ATGCAT 1 cut(s) 554
MroXI GAANNNNTTC 3 cut(s) 552, 605, 608
MseI TTAA 4 cut(s) 230, 542, 981, 1137
MspR9I CCNGG 4 cut(s) 317, 498, 584, 682
MunI CAATTG 1 cut(s) 917
Mva1269I GAATGC 2 cut(s) 552, 554
MvaI CCWGG 4 cut(s) 317, 498, 584, 682
MvnI CGCG 4 cut(s) 157, 159, 198, 439
MwoI GCNNNNNNNGC 3 cut(s) 139, 148, 650
NdeI CATATG 1 cut(s) 1050
NdeII GATC 4 cut(s) 475, 820, 898, 1069
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 4 cut(s) 190, 601, 874, 935
NlaIV GGNNCC 2 cut(s) 41, 900
NruI TCGCGA 1 cut(s) 439
NsiI ATGCAT 1 cut(s) 554
NspV TTCGAA 1 cut(s) 365
PctI GAATGC 2 cut(s) 552, 554
PdmI GAANNNNTTC 3 cut(s) 552, 605, 608
PfeI GAWTC 3 cut(s) 278, 587, 876
PfoI TCCNGGA 1 cut(s) 582
PkrI GCNGC 4 cut(s) 59, 655, 658, 1063
PleI GAGTC 2 cut(s) 376, 769
PpsI GAGTC 2 cut(s) 376, 769
Ppu21I YACGTR 1 cut(s) 853
Psp124BI GAGCTC 1 cut(s) 144
Psp1406I AACGTT 1 cut(s) 242
Psp6I CCWGG 4 cut(s) 315, 496, 582, 680
PspGI CCWGG 4 cut(s) 315, 496, 582, 680
PspN4I GGNNCC 2 cut(s) 41, 900
PstI CTGCAG 2 cut(s) 658, 1066
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 1 cut(s) 898
RruI TCGCGA 1 cut(s) 439
RsaI GTAC 4 cut(s) 458, 578, 987, 1055
RsaNI GTAC 4 cut(s) 457, 577, 986, 1054
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 4 cut(s) 230, 542, 981, 1137
SatI GCNGC 4 cut(s) 58, 654, 657, 1062
Sau3AI GATC 4 cut(s) 475, 820, 898, 1069
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 2 cut(s) 376, 770
ScrFI CCNGG 4 cut(s) 317, 498, 584, 682
SduI GDGCHC 3 cut(s) 144, 307, 860
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 2 cut(s) 640, 653
SfcI CTRYAG 2 cut(s) 654, 1062
SfuI TTCGAA 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 110, 377
SmoI CTYRAG 2 cut(s) 110, 377
SpeI ACTAGT 1 cut(s) 988
SsiI CCGC 2 cut(s) 196, 343
SspI AATATT 1 cut(s) 472
SspMI CTAG 2 cut(s) 357, 989
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 4 cut(s) 315, 496, 582, 680
StyI CCWWGG 2 cut(s) 120, 1128
TaaI ACNGT 2 cut(s) 512, 697
TaiI ACGT 4 cut(s) 245, 341, 582, 855
TaqI TCGA 3 cut(s) 365, 612, 847
TatI WGTACW 3 cut(s) 456, 985, 1053
TfiI GAWTC 3 cut(s) 278, 587, 876
Tru1I TTAA 4 cut(s) 230, 542, 981, 1137
Tru9I TTAA 4 cut(s) 230, 542, 981, 1137
TseI GCWGC 4 cut(s) 57, 653, 656, 1061
TspDTI ATGAA 7 cut(s) 81, 113, 614, 899, 920, 972, 1118
TspGWI ACGGA 2 cut(s) 32, 1004
VneI GTGCAC 1 cut(s) 303
XapI RAATTY 4 cut(s) 320, 412, 631, 1142
XmnI GAANNNNTTC 3 cut(s) 552, 605, 608
XspI CTAG 2 cut(s) 357, 989
ZrmI AGTACT 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.