Rroxscaffold_2G00092630

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004058
Physical Location & Seq
Forward (+)
14169503 .. 14172088
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092630.1

Sequence Viewer

Length: 804 bp
ATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGATGGAAGAATTAACAAAATCAATGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCGAACAAAGACACCTGTGCACAGCTTACCCAGGAATTTTTCAAAGACGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGGGACTCGTAAAAACGCAACACATATTGTGGCAAATTTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTACTTGGAAAAGAATATTGATCTTATAGATATTCTGATACCTGGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCCAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTACATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTCGCACAATTTTTGTCTAAGAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTGGATCGCTCAAACTCTGCTGTGATGGTTCGATACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGATCCGAACAAGACAATCCAATTGGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAATGAAAATAAGCCTCCCAGATTGTTAATGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

267

Amino Acids

31.18

Weight (kDa)

8.29

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 4 - 263 1.4e-104 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 12, 253
AciI CCGC 2 cut(s) 10, 157
AclWI GGATC 3 cut(s) 642, 707, 720
AcsI RAATTY 3 cut(s) 134, 226, 445
AfaI GTAC 3 cut(s) 272, 392, 800
AfiI CCNNNNNNNGG 2 cut(s) 317, 709
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 5 cut(s) 142, 248, 452, 481, 754
AjnI CCWGG 5 cut(s) 129, 310, 386, 396, 494
AluBI AGCT 5 cut(s) 124, 184, 487, 613, 757
AluI AGCT 5 cut(s) 124, 184, 487, 613, 757
Alw21I GWGCWC 2 cut(s) 121, 674
Alw26I GTCTC 3 cut(s) 315, 582, 731
Alw44I GTGCAC 1 cut(s) 117
AlwI GGATC 3 cut(s) 642, 707, 720
AlwNI CAGNNNCTG 1 cut(s) 571
ApaLI GTGCAC 1 cut(s) 117
ApeKI GCWGC 2 cut(s) 467, 470
ApoI RAATTY 3 cut(s) 134, 226, 445
Asp700I GAANNNNTTC 2 cut(s) 366, 419
AsuHPI GGTGA 3 cut(s) 101, 338, 583
AsuII TTCGAA 1 cut(s) 179
AsuNHI GCTAGC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 121
BamHI GGATCC 1 cut(s) 712
BbsI GAAGAC 1 cut(s) 742
Bbv12I GWGCWC 2 cut(s) 121, 674
BbvI GCAGC 2 cut(s) 454, 482
BccI CCATC 3 cut(s) 28, 80, 649
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 651, 685
BciT130I CCWGG 5 cut(s) 131, 312, 388, 398, 496
BcoDI GTCTC 3 cut(s) 315, 582, 731
BfaI CTAG 2 cut(s) 171, 802
BfmI CTRYAG 1 cut(s) 468
BisI GCNGC 2 cut(s) 468, 471
BlsI GCNGC 2 cut(s) 469, 472
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 5 cut(s) 131, 312, 388, 398, 496
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 5 cut(s) 131, 312, 388, 398, 496
BmsI GCATC 2 cut(s) 454, 467
BmtI GCTAGC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 742
BpmI CTGGAG 1 cut(s) 593
Bpu14I TTCGAA 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 667
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 2 cut(s) 129, 190
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 709
Bse1I ACTGG 2 cut(s) 545, 636
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 5 cut(s) 131, 312, 388, 398, 496
BseDI CCNNGG 2 cut(s) 129, 190
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 2 cut(s) 317, 709
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 3 cut(s) 258, 593, 721
BseNI ACTGG 2 cut(s) 545, 636
BseSI GKGCMC 1 cut(s) 121
BseXI GCAGC 2 cut(s) 454, 482
Bsh1236I CGCG 2 cut(s) 12, 253
BsiHKAI GWGCWC 2 cut(s) 121, 674
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 2 cut(s) 317, 709
BsmAI GTCTC 3 cut(s) 315, 582, 731
BsmBI CGTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 2 cut(s) 366, 368
Bso31I GGTCTC 1 cut(s) 731
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 121, 674
Bsp143I GATC 3 cut(s) 289, 634, 712
Bsp68I TCGCGA 1 cut(s) 253
BspACI CCGC 2 cut(s) 10, 157
BspCNI CTCAG 3 cut(s) 259, 592, 720
BspFNI CGCG 2 cut(s) 12, 253
BspLI GGNNCC 1 cut(s) 714
BspMAI CTGCAG 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 3 cut(s) 642, 707, 720
BspT104I TTCGAA 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 165
BsrI ACTGG 2 cut(s) 545, 636
BssECI CCNNGG 2 cut(s) 129, 190
BssMI GATC 3 cut(s) 289, 634, 712
BssT1I CCWWGG 1 cut(s) 190
Bst2UI CCWGG 5 cut(s) 131, 312, 388, 398, 496
Bst4CI ACNGT 2 cut(s) 326, 511
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 667
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 14, 172, 603
BstDEI CTNAG 4 cut(s) 267, 528, 579, 707
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 2 cut(s) 12, 253
BstKTI GATC 3 cut(s) 292, 637, 715
BstMAI GTCTC 3 cut(s) 315, 582, 731
BstMBI GATC 3 cut(s) 289, 634, 712
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstNI CCWGG 5 cut(s) 131, 312, 388, 398, 496
BstSCI CCNGG 5 cut(s) 129, 310, 386, 396, 494
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 121
BstUI CGCG 2 cut(s) 12, 253
BstV1I GCAGC 2 cut(s) 454, 482
BstV2I GAAGAC 1 cut(s) 742
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BtgZI GCGATG 1 cut(s) 99
BtsCI GGATG 1 cut(s) 469
BtuMI TCGCGA 1 cut(s) 253
Cac8I GCNNGC 3 cut(s) 14, 172, 603
CaiI CAGNNNCTG 1 cut(s) 571
CsiI ACCWGGT 1 cut(s) 310
Csp6I GTAC 3 cut(s) 271, 391, 799
CviAII CATG 3 cut(s) 412, 685, 746
CviQI GTAC 3 cut(s) 271, 391, 799
DdeI CTNAG 4 cut(s) 267, 528, 579, 707
DpnI GATC 3 cut(s) 291, 636, 714
DpnII GATC 3 cut(s) 289, 634, 712
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 190
Eco31I GGTCTC 1 cut(s) 731
EcoRII CCWGG 5 cut(s) 129, 310, 386, 396, 494
EcoT14I CCWWGG 1 cut(s) 190
EcoT22I ATGCAT 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 190
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 3 cut(s) 415, 688, 749
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 208
FatI CATG 3 cut(s) 411, 684, 745
Fnu4HI GCNGC 2 cut(s) 468, 471
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 468, 471
FspBI CTAG 2 cut(s) 171, 802
GluI GCNGC 2 cut(s) 468, 471
GsuI CTGGAG 1 cut(s) 593
Hin1II CATG 3 cut(s) 415, 688, 749
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 5 cut(s) 92, 196, 401, 575, 690
HphI GGTGA 3 cut(s) 101, 338, 583
Hpy166II GTNNAC 2 cut(s) 119, 507
Hpy188I TCNGA 7 cut(s) 97, 268, 306, 406, 463, 582, 717
Hpy188III TCNNGA 1 cut(s) 252
Hpy8I GTNNAC 2 cut(s) 119, 507
HpyAV CCTTC 2 cut(s) 170, 713
HpyCH4III ACNGT 2 cut(s) 326, 511
HpyCH4IV ACGT 3 cut(s) 152, 393, 666
HpyCH4V TGCA 6 cut(s) 119, 232, 350, 366, 470, 767
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 4 cut(s) 267, 528, 579, 707
HpySE526I ACGT 3 cut(s) 152, 393, 666
Hsp92II CATG 3 cut(s) 415, 688, 749
Kzo9I GATC 3 cut(s) 289, 634, 712
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 2 cut(s) 454, 482
LweI GCATC 2 cut(s) 454, 467
MabI ACCWGGT 1 cut(s) 310
MaeI CTAG 2 cut(s) 171, 802
MaeII ACGT 3 cut(s) 152, 393, 666
MaeIII GTNAC 1 cut(s) 627
MalI GATC 3 cut(s) 291, 636, 714
MboI GATC 3 cut(s) 289, 634, 712
MboII GAAGA 3 cut(s) 50, 427, 742
MfeI CAATTG 1 cut(s) 731
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 2 cut(s) 121, 674
MlyI GAGTC 2 cut(s) 190, 584
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 1 cut(s) 792
Mph1103I ATGCAT 1 cut(s) 368
MroXI GAANNNNTTC 2 cut(s) 366, 419
MseI TTAA 3 cut(s) 44, 356, 794
MspR9I CCNGG 5 cut(s) 131, 312, 388, 398, 496
MunI CAATTG 1 cut(s) 731
Mva1269I GAATGC 2 cut(s) 366, 368
MvaI CCWGG 5 cut(s) 131, 312, 388, 398, 496
MvnI CGCG 2 cut(s) 12, 253
MwoI GCNNNNNNNGC 1 cut(s) 464
NdeII GATC 3 cut(s) 289, 634, 712
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 3 cut(s) 415, 688, 749
NlaIV GGNNCC 1 cut(s) 714
NruI TCGCGA 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 368
NspV TTCGAA 1 cut(s) 179
PctI GAATGC 2 cut(s) 366, 368
PdmI GAANNNNTTC 2 cut(s) 366, 419
PfeI GAWTC 3 cut(s) 92, 401, 690
PfoI TCCNGGA 1 cut(s) 396
PkrI GCNGC 2 cut(s) 469, 472
PleI GAGTC 2 cut(s) 190, 583
PpsI GAGTC 2 cut(s) 190, 583
Ppu21I YACGTR 1 cut(s) 667
Psp6I CCWGG 5 cut(s) 129, 310, 386, 396, 494
PspGI CCWGG 5 cut(s) 129, 310, 386, 396, 494
PspN4I GGNNCC 1 cut(s) 714
PstI CTGCAG 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 571
PsuI RGATCY 1 cut(s) 712
RruI TCGCGA 1 cut(s) 253
RsaI GTAC 3 cut(s) 272, 392, 800
RsaNI GTAC 3 cut(s) 271, 391, 799
SaqAI TTAA 3 cut(s) 44, 356, 794
SatI GCNGC 2 cut(s) 468, 471
Sau3AI GATC 3 cut(s) 289, 634, 712
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 2 cut(s) 190, 584
ScrFI CCNGG 5 cut(s) 131, 312, 388, 398, 496
SduI GDGCHC 2 cut(s) 121, 674
SexAI ACCWGGT 1 cut(s) 310
SfaNI GCATC 2 cut(s) 454, 467
SfcI CTRYAG 1 cut(s) 468
SfuI TTCGAA 1 cut(s) 179
SsiI CCGC 2 cut(s) 10, 157
SspI AATATT 1 cut(s) 286
SspMI CTAG 2 cut(s) 171, 802
StyD4I CCNGG 5 cut(s) 129, 310, 386, 396, 494
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 2 cut(s) 326, 511
TaiI ACGT 3 cut(s) 155, 396, 669
TaqI TCGA 2 cut(s) 179, 661
TatI WGTACW 2 cut(s) 270, 798
TfiI GAWTC 3 cut(s) 92, 401, 690
Tru1I TTAA 3 cut(s) 44, 356, 794
Tru9I TTAA 3 cut(s) 44, 356, 794
TseI GCWGC 2 cut(s) 467, 470
TspDTI ATGAA 4 cut(s) 428, 713, 734, 786
VneI GTGCAC 1 cut(s) 117
XapI RAATTY 3 cut(s) 134, 226, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmnI GAANNNNTTC 2 cut(s) 366, 419
XspI CTAG 2 cut(s) 171, 802
ZrmI AGTACT 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.