Rh2BG535800

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004058
Physical Location & Seq
Reverse (-)
74460754 .. 74463689
2936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG535800.1

Sequence Viewer

Length: 933 bp
ATGGATTCTGAAGCTCTATCTCAATCCCATATGTTGTTCCCATCTCTTCTCATAAACCCTAAATTGAGGTTCCGTCCCCAGAAACGCAGCAAATATGAAGGGTCTCTTTTGATAGTGAAATTGAAGATGAAGGGTCTTCTCAAGAAATCCAAGGCACGCAAGCCTTTGGAGCTCGTCAAGCACACGCGCGAGCTTCTCATCTTCTTCAATGGCAACATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGATGGAAGAATTAACAAAATCAACGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCGAATAAAGACACTTGTGCACAGCTTACCCAGGAATTTTTCAAAGATGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGAGACTCGTAAAAACGCAACACATATTGTCGCAAATTTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTACTTGGAAAAGAATATTGATCTTATAGATATTCTGATACCTGGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTCGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTGGATCGCTCAAACTCTGCTGTGATGGTTCGATACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

310

Amino Acids

36.1

Weight (kDa)

9.36

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 47 - 309 9e-92 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 187, 189, 228, 469
AciI CCGC 2 cut(s) 226, 373
AclI AACGTT 1 cut(s) 272
AclWI GGATC 1 cut(s) 858
AcsI RAATTY 3 cut(s) 350, 442, 661
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 2 cut(s) 488, 608
AfiI CCNNNNNNNGG 2 cut(s) 533, 925
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 6 cut(s) 124, 208, 358, 464, 668, 697
AjnI CCWGG 4 cut(s) 345, 526, 612, 710
AluBI AGCT 7 cut(s) 14, 172, 193, 340, 400, 703, 829
AluI AGCT 7 cut(s) 14, 172, 193, 340, 400, 703, 829
Alw21I GWGCWC 3 cut(s) 174, 337, 890
Alw26I GTCTC 4 cut(s) 108, 404, 531, 798
Alw44I GTGCAC 1 cut(s) 333
AlwI GGATC 1 cut(s) 858
AlwNI CAGNNNCTG 1 cut(s) 787
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 3 cut(s) 87, 683, 686
ApoI RAATTY 3 cut(s) 350, 442, 661
Asp700I GAANNNNTTC 3 cut(s) 582, 635, 638
AspLEI GCGC 1 cut(s) 189
AsuHPI GGTGA 3 cut(s) 317, 554, 799
AsuII TTCGAA 1 cut(s) 395
AsuNHI GCTAGC 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 3 cut(s) 174, 337, 890
BbvI GCAGC 3 cut(s) 99, 670, 698
BccI CCATC 4 cut(s) 49, 244, 296, 865
BceAI ACGGC 1 cut(s) 830
BcgI CGANNNNNNTGC 2 cut(s) 867, 901
BciT130I CCWGG 4 cut(s) 347, 528, 614, 712
BcoDI GTCTC 4 cut(s) 108, 404, 531, 798
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 684
BisI GCNGC 3 cut(s) 88, 684, 687
BlsI GCNGC 3 cut(s) 89, 685, 688
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 4 cut(s) 347, 528, 614, 712
BmiI GGNNCC 1 cut(s) 71
BmrFI CCNGG 4 cut(s) 347, 528, 614, 712
BmsI GCATC 2 cut(s) 670, 683
BmtI GCTAGC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 809
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 125, 428
BsaAI YACGTR 1 cut(s) 883
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 150, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 925
Bse1I ACTGG 2 cut(s) 761, 852
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 4 cut(s) 347, 528, 614, 712
BseDI CCNNGG 2 cut(s) 150, 345
BseGI GGATG 1 cut(s) 685
BseLI CCNNNNNNNGG 2 cut(s) 533, 925
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 474, 809
BseNI ACTGG 2 cut(s) 761, 852
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 3 cut(s) 99, 670, 698
Bsh1236I CGCG 4 cut(s) 187, 189, 228, 469
BsiHKAI GWGCWC 3 cut(s) 174, 337, 890
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 533, 925
BsmAI GTCTC 4 cut(s) 108, 404, 531, 798
BsmBI CGTCTC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 60
BsmI GAATGC 2 cut(s) 582, 584
Bso31I GGTCTC 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 3 cut(s) 174, 337, 890
Bsp143I GATC 2 cut(s) 505, 850
Bsp68I TCGCGA 1 cut(s) 469
BspACI CCGC 2 cut(s) 226, 373
BspCNI CTCAG 2 cut(s) 475, 808
BspFNI CGCG 4 cut(s) 187, 189, 228, 469
BspLI GGNNCC 1 cut(s) 71
BspMAI CTGCAG 1 cut(s) 688
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 1 cut(s) 858
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 2 cut(s) 761, 852
BssECI CCNNGG 2 cut(s) 150, 345
BssMI GATC 2 cut(s) 505, 850
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 4 cut(s) 347, 528, 614, 712
Bst4CI ACNGT 2 cut(s) 542, 727
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 680
BstBAI YACGTR 1 cut(s) 883
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 6 cut(s) 157, 161, 191, 230, 388, 819
BstDEI CTNAG 3 cut(s) 483, 795, 923
BstF5I GGATG 1 cut(s) 685
BstFNI CGCG 4 cut(s) 187, 189, 228, 469
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 2 cut(s) 508, 853
BstMAI GTCTC 4 cut(s) 108, 404, 531, 798
BstMBI GATC 2 cut(s) 505, 850
BstMWI GCNNNNNNNGC 3 cut(s) 169, 178, 680
BstNI CCWGG 4 cut(s) 347, 528, 614, 712
BstSCI CCNGG 4 cut(s) 345, 526, 612, 710
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 4 cut(s) 187, 189, 228, 469
BstV1I GCAGC 3 cut(s) 99, 670, 698
BstV2I GAAGAC 1 cut(s) 128
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 685
BtuMI TCGCGA 1 cut(s) 469
Cac8I GCNNGC 6 cut(s) 157, 161, 191, 230, 388, 819
CaiI CAGNNNCTG 1 cut(s) 787
CfoI GCGC 1 cut(s) 189
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 2 cut(s) 487, 607
CviAII CATG 3 cut(s) 217, 628, 901
CviQI GTAC 2 cut(s) 487, 607
DdeI CTNAG 3 cut(s) 483, 795, 923
DpnI GATC 2 cut(s) 507, 852
DpnII GATC 2 cut(s) 505, 850
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 172
EcoRII CCWGG 4 cut(s) 345, 526, 612, 710
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 3 cut(s) 220, 631, 904
FalI AAGNNNNNCTT 4 cut(s) 90, 122, 623, 655
FaqI GGGAC 1 cut(s) 60
FatI CATG 3 cut(s) 216, 627, 900
FauNDI CATATG 1 cut(s) 30
Fnu4HI GCNGC 3 cut(s) 88, 684, 687
FokI GGATG 1 cut(s) 692
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 3 cut(s) 88, 684, 687
FspBI CTAG 1 cut(s) 387
GlaI GCGC 1 cut(s) 188
GluI GCNGC 3 cut(s) 88, 684, 687
GsuI CTGGAG 1 cut(s) 809
HhaI GCGC 1 cut(s) 189
Hin1II CATG 3 cut(s) 220, 631, 904
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HinfI GANTC 6 cut(s) 5, 308, 412, 617, 791, 906
HphI GGTGA 3 cut(s) 317, 554, 799
Hpy166II GTNNAC 2 cut(s) 335, 723
Hpy188I TCNGA 7 cut(s) 10, 313, 484, 522, 622, 679, 798
Hpy188III TCNNGA 2 cut(s) 142, 468
Hpy8I GTNNAC 2 cut(s) 335, 723
HpyAV CCTTC 4 cut(s) 92, 124, 386, 929
HpyCH4III ACNGT 2 cut(s) 542, 727
HpyCH4IV ACGT 4 cut(s) 272, 368, 609, 882
HpyCH4V TGCA 6 cut(s) 335, 448, 566, 582, 686, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 178, 680
HpyF3I CTNAG 3 cut(s) 483, 795, 923
HpySE526I ACGT 4 cut(s) 272, 368, 609, 882
Hsp92II CATG 3 cut(s) 220, 631, 904
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 2 cut(s) 505, 850
LmnI GCTCC 2 cut(s) 169, 700
Lsp1109I GCAGC 3 cut(s) 99, 670, 698
LweI GCATC 2 cut(s) 670, 683
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 1 cut(s) 387
MaeII ACGT 4 cut(s) 272, 368, 609, 882
MaeIII GTNAC 1 cut(s) 843
MalI GATC 2 cut(s) 507, 852
MboI GATC 2 cut(s) 505, 850
MboII GAAGA 8 cut(s) 38, 128, 136, 193, 196, 266, 631, 643
MhlI GDGCHC 3 cut(s) 174, 337, 890
MlyI GAGTC 2 cut(s) 406, 800
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 2 cut(s) 60, 597
Mph1103I ATGCAT 1 cut(s) 584
MroXI GAANNNNTTC 3 cut(s) 582, 635, 638
MseI TTAA 2 cut(s) 260, 572
MspR9I CCNGG 4 cut(s) 347, 528, 614, 712
Mva1269I GAATGC 2 cut(s) 582, 584
MvaI CCWGG 4 cut(s) 347, 528, 614, 712
MvnI CGCG 4 cut(s) 187, 189, 228, 469
MwoI GCNNNNNNNGC 3 cut(s) 169, 178, 680
NdeI CATATG 1 cut(s) 30
NdeII GATC 2 cut(s) 505, 850
NheI GCTAGC 1 cut(s) 386
NlaIII CATG 3 cut(s) 220, 631, 904
NlaIV GGNNCC 1 cut(s) 71
NruI TCGCGA 1 cut(s) 469
NsiI ATGCAT 1 cut(s) 584
NspV TTCGAA 1 cut(s) 395
PctI GAATGC 2 cut(s) 582, 584
PdmI GAANNNNTTC 3 cut(s) 582, 635, 638
PfeI GAWTC 4 cut(s) 5, 308, 617, 906
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 3 cut(s) 89, 685, 688
PleI GAGTC 2 cut(s) 406, 799
PpsI GAGTC 2 cut(s) 406, 799
Ppu21I YACGTR 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 174
Psp1406I AACGTT 1 cut(s) 272
Psp6I CCWGG 4 cut(s) 345, 526, 612, 710
PspGI CCWGG 4 cut(s) 345, 526, 612, 710
PspN4I GGNNCC 1 cut(s) 71
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 1 cut(s) 787
RruI TCGCGA 1 cut(s) 469
RsaI GTAC 2 cut(s) 488, 608
RsaNI GTAC 2 cut(s) 487, 607
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 2 cut(s) 260, 572
SatI GCNGC 3 cut(s) 88, 684, 687
Sau3AI GATC 2 cut(s) 505, 850
ScaI AGTACT 1 cut(s) 488
SchI GAGTC 2 cut(s) 406, 800
ScrFI CCNGG 4 cut(s) 347, 528, 614, 712
SduI GDGCHC 3 cut(s) 174, 337, 890
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 2 cut(s) 670, 683
SfcI CTRYAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 140, 407
SmoI CTYRAG 2 cut(s) 140, 407
SsiI CCGC 2 cut(s) 226, 373
SspI AATATT 1 cut(s) 502
SspMI CTAG 1 cut(s) 387
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 4 cut(s) 345, 526, 612, 710
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 542, 727
TaiI ACGT 4 cut(s) 275, 371, 612, 885
TaqI TCGA 3 cut(s) 395, 642, 877
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 4 cut(s) 5, 308, 617, 906
Tru1I TTAA 2 cut(s) 260, 572
Tru9I TTAA 2 cut(s) 260, 572
TseI GCWGC 3 cut(s) 87, 683, 686
TspDTI ATGAA 4 cut(s) 111, 143, 644, 929
TspGWI ACGGA 1 cut(s) 62
VneI GTGCAC 1 cut(s) 333
XapI RAATTY 3 cut(s) 350, 442, 661
XmnI GAANNNNTTC 3 cut(s) 582, 635, 638
XspI CTAG 1 cut(s) 387
ZrmI AGTACT 1 cut(s) 488
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.