MD05G1359100.v1.1

MO25-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004058
Physical Location & Seq
Forward (+)
47407182 .. 47410821
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1359100.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGAAGGGGCTGTTCAAGTCCAAGCCCAGAACCCCCGCCGACATAGTTCGGCAGACCCGAGATCTCCTCGTCTATGCTCAGCGCGCTCCCGATTCTCGTGAAAGCAAACGCGAGGAAAAAATGTCAGAGCTCTGTAAAAACATCAGGGAGCTGAAGTCGATTCTCTATGGAAATAGCGAGTCTGAACCCGTCGCAGAAGCATGTGCGCAGTTGACTCAGGAGTTTTTTAAGGAGAACACACTTCGACTTCTTATTACATGTCTTCCGAAATTGAACTTGGAGGCTCGAAAAGATGCCACTCAAGTGGTTGCAAACTTGCAGAGGCAACAAGTTCAGTCTAAGCTGATTGCATGTGATTACTTGGAAGCCAACATTGATTTGATGGATATTTTGATCCAAGGGTATGGAAACACTGACATGGCTCTACATTATGGTGCAATGTTGAGGGAGTGCATACGTCACCAAAGTGTTGCAAGATATGTTTTGGAATCACAACACATGAAGAAGTTTTTTGATTATATACAGCTCCCCAATTTTGATATTGCTGCAGATGCTGCTGCAACTTTTAAGGAACTCTTGACGAGGCACAAATCTACTGTAGCGGATTTTCTTTCCAAGAACTATGATTGGTTTTTTGCGGAGTATAACTCAAAGCTACTGGAATCCTCCAATTATATTACCAGACGACAAGCTGTCAAGTTGTTAGGAGATATTTTGTTGGATCGGTCAAACTCAGCTGTGATGACTCGATACGTGAGCTCAAGGGACAACTTGAGGATCCTTATGAATCTTCTCAGAGAATCAAGCAAGAGCATTCAGATTGAAGCATTTCATGTTTTCAAGTTGTTTGCTGCTAATCAAAATAAACCCGCCGACATTGTGAGCATACTTGTTGCCAATAGAAGCAAGCTTTTACGGCTGTTTGCTGATTTTAAGATAGATAAAGAGGATGAACAATTTGAGGCAGACAAGGCTCAAGTAGTGAGAGAAATAGCTGCCATGGAACCCAAAGACTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

340

Amino Acids

39.02

Weight (kDa)

8.63

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 4 - 334 1.1e-134 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 207
AccII CGCG 2 cut(s) 84, 111
AciI CCGC 4 cut(s) 36, 602, 638, 870
AclWI GGATC 4 cut(s) 388, 729, 772, 785
AcuI CTGAAG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 257
AgsI TTSAA 4 cut(s) 16, 274, 824, 841
AhdI GACNNNNNGTC 1 cut(s) 692
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AleI CACNNNNGTG 2 cut(s) 302, 465
Alw21I GWGCWC 2 cut(s) 132, 761
AlwI GGATC 4 cut(s) 388, 729, 772, 785
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 57
ApeKI GCWGC 5 cut(s) 545, 554, 557, 851, 995
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 3 cut(s) 84, 86, 208
AsuHPI GGTGA 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 57
BamHI GGATCC 1 cut(s) 777
BanII GRGCYC 2 cut(s) 132, 761
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 132, 761
BbvI GCAGC 5 cut(s) 532, 541, 544, 838, 982
BccI CCATC 1 cut(s) 376
BceAI ACGGC 1 cut(s) 932
BfmI CTRYAG 2 cut(s) 546, 597
BglII AGATCT 1 cut(s) 61
BisI GCNGC 5 cut(s) 546, 555, 558, 852, 996
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 5 cut(s) 547, 556, 559, 853, 997
BmeRI GACNNNNNGTC 1 cut(s) 692
BmeT110I CYCGRG 1 cut(s) 57
BmiI GGNNCC 2 cut(s) 779, 1005
BmsI GCATC 2 cut(s) 283, 541
BpiI GAAGAC 1 cut(s) 254
BplI GAGNNNNNCTC 4 cut(s) 51, 83, 632, 664
Bpu1102I GCTNAGC 1 cut(s) 78
BpuEI CTTGAG 4 cut(s) 285, 745, 793, 960
BsaAI YACGTR 1 cut(s) 754
BsaJI CCNNGG 2 cut(s) 397, 999
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bse1I ACTGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 397, 999
BseGI GGATG 1 cut(s) 955
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 4 cut(s) 92, 230, 747, 808
BseNI ACTGG 1 cut(s) 663
BsePI GCGCGC 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 5 cut(s) 532, 541, 544, 838, 982
Bsh1236I CGCG 2 cut(s) 84, 111
BsiHKAI GWGCWC 2 cut(s) 132, 761
BsiHKCI CYCGRG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 813
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 132, 761
Bsp143I GATC 4 cut(s) 61, 393, 721, 777
Bsp1720I GCTNAGC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 999
BspACI CCGC 4 cut(s) 36, 602, 638, 870
BspCNI CTCAG 4 cut(s) 91, 229, 746, 807
BspFNI CGCG 2 cut(s) 84, 111
BspHI TCATGA 1 cut(s) 1016
BspLI GGNNCC 2 cut(s) 779, 1005
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 4 cut(s) 388, 729, 772, 785
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 1 cut(s) 663
BssECI CCNNGG 2 cut(s) 397, 999
BssHII GCGCGC 1 cut(s) 82
BssMI GATC 4 cut(s) 61, 393, 721, 777
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 397, 999
Bst2BI CACGAG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 598
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 84, 908
BstDEI CTNAG 5 cut(s) 78, 216, 339, 733, 794
BstDSI CCRYGG 1 cut(s) 999
BstF5I GGATG 1 cut(s) 955
BstFNI CGCG 2 cut(s) 84, 111
BstHHI GCGC 3 cut(s) 84, 86, 208
BstKTI GATC 4 cut(s) 64, 396, 724, 780
BstMBI GATC 4 cut(s) 61, 393, 721, 777
BstMWI GCNNNNNNNGC 5 cut(s) 83, 551, 554, 916, 971
BstNSI RCATGY 3 cut(s) 204, 261, 354
BstSFI CTRYAG 2 cut(s) 546, 597
BstUI CGCG 2 cut(s) 84, 111
BstV1I GCAGC 5 cut(s) 532, 541, 544, 838, 982
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 2 cut(s) 61, 777
BstXI CCANNNNNNTGG 2 cut(s) 304, 404
BstYI RGATCY 2 cut(s) 61, 777
BtgI CCRYGG 1 cut(s) 999
BtsCI GGATG 1 cut(s) 955
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 84, 908
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 1016
CfoI GCGC 3 cut(s) 84, 86, 208
CviAII CATG 8 cut(s) 201, 258, 351, 418, 499, 833, 1000, 1017
DdeI CTNAG 5 cut(s) 78, 216, 339, 733, 794
DpnI GATC 4 cut(s) 63, 395, 723, 779
DpnII GATC 4 cut(s) 61, 393, 721, 777
DriI GACNNNNNGTC 1 cut(s) 692
Eam1105I GACNNNNNGTC 1 cut(s) 692
Ecl136II GAGCTC 2 cut(s) 130, 759
Eco130I CCWWGG 2 cut(s) 397, 999
Eco24I GRGCYC 2 cut(s) 132, 761
Eco53kI GAGCTC 2 cut(s) 130, 759
Eco57I CTGAAG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 57
EcoICRI GAGCTC 2 cut(s) 130, 759
EcoT14I CCWWGG 2 cut(s) 397, 999
EcoT38I GRGCYC 2 cut(s) 132, 761
ErhI CCWWGG 2 cut(s) 397, 999
FaeI CATG 8 cut(s) 204, 261, 354, 421, 502, 836, 1003, 1020
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 779
FatI CATG 8 cut(s) 200, 257, 350, 417, 498, 832, 999, 1016
FauI CCCGC 2 cut(s) 43, 877
Fnu4HI GCNGC 5 cut(s) 546, 555, 558, 852, 996
FokI GGATG 1 cut(s) 962
FriOI GRGCYC 2 cut(s) 132, 761
Fsp4HI GCNGC 5 cut(s) 546, 555, 558, 852, 996
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 3 cut(s) 83, 85, 207
GluI GCNGC 5 cut(s) 546, 555, 558, 852, 996
HhaI GCGC 3 cut(s) 84, 86, 208
Hin1II CATG 8 cut(s) 204, 261, 354, 421, 502, 836, 1003, 1020
Hin6I GCGC 3 cut(s) 82, 84, 206
HinP1I GCGC 3 cut(s) 82, 84, 206
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HindIII AAGCTT 1 cut(s) 908
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 5 cut(s) 127, 184, 267, 797, 819
Hpy188III TCNNGA 5 cut(s) 89, 98, 218, 577, 1017
Hpy8I GTNNAC 1 cut(s) 213
Hpy99I CGWCG 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4IV ACGT 2 cut(s) 457, 753
HpyCH4V TGCA 8 cut(s) 311, 319, 350, 437, 453, 473, 548, 560
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 551, 554, 916, 971
HpyF3I CTNAG 5 cut(s) 78, 216, 339, 733, 794
HpySE526I ACGT 2 cut(s) 457, 753
Hsp92II CATG 8 cut(s) 204, 261, 354, 421, 502, 836, 1003, 1020
HspAI GCGC 3 cut(s) 82, 84, 206
Kzo9I GATC 4 cut(s) 61, 393, 721, 777
LmnI GCTCC 3 cut(s) 91, 148, 531
LpnPI CCDG 5 cut(s) 40, 130, 203, 644, 694
Lsp1109I GCAGC 5 cut(s) 532, 541, 544, 838, 982
LweI GCATC 2 cut(s) 283, 541
MaeII ACGT 2 cut(s) 457, 753
MaeIII GTNAC 1 cut(s) 458
MalI GATC 4 cut(s) 63, 395, 723, 779
MboI GATC 4 cut(s) 61, 393, 721, 777
MboII GAAGA 3 cut(s) 254, 514, 782
MflI RGATCY 2 cut(s) 61, 777
MhlI GDGCHC 2 cut(s) 132, 761
MluCI AATT 4 cut(s) 269, 532, 670, 956
MlyI GAGTC 4 cut(s) 188, 208, 739, 1007
MmeI TCCRAC 1 cut(s) 699
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 3 cut(s) 228, 567, 933
MslI CAYNNNNRTG 4 cut(s) 302, 416, 432, 465
MspA1I CMGCKG 1 cut(s) 737
Mva1269I GAATGC 1 cut(s) 813
MvnI CGCG 2 cut(s) 84, 111
MwoI GCNNNNNNNGC 5 cut(s) 83, 551, 554, 916, 971
NcoI CCATGG 1 cut(s) 999
NdeII GATC 4 cut(s) 61, 393, 721, 777
NlaIII CATG 8 cut(s) 204, 261, 354, 421, 502, 836, 1003, 1020
NlaIV GGNNCC 2 cut(s) 779, 1005
NmuCI GTSAC 1 cut(s) 458
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 3 cut(s) 204, 261, 354
OliI CACNNNNGTG 2 cut(s) 302, 465
PagI TCATGA 1 cut(s) 1016
PauI GCGCGC 1 cut(s) 82
PciI ACATGT 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 6 cut(s) 92, 160, 488, 662, 787, 800
PkrI GCNGC 5 cut(s) 547, 556, 559, 853, 997
PleI GAGTC 4 cut(s) 187, 208, 739, 1007
PpsI GAGTC 4 cut(s) 187, 208, 739, 1007
Ppu21I YACGTR 1 cut(s) 754
PscI ACATGT 1 cut(s) 257
Psp124BI GAGCTC 2 cut(s) 132, 761
PspN4I GGNNCC 2 cut(s) 779, 1005
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 61, 777
PteI GCGCGC 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 737
RseI CAYNNNNRTG 4 cut(s) 302, 416, 432, 465
SacI GAGCTC 2 cut(s) 132, 761
SaqAI TTAA 3 cut(s) 228, 567, 933
SatI GCNGC 5 cut(s) 546, 555, 558, 852, 996
Sau3AI GATC 4 cut(s) 61, 393, 721, 777
SchI GAGTC 4 cut(s) 188, 208, 739, 1007
SduI GDGCHC 2 cut(s) 132, 761
SfaNI GCATC 2 cut(s) 283, 541
SfcI CTRYAG 2 cut(s) 546, 597
SmiMI CAYNNNNRTG 4 cut(s) 302, 416, 432, 465
SmlI CTYRAG 4 cut(s) 300, 760, 772, 975
SmoI CTYRAG 4 cut(s) 300, 760, 772, 975
Sse9I AATT 4 cut(s) 269, 532, 670, 956
SsiI CCGC 4 cut(s) 36, 602, 638, 870
SstI GAGCTC 2 cut(s) 132, 761
StyI CCWWGG 2 cut(s) 397, 999
TaaI ACNGT 1 cut(s) 598
TaiI ACGT 2 cut(s) 460, 756
TaqI TCGA 4 cut(s) 158, 244, 286, 748
TaqII GACCGA 1 cut(s) 714
TasI AATT 4 cut(s) 269, 532, 670, 956
TfiI GAWTC 6 cut(s) 92, 160, 488, 662, 787, 800
Tru1I TTAA 3 cut(s) 228, 567, 933
Tru9I TTAA 3 cut(s) 228, 567, 933
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 5 cut(s) 545, 554, 557, 851, 995
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 5 cut(s) 17, 515, 800, 821, 966
TspRI CASTG 1 cut(s) 418
XceI RCATGY 3 cut(s) 204, 261, 354
XmnI GAANNNNTTC 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.