MD17G1111400.v1.1

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004058
Physical Location & Seq
Forward (+)
9559485 .. 9561870
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1111400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGATGACAAAAAAGTTGAATATGAAAACTCTTTTCAAGCCAAAGACGAGAATGCCGATAGAGCTTGTAAAGCACATGCATGAGCTTTTGATTTTTATTGATCAAAACACCGATGCGCGTGAGCAAAAGCGAAAGGAAAAGATGGAAGAATTGACAAAAGCAATATTGGAGATAAGAACTGTCGTATATGGAGATGATGATTCAGAACCAAATAAAGATTCTTGTGCACAACTTACCCGAGAGTTTTTTAGGGAAGACACTTTCAGGCTCCTCATTGTTTGTCTTTCAAAGCTCAACTTGGGGGATCGTCAAAACGCCACTCATGTCATTGCAAATTTGCAAAGGCAAAAGGTTCAGTCGCGTATAATCGCATTAGAGTATTTGGAGAATAATATTGATATTATGGATATTCTGATAAAAGGCTACGAAGACAATAGTGATATTGCTTTATCTTATGGTGCAATTACAAGGGAATGCATTCGTCATCAGAATATTGCAAGATATGTACTAGAATCAGACCACGTGAAGGAGTTTTTTTATTACATTCAAACTCCAAATTTCGACGTAGCATCGGATGCTGCAACTACTTTCAAGGAGCTTATGACTAGGCACAAATCAACTGTTGCTGAATTCCTTTCTAAGAACTATGAGTGGTTTTTCCATGAGTACAATTCACAGTTGTTGGAGTCTCCCAATTATATCACCAAACGCCAGGCTATCAAGTTGTTAGGAGATATGTTATTGGATCGTACAAACTCTGATGTGATGGTTCGATATGTATGCTCTTTGCATAACATGATCATCCTAATGAACCTTCTCAGGGATCCAAAGAAGACAATCAAGTTAGAAACCTTTCAGGTCTTCAAGCTCTTTGTTGCAAACCAAAATAAGCCTCCTGAGATTGTTAATGTACTTGTCAAAAATCGAACCAAGCTTATTCGTTTCCTTAACGACTTCACCATTGAGAAAGAGGATAAGCAGTTTGAAGCAAACAAAGCTCAAGTTATTAATGAGATTTCTGTTCTTAAACTCAAACCGAGCAATCCCAAGATGTCATCGGTCTGCAGCTTTAGAACCAATTGTGCGATTCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

366

Amino Acids

42.83

Weight (kDa)

8.77

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 11 - 342 2e-118 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 118, 361
AclWI GGATC 4 cut(s) 312, 753, 818, 831
AcsI RAATTY 3 cut(s) 334, 556, 629
AcvI CACGTG 1 cut(s) 523
AfaI GTAC 4 cut(s) 507, 668, 751, 912
AfiI CCNNNNNNNGG 2 cut(s) 526, 820
AgsI TTSAA 8 cut(s) 19, 37, 288, 548, 592, 865, 986, 1091
AjnI CCWGG 1 cut(s) 711
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AluBI AGCT 8 cut(s) 64, 85, 292, 598, 868, 934, 998, 1068
AluI AGCT 8 cut(s) 64, 85, 292, 598, 868, 934, 998, 1068
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 1 cut(s) 693
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 4 cut(s) 312, 753, 818, 831
Ama87I CYCGRG 1 cut(s) 237
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 2 cut(s) 578, 1065
ApoI RAATTY 3 cut(s) 334, 556, 629
AseI ATTAAT 1 cut(s) 1008
Asp700I GAANNNNTTC 2 cut(s) 477, 852
AspLEI GCGC 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 694, 949
AvaI CYCGRG 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 229
BamHI GGATCC 1 cut(s) 823
BbrPI CACGTG 1 cut(s) 523
BbsI GAAGAC 4 cut(s) 261, 435, 839, 853
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 2 cut(s) 565, 1077
BccI CCATC 2 cut(s) 136, 760
BcgI CGANNNNNNTGC 2 cut(s) 762, 796
BciT130I CCWGG 1 cut(s) 713
BclI TGATCA 2 cut(s) 100, 798
BcoDI GTCTC 1 cut(s) 693
BfaI CTAG 2 cut(s) 509, 606
BfmI CTRYAG 1 cut(s) 1063
BisI GCNGC 2 cut(s) 579, 1066
BlsI GCNGC 2 cut(s) 580, 1067
Bme1390I CCNGG 1 cut(s) 713
BmeT110I CYCGRG 1 cut(s) 237
BmiI GGNNCC 2 cut(s) 269, 825
BmrFI CCNGG 1 cut(s) 713
BmsI GCATC 3 cut(s) 103, 565, 578
BpiI GAAGAC 4 cut(s) 261, 435, 839, 853
BpuEI CTTGAG 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 523
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 820
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 1 cut(s) 713
BseGI GGATG 2 cut(s) 580, 801
BseLI CCNNNNNNNGG 2 cut(s) 526, 820
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 832, 888
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 2 cut(s) 565, 1077
Bsh1236I CGCG 2 cut(s) 118, 361
BsiHKAI GWGCWC 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 526, 820
BsmAI GTCTC 1 cut(s) 693
BsmI GAATGC 3 cut(s) 57, 477, 479
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 5 cut(s) 100, 304, 745, 798, 823
BspCNI CTCAG 2 cut(s) 831, 889
BspFNI CGCG 2 cut(s) 118, 361
BspLI GGNNCC 2 cut(s) 269, 825
BspMAI CTGCAG 1 cut(s) 1067
BspPI GGATC 4 cut(s) 312, 753, 818, 831
BsrDI GCAATG 1 cut(s) 327
BssMI GATC 5 cut(s) 100, 304, 745, 798, 823
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 3 cut(s) 181, 622, 678
BstAPI GCANNNNNTGC 1 cut(s) 575
BstBAI YACGTR 1 cut(s) 523
BstDEI CTNAG 3 cut(s) 639, 818, 897
BstF5I GGATG 2 cut(s) 580, 801
BstFNI CGCG 2 cut(s) 118, 361
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 5 cut(s) 103, 307, 748, 801, 826
BstMAI GTCTC 1 cut(s) 693
BstMBI GATC 5 cut(s) 100, 304, 745, 798, 823
BstMWI GCNNNNNNNGC 4 cut(s) 61, 70, 575, 995
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 711
BstSFI CTRYAG 1 cut(s) 1063
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 2 cut(s) 118, 361
BstV1I GCAGC 2 cut(s) 565, 1077
BstV2I GAAGAC 4 cut(s) 261, 435, 839, 853
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BtsCI GGATG 2 cut(s) 580, 801
CfoI GCGC 1 cut(s) 118
Csp6I GTAC 4 cut(s) 506, 667, 750, 911
CviAII CATG 5 cut(s) 76, 80, 323, 662, 796
CviQI GTAC 4 cut(s) 506, 667, 750, 911
DdeI CTNAG 3 cut(s) 639, 818, 897
DpnI GATC 5 cut(s) 102, 306, 747, 800, 825
DpnII GATC 5 cut(s) 100, 304, 745, 798, 823
Eco72I CACGTG 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 711
EcoT22I ATGCAT 2 cut(s) 81, 479
FaeI CATG 5 cut(s) 79, 83, 326, 665, 799
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FatI CATG 5 cut(s) 75, 79, 322, 661, 795
FbaI TGATCA 2 cut(s) 100, 798
Fnu4HI GCNGC 2 cut(s) 579, 1066
FokI GGATG 2 cut(s) 587, 788
Fsp4HI GCNGC 2 cut(s) 579, 1066
FspBI CTAG 2 cut(s) 509, 606
GlaI GCGC 1 cut(s) 117
GluI GCNGC 2 cut(s) 579, 1066
HhaI GCGC 1 cut(s) 118
Hin1II CATG 5 cut(s) 79, 83, 326, 665, 799
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 5 cut(s) 200, 218, 512, 686, 1087
HphI GGTGA 2 cut(s) 694, 949
Hpy166II GTNNAC 1 cut(s) 227
Hpy188I TCNGA 6 cut(s) 205, 414, 489, 517, 574, 760
Hpy188III TCNNGA 1 cut(s) 896
Hpy8I GTNNAC 1 cut(s) 227
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 2 cut(s) 520, 824
HpyCH4III ACNGT 3 cut(s) 181, 622, 678
HpyCH4IV ACGT 2 cut(s) 522, 564
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 70, 575, 995
HpyF3I CTNAG 3 cut(s) 639, 818, 897
HpySE526I ACGT 2 cut(s) 522, 564
Hsp92II CATG 5 cut(s) 79, 83, 326, 665, 799
HspAI GCGC 1 cut(s) 116
Ksp22I TGATCA 2 cut(s) 100, 798
Kzo9I GATC 5 cut(s) 100, 304, 745, 798, 823
LmnI GCTCC 2 cut(s) 273, 595
LpnPI CCDG 6 cut(s) 250, 698, 725, 805, 842, 909
Lsp1109I GCAGC 2 cut(s) 565, 1077
LweI GCATC 3 cut(s) 103, 565, 578
MaeI CTAG 2 cut(s) 509, 606
MaeII ACGT 2 cut(s) 522, 564
MalI GATC 5 cut(s) 102, 306, 747, 800, 825
MboI GATC 5 cut(s) 100, 304, 745, 798, 823
MboII GAAGA 5 cut(s) 158, 266, 440, 844, 853
MfeI CAATTG 1 cut(s) 1078
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 8 cut(s) 149, 334, 462, 556, 629, 670, 694, 1078
MlyI GAGTC 1 cut(s) 695
MmeI TCCRAC 1 cut(s) 663
MnlI CCTC 3 cut(s) 281, 903, 964
Mph1103I ATGCAT 2 cut(s) 81, 479
MroXI GAANNNNTTC 2 cut(s) 477, 852
MseI TTAA 5 cut(s) 906, 948, 1008, 1026, 1096
MslI CAYNNNNRTG 2 cut(s) 78, 806
MspR9I CCNGG 1 cut(s) 713
MunI CAATTG 1 cut(s) 1078
Mva1269I GAATGC 3 cut(s) 57, 477, 479
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 2 cut(s) 118, 361
MwoI GCNNNNNNNGC 4 cut(s) 61, 70, 575, 995
NdeII GATC 5 cut(s) 100, 304, 745, 798, 823
NlaIII CATG 5 cut(s) 79, 83, 326, 665, 799
NlaIV GGNNCC 2 cut(s) 269, 825
NsiI ATGCAT 2 cut(s) 81, 479
NspI RCATGY 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 3 cut(s) 57, 477, 479
PdmI GAANNNNTTC 2 cut(s) 477, 852
PfeI GAWTC 4 cut(s) 200, 218, 512, 1087
PkrI GCNGC 2 cut(s) 580, 1067
PleI GAGTC 1 cut(s) 694
PmaCI CACGTG 1 cut(s) 523
PmlI CACGTG 1 cut(s) 523
PpsI GAGTC 1 cut(s) 694
Ppu21I YACGTR 1 cut(s) 523
PshBI ATTAAT 1 cut(s) 1008
Psp6I CCWGG 1 cut(s) 711
PspCI CACGTG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 269, 825
PstI CTGCAG 1 cut(s) 1067
PsuI RGATCY 1 cut(s) 823
RsaI GTAC 4 cut(s) 507, 668, 751, 912
RsaNI GTAC 4 cut(s) 506, 667, 750, 911
RseI CAYNNNNRTG 2 cut(s) 78, 806
SaqAI TTAA 5 cut(s) 906, 948, 1008, 1026, 1096
SatI GCNGC 2 cut(s) 579, 1066
Sau3AI GATC 5 cut(s) 100, 304, 745, 798, 823
SchI GAGTC 1 cut(s) 695
ScrFI CCNGG 1 cut(s) 713
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 103, 565, 578
SfcI CTRYAG 1 cut(s) 1063
SmiMI CAYNNNNRTG 2 cut(s) 78, 806
SmlI CTYRAG 1 cut(s) 999
SmoI CTYRAG 1 cut(s) 999
Sse9I AATT 8 cut(s) 149, 334, 462, 556, 629, 670, 694, 1078
SspI AATATT 3 cut(s) 165, 394, 493
SspMI CTAG 2 cut(s) 509, 606
StyD4I CCNGG 1 cut(s) 711
TaaI ACNGT 3 cut(s) 181, 622, 678
TaiI ACGT 2 cut(s) 525, 567
TaqI TCGA 3 cut(s) 561, 772, 925
TaqII GACCGA 1 cut(s) 1048
TasI AATT 8 cut(s) 149, 334, 462, 556, 629, 670, 694, 1078
TatI WGTACW 3 cut(s) 505, 666, 910
TfiI GAWTC 4 cut(s) 200, 218, 512, 1087
Tru1I TTAA 5 cut(s) 906, 948, 1008, 1026, 1096
Tru9I TTAA 5 cut(s) 906, 948, 1008, 1026, 1096
TseI GCWGC 2 cut(s) 578, 1065
TspDTI ATGAA 2 cut(s) 38, 824
VneI GTGCAC 1 cut(s) 225
VspI ATTAAT 1 cut(s) 1008
XapI RAATTY 3 cut(s) 334, 556, 629
XceI RCATGY 1 cut(s) 79
XmnI GAANNNNTTC 2 cut(s) 477, 852
XspI CTAG 2 cut(s) 509, 606
Zsp2I ATGCAT 2 cut(s) 81, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.