Rw5G000860

MO25-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004058
Physical Location & Seq
Reverse (-)
586649 .. 590556
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000860.1

Sequence Viewer

Length: 1023 bp
ATGAAGGGTCTCTTCAAGTCTAAGCCCCGCACGCCGGCCGACGTCGTCCGCCAGACCCGCGATCTCCTCATCTATGCTCAGCGCAGCCCCGATTCCCGCGAGAGCAAGCGTGAGGAAAAGATGGCAGAGCTTTGTAAAAACATTAGGGAGTTGAAGTGCATTCTCTATGGCAATAGTGAGTCAGAGCCTGTTTCGGAAGCTTGTGCGCAATTGACTCAGGAGTTTTTCAGGGAGGACACGCTTCGACTTTTAATCATGTGTGTTCCGAAGTTAAACTTGGAGGCAAGGAAAGATGCCACTCAAGTTGTTGCCAATCTACAAAGGCAACAGGTTCACTCCAAGTTGATTGCATCCGATTACTTGGAAGCAAACATTGATATAATGGATGTTTTGATCCAAGGGTACGGAGATACAGATATGGCTTTACATTATGGCGCAATGCTGAGAGAGTGCATACGCCACCAAAGCGTTGCAAGATATGTTTTGGAATCTCAACACATGAAGAAGTTCTTTGACTATATACAGCTTCCAAATTTCGATATTGCTGCAGATGCTGCTGCGACGTTTAAGGAACTCATGACAAGGCATAAATCTACAGTAGCAGATTTTTTATCCAAGAATTATGACTGGTTCTTTGCGGAGTATAACTCAAAGCTGCTGGAATCCTCCAATTACATTACCAGACGACAAGCTGTCAAGCTGTTAGGAGATATATTGCTGGATCGGTCAAATTCAGCTGTAATGACTCGATATGTGAGCTCAAGAGACAATTTGCGGATCCTTATGAATCTTCTCAGAGAGTCAAGCAAGAGCATCCAGATCGAAGCATTTCATGTTTTTAAGCTATTTGCTGCCAACCAAAATAAACCTGCCGACATCGTGGGCATACTTGTTGCAAATAGAAGCAAACTTCTACGCCTGTTTGCTGATTTTAAAATTGATAAAGAGGATGAACAATTTGAGGCAGACAAAGCTCAAGTAGTAAGAGAGATTGCTGCCTTGGAACCGAAAGAACTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

340

Amino Acids

39.18

Weight (kDa)

8.15

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 4 - 335 1.5e-133 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 877
AccII CGCG 2 cut(s) 60, 99
AciI CCGC 6 cut(s) 28, 49, 58, 97, 638, 775
AclWI GGATC 4 cut(s) 388, 729, 772, 785
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 532, 730
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 2 cut(s) 16, 154
AhdI GACNNNNNGTC 1 cut(s) 692
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
Alw21I GWGCWC 1 cut(s) 761
Alw26I GTCTC 2 cut(s) 14, 759
AlwI GGATC 4 cut(s) 388, 729, 772, 785
AlwNI CAGNNNCTG 2 cut(s) 188, 554
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 7 cut(s) 84, 545, 554, 557, 655, 851, 995
ApoI RAATTY 2 cut(s) 532, 730
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
Asp700I GAANNNNTTC 2 cut(s) 506, 828
AspLEI GCGC 3 cut(s) 84, 208, 437
BamHI GGATCC 1 cut(s) 777
BanII GRGCYC 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 761
BbvI GCAGC 7 cut(s) 96, 532, 541, 544, 642, 838, 982
BccI CCATC 1 cut(s) 115
BcgI CGANNNNNNTGC 4 cut(s) 527, 561, 802, 836
BcoDI GTCTC 2 cut(s) 14, 759
BfmI CTRYAG 2 cut(s) 546, 594
BfuAI ACCTGC 1 cut(s) 877
BisI GCNGC 7 cut(s) 85, 546, 555, 558, 656, 852, 996
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 7 cut(s) 86, 547, 556, 559, 657, 853, 997
BmeRI GACNNNNNGTC 1 cut(s) 692
BmiI GGNNCC 2 cut(s) 779, 1005
BmsI GCATC 4 cut(s) 283, 359, 541, 822
BplI GAGNNNNNCTC 2 cut(s) 632, 664
Bpu1102I GCTNAGC 1 cut(s) 78
BpuEI CTTGAG 3 cut(s) 285, 745, 960
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 397, 999
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 632
Bse3DI GCAATG 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 397, 999
BseGI GGATG 4 cut(s) 350, 391, 813, 955
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 4 cut(s) 92, 230, 434, 808
BseNI ACTGG 1 cut(s) 632
BseRI GAGGAG 1 cut(s) 56
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 7 cut(s) 96, 532, 541, 544, 642, 838, 982
Bsh1236I CGCG 2 cut(s) 60, 99
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 38
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 761
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 14, 759
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 761
Bsp143I GATC 5 cut(s) 61, 393, 721, 777, 819
Bsp1720I GCTNAGC 1 cut(s) 78
BspACI CCGC 6 cut(s) 28, 49, 58, 97, 638, 775
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 4 cut(s) 91, 229, 435, 807
BspFNI CGCG 2 cut(s) 60, 99
BspHI TCATGA 2 cut(s) 576, 1019
BspLI GGNNCC 2 cut(s) 779, 1005
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 877
BspPI GGATC 4 cut(s) 388, 729, 772, 785
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 1 cut(s) 632
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 397, 999
BssMI GATC 5 cut(s) 61, 393, 721, 777, 819
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 397, 999
Bst4CI ACNGT 1 cut(s) 598
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 3 cut(s) 32, 36, 107
BstDEI CTNAG 5 cut(s) 21, 78, 216, 443, 794
BstF5I GGATG 4 cut(s) 350, 391, 813, 955
BstFNI CGCG 2 cut(s) 60, 99
BstHHI GCGC 3 cut(s) 84, 208, 437
BstKTI GATC 5 cut(s) 64, 396, 724, 780, 822
BstMAI GTCTC 2 cut(s) 14, 759
BstMBI GATC 5 cut(s) 61, 393, 721, 777, 819
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 6 cut(s) 31, 57, 465, 551, 554, 971
BstSFI CTRYAG 2 cut(s) 546, 594
BstUI CGCG 2 cut(s) 60, 99
BstV1I GCAGC 7 cut(s) 96, 532, 541, 544, 642, 838, 982
BstX2I RGATCY 1 cut(s) 777
BstYI RGATCY 1 cut(s) 777
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 4 cut(s) 350, 391, 813, 955
BveI ACCTGC 1 cut(s) 877
Cac8I GCNNGC 3 cut(s) 32, 36, 107
CaiI CAGNNNCTG 2 cut(s) 188, 554
CciI TCATGA 2 cut(s) 576, 1019
CfoI GCGC 3 cut(s) 84, 208, 437
Cfr10I RCCGGY 1 cut(s) 34
Csp6I GTAC 1 cut(s) 403
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 5 cut(s) 256, 499, 577, 833, 1020
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 5 cut(s) 21, 78, 216, 443, 794
DpnI GATC 5 cut(s) 63, 395, 723, 779, 821
DpnII GATC 5 cut(s) 61, 393, 721, 777, 819
DraI TTTAAA 1 cut(s) 934
DriI GACNNNNNGTC 1 cut(s) 692
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 17
Eam1105I GACNNNNNGTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 759
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 397, 999
Eco24I GRGCYC 1 cut(s) 761
Eco31I GGTCTC 1 cut(s) 14
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 759
EcoICRI GAGCTC 1 cut(s) 759
EcoT14I CCWWGG 2 cut(s) 397, 999
EcoT38I GRGCYC 1 cut(s) 761
ErhI CCWWGG 2 cut(s) 397, 999
FaeI CATG 5 cut(s) 259, 502, 580, 836, 1023
FalI AAGNNNNNCTT 5 cut(s) 28, 260, 292, 494, 526
FatI CATG 5 cut(s) 255, 498, 576, 832, 1019
FauI CCCGC 3 cut(s) 35, 65, 104
Fnu4HI GCNGC 7 cut(s) 85, 546, 555, 558, 656, 852, 996
FokI GGATG 4 cut(s) 337, 398, 800, 962
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 7 cut(s) 85, 546, 555, 558, 656, 852, 996
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 3 cut(s) 83, 207, 436
GluI GCNGC 7 cut(s) 85, 546, 555, 558, 656, 852, 996
HaeIII GGCC 1 cut(s) 38
HapII CCGG 1 cut(s) 35
HhaI GCGC 3 cut(s) 84, 208, 437
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 5 cut(s) 259, 502, 580, 836, 1023
Hin6I GCGC 3 cut(s) 82, 206, 435
HinP1I GCGC 3 cut(s) 82, 206, 435
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 8 cut(s) 92, 179, 214, 488, 662, 745, 787, 800
HpaII CCGG 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 5 cut(s) 184, 196, 267, 355, 797
Hpy188III TCNNGA 5 cut(s) 218, 577, 762, 817, 1020
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 3 cut(s) 44, 47, 565
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4IV ACGT 2 cut(s) 42, 563
HpyCH4V TGCA 6 cut(s) 159, 350, 453, 473, 548, 896
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 57, 465, 551, 554, 971
HpyF3I CTNAG 5 cut(s) 21, 78, 216, 443, 794
HpySE526I ACGT 2 cut(s) 42, 563
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 5 cut(s) 259, 502, 580, 836, 1023
HspAI GCGC 3 cut(s) 82, 206, 435
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Kzo9I GATC 5 cut(s) 61, 393, 721, 777, 819
Lsp1109I GCAGC 7 cut(s) 96, 532, 541, 544, 642, 838, 982
LweI GCATC 4 cut(s) 283, 359, 541, 822
MaeII ACGT 2 cut(s) 42, 563
MalI GATC 5 cut(s) 63, 395, 723, 779, 821
MboI GATC 5 cut(s) 61, 393, 721, 777, 819
MboII GAAGA 3 cut(s) 4, 514, 782
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 1 cut(s) 777
MhlI GDGCHC 1 cut(s) 761
MluCI AATT 8 cut(s) 209, 532, 619, 670, 730, 769, 936, 956
MlyI GAGTC 4 cut(s) 188, 208, 739, 809
MnlI CCTC 7 cut(s) 77, 106, 226, 274, 676, 940, 955
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 2 cut(s) 506, 828
MseI TTAA 5 cut(s) 251, 272, 567, 840, 933
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 159
MvnI CGCG 2 cut(s) 60, 99
MwoI GCNNNNNNNGC 6 cut(s) 31, 57, 465, 551, 554, 971
NaeI GCCGGC 1 cut(s) 36
NdeII GATC 5 cut(s) 61, 393, 721, 777, 819
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 5 cut(s) 259, 502, 580, 836, 1023
NlaIV GGNNCC 2 cut(s) 779, 1005
NsbI TGCGCA 1 cut(s) 207
PagI TCATGA 2 cut(s) 576, 1019
PctI GAATGC 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 2 cut(s) 506, 828
PfeI GAWTC 4 cut(s) 92, 488, 662, 787
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 7 cut(s) 86, 547, 556, 559, 657, 853, 997
PleI GAGTC 4 cut(s) 187, 208, 739, 808
PpsI GAGTC 4 cut(s) 187, 208, 739, 808
Psp124BI GAGCTC 1 cut(s) 761
PspN4I GGNNCC 2 cut(s) 779, 1005
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 2 cut(s) 188, 554
PsuI RGATCY 1 cut(s) 777
PsyI GACNNNGTC 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SacI GAGCTC 1 cut(s) 761
SaqAI TTAA 5 cut(s) 251, 272, 567, 840, 933
SatI GCNGC 7 cut(s) 85, 546, 555, 558, 656, 852, 996
Sau3AI GATC 5 cut(s) 61, 393, 721, 777, 819
SchI GAGTC 4 cut(s) 188, 208, 739, 809
SduI GDGCHC 1 cut(s) 761
SfaNI GCATC 4 cut(s) 283, 359, 541, 822
SfcI CTRYAG 2 cut(s) 546, 594
SmlI CTYRAG 3 cut(s) 300, 760, 975
SmoI CTYRAG 3 cut(s) 300, 760, 975
Sse9I AATT 8 cut(s) 209, 532, 619, 670, 730, 769, 936, 956
SsiI CCGC 6 cut(s) 28, 49, 58, 97, 638, 775
SstI GAGCTC 1 cut(s) 761
StyI CCWWGG 2 cut(s) 397, 999
TaaI ACNGT 1 cut(s) 598
TaiI ACGT 2 cut(s) 45, 566
TaqI TCGA 4 cut(s) 244, 537, 748, 822
TaqII GACCGA 1 cut(s) 714
TasI AATT 8 cut(s) 209, 532, 619, 670, 730, 769, 936, 956
TfiI GAWTC 4 cut(s) 92, 488, 662, 787
Tru1I TTAA 5 cut(s) 251, 272, 567, 840, 933
Tru9I TTAA 5 cut(s) 251, 272, 567, 840, 933
TseI GCWGC 7 cut(s) 84, 545, 554, 557, 655, 851, 995
TspDTI ATGAA 5 cut(s) 17, 515, 800, 821, 966
TspGWI ACGGA 1 cut(s) 420
Tth111I GACNNNGTC 1 cut(s) 44
XapI RAATTY 2 cut(s) 532, 730
XmnI GAANNNNTTC 2 cut(s) 506, 828
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.