RLG00000020949

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004058
Physical Location & Seq
Reverse (-)
70437814 .. 70443205
5392 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020949

Sequence Viewer

Length: 1368 bp
ATGGGTGGCGACCCATTGGAGAGAGGCCCAGAAGTCCTCATAGCAAGTGGGTATGGGCCAGGTCGGGAAGAGGCCACACTGGACGGAGACCGCGATGACGTCGACGACAGAGTAGATGGTGGAGACATAGTCGTAGTAGGTGGAGGAGAAGGCGGACAGCGTAAGATATGGCTCCATCTCAATCCCATGTTGTTCCCATCTCTTCTCATAAACCCTAAATTGAGGTTCCGTCCCCAGATGCGAGGTAAGAAACGCAGCAAAGATGAAGGGTCTCTTTTGATAGTGAAATTGAAGATGAAGGGTCTTCTCAAGAAATCCAAGGCACGCAAGCCTTTGGAGCTCGTCAAGCACACGCGCGAGCTTCTCATCTTCTTCAATGGCAACATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGATGGAAGAATTAACAAAATCAATGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCAAATAAAGACGCTTGTGCACAGCTTACCCAGGAATTTTTCAAAGATGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGGGACTCGTAAAAACGCAACACATATTGTGGCAAATTTGCAGAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTACTTGGAAAAGAATACTGATCTTATAGATATTCTGATACCTGGTTACGAAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTTGATCGCTCAAACTCTGCTGTGATGGTTCGTTACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGATCCAAACAAGACAATCCAATTGGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAATGAAAATAAGCCTCCTCAGATTGTTAATGTACTAGTAACAAATAGGAGCAAGCTTCTCCGTTTCTTTGGCAACTTCACCATTGAAAAAGAGGATCAGCAGTTTGAAGCAGACAAAGTTGAACTTATGAAAGAGATTTCTATTCTTGAACCCAAGGATATTAAGGAATTTTCGTCAATCGTGAGCTTTAGAGAGAATTGTGAGATTCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

456

Amino Acids

52.32

Weight (kDa)

8.09

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 103 - 433 5.3e-124 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 102
AccII CGCG 5 cut(s) 93, 355, 357, 396, 637
AciI CCGC 4 cut(s) 91, 153, 394, 541
AclWI GGATC 3 cut(s) 1091, 1104, 1254
AcsI RAATTY 4 cut(s) 518, 610, 829, 1319
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 3 cut(s) 656, 776, 1185
AfiI CCNNNNNNNGG 1 cut(s) 1093
AflIII ACRYGT 1 cut(s) 1050
AhlI ACTAGT 1 cut(s) 1186
AjnI CCWGG 5 cut(s) 58, 513, 694, 780, 878
AluBI AGCT 9 cut(s) 340, 361, 508, 568, 871, 997, 1141, 1207, 1338
AluI AGCT 9 cut(s) 340, 361, 508, 568, 871, 997, 1141, 1207, 1338
Alw21I GWGCWC 3 cut(s) 342, 505, 1058
Alw26I GTCTC 5 cut(s) 81, 117, 276, 966, 1115
Alw44I GTGCAC 1 cut(s) 501
AlwI GGATC 3 cut(s) 1091, 1104, 1254
AlwNI CAGNNNCTG 1 cut(s) 955
AoxI GGCC 3 cut(s) 25, 56, 72
ApaLI GTGCAC 1 cut(s) 501
ApeKI GCWGC 3 cut(s) 255, 851, 854
ApoI RAATTY 4 cut(s) 518, 610, 829, 1319
Asp700I GAANNNNTTC 2 cut(s) 750, 803
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 2 cut(s) 26, 56
AsuHPI GGTGA 4 cut(s) 485, 722, 967, 1222
AsuII TTCGAA 1 cut(s) 563
AsuNHI GCTAGC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 505
BamHI GGATCC 1 cut(s) 1096
BanII GRGCYC 1 cut(s) 342
BbsI GAAGAC 3 cut(s) 296, 711, 1126
Bbv12I GWGCWC 3 cut(s) 342, 505, 1058
BbvI GCAGC 3 cut(s) 267, 838, 866
BccI CCATC 6 cut(s) 110, 183, 205, 412, 464, 1033
BceAI ACGGC 1 cut(s) 998
BciT130I CCWGG 5 cut(s) 60, 515, 696, 782, 880
BcoDI GTCTC 5 cut(s) 81, 117, 276, 966, 1115
BcuI ACTAGT 1 cut(s) 1186
BfaI CTAG 2 cut(s) 555, 1187
BfmI CTRYAG 1 cut(s) 852
BisI GCNGC 3 cut(s) 256, 852, 855
BlsI GCNGC 3 cut(s) 257, 853, 856
BmcAI AGTACT 1 cut(s) 656
Bme1390I CCNGG 5 cut(s) 60, 515, 696, 782, 880
BmgT120I GGNCC 2 cut(s) 26, 56
BmiI GGNNCC 3 cut(s) 173, 227, 1098
BmrFI CCNGG 5 cut(s) 60, 515, 696, 782, 880
BmsI GCATC 3 cut(s) 228, 838, 851
BmtI GCTAGC 1 cut(s) 558
BpiI GAAGAC 3 cut(s) 296, 711, 1126
BpmI CTGGAG 1 cut(s) 977
Bpu14I TTCGAA 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 293
BsaAI YACGTR 1 cut(s) 1051
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 3 cut(s) 81, 276, 1115
BsaJI CCNNGG 4 cut(s) 318, 513, 574, 1305
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 1093
Bse1I ACTGG 2 cut(s) 84, 929
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 5 cut(s) 60, 515, 696, 782, 880
BseDI CCNNGG 4 cut(s) 318, 513, 574, 1305
BseGI GGATG 1 cut(s) 853
BseLI CCNNNNNNNGG 1 cut(s) 1093
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 4 cut(s) 642, 977, 1105, 1184
BseNI ACTGG 2 cut(s) 84, 929
BseRI GAGGAG 2 cut(s) 159, 1158
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 3 cut(s) 267, 838, 866
Bsh1236I CGCG 5 cut(s) 93, 355, 357, 396, 637
BshFI GGCC 3 cut(s) 27, 58, 74
BsiHKAI GWGCWC 3 cut(s) 342, 505, 1058
BslFI GGGAC 2 cut(s) 216, 592
BslI CCNNNNNNNGG 1 cut(s) 1093
BsmAI GTCTC 5 cut(s) 81, 117, 276, 966, 1115
BsmFI GGGAC 2 cut(s) 216, 592
BsmI GAATGC 2 cut(s) 750, 752
BsnI GGCC 3 cut(s) 27, 58, 74
Bso31I GGTCTC 3 cut(s) 81, 276, 1115
Bsp119I TTCGAA 1 cut(s) 563
Bsp1286I GDGCHC 3 cut(s) 342, 505, 1058
Bsp143I GATC 4 cut(s) 673, 1018, 1096, 1246
Bsp68I TCGCGA 1 cut(s) 637
BspACI CCGC 4 cut(s) 91, 153, 394, 541
BspANI GGCC 3 cut(s) 27, 58, 74
BspCNI CTCAG 4 cut(s) 643, 976, 1104, 1183
BspFNI CGCG 5 cut(s) 93, 355, 357, 396, 637
BspLI GGNNCC 3 cut(s) 173, 227, 1098
BspMAI CTGCAG 1 cut(s) 856
BspOI GCTAGC 1 cut(s) 558
BspPI GGATC 3 cut(s) 1091, 1104, 1254
BspT104I TTCGAA 1 cut(s) 563
BspTNI GGTCTC 3 cut(s) 81, 276, 1115
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 2 cut(s) 84, 929
BssECI CCNNGG 4 cut(s) 318, 513, 574, 1305
BssMI GATC 4 cut(s) 673, 1018, 1096, 1246
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 3 cut(s) 318, 574, 1305
Bst2UI CCWGG 5 cut(s) 60, 515, 696, 782, 880
Bst4CI ACNGT 2 cut(s) 710, 895
Bst6I CTCTTC 2 cut(s) 63, 207
BstACI GRCGYC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 848
BstBAI YACGTR 1 cut(s) 1051
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 7 cut(s) 325, 329, 359, 398, 556, 987, 1205
BstDEI CTNAG 4 cut(s) 651, 963, 1091, 1170
BstF5I GGATG 1 cut(s) 853
BstFNI CGCG 5 cut(s) 93, 355, 357, 396, 637
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 4 cut(s) 676, 1021, 1099, 1249
BstMAI GTCTC 5 cut(s) 81, 117, 276, 966, 1115
BstMBI GATC 4 cut(s) 673, 1018, 1096, 1246
BstMWI GCNNNNNNNGC 3 cut(s) 337, 346, 848
BstNI CCWGG 5 cut(s) 60, 515, 696, 782, 880
BstSCI CCNGG 5 cut(s) 58, 513, 694, 780, 878
BstSFI CTRYAG 1 cut(s) 852
BstSLI GKGCMC 1 cut(s) 505
BstUI CGCG 5 cut(s) 93, 355, 357, 396, 637
BstV1I GCAGC 3 cut(s) 267, 838, 866
BstV2I GAAGAC 3 cut(s) 296, 711, 1126
BstX2I RGATCY 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1096
BsuRI GGCC 3 cut(s) 27, 58, 74
BtgZI GCGATG 2 cut(s) 108, 483
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 1 cut(s) 77
BtuMI TCGCGA 1 cut(s) 637
Cac8I GCNNGC 7 cut(s) 325, 329, 359, 398, 556, 987, 1205
CaiI CAGNNNCTG 1 cut(s) 955
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 2 cut(s) 26, 56
CseI GACGC 1 cut(s) 503
CsiI ACCWGGT 1 cut(s) 694
Csp6I GTAC 3 cut(s) 655, 775, 1184
CviAII CATG 6 cut(s) 187, 385, 796, 1069, 1130, 1362
CviQI GTAC 3 cut(s) 655, 775, 1184
DdeI CTNAG 4 cut(s) 651, 963, 1091, 1170
DpnI GATC 4 cut(s) 675, 1020, 1098, 1248
DpnII GATC 4 cut(s) 673, 1018, 1096, 1246
Eam1104I CTCTTC 2 cut(s) 63, 207
EarI CTCTTC 2 cut(s) 63, 207
EciI GGCGGA 2 cut(s) 168, 530
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 3 cut(s) 318, 574, 1305
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 3 cut(s) 81, 276, 1115
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 5 cut(s) 58, 513, 694, 780, 878
EcoT14I CCWWGG 3 cut(s) 318, 574, 1305
EcoT22I ATGCAT 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 3 cut(s) 318, 574, 1305
FaeI CATG 6 cut(s) 190, 388, 799, 1072, 1133, 1365
FalI AAGNNNNNCTT 6 cut(s) 258, 290, 791, 823, 1260, 1292
FaqI GGGAC 2 cut(s) 216, 592
FatI CATG 6 cut(s) 186, 384, 795, 1068, 1129, 1361
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 256, 852, 855
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 3 cut(s) 256, 852, 855
FspBI CTAG 2 cut(s) 555, 1187
GlaI GCGC 1 cut(s) 356
GluI GCNGC 3 cut(s) 256, 852, 855
GsuI CTGGAG 1 cut(s) 977
HaeIII GGCC 3 cut(s) 27, 58, 74
HgaI GACGC 1 cut(s) 503
HhaI GCGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 6 cut(s) 190, 388, 799, 1072, 1133, 1365
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HincII GTYRAC 2 cut(s) 103, 891
HindII GTYRAC 2 cut(s) 103, 891
HindIII AAGCTT 1 cut(s) 1205
HinfI GANTC 6 cut(s) 476, 580, 785, 959, 1074, 1357
HphI GGTGA 4 cut(s) 485, 722, 967, 1222
Hpy166II GTNNAC 3 cut(s) 103, 503, 891
Hpy188I TCNGA 7 cut(s) 481, 652, 690, 790, 847, 966, 1173
Hpy188III TCNNGA 5 cut(s) 65, 310, 636, 1298, 1333
Hpy8I GTNNAC 3 cut(s) 103, 503, 891
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 5 cut(s) 143, 260, 292, 554, 1097
HpyCH4III ACNGT 2 cut(s) 710, 895
HpyCH4IV ACGT 4 cut(s) 99, 536, 777, 1050
HpyCH4V TGCA 7 cut(s) 503, 616, 734, 750, 854, 899, 1151
HpyF10VI GCNNNNNNNGC 3 cut(s) 337, 346, 848
HpyF3I CTNAG 4 cut(s) 651, 963, 1091, 1170
HpySE526I ACGT 4 cut(s) 99, 536, 777, 1050
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 6 cut(s) 190, 388, 799, 1072, 1133, 1365
HspAI GCGC 1 cut(s) 355
Kzo9I GATC 4 cut(s) 673, 1018, 1096, 1246
LmnI GCTCC 4 cut(s) 177, 337, 868, 1200
Lsp1109I GCAGC 3 cut(s) 267, 838, 866
LweI GCATC 3 cut(s) 228, 838, 851
MabI ACCWGGT 1 cut(s) 694
MaeI CTAG 2 cut(s) 555, 1187
MaeII ACGT 4 cut(s) 99, 536, 777, 1050
MaeIII GTNAC 4 cut(s) 698, 1011, 1046, 1189
MalI GATC 4 cut(s) 675, 1020, 1098, 1248
MboI GATC 4 cut(s) 673, 1018, 1096, 1246
MfeI CAATTG 1 cut(s) 1115
MflI RGATCY 1 cut(s) 1096
MhlI GDGCHC 3 cut(s) 342, 505, 1058
MlyI GAGTC 2 cut(s) 574, 968
MmeI TCCRAC 1 cut(s) 423
Mph1103I ATGCAT 1 cut(s) 752
MroXI GAANNNNTTC 2 cut(s) 750, 803
MseI TTAA 4 cut(s) 428, 740, 1179, 1314
MspR9I CCNGG 5 cut(s) 60, 515, 696, 782, 880
MunI CAATTG 1 cut(s) 1115
Mva1269I GAATGC 2 cut(s) 750, 752
MvaI CCWGG 5 cut(s) 60, 515, 696, 782, 880
MvnI CGCG 5 cut(s) 93, 355, 357, 396, 637
MwoI GCNNNNNNNGC 3 cut(s) 337, 346, 848
NdeII GATC 4 cut(s) 673, 1018, 1096, 1246
NheI GCTAGC 1 cut(s) 554
NlaIII CATG 6 cut(s) 190, 388, 799, 1072, 1133, 1365
NlaIV GGNNCC 3 cut(s) 173, 227, 1098
NruI TCGCGA 1 cut(s) 637
NsiI ATGCAT 1 cut(s) 752
NspV TTCGAA 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 2 cut(s) 750, 752
PdmI GAANNNNTTC 2 cut(s) 750, 803
PfeI GAWTC 4 cut(s) 476, 785, 1074, 1357
PflFI GACNNNGTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 3 cut(s) 257, 853, 856
PleI GAGTC 2 cut(s) 574, 967
PpsI GAGTC 2 cut(s) 574, 967
Ppu21I YACGTR 1 cut(s) 1051
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 5 cut(s) 58, 513, 694, 780, 878
PspGI CCWGG 5 cut(s) 58, 513, 694, 780, 878
PspN4I GGNNCC 3 cut(s) 173, 227, 1098
PspPI GGNCC 2 cut(s) 26, 56
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 955
PsuI RGATCY 1 cut(s) 1096
PsyI GACNNNGTC 1 cut(s) 128
RruI TCGCGA 1 cut(s) 637
RsaI GTAC 3 cut(s) 656, 776, 1185
RsaNI GTAC 3 cut(s) 655, 775, 1184
SacI GAGCTC 1 cut(s) 342
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 4 cut(s) 428, 740, 1179, 1314
SatI GCNGC 3 cut(s) 256, 852, 855
Sau3AI GATC 4 cut(s) 673, 1018, 1096, 1246
Sau96I GGNCC 2 cut(s) 26, 56
ScaI AGTACT 1 cut(s) 656
SchI GAGTC 2 cut(s) 574, 968
ScrFI CCNGG 5 cut(s) 60, 515, 696, 782, 880
SduI GDGCHC 3 cut(s) 342, 505, 1058
SexAI ACCWGGT 1 cut(s) 694
SfaNI GCATC 3 cut(s) 228, 838, 851
SfcI CTRYAG 1 cut(s) 852
SfuI TTCGAA 1 cut(s) 563
SgrDI CGTCGACG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SpeI ACTAGT 1 cut(s) 1186
SsiI CCGC 4 cut(s) 91, 153, 394, 541
SspMI CTAG 2 cut(s) 555, 1187
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 5 cut(s) 58, 513, 694, 780, 878
StyI CCWWGG 3 cut(s) 318, 574, 1305
TaaI ACNGT 2 cut(s) 710, 895
TaiI ACGT 4 cut(s) 102, 539, 780, 1053
TaqI TCGA 2 cut(s) 102, 563
TatI WGTACW 2 cut(s) 654, 1183
TfiI GAWTC 4 cut(s) 476, 785, 1074, 1357
Tru1I TTAA 4 cut(s) 428, 740, 1179, 1314
Tru9I TTAA 4 cut(s) 428, 740, 1179, 1314
TscAI CASTG 1 cut(s) 84
TseI GCWGC 3 cut(s) 255, 851, 854
TspDTI ATGAA 7 cut(s) 279, 311, 812, 1097, 1118, 1170, 1295
TspGWI ACGGA 3 cut(s) 99, 218, 1202
TspRI CASTG 1 cut(s) 84
Tth111I GACNNNGTC 1 cut(s) 128
VneI GTGCAC 1 cut(s) 501
XapI RAATTY 4 cut(s) 518, 610, 829, 1319
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 2 cut(s) 750, 803
XspI CTAG 2 cut(s) 555, 1187
ZraI GACGTC 1 cut(s) 100
ZrmI AGTACT 1 cut(s) 656
Zsp2I ATGCAT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.