MD09G1120500.v1.1

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004058
Physical Location & Seq
Forward (+)
9325440 .. 9327767
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1120500.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCGAAAAAGTTGACAATCAAAGGTCTTTTTAAGCCTAAGGCGAGAACGCCGATAGAGCTTGTAAAGCACATGCGTGAGCTTCTAATATTCGTTGATCAAAATAATGATGTGCGCGAGCAAAAACGCAAAGAAAAGATGGAAGAATTGACAAAAGCAATATTGGAGATAAGAACTGTTGTATATGGAGATGATGATTCAGAACCAAATAAAGATTCTTGTGCACAACTAACACAAGAGTTTTTTAGGGAGAACACTTTCAGGCTCCTCATTGTTTGTCTTTCAAAGCTCAACTTGGGGGATCGTAAAAACGCTACCCATGTCATTGCAAATTTACAAAGGCAAAAGGTTCAGTCGCGTTCGATCGCGTTAGAGTACTTGGAGAATAATATTGATATTATGGACATTCTGATAAAAGGCTATGAAGACAGTGGTGATATTGCTCTATCTTATGGTGCAATTTCAAGGGAATGCATTCGTCATCAGAATATTGCAAGGTATGTCCTCGAATCAGACCACATGAAGAAGTTTTTTTACTACATTCAAACTCCAAATTTCGACATAGCGTCGGATGCTGCGGCTACTTTCAAGGAGCTCATGACTAGGCACAAATCAACTGTTGCTGAATTCCTTTCTGAAAACTATGAGTGGTTTTTCCAAGAGTACAATTCCCAGTTGTTGGAGTCTCCCAATTATATCACCAAACGCCAGGCCATCAAGTTGTTAGGAGATATGTTATTGGATCGCTCAAACTCCGATGTGATGGTTCGATATGTGTGCTCTCTGGATAACATGATAATCCTAATGAACCTTCTCAGGGATCCAAAGAAGACAATCAAGTTAGAAACCTTTCAGGTCTTCAAGTTATTTGTTGCAAACCAAAATAAGCCTCCTGAGATTGTTAATGTACTTGTCACAAATAGAACCAAGCTTCTTCGTTTCTTTAACGACTTCACCATTGAGAAAGGTAGGGTTTCTTTGTTTCGATTCAAAATTTACTCCTTGTCGTTCGTCTTTCTCCTTCGAACTCATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

346

Amino Acids

40.56

Weight (kDa)

9.11

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 10 - 322 8.7e-115 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 679
AccII CGCG 3 cut(s) 117, 358, 368
AciI CCGC 1 cut(s) 578
AclWI GGATC 4 cut(s) 309, 750, 815, 828
AcsI RAATTY 4 cut(s) 331, 553, 626, 993
AfaI GTAC 3 cut(s) 377, 665, 909
AfiI CCNNNNNNNGG 2 cut(s) 679, 817
AgsI TTSAA 6 cut(s) 285, 465, 545, 589, 862, 991
AhdI GACNNNNNGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 708
AjuI GAANNNNNNNTTGG 2 cut(s) 199, 231
AleI CACNNNNGTG 1 cut(s) 75
AluBI AGCT 5 cut(s) 61, 82, 289, 595, 931
AluI AGCT 5 cut(s) 61, 82, 289, 595, 931
Alw21I GWGCWC 3 cut(s) 226, 597, 782
Alw26I GTCTC 1 cut(s) 690
Alw44I GTGCAC 1 cut(s) 222
AlwI GGATC 4 cut(s) 309, 750, 815, 828
AoxI GGCC 1 cut(s) 711
ApaLI GTGCAC 1 cut(s) 222
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 4 cut(s) 331, 553, 626, 993
Asp700I GAANNNNTTC 3 cut(s) 257, 474, 849
AspLEI GCGC 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 446, 691, 946
AsuII TTCGAA 1 cut(s) 1024
AxyI CCTNAGG 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 226
BamHI GGATCC 1 cut(s) 820
BanII GRGCYC 1 cut(s) 597
BarI GAAGNNNNNNTAC 2 cut(s) 518, 550
BbsI GAAGAC 3 cut(s) 432, 836, 850
Bbv12I GWGCWC 3 cut(s) 226, 597, 782
BbvI GCAGC 1 cut(s) 562
BccI CCATC 3 cut(s) 133, 722, 757
BcgI CGANNNNNNTGC 2 cut(s) 759, 793
BciT130I CCWGG 1 cut(s) 710
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 1 cut(s) 603
BisI GCNGC 2 cut(s) 576, 579
BlsI GCNGC 2 cut(s) 577, 580
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 710
BmeRI GACNNNNNGTC 1 cut(s) 565
BmiI GGNNCC 2 cut(s) 266, 822
BmrFI CCNGG 1 cut(s) 710
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 1 cut(s) 562
BmuI ACTGGG 1 cut(s) 667
BpiI GAAGAC 3 cut(s) 432, 836, 850
Bpu14I TTCGAA 1 cut(s) 1024
Bsc4I CCNNNNNNNGG 2 cut(s) 679, 817
Bse1I ACTGG 1 cut(s) 673
Bse21I CCTNAGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 710
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 2 cut(s) 679, 817
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 2 cut(s) 829, 885
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 1 cut(s) 257
BseSI GKGCMC 1 cut(s) 226
BseXI GCAGC 1 cut(s) 562
Bsh1236I CGCG 3 cut(s) 117, 358, 368
Bsh1285I CGRYCG 1 cut(s) 366
BshFI GGCC 1 cut(s) 713
BsiEI CGRYCG 1 cut(s) 366
BsiHKAI GWGCWC 3 cut(s) 226, 597, 782
BslI CCNNNNNNNGG 2 cut(s) 679, 817
BsmAI GTCTC 1 cut(s) 690
BsmI GAATGC 2 cut(s) 474, 476
BsnI GGCC 1 cut(s) 713
Bsp119I TTCGAA 1 cut(s) 1024
Bsp1286I GDGCHC 3 cut(s) 226, 597, 782
Bsp143I GATC 5 cut(s) 97, 301, 363, 742, 820
BspACI CCGC 1 cut(s) 578
BspANI GGCC 1 cut(s) 713
BspCNI CTCAG 2 cut(s) 828, 886
BspFNI CGCG 3 cut(s) 117, 358, 368
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 266, 822
BspPI GGATC 4 cut(s) 309, 750, 815, 828
BspT104I TTCGAA 1 cut(s) 1024
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 1 cut(s) 673
BssMI GATC 5 cut(s) 97, 301, 363, 742, 820
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 3 cut(s) 178, 431, 619
BstBI TTCGAA 1 cut(s) 1024
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 39, 815, 894
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 3 cut(s) 117, 358, 368
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 5 cut(s) 100, 304, 366, 745, 823
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 5 cut(s) 97, 301, 363, 742, 820
BstMCI CGRYCG 1 cut(s) 366
BstMWI GCNNNNNNNGC 3 cut(s) 58, 67, 572
BstNI CCWGG 1 cut(s) 710
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 708
BstSLI GKGCMC 1 cut(s) 226
BstUI CGCG 3 cut(s) 117, 358, 368
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 3 cut(s) 432, 836, 850
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
Bsu36I CCTNAGG 1 cut(s) 39
BsuRI GGCC 1 cut(s) 713
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 1 cut(s) 119
CciI TCATGA 1 cut(s) 597
CfoI GCGC 1 cut(s) 117
CseI GACGC 1 cut(s) 555
Csp6I GTAC 3 cut(s) 376, 664, 908
CviAII CATG 5 cut(s) 73, 320, 520, 598, 793
CviQI GTAC 3 cut(s) 376, 664, 908
DdeI CTNAG 3 cut(s) 39, 815, 894
DpnI GATC 5 cut(s) 99, 303, 365, 744, 822
DpnII GATC 5 cut(s) 97, 301, 363, 742, 820
DriI GACNNNNNGTC 1 cut(s) 565
Eam1105I GACNNNNNGTC 1 cut(s) 565
Ecl136II GAGCTC 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 597
Eco53kI GAGCTC 1 cut(s) 595
Eco81I CCTNAGG 1 cut(s) 39
EcoICRI GAGCTC 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 1 cut(s) 708
EcoT22I ATGCAT 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 597
FaeI CATG 5 cut(s) 76, 323, 523, 601, 796
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FatI CATG 5 cut(s) 72, 319, 519, 597, 792
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 576, 579
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 2 cut(s) 576, 579
FspBI CTAG 1 cut(s) 603
GlaI GCGC 1 cut(s) 116
GluI GCNGC 2 cut(s) 576, 579
HaeIII GGCC 1 cut(s) 713
HgaI GACGC 1 cut(s) 555
HhaI GCGC 1 cut(s) 117
Hin1II CATG 5 cut(s) 76, 323, 523, 601, 796
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 5 cut(s) 197, 215, 509, 683, 987
HphI GGTGA 3 cut(s) 446, 691, 946
Hpy166II GTNNAC 2 cut(s) 15, 224
Hpy188I TCNGA 7 cut(s) 202, 411, 486, 514, 571, 637, 757
Hpy188III TCNNGA 3 cut(s) 598, 785, 893
Hpy8I GTNNAC 2 cut(s) 15, 224
Hpy99I CGWCG 1 cut(s) 571
HpyAV CCTTC 2 cut(s) 821, 1031
HpyCH4III ACNGT 3 cut(s) 178, 431, 619
HpyCH4V TGCA 6 cut(s) 224, 329, 458, 474, 494, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 67, 572
HpyF3I CTNAG 3 cut(s) 39, 815, 894
Hsp92II CATG 5 cut(s) 76, 323, 523, 601, 796
HspAI GCGC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 5 cut(s) 97, 301, 363, 742, 820
LmnI GCTCC 2 cut(s) 270, 592
LpnPI CCDG 8 cut(s) 247, 686, 695, 722, 770, 802, 839, 906
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 1 cut(s) 562
MaeI CTAG 1 cut(s) 603
MaeIII GTNAC 1 cut(s) 913
MalI GATC 5 cut(s) 99, 303, 365, 744, 822
MboI GATC 5 cut(s) 97, 301, 363, 742, 820
MboII GAAGA 6 cut(s) 155, 437, 535, 841, 850, 926
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 3 cut(s) 226, 597, 782
MluCI AATT 8 cut(s) 146, 331, 459, 553, 626, 667, 691, 993
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 2 cut(s) 549, 660
MnlI CCTC 3 cut(s) 278, 515, 900
Mph1103I ATGCAT 1 cut(s) 476
MroXI GAANNNNTTC 3 cut(s) 257, 474, 849
MseI TTAA 4 cut(s) 33, 903, 945, 1036
MslI CAYNNNNRTG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 710
Mva1269I GAATGC 2 cut(s) 474, 476
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 3 cut(s) 117, 358, 368
MwoI GCNNNNNNNGC 3 cut(s) 58, 67, 572
NdeII GATC 5 cut(s) 97, 301, 363, 742, 820
NlaIII CATG 5 cut(s) 76, 323, 523, 601, 796
NlaIV GGNNCC 2 cut(s) 266, 822
NmuCI GTSAC 1 cut(s) 913
NsiI ATGCAT 1 cut(s) 476
NspI RCATGY 1 cut(s) 76
NspV TTCGAA 1 cut(s) 1024
OliI CACNNNNGTG 1 cut(s) 75
PagI TCATGA 1 cut(s) 597
PctI GAATGC 2 cut(s) 474, 476
PdmI GAANNNNTTC 3 cut(s) 257, 474, 849
PfeI GAWTC 4 cut(s) 197, 215, 509, 987
PflMI CCANNNNNTGG 1 cut(s) 679
PkrI GCNGC 2 cut(s) 577, 580
Ple19I CGATCG 1 cut(s) 366
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
Psp124BI GAGCTC 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 708
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 2 cut(s) 266, 822
PsuI RGATCY 1 cut(s) 820
PvuI CGATCG 1 cut(s) 366
RsaI GTAC 3 cut(s) 377, 665, 909
RsaNI GTAC 3 cut(s) 376, 664, 908
RseI CAYNNNNRTG 1 cut(s) 75
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 4 cut(s) 33, 903, 945, 1036
SatI GCNGC 2 cut(s) 576, 579
Sau3AI GATC 5 cut(s) 97, 301, 363, 742, 820
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 1 cut(s) 710
SduI GDGCHC 3 cut(s) 226, 597, 782
SfaNI GCATC 1 cut(s) 562
SfuI TTCGAA 1 cut(s) 1024
SmiMI CAYNNNNRTG 1 cut(s) 75
Sse9I AATT 8 cut(s) 146, 331, 459, 553, 626, 667, 691, 993
SsiI CCGC 1 cut(s) 578
SspI AATATT 4 cut(s) 90, 162, 391, 490
SspMI CTAG 1 cut(s) 603
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 1 cut(s) 708
TaaI ACNGT 3 cut(s) 178, 431, 619
TaqI TCGA 6 cut(s) 362, 507, 558, 769, 985, 1024
TasI AATT 8 cut(s) 146, 331, 459, 553, 626, 667, 691, 993
TatI WGTACW 3 cut(s) 375, 663, 907
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 4 cut(s) 197, 215, 509, 987
Tru1I TTAA 4 cut(s) 33, 903, 945, 1036
Tru9I TTAA 4 cut(s) 33, 903, 945, 1036
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 3 cut(s) 438, 536, 821
TspRI CASTG 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 679
VneI GTGCAC 1 cut(s) 222
XapI RAATTY 4 cut(s) 331, 553, 626, 993
XceI RCATGY 1 cut(s) 76
XmnI GAANNNNTTC 3 cut(s) 257, 474, 849
XspI CTAG 1 cut(s) 603
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.