RchiOBHm_Chr2g0156881

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004058
Physical Location & Seq
Reverse (-)
73397036 .. 73399746
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52564

Sequence Viewer

Length: 1200 bp
ATGGATTCTGAAGCTCTATCTCAATCCCATATGTTGTTCCCATCTCTTCTCATAAACCCTAAATTGAGGTTCCGTCCCCAGAAACGCAGCAAATATGAAAGGTCTCTTTTGATAGTGAAATTGAAGATGAAGGGTCTTCTCAAGAAATCCAAGGCACGCAAGCCTTTGGAACTCGTCAAGCACACGCGCGAGCTTCTCATCTTCTTCAATGGCAACATGGAAGTCCGCGAGCAGAAACGCAAAGACAAGATGGAAGAATTAACAAAATCAATGTTGGAGATAAGAACACTTCTGTATGGCGATGGTGAATCAGAGCCGAATAAAGACACTTGTGCACAGCTTACCCAGGAATTTTTCAAAGATGATACGTTCCGCCTTCTCATTGCTAGCCTTTCGAAGCTCAACCTTGAGACTCGTAAAAACGCAACACATATTGTCGCAAATTTGCAAAGGCAACAGGTTCAATCGCGATTGATTGCTTCTGAGTATTTGGAAAAGAATATTGATCTTATAGATATTCTGATACCTGGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTCGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGGCAGGCTATCAAGCTGTTAGGAAATATGTTACTGGATCGCTCAAACTCTGCTGTGATGGTTCGATACGTGTGCTCTTTGGATAACATGAGAATCCTAATGAACCTTCTCAGGGATCCAAACAAGACAATCCAATTGGAGACCTTTCATGTCTTCAAGCTATTTGTTGCAAATGAAAATAAGCCTCCTCAGATTGTTAATGTACTAGTAACAAATAGGAGCAAGCTTCTCCGTTTCTTTGGCAACTTCACCATTGAAAAAGAGGATCAGCAGTTTGAAGCAGACAAAGTTGAACTTATGAAAGAGATTTCTATTCTTGAACCCAAGGATATTAAGGAATTTTCGTCAATCGTGAGCTTTAGAGAGAATTGTGAGATTCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

399

Amino Acids

46.66

Weight (kDa)

9.03

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 47 - 377 6.5e-124 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 187, 189, 228, 469
AciI CCGC 2 cut(s) 226, 373
AclWI GGATC 4 cut(s) 858, 923, 936, 1086
AcsI RAATTY 4 cut(s) 350, 442, 661, 1151
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 2 cut(s) 608, 1017
AfiI CCNNNNNNNGG 2 cut(s) 533, 925
AflIII ACRYGT 1 cut(s) 882
AhlI ACTAGT 1 cut(s) 1018
AjnI CCWGG 4 cut(s) 345, 526, 612, 710
AluBI AGCT 9 cut(s) 14, 193, 340, 400, 703, 829, 973, 1039, 1170
AluI AGCT 9 cut(s) 14, 193, 340, 400, 703, 829, 973, 1039, 1170
Alw21I GWGCWC 2 cut(s) 337, 890
Alw26I GTCTC 5 cut(s) 108, 404, 531, 798, 947
Alw44I GTGCAC 1 cut(s) 333
AlwI GGATC 4 cut(s) 858, 923, 936, 1086
AlwNI CAGNNNCTG 1 cut(s) 787
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 3 cut(s) 87, 683, 686
ApoI RAATTY 4 cut(s) 350, 442, 661, 1151
Asp700I GAANNNNTTC 3 cut(s) 582, 635, 638
AspLEI GCGC 1 cut(s) 189
AsuHPI GGTGA 4 cut(s) 317, 554, 799, 1054
AsuII TTCGAA 1 cut(s) 395
AsuNHI GCTAGC 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 337
BamHI GGATCC 1 cut(s) 928
BbsI GAAGAC 2 cut(s) 128, 958
Bbv12I GWGCWC 2 cut(s) 337, 890
BbvI GCAGC 3 cut(s) 99, 670, 698
BccI CCATC 4 cut(s) 49, 244, 296, 865
BceAI ACGGC 1 cut(s) 830
BcgI CGANNNNNNTGC 2 cut(s) 867, 901
BciT130I CCWGG 4 cut(s) 347, 528, 614, 712
BcoDI GTCTC 5 cut(s) 108, 404, 531, 798, 947
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 2 cut(s) 387, 1019
BfmI CTRYAG 1 cut(s) 684
BisI GCNGC 3 cut(s) 88, 684, 687
BlsI GCNGC 3 cut(s) 89, 685, 688
Bme1390I CCNGG 4 cut(s) 347, 528, 614, 712
BmiI GGNNCC 2 cut(s) 71, 930
BmrFI CCNGG 4 cut(s) 347, 528, 614, 712
BmsI GCATC 2 cut(s) 670, 683
BmtI GCTAGC 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 128, 958
BpmI CTGGAG 1 cut(s) 809
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 125, 428
BsaAI YACGTR 1 cut(s) 883
BsaI GGTCTC 2 cut(s) 108, 947
BsaJI CCNNGG 3 cut(s) 150, 345, 1137
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 925
Bse1I ACTGG 2 cut(s) 761, 852
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 4 cut(s) 347, 528, 614, 712
BseDI CCNNGG 3 cut(s) 150, 345, 1137
BseGI GGATG 1 cut(s) 685
BseLI CCNNNNNNNGG 2 cut(s) 533, 925
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 4 cut(s) 474, 809, 937, 1016
BseNI ACTGG 2 cut(s) 761, 852
BseRI GAGGAG 1 cut(s) 990
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 3 cut(s) 99, 670, 698
Bsh1236I CGCG 4 cut(s) 187, 189, 228, 469
BsiHKAI GWGCWC 2 cut(s) 337, 890
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 533, 925
BsmAI GTCTC 5 cut(s) 108, 404, 531, 798, 947
BsmBI CGTCTC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 60
BsmI GAATGC 2 cut(s) 582, 584
Bso31I GGTCTC 2 cut(s) 108, 947
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 337, 890
Bsp143I GATC 4 cut(s) 505, 850, 928, 1078
Bsp68I TCGCGA 1 cut(s) 469
BspACI CCGC 2 cut(s) 226, 373
BspCNI CTCAG 4 cut(s) 475, 808, 936, 1015
BspFNI CGCG 4 cut(s) 187, 189, 228, 469
BspLI GGNNCC 2 cut(s) 71, 930
BspMAI CTGCAG 1 cut(s) 688
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 4 cut(s) 858, 923, 936, 1086
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 2 cut(s) 108, 947
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 2 cut(s) 761, 852
BssECI CCNNGG 3 cut(s) 150, 345, 1137
BssMI GATC 4 cut(s) 505, 850, 928, 1078
BssT1I CCWWGG 2 cut(s) 150, 1137
Bst2UI CCWGG 4 cut(s) 347, 528, 614, 712
Bst4CI ACNGT 2 cut(s) 542, 727
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 680
BstBAI YACGTR 1 cut(s) 883
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 7 cut(s) 157, 161, 191, 230, 388, 819, 1037
BstDEI CTNAG 4 cut(s) 483, 795, 923, 1002
BstF5I GGATG 1 cut(s) 685
BstFNI CGCG 4 cut(s) 187, 189, 228, 469
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 4 cut(s) 508, 853, 931, 1081
BstMAI GTCTC 5 cut(s) 108, 404, 531, 798, 947
BstMBI GATC 4 cut(s) 505, 850, 928, 1078
BstMWI GCNNNNNNNGC 1 cut(s) 680
BstNI CCWGG 4 cut(s) 347, 528, 614, 712
BstSCI CCNGG 4 cut(s) 345, 526, 612, 710
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 4 cut(s) 187, 189, 228, 469
BstV1I GCAGC 3 cut(s) 99, 670, 698
BstV2I GAAGAC 2 cut(s) 128, 958
BstX2I RGATCY 1 cut(s) 928
BstYI RGATCY 1 cut(s) 928
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 685
BtuMI TCGCGA 1 cut(s) 469
Cac8I GCNNGC 7 cut(s) 157, 161, 191, 230, 388, 819, 1037
CaiI CAGNNNCTG 1 cut(s) 787
CfoI GCGC 1 cut(s) 189
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 2 cut(s) 607, 1016
CviAII CATG 5 cut(s) 217, 628, 901, 962, 1194
CviQI GTAC 2 cut(s) 607, 1016
DdeI CTNAG 4 cut(s) 483, 795, 923, 1002
DpnI GATC 4 cut(s) 507, 852, 930, 1080
DpnII GATC 4 cut(s) 505, 850, 928, 1078
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 150, 1137
Eco31I GGTCTC 2 cut(s) 108, 947
Eco57I CTGAAG 1 cut(s) 30
EcoRII CCWGG 4 cut(s) 345, 526, 612, 710
EcoT14I CCWWGG 2 cut(s) 150, 1137
EcoT22I ATGCAT 1 cut(s) 584
ErhI CCWWGG 2 cut(s) 150, 1137
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 5 cut(s) 220, 631, 904, 965, 1197
FalI AAGNNNNNCTT 4 cut(s) 623, 655, 1092, 1124
FaqI GGGAC 1 cut(s) 60
FatI CATG 5 cut(s) 216, 627, 900, 961, 1193
FauNDI CATATG 1 cut(s) 30
Fnu4HI GCNGC 3 cut(s) 88, 684, 687
FokI GGATG 1 cut(s) 692
Fsp4HI GCNGC 3 cut(s) 88, 684, 687
FspBI CTAG 2 cut(s) 387, 1019
GlaI GCGC 1 cut(s) 188
GluI GCNGC 3 cut(s) 88, 684, 687
GsuI CTGGAG 1 cut(s) 809
HhaI GCGC 1 cut(s) 189
Hin1II CATG 5 cut(s) 220, 631, 904, 965, 1197
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HindIII AAGCTT 1 cut(s) 1037
HinfI GANTC 7 cut(s) 5, 308, 412, 617, 791, 906, 1189
HphI GGTGA 4 cut(s) 317, 554, 799, 1054
Hpy166II GTNNAC 2 cut(s) 335, 723
Hpy188I TCNGA 8 cut(s) 10, 313, 484, 522, 622, 679, 798, 1005
Hpy188III TCNNGA 4 cut(s) 142, 468, 1130, 1165
Hpy8I GTNNAC 2 cut(s) 335, 723
HpyAV CCTTC 3 cut(s) 124, 386, 929
HpyCH4III ACNGT 2 cut(s) 542, 727
HpyCH4IV ACGT 3 cut(s) 368, 609, 882
HpyCH4V TGCA 7 cut(s) 335, 448, 566, 582, 686, 731, 983
HpyF10VI GCNNNNNNNGC 1 cut(s) 680
HpyF3I CTNAG 4 cut(s) 483, 795, 923, 1002
HpySE526I ACGT 3 cut(s) 368, 609, 882
Hsp92II CATG 5 cut(s) 220, 631, 904, 965, 1197
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 4 cut(s) 505, 850, 928, 1078
LmnI GCTCC 2 cut(s) 700, 1032
Lsp1109I GCAGC 3 cut(s) 99, 670, 698
LweI GCATC 2 cut(s) 670, 683
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 2 cut(s) 387, 1019
MaeII ACGT 3 cut(s) 368, 609, 882
MaeIII GTNAC 2 cut(s) 843, 1021
MalI GATC 4 cut(s) 507, 852, 930, 1080
MboI GATC 4 cut(s) 505, 850, 928, 1078
MboII GAAGA 9 cut(s) 38, 128, 136, 193, 196, 266, 631, 643, 958
MfeI CAATTG 1 cut(s) 947
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 2 cut(s) 337, 890
MlyI GAGTC 2 cut(s) 406, 800
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 5 cut(s) 60, 597, 1008, 1011, 1069
Mph1103I ATGCAT 1 cut(s) 584
MroXI GAANNNNTTC 3 cut(s) 582, 635, 638
MseI TTAA 4 cut(s) 260, 572, 1011, 1146
MspR9I CCNGG 4 cut(s) 347, 528, 614, 712
MunI CAATTG 1 cut(s) 947
Mva1269I GAATGC 2 cut(s) 582, 584
MvaI CCWGG 4 cut(s) 347, 528, 614, 712
MvnI CGCG 4 cut(s) 187, 189, 228, 469
MwoI GCNNNNNNNGC 1 cut(s) 680
NdeI CATATG 1 cut(s) 30
NdeII GATC 4 cut(s) 505, 850, 928, 1078
NheI GCTAGC 1 cut(s) 386
NlaIII CATG 5 cut(s) 220, 631, 904, 965, 1197
NlaIV GGNNCC 2 cut(s) 71, 930
NruI TCGCGA 1 cut(s) 469
NsiI ATGCAT 1 cut(s) 584
NspV TTCGAA 1 cut(s) 395
PctI GAATGC 2 cut(s) 582, 584
PdmI GAANNNNTTC 3 cut(s) 582, 635, 638
PfeI GAWTC 5 cut(s) 5, 308, 617, 906, 1189
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 3 cut(s) 89, 685, 688
PleI GAGTC 2 cut(s) 406, 799
PpsI GAGTC 2 cut(s) 406, 799
Ppu21I YACGTR 1 cut(s) 883
Psp6I CCWGG 4 cut(s) 345, 526, 612, 710
PspGI CCWGG 4 cut(s) 345, 526, 612, 710
PspN4I GGNNCC 2 cut(s) 71, 930
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 928
RruI TCGCGA 1 cut(s) 469
RsaI GTAC 2 cut(s) 608, 1017
RsaNI GTAC 2 cut(s) 607, 1016
SaqAI TTAA 4 cut(s) 260, 572, 1011, 1146
SatI GCNGC 3 cut(s) 88, 684, 687
Sau3AI GATC 4 cut(s) 505, 850, 928, 1078
SchI GAGTC 2 cut(s) 406, 800
ScrFI CCNGG 4 cut(s) 347, 528, 614, 712
SduI GDGCHC 2 cut(s) 337, 890
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 2 cut(s) 670, 683
SfcI CTRYAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 140, 407
SmoI CTYRAG 2 cut(s) 140, 407
SpeI ACTAGT 1 cut(s) 1018
SsiI CCGC 2 cut(s) 226, 373
SspI AATATT 1 cut(s) 502
SspMI CTAG 2 cut(s) 387, 1019
StyD4I CCNGG 4 cut(s) 345, 526, 612, 710
StyI CCWWGG 2 cut(s) 150, 1137
TaaI ACNGT 2 cut(s) 542, 727
TaiI ACGT 3 cut(s) 371, 612, 885
TaqI TCGA 3 cut(s) 395, 642, 877
TatI WGTACW 1 cut(s) 1015
TfiI GAWTC 5 cut(s) 5, 308, 617, 906, 1189
Tru1I TTAA 4 cut(s) 260, 572, 1011, 1146
Tru9I TTAA 4 cut(s) 260, 572, 1011, 1146
TseI GCWGC 3 cut(s) 87, 683, 686
TspDTI ATGAA 7 cut(s) 111, 143, 644, 929, 950, 1002, 1127
TspGWI ACGGA 2 cut(s) 62, 1034
VneI GTGCAC 1 cut(s) 333
XapI RAATTY 4 cut(s) 350, 442, 661, 1151
XmnI GAANNNNTTC 3 cut(s) 582, 635, 638
XspI CTAG 2 cut(s) 387, 1019
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.