FvH4_7g10070

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004245
Physical Location & Seq
Forward (+)
9510991 .. 9512147
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g10070.t1

Sequence Viewer

Length: 717 bp
ATGTCCATGGAAACTGAAAGCTTCTCCAACTCAGACTGGGCTCTCTTGGAGTCGATCCGCCAGCATCTTCTTGACGACGATTTCGATACCCAGTCGGCATTTTTTGCAGACTCTCCTTCTCAGAGTACTTCCAGCTCAAACTGCACTGAGATATCGAGCAGTAATGTAGACATAGTGGAGCAGAACAATGCATTCACTACTGAACTTGACGTGGCGGCGCGTGATGACCATGCGCCATCGGCACCGAACCATTTTAGGGGAGTGAGGAGAAGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAAAAGAACGGGACGAGAGTGTGGCTCGGAACCTACGAGACGGCGGAGGACGCAGGTTTAGCTTACGATCGAGCAGCTTTCAGAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCACGCATGATATGGAGGTGGGTCGTGTGCTTCCCAAGCGTGCCTCGCCGGAGCCGTCATCTCCGTCGTCCACCATGTCTTCCGAAGATGGCTCGCCGAAGCCTAAGAGGATGAGGAGGAGTGGAGGGGACTCCGCCGCGGCAAAAGCCAAGTTGGATGAAGCTGCCGGAGAGGTTCATGTGTTCCAAGTGGACTCATTCACATTTGGCGACGAATTATTGGTTGAAGATATGGTGCTTTTGTCGCCGTCTGAATGCTCCGACATTCTTTCTTTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

239

Amino Acids

26.16

Weight (kDa)

4.95

Isoelectric Point (pI)

64.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 84 - 135 2.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 241
AccI GTMKAC 1 cut(s) 168
AccII CGCG 3 cut(s) 220, 290, 575
AciI CCGC 8 cut(s) 58, 215, 290, 293, 353, 570, 573, 575
AclWI GGATC 1 cut(s) 49
AfaI GTAC 2 cut(s) 127, 287
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 1 cut(s) 662
AjiI CACGTC 1 cut(s) 211
AluBI AGCT 7 cut(s) 21, 135, 371, 386, 410, 415, 599
AluI AGCT 7 cut(s) 21, 135, 371, 386, 410, 415, 599
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 1 cut(s) 49
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 383, 599
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 2 cut(s) 220, 235
AspS9I GGNCC 1 cut(s) 304
AvaII GGWCC 1 cut(s) 304
BanI GGYRCC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 395, 586
BccI CCATC 2 cut(s) 244, 518
BceAI ACGGC 4 cut(s) 259, 366, 475, 667
BcoDI GTCTC 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 353
BisI GCNGC 6 cut(s) 216, 291, 384, 573, 576, 600
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 6 cut(s) 217, 292, 385, 574, 577, 601
BmcAI AGTACT 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 304
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 6 cut(s) 243, 305, 306, 340, 403, 489
BmrI ACTGGG 2 cut(s) 46, 85
BmsI GCATC 1 cut(s) 73
BmuI ACTGGG 2 cut(s) 46, 85
BpiI GAAGAC 1 cut(s) 507
BplI GAGNNNNNCTC 2 cut(s) 318, 350
Bpu1102I GCTNAGC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 6, 275, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 41, 91
BseDI CCNNGG 3 cut(s) 6, 275, 573
BseGI GGATG 2 cut(s) 552, 598
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 3 cut(s) 45, 134, 138
BseNI ACTGG 2 cut(s) 41, 91
BseRI GAGGAG 3 cut(s) 280, 565, 568
BseXI GCAGC 2 cut(s) 395, 586
BsgI GTGCAG 1 cut(s) 127
Bsh1236I CGCG 3 cut(s) 220, 290, 575
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 1 cut(s) 241
BsiEI CGRYCG 1 cut(s) 379
BsiSI CCGG 2 cut(s) 485, 603
BslFI GGGAC 3 cut(s) 317, 334, 578
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 341
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 3 cut(s) 317, 334, 578
BsmI GAATGC 3 cut(s) 191, 399, 695
BsnI GGCC 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 2 cut(s) 54, 376
Bsp1720I GCTNAGC 1 cut(s) 411
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 8 cut(s) 58, 215, 290, 293, 353, 570, 573, 575
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 3 cut(s) 44, 133, 139
BspFNI CGCG 3 cut(s) 220, 290, 575
BspLI GGNNCC 6 cut(s) 243, 305, 306, 340, 403, 489
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 241
BsrI ACTGG 2 cut(s) 41, 91
BssECI CCNNGG 3 cut(s) 6, 275, 573
BssMI GATC 2 cut(s) 54, 376
BssT1I CCWWGG 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 4 cut(s) 62, 440, 477, 530
BstDEI CTNAG 5 cut(s) 31, 120, 147, 411, 540
BstDSI CCRYGG 3 cut(s) 6, 275, 573
BstF5I GGATG 2 cut(s) 552, 598
BstFNI CGCG 3 cut(s) 220, 290, 575
BstHHI GCGC 2 cut(s) 220, 235
BstKTI GATC 2 cut(s) 57, 379
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 2 cut(s) 54, 376
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 9 cut(s) 104, 141, 239, 359, 368, 472, 481, 581, 679
BstUI CGCG 3 cut(s) 220, 290, 575
BstV1I GCAGC 2 cut(s) 395, 586
BstV2I GAAGAC 1 cut(s) 507
BsuRI GGCC 1 cut(s) 274
BtgI CCRYGG 3 cut(s) 6, 275, 573
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 552, 598
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 4 cut(s) 62, 440, 477, 530
CfoI GCGC 2 cut(s) 220, 235
Cfr13I GGNCC 1 cut(s) 304
Cfr42I CCGCGG 1 cut(s) 576
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 126, 286
CviAII CATG 5 cut(s) 7, 230, 443, 511, 614
CviQI GTAC 2 cut(s) 126, 286
DdeI CTNAG 5 cut(s) 31, 120, 147, 411, 540
DpnI GATC 2 cut(s) 56, 378
DpnII GATC 2 cut(s) 54, 376
EciI GGCGGA 4 cut(s) 47, 308, 368, 559
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 304
EcoRV GATATC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 5 cut(s) 10, 233, 446, 514, 617
FaiI YATR 9 cut(s) 8, 173, 231, 444, 449, 512, 615, 668, 715
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 404, 436
FaqI GGGAC 3 cut(s) 317, 334, 578
FatI CATG 5 cut(s) 6, 229, 442, 510, 613
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 6 cut(s) 216, 291, 384, 573, 576, 600
FokI GGATG 2 cut(s) 559, 605
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 6 cut(s) 216, 291, 384, 573, 576, 600
GlaI GCGC 2 cut(s) 219, 234
GluI GCNGC 6 cut(s) 216, 291, 384, 573, 576, 600
HaeIII GGCC 1 cut(s) 274
HapII CCGG 2 cut(s) 485, 603
HgaI GACGC 1 cut(s) 368
HhaI GCGC 2 cut(s) 220, 235
Hin1II CATG 5 cut(s) 10, 233, 446, 514, 617
Hin6I GCGC 2 cut(s) 218, 233
HinP1I GCGC 2 cut(s) 218, 233
HindIII AAGCTT 2 cut(s) 19, 413
HinfI GANTC 4 cut(s) 50, 110, 566, 629
HpaII CCGG 2 cut(s) 485, 603
Hpy166II GTNNAC 3 cut(s) 169, 507, 628
Hpy188I TCNGA 7 cut(s) 34, 123, 338, 392, 520, 688, 697
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 3 cut(s) 169, 507, 628
Hpy99I CGWCG 3 cut(s) 80, 505, 650
HpyAV CCTTC 2 cut(s) 126, 264
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 3 cut(s) 107, 144, 191
HpyF10VI GCNNNNNNNGC 9 cut(s) 104, 141, 239, 359, 368, 472, 481, 581, 679
HpyF3I CTNAG 5 cut(s) 31, 120, 147, 411, 540
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 5 cut(s) 10, 233, 446, 514, 617
HspAI GCGC 2 cut(s) 218, 233
KflI GGGWCCC 1 cut(s) 304
KspI CCGCGG 1 cut(s) 576
Kzo9I GATC 2 cut(s) 54, 376
LmnI GCTCC 4 cut(s) 178, 407, 487, 698
LpnPI CCDG 7 cut(s) 22, 74, 104, 145, 348, 498, 616
Lsp1109I GCAGC 2 cut(s) 395, 586
LweI GCATC 1 cut(s) 73
MaeII ACGT 1 cut(s) 210
MalI GATC 2 cut(s) 56, 378
MboI GATC 2 cut(s) 54, 376
MboII GAAGA 4 cut(s) 59, 507, 533, 674
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 1 cut(s) 650
MlyI GAGTC 4 cut(s) 59, 104, 560, 623
MmeI TCCRAC 2 cut(s) 51, 570
Mph1103I ATGCAT 1 cut(s) 193
MseI TTAA 1 cut(s) 417
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 2 cut(s) 485, 603
Mva1269I GAATGC 3 cut(s) 191, 399, 695
MvnI CGCG 3 cut(s) 220, 290, 575
MwoI GCNNNNNNNGC 9 cut(s) 104, 141, 239, 359, 368, 472, 481, 581, 679
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 54, 376
NlaIII CATG 5 cut(s) 10, 233, 446, 514, 617
NlaIV GGNNCC 6 cut(s) 243, 305, 306, 340, 403, 489
NsiI ATGCAT 1 cut(s) 193
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 342, 488
PctI GAATGC 3 cut(s) 191, 399, 695
PkrI GCNGC 6 cut(s) 217, 292, 385, 574, 577, 601
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 4 cut(s) 58, 104, 560, 623
PpsI GAGTC 4 cut(s) 58, 104, 560, 623
PpuMI RGGWCCY 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 304
PspN4I GGNNCC 6 cut(s) 243, 305, 306, 340, 403, 489
PspPI GGNCC 1 cut(s) 304
PspPPI RGGWCCY 1 cut(s) 304
PvuI CGATCG 1 cut(s) 379
RsaI GTAC 2 cut(s) 127, 287
RsaNI GTAC 2 cut(s) 126, 286
SacII CCGCGG 1 cut(s) 576
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 6 cut(s) 216, 291, 384, 573, 576, 600
Sau3AI GATC 2 cut(s) 54, 376
Sau96I GGNCC 1 cut(s) 304
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 4 cut(s) 59, 104, 560, 623
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 1 cut(s) 73
Sfr303I CCGCGG 1 cut(s) 576
SgrBI CCGCGG 1 cut(s) 576
SinI GGWCC 1 cut(s) 304
Sse9I AATT 1 cut(s) 650
SsiI CCGC 8 cut(s) 58, 215, 290, 293, 353, 570, 573, 575
StyI CCWWGG 1 cut(s) 6
TaiI ACGT 1 cut(s) 213
TaqI TCGA 4 cut(s) 53, 84, 155, 379
TasI AATT 1 cut(s) 650
TatI WGTACW 1 cut(s) 125
TauI GCSGC 4 cut(s) 218, 293, 575, 578
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 151
TseI GCWGC 2 cut(s) 383, 599
TspDTI ATGAA 2 cut(s) 602, 609
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 168
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.