MD07G1106300.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004245
Physical Location & Seq
Forward (+)
12047989 .. 12048651
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1106300.v1.1.491

Sequence Viewer

Length: 663 bp
ATGTCGACAGAAAGCATGTCAAGATCCAACTGGGCTCTCTTGGAATCGATTCGTCAGCATCTACTACACGATGATTTCGAAACCCAGGAGACATTTTTGGCCGATGTAGCTGAGCTTGATGCACCATCGCTTAATTCTCCAAGTACTTCAAGCTCTAGCAATGCTCATTTTCTTGTCGAGGATTTGGGTGACACAATGGGAATAATGAACCATGCTTTGGACACATCAAATAACTTCGAGGTGGCAGCGTGTGTTGTTCAAGTCCCACAGAGCGGACGGAATTTTAGGGGAGTGAGGCAACGACCCTGGGGTAAGTACGCAGCTGAGATAAGAGACCCAAAGAAGAACGGGGCCAGAATCTGGCTCGGAACCTACGAGACAGCTGTGGATGCCGGCTTGGCTTATGACCGAGCCGCTTTTAAAATGCGTGGCTCCAAGGCTAAGCTTAATTTTCCTCACCTCATTGGCACGCATGATTCGGAGCCGGGTCGTGTGGCTTCTAATCGTTGCTCACTGAAGCCATCATCTTCGTCTGTATTATCGTCGGATGGTGGAGGATCACCAACGCTTAAGCGGAAAAGGAATGTAGTTTGCTCGGCGGAAAAAGCCAAGTTGGAAGAAGGAGACGAACCATTTCAAGTGTTCCAACTGAACTCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

221

Amino Acids

24.11

Weight (kDa)

6.31

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 94 - 145 5.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 217, 360
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 1 cut(s) 5
AciI CCGC 4 cut(s) 273, 414, 574, 599
AclWI GGATC 2 cut(s) 18, 565
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 536
AfaI GTAC 2 cut(s) 145, 317
AfiI CCNNNNNNNGG 3 cut(s) 217, 272, 360
AflII CTTAAG 1 cut(s) 569
AgsI TTSAA 3 cut(s) 150, 260, 638
AjnI CCWGG 2 cut(s) 84, 305
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 6 cut(s) 110, 115, 153, 323, 383, 445
AluI AGCT 6 cut(s) 110, 115, 153, 323, 383, 445
Alw26I GTCTC 4 cut(s) 83, 327, 371, 618
AlwI GGATC 2 cut(s) 18, 565
AlwNI CAGNNNCTG 1 cut(s) 360
AoxI GGCC 2 cut(s) 99, 351
ApeKI GCWGC 2 cut(s) 245, 320
ApoI RAATTY 1 cut(s) 280
Asp700I GAANNNNTTC 3 cut(s) 48, 633, 656
AspS9I GGNCC 1 cut(s) 351
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 3 cut(s) 200, 449, 552
AsuII TTCGAA 1 cut(s) 78
BanII GRGCYC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 257, 332
BccI CCATC 3 cut(s) 133, 529, 542
BciT130I CCWGG 2 cut(s) 86, 307
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 4 cut(s) 83, 327, 371, 618
BfaI CTAG 1 cut(s) 156
BfrI CTTAAG 1 cut(s) 569
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 3 cut(s) 246, 321, 414
BlpI GCTNAGC 2 cut(s) 111, 441
BlsI GCNGC 3 cut(s) 247, 322, 415
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 3 cut(s) 86, 307, 486
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 4 cut(s) 352, 370, 433, 483
BmrFI CCNGG 3 cut(s) 86, 307, 486
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 3 cut(s) 67, 109, 379
BmuI ACTGGG 1 cut(s) 40
Bpu1102I GCTNAGC 2 cut(s) 111, 441
Bpu14I TTCGAA 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 486
Bsa29I ATCGAT 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 84, 305, 306, 435
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 272, 360
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 2 cut(s) 86, 307
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 4 cut(s) 84, 305, 306, 435
BseGI GGATG 2 cut(s) 394, 553
BseLI CCNNNNNNNGG 3 cut(s) 217, 272, 360
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 2 cut(s) 102, 315
BseNI ACTGG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 257, 332
BshFI GGCC 2 cut(s) 101, 353
BshVI ATCGAT 1 cut(s) 47
BsiSI CCGG 2 cut(s) 393, 485
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 217, 272, 360
BsmAI GTCTC 4 cut(s) 83, 327, 371, 618
BsmBI CGTCTC 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 101, 353
Bso31I GGTCTC 1 cut(s) 327
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 2 cut(s) 23, 557
Bsp1720I GCTNAGC 2 cut(s) 111, 441
BspACI CCGC 4 cut(s) 273, 414, 574, 599
BspANI GGCC 2 cut(s) 101, 353
BspCNI CTCAG 2 cut(s) 103, 316
BspDI ATCGAT 1 cut(s) 47
BspLI GGNNCC 4 cut(s) 352, 370, 433, 483
BspPI GGATC 2 cut(s) 18, 565
BspT104I TTCGAA 1 cut(s) 78
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 327
BsrBI CCGCTC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 4 cut(s) 84, 305, 306, 435
BssMI GATC 2 cut(s) 23, 557
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 86, 307
BstAFI CTTAAG 1 cut(s) 569
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 394, 470
BstDEI CTNAG 3 cut(s) 111, 324, 441
BstF5I GGATG 2 cut(s) 394, 553
BstKTI GATC 2 cut(s) 26, 560
BstMAI GTCTC 4 cut(s) 83, 327, 371, 618
BstMBI GATC 2 cut(s) 23, 557
BstMWI GCNNNNNNNGC 4 cut(s) 107, 389, 398, 605
BstNI CCWGG 2 cut(s) 86, 307
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 3 cut(s) 84, 305, 484
BstV1I GCAGC 2 cut(s) 257, 332
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 2 cut(s) 101, 353
BsuTUI ATCGAT 1 cut(s) 47
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 394, 553
BtsIMutI CAGTG 1 cut(s) 512
Cac8I GCNNGC 2 cut(s) 394, 470
CaiI CAGNNNCTG 1 cut(s) 360
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 351
ClaI ATCGAT 1 cut(s) 47
Csp6I GTAC 2 cut(s) 144, 316
CviAII CATG 3 cut(s) 16, 212, 473
CviQI GTAC 2 cut(s) 144, 316
DdeI CTNAG 3 cut(s) 111, 324, 441
DpnI GATC 2 cut(s) 25, 559
DpnII GATC 2 cut(s) 23, 557
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 99
EciI GGCGGA 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 84, 305
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 3 cut(s) 19, 215, 476
FaiI YATR 4 cut(s) 17, 213, 405, 474
FaqI GGGAC 1 cut(s) 248
FatI CATG 3 cut(s) 15, 211, 472
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 246, 321, 414
FokI GGATG 2 cut(s) 401, 560
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 3 cut(s) 246, 321, 414
FspBI CTAG 1 cut(s) 156
GluI GCNGC 3 cut(s) 246, 321, 414
HaeIII GGCC 2 cut(s) 101, 353
HapII CCGG 2 cut(s) 393, 485
Hin1II CATG 3 cut(s) 19, 215, 476
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 4 cut(s) 44, 49, 357, 476
HpaII CCGG 2 cut(s) 393, 485
HphI GGTGA 3 cut(s) 200, 449, 552
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 368, 481, 547
Hpy188III TCNNGA 1 cut(s) 21
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 547
HpyAV CCTTC 1 cut(s) 614
HpyCH4V TGCA 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 389, 398, 605
HpyF3I CTNAG 3 cut(s) 111, 324, 441
Hsp92II CATG 3 cut(s) 19, 215, 476
KroI GCCGGC 1 cut(s) 392
KroNI GCCGGC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 23, 557
LmnI GCTCC 2 cut(s) 437, 481
LpnPI CCDG 9 cut(s) 16, 71, 98, 292, 319, 346, 367, 406, 498
Lsp1109I GCAGC 2 cut(s) 257, 332
LweI GCATC 3 cut(s) 67, 109, 379
MaeI CTAG 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 188
MalI GATC 2 cut(s) 25, 559
MbiI CCGCTC 1 cut(s) 273
MboI GATC 2 cut(s) 23, 557
MboII GAAGA 3 cut(s) 355, 519, 629
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 3 cut(s) 133, 280, 448
MmeI TCCRAC 3 cut(s) 51, 525, 594
MnlI CCTC 6 cut(s) 172, 232, 288, 465, 470, 548
MroNI GCCGGC 1 cut(s) 392
MroXI GAANNNNTTC 3 cut(s) 48, 633, 656
MseI TTAA 4 cut(s) 132, 420, 447, 570
MspA1I CMGCKG 2 cut(s) 323, 383
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 2 cut(s) 393, 485
MspR9I CCNGG 3 cut(s) 86, 307, 486
MvaI CCWGG 2 cut(s) 86, 307
MwoI GCNNNNNNNGC 4 cut(s) 107, 389, 398, 605
NaeI GCCGGC 1 cut(s) 394
NciI CCSGG 1 cut(s) 486
NdeII GATC 2 cut(s) 23, 557
NgoMIV GCCGGC 1 cut(s) 392
NlaIII CATG 3 cut(s) 19, 215, 476
NlaIV GGNNCC 4 cut(s) 352, 370, 433, 483
NmeAIII GCCGAG 1 cut(s) 575
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 19
NspV TTCGAA 1 cut(s) 78
PasI CCCWGGG 1 cut(s) 306
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 372
PdiI GCCGGC 1 cut(s) 394
PdmI GAANNNNTTC 3 cut(s) 48, 633, 656
PfeI GAWTC 4 cut(s) 44, 49, 357, 476
PflMI CCANNNNNTGG 2 cut(s) 217, 360
PkrI GCNGC 3 cut(s) 247, 322, 415
Psp6I CCWGG 2 cut(s) 84, 305
PspGI CCWGG 2 cut(s) 84, 305
PspN4I GGNNCC 4 cut(s) 352, 370, 433, 483
PspPI GGNCC 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 360
PsuI RGATCY 1 cut(s) 23
PvuII CAGCTG 2 cut(s) 323, 383
RsaI GTAC 2 cut(s) 145, 317
RsaNI GTAC 2 cut(s) 144, 316
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 132, 420, 447, 570
SatI GCNGC 3 cut(s) 246, 321, 414
Sau3AI GATC 2 cut(s) 23, 557
Sau96I GGNCC 1 cut(s) 351
ScaI AGTACT 1 cut(s) 145
ScrFI CCNGG 3 cut(s) 86, 307, 486
SduI GDGCHC 1 cut(s) 37
SetI ASST 9 cut(s) 112, 117, 155, 243, 325, 374, 385, 447, 462
SfaNI GCATC 3 cut(s) 67, 109, 379
SfuI TTCGAA 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 3 cut(s) 133, 280, 448
SsiI CCGC 4 cut(s) 273, 414, 574, 599
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 84, 305, 484
StyI CCWWGG 1 cut(s) 435
TaqI TCGA 5 cut(s) 5, 47, 78, 177, 237
TaqII GACCGA 1 cut(s) 423
TasI AATT 3 cut(s) 133, 280, 448
TatI WGTACW 1 cut(s) 143
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 4 cut(s) 44, 49, 357, 476
Tru1I TTAA 4 cut(s) 132, 420, 447, 570
Tru9I TTAA 4 cut(s) 132, 420, 447, 570
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 2 cut(s) 245, 320
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 1 cut(s) 519
Van91I CCANNNNNTGG 2 cut(s) 217, 360
Vha464I CTTAAG 1 cut(s) 569
XapI RAATTY 1 cut(s) 280
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 3 cut(s) 48, 633, 656
XspI CTAG 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.