RchiOBHm_Chr1g0347641

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004245
Physical Location & Seq
Forward (+)
40376770 .. 40377628
859 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57374

Sequence Viewer

Length: 720 bp
ATGTCCATAGAAACCGAAAGCTTCTCGAACTCAGACTGGGCTCTCTTGGAGTCGATCCGTCAGCATCTTCTTGACGACGATTTCGATACCCAGTTGGGTTTTTTCGCAGATTCTCCTGCTCAACGTACTTCCAGCTCTAACAGCACTGAGATATCGAACAGTGATGCGGAGATAGTGGGGCGGAACCAAGCATTCACTAGCGAATTTGAGGAGGTGGCGGCGGAGCGTGAACACCACGCGCCATCGGCACCGAGGCATTTTAGGGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAGAAGAACGGGATGAGAGTGTGGCTCGGAACCTACGAGACGGCGGAGCATGCCGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCGCGCATGATATGGAGGTGGGCCGTGTGTTTCCCAAGCGTGCCTCGCCGGAGCCGGCATCTCCGTCCTCCACGACGTCGTCTGAAGATGGCTCGCCGAAGCCAAAGAGGAGGAGGGGTGGAGGGGACTCGGCTGCGGCGAAAGCCAAGTTGGATGAAGCTGCTCGAGAGTTTCAACTGTTCCAAGTGGACTCATTCACATTTGGCAACGAATTATTGGTTGAGGATATGGTGCTCTTGTCACCATCTGAATGCTCAGACATTCTTTCTTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

239

Amino Acids

26.51

Weight (kDa)

5.56

Isoelectric Point (pI)

58.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 86 - 137 2.6e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 247
AccII CGCG 3 cut(s) 239, 296, 446
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 278
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 127, 293
AgsI TTSAA 2 cut(s) 397, 617
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 5 cut(s) 21, 135, 416, 421, 602
AluI AGCT 5 cut(s) 21, 135, 416, 421, 602
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 606
AoxI GGCC 2 cut(s) 278, 463
ApeKI GCWGC 2 cut(s) 575, 602
ApoI RAATTY 1 cut(s) 203
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 3 cut(s) 241, 446, 448
AspS9I GGNCC 2 cut(s) 310, 463
AsuHPI GGTGA 1 cut(s) 675
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 2 cut(s) 562, 589
BccI CCATC 3 cut(s) 250, 524, 694
BceAI ACGGC 3 cut(s) 265, 372, 450
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 1 cut(s) 198
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 7 cut(s) 219, 278, 297, 390, 576, 579, 603
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 7 cut(s) 220, 279, 298, 391, 577, 580, 604
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 2 cut(s) 310, 463
BmiI GGNNCC 7 cut(s) 185, 249, 311, 312, 346, 409, 495
BmrI ACTGGG 2 cut(s) 46, 85
BmsI GCATC 3 cut(s) 73, 154, 509
BmuI ACTGGG 2 cut(s) 46, 85
BplI GAGNNNNNCTC 2 cut(s) 324, 356
Bpu1102I GCTNAGC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 251, 281
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bse118I RCCGGY 1 cut(s) 496
Bse1I ACTGG 2 cut(s) 41, 91
BseDI CCNNGG 2 cut(s) 251, 281
BseGI GGATG 2 cut(s) 333, 601
BseMII CTCAG 3 cut(s) 45, 138, 711
BseNI ACTGG 2 cut(s) 41, 91
BsePI GCGCGC 1 cut(s) 444
BseRI GAGGAG 4 cut(s) 224, 286, 565, 568
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 562, 589
Bsh1236I CGCG 3 cut(s) 239, 296, 446
Bsh1285I CGRYCG 2 cut(s) 281, 385
BshFI GGCC 2 cut(s) 280, 465
BshNI GGYRCC 1 cut(s) 247
BsiEI CGRYCG 2 cut(s) 281, 385
BsiHKAI GWGCWC 1 cut(s) 678
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 3 cut(s) 369, 491, 497
BslFI GGGAC 2 cut(s) 323, 581
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 2 cut(s) 323, 581
BsmI GAATGC 2 cut(s) 191, 698
BsnI GGCC 2 cut(s) 280, 465
BsoBI CYCGRG 1 cut(s) 606
Bsp1286I GDGCHC 2 cut(s) 43, 678
Bsp143I GATC 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 417
BspANI GGCC 2 cut(s) 280, 465
BspCNI CTCAG 3 cut(s) 44, 139, 710
BspFNI CGCG 3 cut(s) 239, 296, 446
BspLI GGNNCC 7 cut(s) 185, 249, 311, 312, 346, 409, 495
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 496
BsrI ACTGG 2 cut(s) 41, 91
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 251, 281
BssHII GCGCGC 1 cut(s) 444
BssMI GATC 1 cut(s) 54
BssNI GRCGYC 1 cut(s) 518
Bst4CI ACNGT 2 cut(s) 161, 621
BstACI GRCGYC 1 cut(s) 518
BstC8I GCNNGC 5 cut(s) 366, 446, 483, 498, 536
BstDEI CTNAG 4 cut(s) 31, 147, 417, 697
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 333, 601
BstFNI CGCG 3 cut(s) 239, 296, 446
BstHHI GCGC 3 cut(s) 241, 446, 448
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 1 cut(s) 54
BstMCI CGRYCG 2 cut(s) 281, 385
BstMWI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 584
BstNSI RCATGY 1 cut(s) 368
BstUI CGCG 3 cut(s) 239, 296, 446
BstV1I GCAGC 2 cut(s) 562, 589
BstZI CGGCCG 1 cut(s) 278
BsuRI GGCC 2 cut(s) 280, 465
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 2 cut(s) 333, 601
BtsIMutI CAGTG 2 cut(s) 144, 166
Cac8I GCNNGC 5 cut(s) 366, 446, 483, 498, 536
CfoI GCGC 3 cut(s) 241, 446, 448
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 2 cut(s) 310, 463
Csp6I GTAC 2 cut(s) 126, 292
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 2 cut(s) 365, 449
CviQI GTAC 2 cut(s) 126, 292
DdeI CTNAG 4 cut(s) 31, 147, 417, 697
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
EaeI YGGCCR 1 cut(s) 278
EagI CGGCCG 1 cut(s) 278
EciI GGCGGA 4 cut(s) 196, 236, 314, 374
EclXI CGGCCG 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 310
EcoRV GATATC 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 2 cut(s) 368, 452
FaiI YATR 5 cut(s) 8, 366, 450, 455, 671
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 410, 442
FaqI GGGAC 2 cut(s) 323, 581
FatI CATG 2 cut(s) 364, 448
Fnu4HI GCNGC 7 cut(s) 219, 278, 297, 390, 576, 579, 603
FokI GGATG 2 cut(s) 340, 608
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 7 cut(s) 219, 278, 297, 390, 576, 579, 603
FspBI CTAG 1 cut(s) 198
GlaI GCGC 3 cut(s) 240, 445, 447
GluI GCNGC 7 cut(s) 219, 278, 297, 390, 576, 579, 603
HaeIII GGCC 2 cut(s) 280, 465
HapII CCGG 3 cut(s) 369, 491, 497
HhaI GCGC 3 cut(s) 241, 446, 448
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 2 cut(s) 368, 452
Hin6I GCGC 3 cut(s) 239, 444, 446
HinP1I GCGC 3 cut(s) 239, 444, 446
HindIII AAGCTT 2 cut(s) 19, 419
HinfI GANTC 4 cut(s) 50, 110, 569, 632
HpaII CCGG 3 cut(s) 369, 491, 497
HphI GGTGA 1 cut(s) 675
Hpy166II GTNNAC 2 cut(s) 230, 631
Hpy188I TCNGA 5 cut(s) 34, 344, 526, 691, 700
Hpy188III TCNNGA 3 cut(s) 25, 71, 608
Hpy8I GTNNAC 2 cut(s) 230, 631
Hpy99I CGWCG 3 cut(s) 80, 520, 523
HpyCH4III ACNGT 2 cut(s) 161, 621
HpyCH4IV ACGT 2 cut(s) 124, 518
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 584
HpyF3I CTNAG 4 cut(s) 31, 147, 417, 697
HpySE526I ACGT 2 cut(s) 124, 518
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 2 cut(s) 368, 452
HspAI GCGC 3 cut(s) 239, 444, 446
KflI GGGWCCC 1 cut(s) 310
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 4 cut(s) 223, 361, 413, 493
LpnPI CCDG 7 cut(s) 22, 104, 129, 145, 382, 504, 510
Lsp1109I GCAGC 2 cut(s) 562, 589
LweI GCATC 3 cut(s) 73, 154, 509
MaeI CTAG 1 cut(s) 198
MaeII ACGT 2 cut(s) 124, 518
MaeIII GTNAC 1 cut(s) 681
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 4 cut(s) 59, 328, 331, 539
MhlI GDGCHC 2 cut(s) 43, 678
MluCI AATT 2 cut(s) 203, 653
MlyI GAGTC 3 cut(s) 59, 563, 626
MmeI TCCRAC 1 cut(s) 573
MroNI GCCGGC 1 cut(s) 496
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 691
MspI CCGG 3 cut(s) 369, 491, 497
Mva1269I GAATGC 2 cut(s) 191, 698
MvnI CGCG 3 cut(s) 239, 296, 446
MwoI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 584
NaeI GCCGGC 1 cut(s) 498
NdeII GATC 1 cut(s) 54
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 2 cut(s) 368, 452
NlaIV GGNNCC 7 cut(s) 185, 249, 311, 312, 346, 409, 495
NmeAIII GCCGAG 1 cut(s) 551
NmuCI GTSAC 1 cut(s) 681
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 606
PauI GCGCGC 1 cut(s) 444
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 348, 578
PctI GAATGC 2 cut(s) 191, 698
PdiI GCCGGC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 520
PkrI GCNGC 7 cut(s) 220, 279, 298, 391, 577, 580, 604
PleI GAGTC 3 cut(s) 58, 563, 626
PpsI GAGTC 3 cut(s) 58, 563, 626
PpuMI RGGWCCY 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 310
PspN4I GGNNCC 7 cut(s) 185, 249, 311, 312, 346, 409, 495
PspPI GGNCC 2 cut(s) 310, 463
PspPPI RGGWCCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 520
PteI GCGCGC 1 cut(s) 444
RsaI GTAC 2 cut(s) 127, 293
RsaNI GTAC 2 cut(s) 126, 292
RseI CAYNNNNRTG 1 cut(s) 691
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 7 cut(s) 219, 278, 297, 390, 576, 579, 603
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 310, 463
SchI GAGTC 3 cut(s) 59, 563, 626
SduI GDGCHC 2 cut(s) 43, 678
SfaNI GCATC 3 cut(s) 73, 154, 509
Sfr274I CTCGAG 1 cut(s) 606
SinI GGWCC 1 cut(s) 310
SlaI CTCGAG 1 cut(s) 606
SmiMI CAYNNNNRTG 1 cut(s) 691
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
SphI GCATGC 1 cut(s) 368
Sse9I AATT 2 cut(s) 203, 653
SspMI CTAG 1 cut(s) 198
TaaI ACNGT 2 cut(s) 161, 621
TaiI ACGT 2 cut(s) 127, 521
TaqI TCGA 5 cut(s) 26, 53, 84, 155, 607
TaqII GACCGA 1 cut(s) 399
TasI AATT 2 cut(s) 203, 653
TauI GCSGC 5 cut(s) 221, 280, 299, 392, 581
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 2 cut(s) 151, 166
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 2 cut(s) 575, 602
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 1 cut(s) 612
TspGWI ACGGA 2 cut(s) 47, 495
TspRI CASTG 2 cut(s) 151, 166
Tth111I GACNNNGTC 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 203
XceI RCATGY 1 cut(s) 368
XhoI CTCGAG 1 cut(s) 606
XspI CTAG 1 cut(s) 198
ZraI GACGTC 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.