RLG00000028721

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004245
Physical Location & Seq
Reverse (-)
26975292 .. 26976042
751 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028721

Sequence Viewer

Length: 624 bp
ATGAAGTCTCAGAGAAGCAACTGTGCCCATTTAAAATTTTTAATGACATGGTTTGCAAGCAATGCCGAAACTGAAAGCTTCTCAAACTCGAACTGGGCTCTCTTGGAATCCATCCGCCAACATCTTCTAGACGACGATTTTGAATTTGATTCCCAGTCGGCATTTTTTGCAAACTCTCCTGCTCAAAGTACTTCAAGCTCTAGCAGAATTGAAATATCGAGCAACGACTTGGAGGTCGTGGAGGAGCCGAACCAAGTGTTCAAGTGTAAACTGGACGTGGCGGAGCGTGAACACAACACGCCGTCGAGGCAGAGGCATAATTTTAGGGGCGTGAGGAGGCGGCCATGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAAAAGAACGGGACCAGAGTGTGGCTCGGAACCTATGAGAGGGCGGAGGACGCAGGCTTGGCTTACGACCGAGCTGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCACGAATGATATGGAAGTGGGCCGTGTCCTTCCCAAGCGTGCCTCGCCGGAGTCATCATCTCCGTCGTCAGAAGATGGCTCGCCGAAGCCTAAGAGGAGGAGGAGTGGAGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

208

Amino Acids

23.5

Weight (kDa)

9.44

Isoelectric Point (pI)

66.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 108 - 158 3.6e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 399
AccII CGCG 1 cut(s) 359
AciI CCGC 6 cut(s) 115, 281, 340, 359, 362, 422
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 2 cut(s) 35, 143
AfaI GTAC 2 cut(s) 190, 356
AfiI CCNNNNNNNGG 2 cut(s) 399, 417
AgsI TTSAA 5 cut(s) 143, 195, 212, 262, 460
AjiI CACGTC 1 cut(s) 277
AluBI AGCT 5 cut(s) 78, 198, 452, 479, 484
AluI AGCT 5 cut(s) 78, 198, 452, 479, 484
Alw26I GTCTC 1 cut(s) 12
AoxI GGCC 2 cut(s) 341, 526
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 2 cut(s) 35, 143
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AspS9I GGNCC 3 cut(s) 373, 390, 526
AvaII GGWCC 2 cut(s) 373, 390
BaeGI GKGCMC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 439
BccI CCATC 2 cut(s) 119, 575
BceAI ACGGC 2 cut(s) 286, 513
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 3 cut(s) 128, 201, 622
BglI GCCNNNNNGGC 1 cut(s) 307
BisI GCNGC 3 cut(s) 341, 360, 453
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 3 cut(s) 342, 361, 454
BmcAI AGTACT 1 cut(s) 190
Bme18I GGWCC 2 cut(s) 373, 390
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 3 cut(s) 373, 390, 526
BmiI GGNNCC 6 cut(s) 246, 374, 375, 391, 409, 472
BmrI ACTGGG 2 cut(s) 103, 148
BmuI ACTGGG 2 cut(s) 103, 148
BplI GAGNNNNNCTC 2 cut(s) 387, 419
Bpu1102I GCTNAGC 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 344
BsaXI ACNNNNNCTCC 1 cut(s) 595
Bsc4I CCNNNNNNNGG 2 cut(s) 399, 417
Bse1I ACTGG 3 cut(s) 98, 154, 276
Bse3DI GCAATG 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 399, 417
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 3 cut(s) 98, 154, 276
BseRI GAGGAG 5 cut(s) 257, 349, 616, 619, 622
BseSI GKGCMC 1 cut(s) 28
BseXI GCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 448
BshFI GGCC 2 cut(s) 343, 528
BsiEI CGRYCG 1 cut(s) 448
BsiSI CCGG 1 cut(s) 554
BslFI GGGAC 2 cut(s) 386, 403
BslI CCNNNNNNNGG 2 cut(s) 399, 417
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 386, 403
BsnI GGCC 2 cut(s) 343, 528
Bsp1286I GDGCHC 2 cut(s) 28, 100
Bsp1720I GCTNAGC 1 cut(s) 480
Bsp19I CCATGG 1 cut(s) 344
BspACI CCGC 6 cut(s) 115, 281, 340, 359, 362, 422
BspANI GGCC 2 cut(s) 343, 528
BspCNI CTCAG 1 cut(s) 22
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 6 cut(s) 246, 374, 375, 391, 409, 472
BsrDI GCAATG 1 cut(s) 67
BsrI ACTGG 3 cut(s) 98, 154, 276
BssECI CCNNGG 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 23
BstAPI GCANNNNNTGC 2 cut(s) 62, 167
BstC8I GCNNGC 4 cut(s) 58, 433, 546, 587
BstDEI CTNAG 3 cut(s) 9, 480, 597
BstDSI CCRYGG 1 cut(s) 344
BstENI CCTNNNNNAGG 1 cut(s) 415
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 359
BstMAI GTCTC 1 cut(s) 12
BstMCI CGRYCG 1 cut(s) 448
BstMWI GCNNNNNNNGC 6 cut(s) 62, 167, 307, 428, 437, 550
BstSLI GKGCMC 1 cut(s) 28
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 439
BsuRI GGCC 2 cut(s) 343, 528
BtgI CCRYGG 1 cut(s) 344
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 111
Cac8I GCNNGC 4 cut(s) 58, 433, 546, 587
Cfr13I GGNCC 3 cut(s) 373, 390, 526
CseI GACGC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 189, 355
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 2 cut(s) 48, 345
CviQI GTAC 2 cut(s) 189, 355
DdeI CTNAG 3 cut(s) 9, 480, 597
DraI TTTAAA 1 cut(s) 33
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 341
EciI GGCGGA 4 cut(s) 104, 296, 377, 437
Eco130I CCWWGG 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 373, 390
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 344
FaeI CATG 2 cut(s) 51, 348
FaiI YATR 5 cut(s) 49, 318, 346, 414, 518
FalI AAGNNNNNCTT 4 cut(s) 468, 500, 473, 505
FaqI GGGAC 2 cut(s) 386, 403
FatI CATG 2 cut(s) 47, 344
Fnu4HI GCNGC 3 cut(s) 341, 360, 453
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 3 cut(s) 341, 360, 453
FspBI CTAG 3 cut(s) 128, 201, 622
GluI GCNGC 3 cut(s) 341, 360, 453
HaeIII GGCC 2 cut(s) 343, 528
HapII CCGG 1 cut(s) 554
HgaI GACGC 1 cut(s) 437
Hin1II CATG 2 cut(s) 51, 348
HindIII AAGCTT 2 cut(s) 76, 482
HinfI GANTC 3 cut(s) 107, 149, 557
HpaII CCGG 1 cut(s) 554
Hpy166II GTNNAC 3 cut(s) 269, 290, 619
Hpy188I TCNGA 3 cut(s) 12, 407, 577
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 3 cut(s) 269, 290, 619
Hpy99I CGWCG 3 cut(s) 137, 307, 574
HpyAV CCTTC 1 cut(s) 545
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 56, 170
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 167, 307, 428, 437, 550
HpyF3I CTNAG 3 cut(s) 9, 480, 597
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 2 cut(s) 51, 348
KflI GGGWCCC 1 cut(s) 373
LmnI GCTCC 3 cut(s) 244, 283, 476
LpnPI CCDG 7 cut(s) 79, 167, 192, 257, 406, 417, 567
Lsp1109I GCAGC 1 cut(s) 439
MaeI CTAG 3 cut(s) 128, 201, 622
MaeII ACGT 1 cut(s) 276
MboII GAAGA 2 cut(s) 116, 590
MhlI GDGCHC 2 cut(s) 28, 100
MluCI AATT 4 cut(s) 35, 143, 207, 319
MlyI GAGTC 1 cut(s) 566
MseI TTAA 3 cut(s) 32, 41, 486
MspI CCGG 1 cut(s) 554
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 6 cut(s) 62, 167, 307, 428, 437, 550
NcoI CCATGG 1 cut(s) 344
NlaIII CATG 2 cut(s) 51, 348
NlaIV GGNNCC 6 cut(s) 246, 374, 375, 391, 409, 472
PfeI GAWTC 2 cut(s) 107, 149
PflMI CCANNNNNTGG 1 cut(s) 399
PkrI GCNGC 3 cut(s) 342, 361, 454
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
PspN4I GGNNCC 6 cut(s) 246, 374, 375, 391, 409, 472
PspPI GGNCC 3 cut(s) 373, 390, 526
PspPPI RGGWCCY 1 cut(s) 373
RsaI GTAC 2 cut(s) 190, 356
RsaNI GTAC 2 cut(s) 189, 355
SaqAI TTAA 3 cut(s) 32, 41, 486
SatI GCNGC 3 cut(s) 341, 360, 453
Sau96I GGNCC 3 cut(s) 373, 390, 526
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 1 cut(s) 566
SduI GDGCHC 2 cut(s) 28, 100
SetI ASST 8 cut(s) 80, 200, 237, 279, 413, 454, 481, 486
SinI GGWCC 2 cut(s) 373, 390
Sse9I AATT 4 cut(s) 35, 143, 207, 319
SsiI CCGC 6 cut(s) 115, 281, 340, 359, 362, 422
SspMI CTAG 3 cut(s) 128, 201, 622
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 1 cut(s) 23
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 89, 218, 305
TaqII GACCGA 1 cut(s) 462
TasI AATT 4 cut(s) 35, 143, 207, 319
TatI WGTACW 1 cut(s) 188
TauI GCSGC 2 cut(s) 343, 362
TfiI GAWTC 2 cut(s) 107, 149
Tru1I TTAA 3 cut(s) 32, 41, 486
Tru9I TTAA 3 cut(s) 32, 41, 486
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 558
Van91I CCANNNNNTGG 1 cut(s) 399
VpaK11BI GGWCC 2 cut(s) 373, 390
XagI CCTNNNNNAGG 1 cut(s) 415
XapI RAATTY 2 cut(s) 35, 143
XbaI TCTAGA 1 cut(s) 127
XspI CTAG 3 cut(s) 128, 201, 622
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.