MD07G1106200.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004245
Physical Location & Seq
Forward (+)
12029354 .. 12030067
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1106200.v1.1.491

Sequence Viewer

Length: 714 bp
ATGTCAACAGAAAGCTTCTCAAGTTCTAACTGGGCTTTCTTGGAGTCGATTCGTCAACATCTGCTGCACGATGATTTCGAAATACAGGAAACATTTTTGGCCAATGTAGCTCAGCTTGATGCACCTTTGCATAATTCTCCAAGTACTTCGAGCTCTAGCAGTGCTCATTTTGTTGTGGAGGGTTCGGGTAACACAATGGGAGTAATGAACCACGCTTTGGAGACATCAAATAAGTTCGAGTTGCCGGTGGGGACTGTTCAATCACCGTTCTGCGGGCGCCATTTTAGGGGTGTCAGGCGACGACCATGGGGGAAGTACGCAGCGGAGATAAGAGACCCAAAGAAGAACGGGGCGAGAATCTGGCTCGGAACCTATGAGACGGCAGAGGATGCCGGCTTGGCTTATGACCGAGCCGCTTTTAAAATGCGTGGCTCCAAGGCTAAGCTTAATTTCCCTCACCTCATTGGCACACATGATACGGAGCCGGGTCGTGTCGCAACTAAACGTTGCTCTCCGGAGCCATTATCTTCATCGGTCTCATCTTCTGATGGTAGCGGCTCACCAATGCTTAAGCGGAGAAGGATTGTAGTTAGCTCGGCGGCTAAAGCCAAATTGGAAGTTGCAGATGAACAGCTTCAACTTAACTCTTTCTCATTTGGCAGCCATCTATTGCTTAACGAGTTGTATACATTTGACGATCTTTTTGCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

238

Amino Acids

26.11

Weight (kDa)

7.14

Isoelectric Point (pI)

55.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 94 - 145 2.5e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 276
AccB7I CCANNNNNTGG 1 cut(s) 217
AccI GTMKAC 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 6 cut(s) 273, 323, 414, 555, 574, 599
AclI AACGTT 1 cut(s) 505
AcoI YGGCCR 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 277
AfaI GTAC 2 cut(s) 145, 317
AfiI CCNNNNNNNGG 3 cut(s) 217, 272, 286
AflII CTTAAG 1 cut(s) 569
AgsI TTSAA 2 cut(s) 260, 638
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 7 cut(s) 15, 110, 115, 153, 445, 594, 634
AluI AGCT 7 cut(s) 15, 110, 115, 153, 445, 594, 634
Alw21I GWGCWC 2 cut(s) 155, 166
Alw26I GTCTC 4 cut(s) 215, 327, 371, 541
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 3 cut(s) 64, 320, 660
ArsI GACNNNNNNTTYG 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 1 cut(s) 279
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 3 cut(s) 255, 449, 552
AsuII TTCGAA 1 cut(s) 78
BalI TGGCCA 1 cut(s) 101
BanI GGYRCC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 155, 166
BbvI GCAGC 3 cut(s) 51, 332, 672
BccI CCATC 2 cut(s) 542, 672
BceAI ACGGC 1 cut(s) 396
BcgI CGANNNNNNTGC 1 cut(s) 686
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 4 cut(s) 215, 327, 371, 541
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 280
BfrI CTTAAG 1 cut(s) 569
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 6 cut(s) 65, 321, 414, 556, 600, 661
BlpI GCTNAGC 2 cut(s) 111, 441
BlsI GCNGC 6 cut(s) 66, 322, 415, 557, 601, 662
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 486
BmiI GGNNCC 5 cut(s) 278, 370, 433, 483, 519
BmrFI CCNGG 1 cut(s) 486
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 2 cut(s) 109, 379
BmuI ACTGGG 1 cut(s) 40
Bpu1102I GCTNAGC 2 cut(s) 111, 441
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 4
BpuMI CCSGG 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 277
BsaI GGTCTC 2 cut(s) 327, 541
BsaJI CCNNGG 2 cut(s) 305, 435
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 272, 286
Bse118I RCCGGY 2 cut(s) 244, 392
Bse1I ACTGG 1 cut(s) 35
BseAI TCCGGA 1 cut(s) 514
BseDI CCNNGG 2 cut(s) 305, 435
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 3 cut(s) 217, 272, 286
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 1 cut(s) 35
BseXI GCAGC 3 cut(s) 51, 332, 672
BsgI GTGCAG 1 cut(s) 50
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 155, 166
BsiSI CCGG 4 cut(s) 245, 393, 485, 515
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 3 cut(s) 217, 272, 286
BsmAI GTCTC 4 cut(s) 215, 327, 371, 541
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 2 cut(s) 327, 541
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 155, 166
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 1 cut(s) 697
Bsp1720I GCTNAGC 2 cut(s) 111, 441
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 6 cut(s) 273, 323, 414, 555, 574, 599
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 124
BspEI TCCGGA 1 cut(s) 514
BspLI GGNNCC 5 cut(s) 278, 370, 433, 483, 519
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 2 cut(s) 327, 541
BsrFI RCCGGY 2 cut(s) 244, 392
BsrI ACTGG 1 cut(s) 35
BssAI RCCGGY 2 cut(s) 244, 392
BssECI CCNNGG 2 cut(s) 305, 435
BssMI GATC 1 cut(s) 697
BssNAI GTATAC 1 cut(s) 687
BssNI GRCGYC 1 cut(s) 277
BssT1I CCWWGG 2 cut(s) 305, 435
Bst1107I GTATAC 1 cut(s) 687
Bst4CI ACNGT 2 cut(s) 256, 267
BstACI GRCGYC 1 cut(s) 277
BstAFI CTTAAG 1 cut(s) 569
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 275, 394
BstDEI CTNAG 2 cut(s) 111, 441
BstDSI CCRYGG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 280
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 1 cut(s) 700
BstMAI GTCTC 4 cut(s) 215, 327, 371, 541
BstMBI GATC 1 cut(s) 697
BstMWI GCNNNNNNNGC 4 cut(s) 107, 389, 398, 605
BstSCI CCNGG 1 cut(s) 484
BstV1I GCAGC 3 cut(s) 51, 332, 672
BstZ17I GTATAC 1 cut(s) 687
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 305
BtsCI GGATG 1 cut(s) 394
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 2 cut(s) 275, 394
CfoI GCGC 1 cut(s) 279
Cfr10I RCCGGY 2 cut(s) 244, 392
Csp6I GTAC 2 cut(s) 144, 316
CviAII CATG 2 cut(s) 306, 473
CviQI GTAC 2 cut(s) 144, 316
DdeI CTNAG 2 cut(s) 111, 441
DinI GGCGCC 1 cut(s) 278
DpnI GATC 1 cut(s) 699
DpnII GATC 1 cut(s) 697
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 153
Eco130I CCWWGG 2 cut(s) 305, 435
Eco24I GRGCYC 1 cut(s) 155
Eco31I GGTCTC 2 cut(s) 327, 541
Eco53kI GAGCTC 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 153
EcoT14I CCWWGG 2 cut(s) 305, 435
EcoT38I GRGCYC 1 cut(s) 155
EgeI GGCGCC 1 cut(s) 278
EheI GGCGCC 1 cut(s) 278
ErhI CCWWGG 2 cut(s) 305, 435
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 309, 476
FaiI YATR 6 cut(s) 132, 307, 375, 405, 474, 687
FaqI GGGAC 1 cut(s) 265
FatI CATG 2 cut(s) 305, 472
FauI CCCGC 1 cut(s) 266
FblI GTMKAC 1 cut(s) 686
Fnu4HI GCNGC 6 cut(s) 65, 321, 414, 556, 600, 661
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 6 cut(s) 65, 321, 414, 556, 600, 661
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 278
GluI GCNGC 6 cut(s) 65, 321, 414, 556, 600, 661
HaeII RGCGCY 1 cut(s) 280
HaeIII GGCC 1 cut(s) 101
HapII CCGG 4 cut(s) 245, 393, 485, 515
HhaI GCGC 1 cut(s) 279
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 2 cut(s) 309, 476
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HincII GTYRAC 2 cut(s) 6, 56
HindII GTYRAC 2 cut(s) 6, 56
HindIII AAGCTT 2 cut(s) 13, 443
HinfI GANTC 3 cut(s) 44, 49, 357
HpaII CCGG 4 cut(s) 245, 393, 485, 515
HphI GGTGA 3 cut(s) 255, 449, 552
Hpy166II GTNNAC 3 cut(s) 6, 56, 687
Hpy188I TCNGA 2 cut(s) 368, 547
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 3 cut(s) 6, 56, 687
Hpy99I CGWCG 1 cut(s) 303
HpyAV CCTTC 1 cut(s) 573
HpyCH4III ACNGT 2 cut(s) 256, 267
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 4 cut(s) 67, 122, 130, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 389, 398, 605
HpyF3I CTNAG 2 cut(s) 111, 441
HpySE526I ACGT 1 cut(s) 505
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 2 cut(s) 309, 476
HspAI GCGC 1 cut(s) 277
KasI GGCGCC 1 cut(s) 276
Kpn2I TCCGGA 1 cut(s) 514
KroI GCCGGC 1 cut(s) 392
KroNI GCCGGC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 697
LmnI GCTCC 3 cut(s) 437, 481, 517
LpnPI CCDG 8 cut(s) 16, 71, 258, 280, 346, 406, 498, 528
Lsp1109I GCAGC 3 cut(s) 51, 332, 672
LweI GCATC 2 cut(s) 109, 379
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 1 cut(s) 188
MalI GATC 1 cut(s) 699
MboI GATC 1 cut(s) 697
MboII GAAGA 3 cut(s) 355, 519, 534
MhlI GDGCHC 2 cut(s) 155, 166
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 3 cut(s) 133, 448, 611
MluNI TGGCCA 1 cut(s) 101
Mly113I GGCGCC 1 cut(s) 277
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 4 cut(s) 172, 379, 465, 470
Mox20I TGGCCA 1 cut(s) 101
MroI TCCGGA 1 cut(s) 514
MroNI GCCGGC 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 633
MscI TGGCCA 1 cut(s) 101
MseI TTAA 6 cut(s) 420, 447, 570, 642, 675, 712
Msp20I TGGCCA 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 4 cut(s) 245, 393, 485, 515
MspR9I CCNGG 1 cut(s) 486
MwoI GCNNNNNNNGC 4 cut(s) 107, 389, 398, 605
NaeI GCCGGC 1 cut(s) 394
NarI GGCGCC 1 cut(s) 277
NciI CCSGG 1 cut(s) 486
NcoI CCATGG 1 cut(s) 305
NdeII GATC 1 cut(s) 697
NgoMIV GCCGGC 1 cut(s) 392
NlaIII CATG 2 cut(s) 309, 476
NlaIV GGNNCC 5 cut(s) 278, 370, 433, 483, 519
NmeAIII GCCGAG 1 cut(s) 575
NspV TTCGAA 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdiI GCCGGC 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 49, 357
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 6 cut(s) 66, 322, 415, 557, 601, 662
PleI GAGTC 1 cut(s) 52
PluTI GGCGCC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 52
Psp124BI GAGCTC 1 cut(s) 155
Psp1406I AACGTT 1 cut(s) 505
PspN4I GGNNCC 5 cut(s) 278, 370, 433, 483, 519
RsaI GTAC 2 cut(s) 145, 317
RsaNI GTAC 2 cut(s) 144, 316
SacI GAGCTC 1 cut(s) 155
SaqAI TTAA 6 cut(s) 420, 447, 570, 642, 675, 712
SatI GCNGC 6 cut(s) 65, 321, 414, 556, 600, 661
Sau3AI GATC 1 cut(s) 697
ScaI AGTACT 1 cut(s) 145
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 2 cut(s) 155, 166
SfaNI GCATC 2 cut(s) 109, 379
SfoI GGCGCC 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 19, 569
SmoI CTYRAG 2 cut(s) 19, 569
Sse9I AATT 3 cut(s) 133, 448, 611
SsiI CCGC 6 cut(s) 273, 323, 414, 555, 574, 599
SspDI GGCGCC 1 cut(s) 276
SspMI CTAG 1 cut(s) 156
SstI GAGCTC 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 2 cut(s) 305, 435
TaaI ACNGT 2 cut(s) 256, 267
TaiI ACGT 1 cut(s) 508
TaqI TCGA 4 cut(s) 47, 78, 149, 237
TaqII GACCGA 2 cut(s) 423, 523
TasI AATT 3 cut(s) 133, 448, 611
TatI WGTACW 1 cut(s) 143
TauI GCSGC 3 cut(s) 416, 558, 602
TfiI GAWTC 2 cut(s) 49, 357
Tru1I TTAA 6 cut(s) 420, 447, 570, 642, 675, 712
Tru9I TTAA 6 cut(s) 420, 447, 570, 642, 675, 712
TscAI CASTG 1 cut(s) 166
TseI GCWGC 3 cut(s) 64, 320, 660
TspDTI ATGAA 3 cut(s) 221, 519, 642
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 166
Van91I CCANNNNNTGG 1 cut(s) 217
Vha464I CTTAAG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.