RchiOBHm_Chr1g0347661

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004245
Physical Location & Seq
Forward (+)
40381921 .. 40382727
807 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57376

Sequence Viewer

Length: 654 bp
ATGTCCACAGAAACAAGCTTCTCAAACTCAGACTGGGCTCTCTTGGAATCGATCCGCCAGCATCTTCTCGAAGACGATTTCGATACCCAGTCGGCATTTTCCACTGACTCTCCAGCTCAAAATACTTCCAGCTCTAACTGCACTGAGATATCGAACAGTGATGCGGAGATAGTGGAGCAGAACCAAGCATTCACTAGTGAATTTGAGGCGGAGGTGGCGGCGCGTGAACACCACACACCATCGGGTCAGAGGCATTTTAGAGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAAAAGAACGGGACGAGAGTGTGGCTCGGGACCTACGAGAAGGCGGAGGACGCAGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAATTTTCCTCACTTGATTGGCATGCATGATGTGGAGGTGGGCCGTGTGCTTCCCAAGCGTGCCTCGCCGGAGCCGTCATCTCCGTCCTCCACGACGTCGACTGAAGATGGCTCGCCGAAGCCAAAGGGGTGGAGTGGGCTTGGCTGCGGCGGAAGCCCAGTTGGAGGAAGCTGCTCAAGAGTTTCATGTGTTCCAAGTGGACTCATTCACATTTTGCAACGAACTATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

217

Amino Acids

23.6

Weight (kDa)

6.39

Isoelectric Point (pI)

65.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 85 - 136 8.6e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 518
AccI GTMKAC 1 cut(s) 518
AccII CGCG 2 cut(s) 223, 293
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 584
AgsI TTSAA 1 cut(s) 394
AhlI ACTAGT 1 cut(s) 194
AluBI AGCT 6 cut(s) 18, 116, 132, 413, 418, 591
AluI AGCT 6 cut(s) 18, 116, 132, 413, 418, 591
AlwI GGATC 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 2 cut(s) 275, 460
ApeKI GCWGC 2 cut(s) 564, 591
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 307, 342, 460
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 307, 342
BanII GRGCYC 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 551, 578
BccI CCATC 2 cut(s) 247, 521
BceAI ACGGC 3 cut(s) 262, 447, 478
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 1 cut(s) 195
BisI GCNGC 7 cut(s) 219, 275, 294, 387, 565, 568, 592
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 7 cut(s) 220, 276, 295, 388, 566, 569, 593
Bme18I GGWCC 2 cut(s) 307, 342
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 3 cut(s) 307, 342, 460
BmiI GGNNCC 5 cut(s) 308, 309, 343, 406, 492
BmrI ACTGGG 3 cut(s) 43, 82, 572
BmsI GCATC 2 cut(s) 70, 151
BmuI ACTGGG 3 cut(s) 43, 82, 572
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 321, 353
BpmI CTGGAG 1 cut(s) 96
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 580
Bsa29I ATCGAT 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 3 cut(s) 38, 88, 578
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 278
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 2 cut(s) 42, 135
BseNI ACTGG 3 cut(s) 38, 88, 578
BseRI GAGGAG 2 cut(s) 276, 283
BseX3I CGGCCG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 551, 578
BsgI GTGCAG 1 cut(s) 124
Bsh1236I CGCG 2 cut(s) 223, 293
Bsh1285I CGRYCG 2 cut(s) 278, 382
BshFI GGCC 2 cut(s) 277, 462
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 2 cut(s) 278, 382
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 3 cut(s) 320, 337, 355
BslI CCNNNNNNNGG 1 cut(s) 584
BsmFI GGGAC 3 cut(s) 320, 337, 355
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 2 cut(s) 277, 462
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 1 cut(s) 51
Bsp1720I GCTNAGC 1 cut(s) 414
BspANI GGCC 2 cut(s) 277, 462
BspCNI CTCAG 2 cut(s) 41, 136
BspDI ATCGAT 1 cut(s) 50
BspFNI CGCG 2 cut(s) 223, 293
BspLI GGNNCC 5 cut(s) 308, 309, 343, 406, 492
BspPI GGATC 1 cut(s) 46
BsrI ACTGG 3 cut(s) 38, 88, 578
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 515
BstC8I GCNNGC 4 cut(s) 59, 443, 480, 533
BstDEI CTNAG 3 cut(s) 28, 144, 414
BstDSI CCRYGG 1 cut(s) 278
BstFNI CGCG 2 cut(s) 223, 293
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 2 cut(s) 278, 382
BstMWI GCNNNNNNNGC 7 cut(s) 138, 215, 362, 371, 475, 484, 573
BstNSI RCATGY 1 cut(s) 445
BstUI CGCG 2 cut(s) 223, 293
BstV1I GCAGC 2 cut(s) 551, 578
BstV2I GAAGAC 1 cut(s) 78
BstXI CCANNNNNNTGG 1 cut(s) 549
BstZI CGGCCG 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 2 cut(s) 277, 462
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 278
BtsIMutI CAGTG 3 cut(s) 102, 141, 163
Cac8I GCNNGC 4 cut(s) 59, 443, 480, 533
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 3 cut(s) 307, 342, 460
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 1 cut(s) 371
Csp6I GTAC 1 cut(s) 289
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 3 cut(s) 442, 446, 606
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 3 cut(s) 28, 144, 414
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 275
EagI CGGCCG 1 cut(s) 275
EciI GGCGGA 5 cut(s) 44, 224, 311, 371, 585
EclXI CGGCCG 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 40
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 307, 342
Eco52I CGGCCG 1 cut(s) 275
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 2 cut(s) 307, 342
EcoRV GATATC 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 40
FaeI CATG 3 cut(s) 445, 449, 609
FaiI YATR 3 cut(s) 443, 447, 607
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 3 cut(s) 320, 337, 355
FatI CATG 3 cut(s) 441, 445, 605
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 7 cut(s) 219, 275, 294, 387, 565, 568, 592
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 7 cut(s) 219, 275, 294, 387, 565, 568, 592
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 222
GluI GCNGC 7 cut(s) 219, 275, 294, 387, 565, 568, 592
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 277, 462
HapII CCGG 1 cut(s) 488
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 3 cut(s) 445, 449, 609
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 2 cut(s) 16, 416
HinfI GANTC 3 cut(s) 47, 107, 621
HpaII CCGG 1 cut(s) 488
Hpy166II GTNNAC 4 cut(s) 6, 227, 519, 620
Hpy188I TCNGA 2 cut(s) 31, 249
Hpy188III TCNNGA 3 cut(s) 68, 340, 597
Hpy8I GTNNAC 4 cut(s) 6, 227, 519, 620
Hpy99I CGWCG 2 cut(s) 517, 520
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 3 cut(s) 141, 445, 637
HpyF10VI GCNNNNNNNGC 7 cut(s) 138, 215, 362, 371, 475, 484, 573
HpyF3I CTNAG 3 cut(s) 28, 144, 414
HpySE526I ACGT 1 cut(s) 515
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 3 cut(s) 445, 449, 609
HspAI GCGC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 307
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 3 cut(s) 175, 410, 490
LpnPI CCDG 8 cut(s) 19, 71, 101, 126, 142, 351, 501, 591
Lsp1109I GCAGC 2 cut(s) 551, 578
LweI GCATC 2 cut(s) 70, 151
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 515
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 3 cut(s) 56, 83, 536
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 2 cut(s) 200, 421
MlyI GAGTC 2 cut(s) 101, 615
MmeI TCCRAC 1 cut(s) 562
Mph1103I ATGCAT 1 cut(s) 447
MseI TTAA 1 cut(s) 420
MspI CCGG 1 cut(s) 488
Mva1269I GAATGC 1 cut(s) 188
MvnI CGCG 2 cut(s) 223, 293
MwoI GCNNNNNNNGC 7 cut(s) 138, 215, 362, 371, 475, 484, 573
NdeII GATC 1 cut(s) 51
NlaIII CATG 3 cut(s) 445, 449, 609
NlaIV GGNNCC 5 cut(s) 308, 309, 343, 406, 492
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 1 cut(s) 445
PaeI GCATGC 1 cut(s) 445
PcsI WCGNNNNNNNCGW 2 cut(s) 345, 491
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 47
PkrI GCNGC 7 cut(s) 220, 276, 295, 388, 566, 569, 593
PleI GAGTC 2 cut(s) 101, 615
PpsI GAGTC 2 cut(s) 101, 615
PpuMI RGGWCCY 2 cut(s) 307, 342
Psp5II RGGWCCY 2 cut(s) 307, 342
PspN4I GGNNCC 5 cut(s) 308, 309, 343, 406, 492
PspPI GGNCC 3 cut(s) 307, 342, 460
PspPPI RGGWCCY 2 cut(s) 307, 342
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SalI GTCGAC 1 cut(s) 517
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 7 cut(s) 219, 275, 294, 387, 565, 568, 592
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 3 cut(s) 307, 342, 460
SchI GAGTC 2 cut(s) 101, 615
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 2 cut(s) 70, 151
SinI GGWCC 2 cut(s) 307, 342
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
SpeI ACTAGT 1 cut(s) 194
SphI GCATGC 1 cut(s) 445
Sse9I AATT 2 cut(s) 200, 421
SspMI CTAG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 1 cut(s) 518
TaqI TCGA 5 cut(s) 50, 69, 81, 152, 518
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 200, 421
TauI GCSGC 5 cut(s) 221, 277, 296, 389, 570
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 3 cut(s) 109, 148, 163
TseI GCWGC 2 cut(s) 564, 591
TspDTI ATGAA 1 cut(s) 594
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 3 cut(s) 109, 148, 163
VpaK11BI GGWCC 2 cut(s) 307, 342
XapI RAATTY 1 cut(s) 200
XceI RCATGY 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 518
XspI CTAG 1 cut(s) 195
ZraI GACGTC 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.