RLG00000028724

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004245
Physical Location & Seq
Reverse (-)
27011843 .. 27014021
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028724

Sequence Viewer

Length: 846 bp
ATGTCCATAGAAACCGAAAGCGTCTCGAACTCAGACTGGGCTCTCTTGGAGTCGATCCGTCAGCATCTTCTTGACGACGATTCCGATACCCAGTTGGGCTTTTTCGCAGATTCTCCTGCTCAGAGTACTTCCAGCTCTAACAGCACTGAGGTATCGAACAGTGATGCGGAGATAGTGAAGCAGAACCAAGCATTCACCGGTGAATTTGAGGAGGAGGTGGCCGAGCGTGAACACCACGCGCCATCGGCACCGAGGCATTTTAGAGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAGAAGAACGGGATGAGAGTGTGGCTCGGAACCTACGAGACGGCGGAGGATGCCGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCACGCATGATATGGAGGTGGGCCGTGTGTTTCCCAAGCGTGCCTCGCGGGAGCCGTCATCTCCGTCCTCCACGACGTCGTCTGTAGATGGCTCGCCGAAGCCAAAGAGGAGGAGGGGTGGAGTACTGGACTCGGCTGCACCGAAAGCCAAGTTGGAGGAAGCTGCTCAAGAGTGTCATGTGTTCCAAGTGGACTCAGTCACATTTGACAACGAACTATTGGTTGAGGATATGATATCGAACACCAGCTCATCGATTCGTACCGCGACAAGTGGTACTCCTCTCGACTCGGTACTATGCAGGAAATTGAAAAGCTTGACCATGGTGGATTCTGATTCTTCTCTGATGCAAATGAAGATTTTGCTCAGGAAAATTGTTATAGCCCAGAAGTTATTAGCACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

282

Amino Acids

31.03

Weight (kDa)

6.2

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 86 - 137 1.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 247
AccII CGCG 4 cut(s) 239, 296, 490, 707
AciI CCGC 8 cut(s) 167, 277, 296, 299, 359, 390, 490, 705
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 2 cut(s) 219, 278
AcsI RAATTY 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 6 cut(s) 127, 293, 567, 703, 718, 735
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 2 cut(s) 397, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 6 cut(s) 135, 416, 421, 605, 690, 756
AluI AGCT 6 cut(s) 135, 416, 421, 605, 690, 756
Alw26I GTCTC 2 cut(s) 28, 347
AlwI GGATC 1 cut(s) 49
AoxI GGCC 3 cut(s) 219, 278, 463
ApeKI GCWGC 2 cut(s) 578, 605
ApoI RAATTY 1 cut(s) 203
AsiGI ACCGGT 1 cut(s) 197
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 2 cut(s) 310, 463
AsuHPI GGTGA 2 cut(s) 187, 212
AvaII GGWCC 1 cut(s) 310
BaeI ACNNNNGTAYC 2 cut(s) 135, 168
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 565, 592
BccI CCATC 2 cut(s) 250, 524
BceAI ACGGC 4 cut(s) 265, 372, 450, 481
BcoDI GTCTC 2 cut(s) 28, 347
BfmI CTRYAG 1 cut(s) 525
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 5 cut(s) 278, 297, 390, 579, 606
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 5 cut(s) 279, 298, 391, 580, 607
BmcAI AGTACT 2 cut(s) 127, 567
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 310, 463
BmiI GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
BmrI ACTGGG 2 cut(s) 46, 85
BmsI GCATC 4 cut(s) 73, 154, 355, 777
BmuI ACTGGG 2 cut(s) 46, 85
BplI GAGNNNNNCTC 2 cut(s) 324, 356
Bpu10I CCTNAGC 1 cut(s) 806
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 594
Bsa29I ATCGAT 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 3 cut(s) 251, 281, 762
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 41, 91, 573
BseCI ATCGAT 1 cut(s) 695
BseDI CCNNGG 3 cut(s) 251, 281, 762
BseGI GGATG 2 cut(s) 333, 370
BseMII CTCAG 5 cut(s) 45, 134, 138, 651, 820
BseNI ACTGG 3 cut(s) 41, 91, 573
BseRI GAGGAG 7 cut(s) 224, 227, 279, 286, 565, 568, 711
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 565, 592
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 4 cut(s) 239, 296, 490, 707
Bsh1285I CGRYCG 2 cut(s) 281, 385
BshFI GGCC 3 cut(s) 221, 280, 465
BshNI GGYRCC 1 cut(s) 247
BshTI ACCGGT 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 695
BsiEI CGRYCG 2 cut(s) 281, 385
BsiSI CCGG 2 cut(s) 198, 369
BslFI GGGAC 1 cut(s) 323
BsmAI GTCTC 2 cut(s) 28, 347
BsmBI CGTCTC 2 cut(s) 28, 347
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 3 cut(s) 221, 280, 465
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 417
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 8 cut(s) 167, 277, 296, 299, 359, 390, 490, 705
BspANI GGCC 3 cut(s) 221, 280, 465
BspCNI CTCAG 5 cut(s) 44, 133, 139, 650, 819
BspDI ATCGAT 1 cut(s) 695
BspFNI CGCG 4 cut(s) 239, 296, 490, 707
BspLI GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 41, 91, 573
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 3 cut(s) 251, 281, 762
BssMI GATC 1 cut(s) 54
BssNI GRCGYC 1 cut(s) 518
BssT1I CCWWGG 1 cut(s) 762
Bst4CI ACNGT 1 cut(s) 161
BstACI GRCGYC 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 446, 483, 536
BstDEI CTNAG 6 cut(s) 31, 120, 147, 417, 637, 806
BstDSI CCRYGG 2 cut(s) 281, 762
BstF5I GGATG 2 cut(s) 333, 370
BstFNI CGCG 4 cut(s) 239, 296, 490, 707
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 2 cut(s) 28, 347
BstMBI GATC 1 cut(s) 54
BstMCI CGRYCG 2 cut(s) 281, 385
BstMWI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 587
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 4 cut(s) 239, 296, 490, 707
BstV1I GCAGC 2 cut(s) 565, 592
BstZI CGGCCG 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 695
BsuRI GGCC 3 cut(s) 221, 280, 465
BsuTUI ATCGAT 1 cut(s) 695
BtgI CCRYGG 2 cut(s) 281, 762
BtsCI GGATG 2 cut(s) 333, 370
BtsIMutI CAGTG 2 cut(s) 144, 166
Cac8I GCNNGC 3 cut(s) 446, 483, 536
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 310, 463
ClaI ATCGAT 1 cut(s) 695
CseI GACGC 1 cut(s) 10
Csp6I GTAC 6 cut(s) 126, 292, 566, 702, 717, 734
CspAI ACCGGT 1 cut(s) 197
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 3 cut(s) 449, 620, 763
CviQI GTAC 6 cut(s) 126, 292, 566, 702, 717, 734
DdeI CTNAG 6 cut(s) 31, 120, 147, 417, 637, 806
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
EaeI YGGCCR 2 cut(s) 219, 278
EagI CGGCCG 1 cut(s) 278
EciI GGCGGA 2 cut(s) 314, 374
EclXI CGGCCG 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 678
Eco47I GGWCC 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 310
EcoRV GATATC 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 762
Esp3I CGTCTC 2 cut(s) 28, 347
FaeI CATG 3 cut(s) 452, 623, 766
FaiI YATR 9 cut(s) 8, 450, 455, 621, 674, 739, 764, 821, 844
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 410, 442
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 448, 619, 762
FauI CCCGC 1 cut(s) 483
Fnu4HI GCNGC 5 cut(s) 278, 297, 390, 579, 606
FokI GGATG 2 cut(s) 340, 377
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 5 cut(s) 278, 297, 390, 579, 606
GlaI GCGC 1 cut(s) 240
GluI GCNGC 5 cut(s) 278, 297, 390, 579, 606
HaeIII GGCC 3 cut(s) 221, 280, 465
HapII CCGG 2 cut(s) 198, 369
HgaI GACGC 1 cut(s) 10
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 3 cut(s) 452, 623, 766
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HindIII AAGCTT 2 cut(s) 419, 754
HinfI GANTC 9 cut(s) 50, 80, 110, 572, 635, 697, 728, 770, 776
HpaII CCGG 2 cut(s) 198, 369
HphI GGTGA 2 cut(s) 187, 212
Hpy166II GTNNAC 2 cut(s) 230, 634
Hpy188I TCNGA 6 cut(s) 34, 85, 123, 344, 775, 786
Hpy188III TCNNGA 5 cut(s) 25, 71, 611, 725, 808
Hpy8I GTNNAC 2 cut(s) 230, 634
Hpy99I CGWCG 3 cut(s) 80, 520, 523
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 3 cut(s) 581, 741, 790
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 587
HpyF3I CTNAG 6 cut(s) 31, 120, 147, 417, 637, 806
HpySE526I ACGT 1 cut(s) 518
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 3 cut(s) 452, 623, 766
HspAI GCGC 1 cut(s) 239
KflI GGGWCCC 1 cut(s) 310
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 2 cut(s) 413, 493
Lsp1109I GCAGC 2 cut(s) 565, 592
LweI GCATC 4 cut(s) 73, 154, 355, 777
MaeII ACGT 1 cut(s) 518
MaeIII GTNAC 1 cut(s) 640
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 5 cut(s) 59, 328, 331, 771, 808
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 3 cut(s) 203, 746, 813
MlyI GAGTC 4 cut(s) 59, 566, 629, 722
MmeI TCCRAC 1 cut(s) 576
MseI TTAA 1 cut(s) 423
MspI CCGG 2 cut(s) 198, 369
Mva1269I GAATGC 1 cut(s) 191
MvnI CGCG 4 cut(s) 239, 296, 490, 707
MwoI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 587
NcoI CCATGG 1 cut(s) 762
NdeII GATC 1 cut(s) 54
NlaIII CATG 3 cut(s) 452, 623, 766
NlaIV GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
NmeAIII GCCGAG 2 cut(s) 247, 554
NmuCI GTSAC 1 cut(s) 640
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 348, 494, 581
PctI GAATGC 1 cut(s) 191
PfeI GAWTC 5 cut(s) 80, 110, 697, 770, 776
PflFI GACNNNGTC 2 cut(s) 520, 638
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 5 cut(s) 279, 298, 391, 580, 607
PleI GAGTC 4 cut(s) 58, 566, 629, 722
PpsI GAGTC 4 cut(s) 58, 566, 629, 722
PpuMI RGGWCCY 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 310
PspN4I GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
PspPI GGNCC 2 cut(s) 310, 463
PspPPI RGGWCCY 1 cut(s) 310
PsyI GACNNNGTC 2 cut(s) 520, 638
RsaI GTAC 6 cut(s) 127, 293, 567, 703, 718, 735
RsaNI GTAC 6 cut(s) 126, 292, 566, 702, 717, 734
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 5 cut(s) 278, 297, 390, 579, 606
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 310, 463
ScaI AGTACT 2 cut(s) 127, 567
SchI GAGTC 4 cut(s) 59, 566, 629, 722
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 4 cut(s) 73, 154, 355, 777
SfcI CTRYAG 1 cut(s) 525
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 609
SmoI CTYRAG 1 cut(s) 609
Sse9I AATT 3 cut(s) 203, 746, 813
SsiI CCGC 8 cut(s) 167, 277, 296, 299, 359, 390, 490, 705
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 1 cut(s) 521
TaqI TCGA 6 cut(s) 26, 53, 155, 680, 695, 726
TaqII GACCGA 1 cut(s) 399
TasI AATT 3 cut(s) 203, 746, 813
TatI WGTACW 2 cut(s) 125, 565
TauI GCSGC 3 cut(s) 280, 299, 392
TfiI GAWTC 5 cut(s) 80, 110, 697, 770, 776
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 2 cut(s) 151, 166
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 2 cut(s) 578, 605
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 1 cut(s) 809
TspGWI ACGGA 2 cut(s) 47, 495
TspRI CASTG 2 cut(s) 151, 166
Tth111I GACNNNGTC 2 cut(s) 520, 638
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 203
ZraI GACGTC 1 cut(s) 519
ZrmI AGTACT 2 cut(s) 127, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.