Rroxscaffold_4G00307530

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004245
Physical Location & Seq
Reverse (-)
28821853 .. 28823080
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307530.1

Sequence Viewer

Length: 705 bp
ATGTCCACAGAAACTGAAAGCTTCTCAAACTCGAACTGGGCTCTCTTGGAATCCATCCGCCAACATCTTCTAGAGGACGATTTTGAATTTGATACCCAGTCGGCATTTTTCGCAGACTCTCCTTCTCAAAGTACTTCAAGCTCTAGCAGCATTGAAATATCTAGCAACGACTTGGAGATCATGGAGGAGCCGAACCAAGTGTTCACGTGTAAACTGGATGTGGAGGTGGCGGAGCGTGAACACAACGCGCCGTCGAGGGAGAGGCATTTTAGGGGTGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATGAGGGACCCGAAAAAGAACGGGACCAGAGTGTGGCTCGGAACCTATGAGAGGGCGGAGGACGCAGGCTTGGCTTACGACCGAGCTGCTTTCAAACTGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCAAGCATGATATGGAAGTGGGCCGTGTCCTTCCCAAGCGTGCCTCGCCGGAGCTGTCATCTCCGTCGTCAGAAGATGGCTCGCCGAAGCCTAAGAGGAGGAGGAGTGGAGTGGACTCGACAGCCGCGAAAGCCAAGTTGGGTGAAGCTGCTCAAGAGTTTCATGTGTTCCAAGTGGACTCGTTCACATTGGGCGACGAAGATATGGTGCTGTTGTCGCCATCTGAATGCTCGGACATTCTTTCCTTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

234

Amino Acids

26.25

Weight (kDa)

5.37

Isoelectric Point (pI)

66.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 89 - 140 7.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 345
AccII CGCG 3 cut(s) 248, 305, 578
AciI CCGC 7 cut(s) 58, 230, 286, 305, 308, 368, 576
AcoI YGGCCR 1 cut(s) 287
AcsI RAATTY 1 cut(s) 86
AcvI CACGTG 1 cut(s) 207
AfaI GTAC 2 cut(s) 133, 302
AfiI CCNNNNNNNGG 2 cut(s) 345, 363
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 4 cut(s) 86, 138, 155, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 7 cut(s) 21, 141, 398, 425, 430, 505, 599
AluI AGCT 7 cut(s) 21, 141, 398, 425, 430, 505, 599
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 2 cut(s) 287, 472
ApeKI GCWGC 3 cut(s) 147, 398, 599
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 3 cut(s) 319, 336, 472
AsuHPI GGTGA 1 cut(s) 605
AvaII GGWCC 2 cut(s) 319, 336
BanII GRGCYC 1 cut(s) 43
BbrPI CACGTG 1 cut(s) 207
BbvI GCAGC 3 cut(s) 159, 385, 586
BccI CCATC 3 cut(s) 62, 521, 679
BceAI ACGGC 3 cut(s) 235, 274, 459
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BfaI CTAG 3 cut(s) 71, 144, 162
BisI GCNGC 6 cut(s) 148, 287, 306, 399, 576, 600
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 6 cut(s) 149, 288, 307, 400, 577, 601
BmcAI AGTACT 1 cut(s) 133
Bme18I GGWCC 2 cut(s) 319, 336
BmgT120I GGNCC 3 cut(s) 319, 336, 472
BmiI GGNNCC 6 cut(s) 189, 320, 321, 337, 355, 418
BmrI ACTGGG 2 cut(s) 46, 91
BmuI ACTGGG 2 cut(s) 46, 91
BplI GAGNNNNNCTC 2 cut(s) 333, 365
Bpu1102I GCTNAGC 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 588
BsaAI YACGTR 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 541, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 363
Bse1I ACTGG 3 cut(s) 41, 97, 219
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 54, 223
BseLI CCNNNNNNNGG 2 cut(s) 345, 363
BseNI ACTGG 3 cut(s) 41, 97, 219
BseRI GAGGAG 5 cut(s) 200, 295, 562, 565, 568
BseX3I CGGCCG 1 cut(s) 287
BseXI GCAGC 3 cut(s) 159, 385, 586
Bsh1236I CGCG 3 cut(s) 248, 305, 578
Bsh1285I CGRYCG 2 cut(s) 290, 394
BshFI GGCC 2 cut(s) 289, 474
BsiEI CGRYCG 2 cut(s) 290, 394
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 2 cut(s) 332, 349
BslI CCNNNNNNNGG 2 cut(s) 345, 363
BsmFI GGGAC 2 cut(s) 332, 349
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 2 cut(s) 289, 474
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 177
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 7 cut(s) 58, 230, 286, 305, 308, 368, 576
BspANI GGCC 2 cut(s) 289, 474
BspFNI CGCG 3 cut(s) 248, 305, 578
BspLI GGNNCC 6 cut(s) 189, 320, 321, 337, 355, 418
BsrI ACTGG 3 cut(s) 41, 97, 219
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 379, 455, 492, 533
BstDEI CTNAG 2 cut(s) 426, 543
BstDSI CCRYGG 1 cut(s) 290
BstENI CCTNNNNNAGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 54, 223
BstFNI CGCG 3 cut(s) 248, 305, 578
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 1 cut(s) 180
BstMBI GATC 1 cut(s) 177
BstMCI CGRYCG 2 cut(s) 290, 394
BstMWI GCNNNNNNNGC 7 cut(s) 110, 147, 374, 383, 496, 581, 667
BstUI CGCG 3 cut(s) 248, 305, 578
BstV1I GCAGC 3 cut(s) 159, 385, 586
BstZI CGGCCG 1 cut(s) 287
BsuRI GGCC 2 cut(s) 289, 474
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 2 cut(s) 54, 223
Cac8I GCNNGC 4 cut(s) 379, 455, 492, 533
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 319, 336, 472
CseI GACGC 1 cut(s) 383
Csp6I GTAC 2 cut(s) 132, 301
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 3 cut(s) 181, 458, 614
CviQI GTAC 2 cut(s) 132, 301
DdeI CTNAG 2 cut(s) 426, 543
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 287
EagI CGGCCG 1 cut(s) 287
EciI GGCGGA 4 cut(s) 47, 245, 323, 383
EclXI CGGCCG 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 2 cut(s) 319, 336
Eco52I CGGCCG 1 cut(s) 287
Eco72I CACGTG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 43
FaeI CATG 3 cut(s) 184, 461, 617
FaiI YATR 7 cut(s) 182, 360, 459, 464, 615, 656, 703
FalI AAGNNNNNCTT 4 cut(s) 414, 446, 419, 451
FaqI GGGAC 2 cut(s) 332, 349
FatI CATG 3 cut(s) 180, 457, 613
Fnu4HI GCNGC 6 cut(s) 148, 287, 306, 399, 576, 600
FokI GGATG 2 cut(s) 41, 230
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 6 cut(s) 148, 287, 306, 399, 576, 600
FspBI CTAG 3 cut(s) 71, 144, 162
GlaI GCGC 1 cut(s) 249
GluI GCNGC 6 cut(s) 148, 287, 306, 399, 576, 600
HaeIII GGCC 2 cut(s) 289, 474
HapII CCGG 1 cut(s) 500
HgaI GACGC 1 cut(s) 383
HhaI GCGC 1 cut(s) 250
Hin1II CATG 3 cut(s) 184, 461, 617
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HindIII AAGCTT 2 cut(s) 19, 428
HinfI GANTC 4 cut(s) 50, 116, 566, 629
HpaII CCGG 1 cut(s) 500
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 7 cut(s) 6, 204, 212, 239, 565, 628, 636
Hpy188I TCNGA 4 cut(s) 353, 523, 676, 685
Hpy188III TCNNGA 2 cut(s) 71, 605
Hpy8I GTNNAC 7 cut(s) 6, 204, 212, 239, 565, 628, 636
Hpy99I CGWCG 3 cut(s) 256, 520, 650
HpyAV CCTTC 2 cut(s) 132, 491
HpyCH4IV ACGT 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 147, 374, 383, 496, 581, 667
HpyF3I CTNAG 2 cut(s) 426, 543
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 3 cut(s) 184, 461, 617
HspAI GCGC 1 cut(s) 248
KflI GGGWCCC 1 cut(s) 319
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 4 cut(s) 187, 232, 422, 502
LpnPI CCDG 6 cut(s) 22, 110, 200, 352, 363, 513
Lsp1109I GCAGC 3 cut(s) 159, 385, 586
MaeI CTAG 3 cut(s) 71, 144, 162
MaeII ACGT 1 cut(s) 206
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 3 cut(s) 59, 536, 662
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 1 cut(s) 86
MlyI GAGTC 3 cut(s) 110, 560, 623
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 676
MspI CCGG 1 cut(s) 500
Mva1269I GAATGC 1 cut(s) 683
MvnI CGCG 3 cut(s) 248, 305, 578
MwoI GCNNNNNNNGC 7 cut(s) 110, 147, 374, 383, 496, 581, 667
NdeII GATC 1 cut(s) 177
NlaIII CATG 3 cut(s) 184, 461, 617
NlaIV GGNNCC 6 cut(s) 189, 320, 321, 337, 355, 418
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PctI GAATGC 1 cut(s) 683
PfeI GAWTC 1 cut(s) 50
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 6 cut(s) 149, 288, 307, 400, 577, 601
PleI GAGTC 3 cut(s) 110, 560, 623
PmaCI CACGTG 1 cut(s) 207
PmlI CACGTG 1 cut(s) 207
PpsI GAGTC 3 cut(s) 110, 560, 623
Ppu21I YACGTR 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
PspCI CACGTG 1 cut(s) 207
PspN4I GGNNCC 6 cut(s) 189, 320, 321, 337, 355, 418
PspPI GGNCC 3 cut(s) 319, 336, 472
PspPPI RGGWCCY 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 14
RsaI GTAC 2 cut(s) 133, 302
RsaNI GTAC 2 cut(s) 132, 301
RseI CAYNNNNRTG 1 cut(s) 676
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 6 cut(s) 148, 287, 306, 399, 576, 600
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 3 cut(s) 319, 336, 472
ScaI AGTACT 1 cut(s) 133
SchI GAGTC 3 cut(s) 110, 560, 623
SduI GDGCHC 1 cut(s) 43
SinI GGWCC 2 cut(s) 319, 336
SmiMI CAYNNNNRTG 1 cut(s) 676
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
Sse9I AATT 1 cut(s) 86
SsiI CCGC 7 cut(s) 58, 230, 286, 305, 308, 368, 576
SspMI CTAG 3 cut(s) 71, 144, 162
TaiI ACGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 32, 254, 569
TaqII GACCGA 1 cut(s) 408
TasI AATT 1 cut(s) 86
TatI WGTACW 1 cut(s) 131
TauI GCSGC 3 cut(s) 289, 308, 578
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseI GCWGC 3 cut(s) 147, 398, 599
TspDTI ATGAA 1 cut(s) 602
TspGWI ACGGA 1 cut(s) 504
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 2 cut(s) 319, 336
XagI CCTNNNNNAGG 1 cut(s) 361
XapI RAATTY 1 cut(s) 86
XbaI TCTAGA 1 cut(s) 70
XspI CTAG 3 cut(s) 71, 144, 162
ZrmI AGTACT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.