RLG00000028722

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004245
Physical Location & Seq
Reverse (-)
26984377 .. 26985093
717 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028722

Sequence Viewer

Length: 717 bp
ATGTCCAAAGAAACAAGCTTCTCAAACTCAGACTGGGCTCTCTTGGAATCGATCCGCCAGCATCTTCTCGAAGACGATTTCGATACCCAGTCAGCATTTTCCGCAGACTCTCCAGCTCAAAGTACGTCCAGCTCTAACTGCACTGAGATATCGAACAGTGATGCGGAGATAGTGAAGCAGAACCAAGCATTCACTAGTGAATTTGAGGCGGAGGTGGCGGCGCGTGAACAGCACGCACCATCGGGTCAGAGGCATTTTAGAGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCAGAGATAAGGGACCCGAAAAAGAACGGGACGAGAGTGTGGCTCGGGACCTACGAGACGGCGGAGGACGCAGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCATGCATGATGTGGAGGTGGGCCGTGTGCTTCCCAAGCGTGCCTCGCCGGAGCCATCATCTCCGTCCTCCACGACGTCGTCTGTAGATGGCTCGCCGAAGCCAAAGAGGAGGAGGGGTGGAGTGGGCTCGGCTGCGGCGGAAGCCCAGTTGGAGGAAGCTGCTCAAGAGTTTCATGTGTTCCAAGTGGACTCATTCACATTTTGCAACGAGCTATTGGTTGAGGATATGGCACTCTTGTCACCATCTGAATGCTCAGACATTCTTTCTTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

239

Amino Acids

26.19

Weight (kDa)

5.33

Isoelectric Point (pI)

65.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 85 - 136 1.5e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 518
AccII CGCG 2 cut(s) 223, 293
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 2 cut(s) 124, 290
AfiI CCNNNNNNNGG 1 cut(s) 592
AgsI TTSAA 1 cut(s) 394
AhlI ACTAGT 1 cut(s) 194
AluBI AGCT 7 cut(s) 18, 116, 132, 413, 418, 599, 652
AluI AGCT 7 cut(s) 18, 116, 132, 413, 418, 599, 652
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 2 cut(s) 275, 460
ApeKI GCWGC 2 cut(s) 572, 599
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 307, 342, 460
AsuHPI GGTGA 1 cut(s) 672
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 307, 342
BanII GRGCYC 2 cut(s) 40, 569
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 559, 586
BccI CCATC 4 cut(s) 247, 502, 521, 691
BceAI ACGGC 3 cut(s) 262, 369, 447
BcoDI GTCTC 1 cut(s) 344
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 7 cut(s) 219, 275, 294, 387, 573, 576, 600
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 7 cut(s) 220, 276, 295, 388, 574, 577, 601
Bme18I GGWCC 2 cut(s) 307, 342
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 3 cut(s) 307, 342, 460
BmiI GGNNCC 5 cut(s) 308, 309, 343, 406, 492
BmrI ACTGGG 3 cut(s) 43, 82, 580
BmsI GCATC 2 cut(s) 70, 151
BmuI ACTGGG 3 cut(s) 43, 82, 580
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 321, 353
BpmI CTGGAG 1 cut(s) 96
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 588
Bsa29I ATCGAT 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 541, 571
Bsc4I CCNNNNNNNGG 1 cut(s) 592
Bse1I ACTGG 3 cut(s) 38, 88, 586
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 278
BseLI CCNNNNNNNGG 1 cut(s) 592
BseMII CTCAG 3 cut(s) 42, 135, 708
BseNI ACTGG 3 cut(s) 38, 88, 586
BseRI GAGGAG 4 cut(s) 276, 283, 562, 565
BseX3I CGGCCG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 559, 586
BsgI GTGCAG 1 cut(s) 124
Bsh1236I CGCG 2 cut(s) 223, 293
Bsh1285I CGRYCG 2 cut(s) 278, 382
BshFI GGCC 2 cut(s) 277, 462
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 2 cut(s) 278, 382
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 3 cut(s) 320, 337, 355
BslI CCNNNNNNNGG 1 cut(s) 592
BsmAI GTCTC 1 cut(s) 344
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 3 cut(s) 320, 337, 355
BsmI GAATGC 2 cut(s) 188, 695
BsnI GGCC 2 cut(s) 277, 462
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 40, 569
Bsp143I GATC 1 cut(s) 51
Bsp1720I GCTNAGC 1 cut(s) 414
BspANI GGCC 2 cut(s) 277, 462
BspCNI CTCAG 3 cut(s) 41, 136, 707
BspDI ATCGAT 1 cut(s) 50
BspFNI CGCG 2 cut(s) 223, 293
BspLI GGNNCC 5 cut(s) 308, 309, 343, 406, 492
BspPI GGATC 1 cut(s) 46
BsrI ACTGG 3 cut(s) 38, 88, 586
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 515
BstC8I GCNNGC 5 cut(s) 59, 234, 443, 480, 533
BstDEI CTNAG 4 cut(s) 28, 144, 414, 694
BstDSI CCRYGG 1 cut(s) 278
BstFNI CGCG 2 cut(s) 223, 293
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 2 cut(s) 278, 382
BstMWI GCNNNNNNNGC 9 cut(s) 101, 138, 215, 229, 362, 371, 475, 484, 581
BstNSI RCATGY 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 522
BstUI CGCG 2 cut(s) 223, 293
BstV1I GCAGC 2 cut(s) 559, 586
BstV2I GAAGAC 1 cut(s) 78
BstZI CGGCCG 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 2 cut(s) 277, 462
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 141, 163
Cac8I GCNNGC 5 cut(s) 59, 234, 443, 480, 533
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 3 cut(s) 307, 342, 460
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 1 cut(s) 371
Csp6I GTAC 2 cut(s) 123, 289
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 3 cut(s) 442, 446, 614
CviQI GTAC 2 cut(s) 123, 289
DdeI CTNAG 4 cut(s) 28, 144, 414, 694
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 275
EagI CGGCCG 1 cut(s) 275
EciI GGCGGA 4 cut(s) 44, 224, 371, 593
EclXI CGGCCG 1 cut(s) 275
Eco24I GRGCYC 2 cut(s) 40, 569
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 307, 342
Eco52I CGGCCG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 2 cut(s) 307, 342
EcoRV GATATC 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 2 cut(s) 40, 569
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 3 cut(s) 445, 449, 617
FaiI YATR 4 cut(s) 443, 447, 615, 668
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 407, 439
FaqI GGGAC 3 cut(s) 320, 337, 355
FatI CATG 3 cut(s) 441, 445, 613
Fnu4HI GCNGC 7 cut(s) 219, 275, 294, 387, 573, 576, 600
FriOI GRGCYC 2 cut(s) 40, 569
Fsp4HI GCNGC 7 cut(s) 219, 275, 294, 387, 573, 576, 600
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 222
GluI GCNGC 7 cut(s) 219, 275, 294, 387, 573, 576, 600
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 277, 462
HapII CCGG 1 cut(s) 488
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 3 cut(s) 445, 449, 617
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HindIII AAGCTT 2 cut(s) 16, 416
HinfI GANTC 3 cut(s) 47, 107, 629
HpaII CCGG 1 cut(s) 488
HphI GGTGA 1 cut(s) 672
Hpy166II GTNNAC 2 cut(s) 227, 628
Hpy188I TCNGA 4 cut(s) 31, 249, 688, 697
Hpy188III TCNNGA 3 cut(s) 68, 340, 605
Hpy8I GTNNAC 2 cut(s) 227, 628
Hpy99I CGWCG 2 cut(s) 517, 520
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 125, 515
HpyCH4V TGCA 3 cut(s) 141, 445, 645
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 138, 215, 229, 362, 371, 475, 484, 581
HpyF3I CTNAG 4 cut(s) 28, 144, 414, 694
HpySE526I ACGT 2 cut(s) 125, 515
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 3 cut(s) 445, 449, 617
HspAI GCGC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 307
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 410, 490
LpnPI CCDG 8 cut(s) 19, 71, 101, 126, 142, 351, 501, 599
Lsp1109I GCAGC 2 cut(s) 559, 586
LweI GCATC 2 cut(s) 70, 151
MaeI CTAG 1 cut(s) 195
MaeII ACGT 2 cut(s) 125, 515
MaeIII GTNAC 1 cut(s) 678
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 2 cut(s) 56, 83
MhlI GDGCHC 2 cut(s) 40, 569
MluCI AATT 1 cut(s) 200
MlyI GAGTC 2 cut(s) 101, 623
MmeI TCCRAC 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 447
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 688
MspI CCGG 1 cut(s) 488
Mva1269I GAATGC 2 cut(s) 188, 695
MvnI CGCG 2 cut(s) 223, 293
MwoI GCNNNNNNNGC 9 cut(s) 101, 138, 215, 229, 362, 371, 475, 484, 581
NdeII GATC 1 cut(s) 51
NlaIII CATG 3 cut(s) 445, 449, 617
NlaIV GGNNCC 5 cut(s) 308, 309, 343, 406, 492
NmeAIII GCCGAG 1 cut(s) 548
NmuCI GTSAC 1 cut(s) 678
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 1 cut(s) 445
PaeI GCATGC 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 2 cut(s) 188, 695
PfeI GAWTC 1 cut(s) 47
PflFI GACNNNGTC 1 cut(s) 517
PkrI GCNGC 7 cut(s) 220, 276, 295, 388, 574, 577, 601
PleI GAGTC 2 cut(s) 101, 623
PpsI GAGTC 2 cut(s) 101, 623
PpuMI RGGWCCY 2 cut(s) 307, 342
Psp5II RGGWCCY 2 cut(s) 307, 342
PspN4I GGNNCC 5 cut(s) 308, 309, 343, 406, 492
PspPI GGNCC 3 cut(s) 307, 342, 460
PspPPI RGGWCCY 2 cut(s) 307, 342
PsyI GACNNNGTC 1 cut(s) 517
RsaI GTAC 2 cut(s) 124, 290
RsaNI GTAC 2 cut(s) 123, 289
RseI CAYNNNNRTG 1 cut(s) 688
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 7 cut(s) 219, 275, 294, 387, 573, 576, 600
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 3 cut(s) 307, 342, 460
SchI GAGTC 2 cut(s) 101, 623
SduI GDGCHC 2 cut(s) 40, 569
SfaNI GCATC 2 cut(s) 70, 151
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 2 cut(s) 307, 342
SmiMI CAYNNNNRTG 1 cut(s) 688
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
SpeI ACTAGT 1 cut(s) 194
SphI GCATGC 1 cut(s) 445
Sse9I AATT 1 cut(s) 200
SspMI CTAG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 128, 518
TaqI TCGA 4 cut(s) 50, 69, 81, 152
TaqII GACCGA 1 cut(s) 396
TasI AATT 1 cut(s) 200
TauI GCSGC 5 cut(s) 221, 277, 296, 389, 578
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 2 cut(s) 148, 163
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 2 cut(s) 572, 599
Tsp45I GTSAC 1 cut(s) 678
TspDTI ATGAA 1 cut(s) 602
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 2 cut(s) 148, 163
Tth111I GACNNNGTC 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 307, 342
XapI RAATTY 1 cut(s) 200
XceI RCATGY 1 cut(s) 445
XspI CTAG 1 cut(s) 195
ZraI GACGTC 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.