Rw1G017250

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004245
Physical Location & Seq
Forward (+)
38244205 .. 38245045
841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017250.1

Sequence Viewer

Length: 780 bp
ATGGAAGGCCTGATAATCCCCATTAACACACAGCTTCAATATCCACTCTTAAGACTTCCAAAACTCACAGCTTCAAACTTCTCGAACTCATACTGGCGGGCTCTCTTGGAGTCGATCCGTCAGCATCTTCTTGACGACGATTTCGATACCCAGTTGGGTTTTTTCGCAGATTCTCCTGCTCAACGTACTTCCAGCTCTAACAGCACTGAGATATCGAACAGTGATGCGGAGATAGTGGGGCGGAACCAAGCATTCACTAGTGAATTTGAGGAGGAGGCGGCGGAGCGTAAACAGCACGCGCCATCGGCACCGAGGCATTTTAGGGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAGAAGAACGGGATGAGAGTGTGGCTCGGAACCTACGAGACGGCGGAGGATGCCGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTCAACTTTCCTCACTTGATTGGCACGCATGATATGGAGGTGGGCCGTGTGTTTCCCAAGCGTGCCTCGCCGGAGCCGTCATCTCCGTCCTCCACGACGTCGTCTGAAGATGGCTCGCCGAAGCCAAAGAGGAGGAGGGGTGGAGTGGACTCGGCTGCGGCGAAAGCCAAGTTGGATGAAGGTGCTCGAGAGTTTCATCTGTTCCAAGTGGACTCACTCACATTTGGCAACGAATTATTGGTTGAGGATATGGTGCTTTTGTCACCATCTGAATGCTCAGACATTCTTTCTTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

259

Amino Acids

28.91

Weight (kDa)

6.33

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 106 - 157 9.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 307
AccII CGCG 2 cut(s) 299, 356
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 338
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 1 cut(s) 578
AfaI GTAC 2 cut(s) 187, 353
AflII CTTAAG 1 cut(s) 49
AgsI TTSAA 3 cut(s) 38, 75, 457
AhlI ACTAGT 1 cut(s) 257
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AluBI AGCT 5 cut(s) 34, 71, 195, 476, 481
AluI AGCT 5 cut(s) 34, 71, 195, 476, 481
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 666
AoxI GGCC 3 cut(s) 7, 338, 523
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 263
ArsI GACNNNNNNTTYG 2 cut(s) 125, 157
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 2 cut(s) 370, 523
AsuHPI GGTGA 1 cut(s) 735
AvaI CYCGRG 1 cut(s) 666
AvaII GGWCC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 622
BccI CCATC 3 cut(s) 310, 584, 754
BceAI ACGGC 4 cut(s) 325, 432, 510, 541
BcoDI GTCTC 1 cut(s) 407
BcuI ACTAGT 1 cut(s) 257
BfaI CTAG 1 cut(s) 258
BfrI CTTAAG 1 cut(s) 49
BglI GCCNNNNNGGC 1 cut(s) 434
BisI GCNGC 6 cut(s) 279, 338, 357, 450, 636, 639
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 6 cut(s) 280, 339, 358, 451, 637, 640
Bme18I GGWCC 1 cut(s) 370
BmeT110I CYCGRG 1 cut(s) 666
BmgT120I GGNCC 2 cut(s) 370, 523
BmiI GGNNCC 7 cut(s) 245, 309, 371, 372, 406, 469, 555
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 3 cut(s) 133, 214, 415
BmuI ACTGGG 1 cut(s) 145
BplI GAGNNNNNCTC 2 cut(s) 384, 416
Bpu1102I GCTNAGC 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 578
BsaJI CCNNGG 2 cut(s) 311, 341
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bse1I ACTGG 2 cut(s) 98, 151
BseDI CCNNGG 2 cut(s) 311, 341
BseGI GGATG 3 cut(s) 393, 430, 661
BseMII CTCAG 2 cut(s) 198, 771
BseNI ACTGG 2 cut(s) 98, 151
BseRI GAGGAG 5 cut(s) 284, 287, 346, 625, 628
BseX3I CGGCCG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 622
Bsh1236I CGCG 2 cut(s) 299, 356
Bsh1285I CGRYCG 2 cut(s) 341, 445
BshFI GGCC 3 cut(s) 9, 340, 525
BshNI GGYRCC 1 cut(s) 307
BsiEI CGRYCG 2 cut(s) 341, 445
BsiHKAI GWGCWC 1 cut(s) 667
BsiHKCI CYCGRG 1 cut(s) 666
BsiSI CCGG 2 cut(s) 429, 551
BslFI GGGAC 1 cut(s) 383
BsmAI GTCTC 1 cut(s) 407
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 2 cut(s) 251, 758
BsnI GGCC 3 cut(s) 9, 340, 525
BsoBI CYCGRG 1 cut(s) 666
Bsp1286I GDGCHC 2 cut(s) 103, 667
Bsp143I GATC 1 cut(s) 114
Bsp1720I GCTNAGC 1 cut(s) 477
BspANI GGCC 3 cut(s) 9, 340, 525
BspCNI CTCAG 2 cut(s) 199, 770
BspFNI CGCG 2 cut(s) 299, 356
BspLI GGNNCC 7 cut(s) 245, 309, 371, 372, 406, 469, 555
BspPI GGATC 1 cut(s) 109
BspT107I GGYRCC 1 cut(s) 307
BspTI CTTAAG 1 cut(s) 49
BsrI ACTGG 2 cut(s) 98, 151
BssECI CCNNGG 2 cut(s) 311, 341
BssMI GATC 1 cut(s) 114
BssNI GRCGYC 1 cut(s) 578
Bst4CI ACNGT 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 578
BstAFI CTTAAG 1 cut(s) 49
BstC8I GCNNGC 5 cut(s) 99, 297, 506, 543, 596
BstDEI CTNAG 3 cut(s) 207, 477, 757
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 3 cut(s) 393, 430, 661
BstFNI CGCG 2 cut(s) 299, 356
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 1 cut(s) 114
BstMCI CGRYCG 2 cut(s) 341, 445
BstMWI GCNNNNNNNGC 7 cut(s) 201, 292, 305, 425, 434, 547, 644
BstUI CGCG 2 cut(s) 299, 356
BstV1I GCAGC 1 cut(s) 622
BstZI CGGCCG 1 cut(s) 338
BsuRI GGCC 3 cut(s) 9, 340, 525
BtgI CCRYGG 1 cut(s) 341
BtsCI GGATG 3 cut(s) 393, 430, 661
BtsIMutI CAGTG 2 cut(s) 204, 226
Cac8I GCNNGC 5 cut(s) 99, 297, 506, 543, 596
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 2 cut(s) 370, 523
Csp6I GTAC 2 cut(s) 186, 352
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviAII CATG 1 cut(s) 509
CviQI GTAC 2 cut(s) 186, 352
DdeI CTNAG 3 cut(s) 207, 477, 757
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 338
EagI CGGCCG 1 cut(s) 338
EciI GGCGGA 4 cut(s) 256, 296, 374, 434
EclXI CGGCCG 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 103
Eco32I GATATC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 370
Eco52I CGGCCG 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 666
EcoO109I RGGNCCY 1 cut(s) 370
EcoRV GATATC 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 103
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 1 cut(s) 512
FaiI YATR 4 cut(s) 91, 510, 515, 731
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FaqI GGGAC 1 cut(s) 383
FatI CATG 1 cut(s) 508
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 6 cut(s) 279, 338, 357, 450, 636, 639
FokI GGATG 3 cut(s) 400, 437, 668
FriOI GRGCYC 1 cut(s) 103
Fsp4HI GCNGC 6 cut(s) 279, 338, 357, 450, 636, 639
FspBI CTAG 1 cut(s) 258
GlaI GCGC 1 cut(s) 300
GluI GCNGC 6 cut(s) 279, 338, 357, 450, 636, 639
HaeIII GGCC 3 cut(s) 9, 340, 525
HapII CCGG 2 cut(s) 429, 551
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 1 cut(s) 578
Hin1II CATG 1 cut(s) 512
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HinfI GANTC 4 cut(s) 110, 170, 629, 692
HpaII CCGG 2 cut(s) 429, 551
HphI GGTGA 1 cut(s) 735
Hpy166II GTNNAC 3 cut(s) 290, 628, 691
Hpy188I TCNGA 4 cut(s) 404, 586, 751, 760
Hpy188III TCNNGA 3 cut(s) 82, 131, 668
Hpy8I GTNNAC 3 cut(s) 290, 628, 691
Hpy99I CGWCG 3 cut(s) 140, 580, 583
HpyAV CCTTC 1 cut(s) 653
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 2 cut(s) 184, 578
HpyF10VI GCNNNNNNNGC 7 cut(s) 201, 292, 305, 425, 434, 547, 644
HpyF3I CTNAG 3 cut(s) 207, 477, 757
HpySE526I ACGT 2 cut(s) 184, 578
Hsp92I GRCGYC 1 cut(s) 578
Hsp92II CATG 1 cut(s) 512
HspAI GCGC 1 cut(s) 299
KflI GGGWCCC 1 cut(s) 370
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 3 cut(s) 283, 473, 553
LpnPI CCDG 7 cut(s) 23, 79, 164, 189, 205, 442, 564
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 3 cut(s) 133, 214, 415
MaeI CTAG 1 cut(s) 258
MaeII ACGT 2 cut(s) 184, 578
MaeIII GTNAC 1 cut(s) 741
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 4 cut(s) 119, 388, 391, 599
MhlI GDGCHC 2 cut(s) 103, 667
MluCI AATT 2 cut(s) 263, 713
MlyI GAGTC 3 cut(s) 119, 623, 686
MmeI TCCRAC 1 cut(s) 633
MseI TTAA 2 cut(s) 24, 50
MslI CAYNNNNRTG 1 cut(s) 751
MspCI CTTAAG 1 cut(s) 49
MspI CCGG 2 cut(s) 429, 551
Mva1269I GAATGC 2 cut(s) 251, 758
MvnI CGCG 2 cut(s) 299, 356
MwoI GCNNNNNNNGC 7 cut(s) 201, 292, 305, 425, 434, 547, 644
NdeII GATC 1 cut(s) 114
NlaIII CATG 1 cut(s) 512
NlaIV GGNNCC 7 cut(s) 245, 309, 371, 372, 406, 469, 555
NmeAIII GCCGAG 1 cut(s) 611
NmuCI GTSAC 1 cut(s) 741
PaeR7I CTCGAG 1 cut(s) 666
PceI AGGCCT 1 cut(s) 9
PcsI WCGNNNNNNNCGW 4 cut(s) 141, 408, 554, 638
PctI GAATGC 2 cut(s) 251, 758
PfeI GAWTC 1 cut(s) 170
PflFI GACNNNGTC 1 cut(s) 580
PkrI GCNGC 6 cut(s) 280, 339, 358, 451, 637, 640
PleI GAGTC 3 cut(s) 118, 623, 686
PpsI GAGTC 3 cut(s) 118, 623, 686
PpuMI RGGWCCY 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 370
PspN4I GGNNCC 7 cut(s) 245, 309, 371, 372, 406, 469, 555
PspPI GGNCC 2 cut(s) 370, 523
PspPPI RGGWCCY 1 cut(s) 370
PsyI GACNNNGTC 1 cut(s) 580
RsaI GTAC 2 cut(s) 187, 353
RsaNI GTAC 2 cut(s) 186, 352
RseI CAYNNNNRTG 1 cut(s) 751
SaqAI TTAA 2 cut(s) 24, 50
SatI GCNGC 6 cut(s) 279, 338, 357, 450, 636, 639
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 2 cut(s) 370, 523
SchI GAGTC 3 cut(s) 119, 623, 686
SduI GDGCHC 2 cut(s) 103, 667
SfaNI GCATC 3 cut(s) 133, 214, 415
Sfr274I CTCGAG 1 cut(s) 666
SinI GGWCC 1 cut(s) 370
SlaI CTCGAG 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 751
SmlI CTYRAG 2 cut(s) 49, 666
SmoI CTYRAG 2 cut(s) 49, 666
SpeI ACTAGT 1 cut(s) 257
Sse9I AATT 2 cut(s) 263, 713
SseBI AGGCCT 1 cut(s) 9
SspMI CTAG 1 cut(s) 258
StuI AGGCCT 1 cut(s) 9
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 2 cut(s) 187, 581
TaqI TCGA 5 cut(s) 83, 113, 144, 215, 667
TaqII GACCGA 1 cut(s) 459
TasI AATT 2 cut(s) 263, 713
TauI GCSGC 5 cut(s) 281, 340, 359, 452, 641
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 24, 50
Tru9I TTAA 2 cut(s) 24, 50
TscAI CASTG 2 cut(s) 211, 226
TseFI GTSAC 1 cut(s) 741
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 1 cut(s) 741
TspDTI ATGAA 2 cut(s) 665, 672
TspGWI ACGGA 2 cut(s) 107, 555
TspRI CASTG 2 cut(s) 211, 226
Tth111I GACNNNGTC 1 cut(s) 580
Vha464I CTTAAG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 666
XspI CTAG 1 cut(s) 258
ZraI GACGTC 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.