Rh1BG171800

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004245
Physical Location & Seq
Forward (+)
27397618 .. 27398325
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG171800.1

Sequence Viewer

Length: 708 bp
ATGTCCACAGAAACTGAAAGCTTCTCAAACTCGAACTGGGCTCTCTTGGAATCCATCCGCCAACATCTTCTAGACGACGATTTTGAATTTGATACCCAGTCGGCATTTTTTGCAAACTCTCCTGCTCAAAGTACTTCAAGCTCTAGCAGAATTGAAATATCGAGCAACGACTTGGAGATCGTGGAGGACCCGAACCAAGTGTTCACGTGTAAACTGGAGGTGGAGGTGGCGGAGCGTGAACACAACACGCCGTCGAGGGAGAGGCATAATTTTAGGGGCGTGAGGAGGCGGCCATGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAAAAGAACGGGACCAGAGTGTGGCTCGGTACCTATGAGAGGGCGGAGGACGCAGGCTTGGCTTACGACCGAGCTGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCACGCATGATATGGAAGTGGGCCGTGTCCTTCCCAAGCGTGCCTCGCCGGAGCTGTCATCTCCGTCGTCAGAAGATGGCTCGCCGAAGCCTAAGAGGAGGAGGAGTGGAGTGGACTCGACAGCCGCGAAAGCCAAGTTGGGTGAAGCTGCTCAAGAGTTTCATGTGTTCCAAGTGGACTCGTTCACATTTGGCGACGAAGATATGGTGCTGTTGTCGCCATCTGAATGCTCGGACATTCTTTCCTTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

235

Amino Acids

26.41

Weight (kDa)

5.48

Isoelectric Point (pI)

64.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 91 - 141 4.5e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 1 cut(s) 348
AccII CGCG 2 cut(s) 308, 581
AciI CCGC 7 cut(s) 58, 230, 289, 308, 311, 371, 579
AcoI YGGCCR 1 cut(s) 290
AcsI RAATTY 1 cut(s) 86
AcvI CACGTG 1 cut(s) 207
AfaI GTAC 3 cut(s) 133, 305, 358
AfiI CCNNNNNNNGG 2 cut(s) 348, 366
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 4 cut(s) 86, 138, 155, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 7 cut(s) 21, 141, 401, 428, 433, 508, 602
AluI AGCT 7 cut(s) 21, 141, 401, 428, 433, 508, 602
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 2 cut(s) 290, 475
ApeKI GCWGC 2 cut(s) 401, 602
ApoI RAATTY 1 cut(s) 86
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp718I GGTACC 1 cut(s) 356
AspS9I GGNCC 4 cut(s) 187, 322, 339, 475
AsuHPI GGTGA 1 cut(s) 608
AvaII GGWCC 3 cut(s) 187, 322, 339
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 43
BbrPI CACGTG 1 cut(s) 207
BbvI GCAGC 2 cut(s) 388, 589
BccI CCATC 3 cut(s) 62, 524, 682
BceAI ACGGC 2 cut(s) 235, 462
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BfaI CTAG 2 cut(s) 71, 144
BisI GCNGC 5 cut(s) 290, 309, 402, 579, 603
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 5 cut(s) 291, 310, 403, 580, 604
BmcAI AGTACT 1 cut(s) 133
Bme18I GGWCC 3 cut(s) 187, 322, 339
BmgT120I GGNCC 4 cut(s) 187, 322, 339, 475
BmiI GGNNCC 6 cut(s) 189, 323, 324, 340, 358, 421
BmrI ACTGGG 2 cut(s) 46, 91
BmuI ACTGGG 2 cut(s) 46, 91
BplI GAGNNNNNCTC 2 cut(s) 336, 368
BpmI CTGGAG 1 cut(s) 236
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 591
BsaAI YACGTR 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 366
Bse1I ACTGG 3 cut(s) 41, 97, 219
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 2 cut(s) 348, 366
BseNI ACTGG 3 cut(s) 41, 97, 219
BseRI GAGGAG 4 cut(s) 298, 565, 568, 571
BseXI GCAGC 2 cut(s) 388, 589
Bsh1236I CGCG 2 cut(s) 308, 581
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 292, 477
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 397
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 2 cut(s) 335, 352
BslI CCNNNNNNNGG 2 cut(s) 348, 366
BsmFI GGGAC 2 cut(s) 335, 352
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 2 cut(s) 292, 477
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 177
Bsp1720I GCTNAGC 1 cut(s) 429
Bsp19I CCATGG 1 cut(s) 293
BspACI CCGC 7 cut(s) 58, 230, 289, 308, 311, 371, 579
BspANI GGCC 2 cut(s) 292, 477
BspFNI CGCG 2 cut(s) 308, 581
BspLI GGNNCC 6 cut(s) 189, 323, 324, 340, 358, 421
BspT107I GGYRCC 1 cut(s) 356
BsrI ACTGG 3 cut(s) 41, 97, 219
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 382, 458, 495, 536
BstDEI CTNAG 2 cut(s) 429, 546
BstDSI CCRYGG 1 cut(s) 293
BstENI CCTNNNNNAGG 1 cut(s) 364
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 2 cut(s) 308, 581
BstKTI GATC 1 cut(s) 180
BstMBI GATC 1 cut(s) 177
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 6 cut(s) 110, 377, 386, 499, 584, 670
BstUI CGCG 2 cut(s) 308, 581
BstV1I GCAGC 2 cut(s) 388, 589
BsuRI GGCC 2 cut(s) 292, 477
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 1 cut(s) 54
Cac8I GCNNGC 4 cut(s) 382, 458, 495, 536
CaiI CAGNNNCTG 1 cut(s) 14
Cfr13I GGNCC 4 cut(s) 187, 322, 339, 475
CseI GACGC 1 cut(s) 386
Csp6I GTAC 3 cut(s) 132, 304, 357
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 3 cut(s) 294, 461, 617
CviQI GTAC 3 cut(s) 132, 304, 357
DdeI CTNAG 2 cut(s) 429, 546
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 290
EciI GGCGGA 4 cut(s) 47, 245, 326, 386
Eco130I CCWWGG 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 3 cut(s) 187, 322, 339
Eco72I CACGTG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 364
EcoO109I RGGNCCY 2 cut(s) 187, 322
EcoT14I CCWWGG 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 3 cut(s) 297, 464, 620
FaiI YATR 8 cut(s) 267, 295, 363, 462, 467, 618, 659, 706
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 422, 454
FaqI GGGAC 2 cut(s) 335, 352
FatI CATG 3 cut(s) 293, 460, 616
Fnu4HI GCNGC 5 cut(s) 290, 309, 402, 579, 603
FokI GGATG 1 cut(s) 41
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 5 cut(s) 290, 309, 402, 579, 603
FspBI CTAG 2 cut(s) 71, 144
GluI GCNGC 5 cut(s) 290, 309, 402, 579, 603
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 2 cut(s) 292, 477
HapII CCGG 1 cut(s) 503
HgaI GACGC 1 cut(s) 386
Hin1II CATG 3 cut(s) 297, 464, 620
HindIII AAGCTT 2 cut(s) 19, 431
HinfI GANTC 3 cut(s) 50, 569, 632
HpaII CCGG 1 cut(s) 503
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 7 cut(s) 6, 204, 212, 239, 568, 631, 639
Hpy188I TCNGA 3 cut(s) 526, 679, 688
Hpy188III TCNNGA 2 cut(s) 71, 608
Hpy8I GTNNAC 7 cut(s) 6, 204, 212, 239, 568, 631, 639
Hpy99I CGWCG 4 cut(s) 80, 256, 523, 653
HpyAV CCTTC 1 cut(s) 494
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 377, 386, 499, 584, 670
HpyF3I CTNAG 2 cut(s) 429, 546
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 3 cut(s) 297, 464, 620
KflI GGGWCCC 1 cut(s) 322
KpnI GGTACC 1 cut(s) 360
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 3 cut(s) 232, 425, 505
LpnPI CCDG 7 cut(s) 22, 110, 135, 200, 355, 366, 516
Lsp1109I GCAGC 2 cut(s) 388, 589
MaeI CTAG 2 cut(s) 71, 144
MaeII ACGT 1 cut(s) 206
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 3 cut(s) 59, 539, 665
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 3 cut(s) 86, 150, 268
MlyI GAGTC 2 cut(s) 563, 626
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 679
MspI CCGG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 686
MvnI CGCG 2 cut(s) 308, 581
MwoI GCNNNNNNNGC 6 cut(s) 110, 377, 386, 499, 584, 670
NcoI CCATGG 1 cut(s) 293
NdeII GATC 1 cut(s) 177
NlaIII CATG 3 cut(s) 297, 464, 620
NlaIV GGNNCC 6 cut(s) 189, 323, 324, 340, 358, 421
PcsI WCGNNNNNNNCGW 1 cut(s) 578
PctI GAATGC 1 cut(s) 686
PfeI GAWTC 1 cut(s) 50
PflMI CCANNNNNTGG 1 cut(s) 348
PkrI GCNGC 5 cut(s) 291, 310, 403, 580, 604
PleI GAGTC 2 cut(s) 563, 626
PmaCI CACGTG 1 cut(s) 207
PmlI CACGTG 1 cut(s) 207
PpsI GAGTC 2 cut(s) 563, 626
Ppu21I YACGTR 1 cut(s) 207
PpuMI RGGWCCY 2 cut(s) 187, 322
Psp5II RGGWCCY 2 cut(s) 187, 322
PspCI CACGTG 1 cut(s) 207
PspN4I GGNNCC 6 cut(s) 189, 323, 324, 340, 358, 421
PspPI GGNCC 4 cut(s) 187, 322, 339, 475
PspPPI RGGWCCY 2 cut(s) 187, 322
PstNI CAGNNNCTG 1 cut(s) 14
RsaI GTAC 3 cut(s) 133, 305, 358
RsaNI GTAC 3 cut(s) 132, 304, 357
RseI CAYNNNNRTG 1 cut(s) 679
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 5 cut(s) 290, 309, 402, 579, 603
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 4 cut(s) 187, 322, 339, 475
ScaI AGTACT 1 cut(s) 133
SchI GAGTC 2 cut(s) 563, 626
SduI GDGCHC 1 cut(s) 43
SinI GGWCC 3 cut(s) 187, 322, 339
SmiMI CAYNNNNRTG 1 cut(s) 679
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
Sse9I AATT 3 cut(s) 86, 150, 268
SsiI CCGC 7 cut(s) 58, 230, 289, 308, 311, 371, 579
SspMI CTAG 2 cut(s) 71, 144
StyI CCWWGG 1 cut(s) 293
TaiI ACGT 1 cut(s) 209
TaqI TCGA 4 cut(s) 32, 161, 254, 572
TaqII GACCGA 1 cut(s) 411
TasI AATT 3 cut(s) 86, 150, 268
TatI WGTACW 1 cut(s) 131
TauI GCSGC 3 cut(s) 292, 311, 581
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TseI GCWGC 2 cut(s) 401, 602
TspDTI ATGAA 1 cut(s) 605
TspGWI ACGGA 1 cut(s) 507
Van91I CCANNNNNTGG 1 cut(s) 348
VpaK11BI GGWCC 3 cut(s) 187, 322, 339
XagI CCTNNNNNAGG 1 cut(s) 364
XapI RAATTY 1 cut(s) 86
XbaI TCTAGA 1 cut(s) 70
XspI CTAG 2 cut(s) 71, 144
ZrmI AGTACT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.