Rh1BG171500

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004245
Physical Location & Seq
Forward (+)
27380024 .. 27381357
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG171500.1

Sequence Viewer

Length: 720 bp
ATGTCCATAGAAACCGAAAGCTTCTCGAACTCAGACTGGGCTCTCTTGGAGTCGATCCGCCAGCATCTTCTTGACGACGATTTCGATACCCAGTTGGGTTTTTTCGCAGATTCTCCTGCTCAAAGTACTTCCAGCTCTAACAGCACTGAGATATCGAACAGTGATGCGGAGATAGTGGGGCAGAACCAAGCATTCACTAGTGAATTTGAGGAGGAGGTGGCGGAGCGTAACCACCACGCGCCGTCGGCACCGAGGCATTTTAGGGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAGAAGAACGGGATGAGAGTGTGGCTCGGAACCTACGAGACGGCGGAGGATGCCGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCCTCACTTGATTGGCACGCATGATATGGAGGTGGGCCGTGTGTTTCCCAAGCGTGCCTCGCCGGAGCCGTCATCTCCGTCCTCCACGACGTCGTCTGAAGATGGCTTGCCGAAGCCAAAGAGGAGGAGGGGCGGAGTGGACTCGGCTGCCCCGAAAGCCAAGTTGGAGGAAGCTGCTCAAGAGTTTCATGTGTTCCAAGTGGACTCATTCACATTTTGCAACGAACTATTGGTTGGGGATATGGCGCTCTTGTCACCATCTGAATGCTCAGACATTCTTTCTTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

239

Amino Acids

26.5

Weight (kDa)

5.22

Isoelectric Point (pI)

64.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 86 - 137 8.2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 247
AccII CGCG 2 cut(s) 239, 296
AciI CCGC 9 cut(s) 58, 167, 221, 277, 296, 299, 359, 390, 561
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 278
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 127, 293
AgsI TTSAA 1 cut(s) 397
AhlI ACTAGT 1 cut(s) 197
AjuI GAANNNNNNNTTGG 4 cut(s) 575, 607, 645, 677
AluBI AGCT 5 cut(s) 21, 135, 416, 421, 602
AluI AGCT 5 cut(s) 21, 135, 416, 421, 602
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 49
AoxI GGCC 2 cut(s) 278, 463
ApeKI GCWGC 2 cut(s) 575, 602
ApoI RAATTY 1 cut(s) 203
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 2 cut(s) 241, 676
AspS9I GGNCC 2 cut(s) 310, 463
AsuHPI GGTGA 1 cut(s) 675
AvaII GGWCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 562, 589
BccI CCATC 2 cut(s) 524, 694
BceAI ACGGC 5 cut(s) 226, 265, 372, 450, 481
BcoDI GTCTC 1 cut(s) 347
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 677
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 5 cut(s) 278, 297, 390, 576, 603
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 5 cut(s) 279, 298, 391, 577, 604
BmcAI AGTACT 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 310, 463
BmiI GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
BmrI ACTGGG 2 cut(s) 46, 85
BmsI GCATC 3 cut(s) 73, 154, 355
BmuI ACTGGG 2 cut(s) 46, 85
BplI GAGNNNNNCTC 2 cut(s) 324, 356
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 251, 281
Bse1I ACTGG 2 cut(s) 41, 91
BseDI CCNNGG 2 cut(s) 251, 281
BseGI GGATG 2 cut(s) 333, 370
BseMII CTCAG 3 cut(s) 45, 138, 711
BseNI ACTGG 2 cut(s) 41, 91
BseRI GAGGAG 5 cut(s) 224, 227, 286, 565, 568
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 562, 589
Bsh1236I CGCG 2 cut(s) 239, 296
Bsh1285I CGRYCG 2 cut(s) 281, 385
BshFI GGCC 2 cut(s) 280, 465
BshNI GGYRCC 1 cut(s) 247
BsiEI CGRYCG 2 cut(s) 281, 385
BsiSI CCGG 2 cut(s) 369, 491
BslFI GGGAC 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 2 cut(s) 191, 698
BsnI GGCC 2 cut(s) 280, 465
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 9 cut(s) 58, 167, 221, 277, 296, 299, 359, 390, 561
BspANI GGCC 2 cut(s) 280, 465
BspCNI CTCAG 3 cut(s) 44, 139, 710
BspFNI CGCG 2 cut(s) 239, 296
BspLI GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 247
BsrI ACTGG 2 cut(s) 41, 91
BssECI CCNNGG 2 cut(s) 251, 281
BssMI GATC 1 cut(s) 54
BssNI GRCGYC 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 161
BstACI GRCGYC 1 cut(s) 518
BstC8I GCNNGC 4 cut(s) 62, 446, 483, 536
BstDEI CTNAG 4 cut(s) 31, 147, 417, 697
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 333, 370
BstFNI CGCG 2 cut(s) 239, 296
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 2 cut(s) 241, 676
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 1 cut(s) 54
BstMCI CGRYCG 2 cut(s) 281, 385
BstMWI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 584
BstUI CGCG 2 cut(s) 239, 296
BstV1I GCAGC 2 cut(s) 562, 589
BstZI CGGCCG 1 cut(s) 278
BsuRI GGCC 2 cut(s) 280, 465
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 2 cut(s) 333, 370
BtsIMutI CAGTG 2 cut(s) 144, 166
Cac8I GCNNGC 4 cut(s) 62, 446, 483, 536
CfoI GCGC 2 cut(s) 241, 676
Cfr13I GGNCC 2 cut(s) 310, 463
Csp6I GTAC 2 cut(s) 126, 292
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 2 cut(s) 449, 617
CviQI GTAC 2 cut(s) 126, 292
DdeI CTNAG 4 cut(s) 31, 147, 417, 697
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
EaeI YGGCCR 1 cut(s) 278
EagI CGGCCG 1 cut(s) 278
EciI GGCGGA 5 cut(s) 47, 236, 314, 374, 576
EclXI CGGCCG 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 310
EcoRV GATATC 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 2 cut(s) 452, 620
FaiI YATR 5 cut(s) 8, 450, 455, 618, 671
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 410, 442
FaqI GGGAC 1 cut(s) 323
FatI CATG 2 cut(s) 448, 616
Fnu4HI GCNGC 5 cut(s) 278, 297, 390, 576, 603
FokI GGATG 2 cut(s) 340, 377
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 5 cut(s) 278, 297, 390, 576, 603
FspBI CTAG 1 cut(s) 198
GlaI GCGC 2 cut(s) 240, 675
GluI GCNGC 5 cut(s) 278, 297, 390, 576, 603
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 2 cut(s) 280, 465
HapII CCGG 2 cut(s) 369, 491
HhaI GCGC 2 cut(s) 241, 676
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 2 cut(s) 452, 620
Hin6I GCGC 2 cut(s) 239, 674
HinP1I GCGC 2 cut(s) 239, 674
HindIII AAGCTT 2 cut(s) 19, 419
HinfI GANTC 4 cut(s) 50, 110, 569, 632
HpaII CCGG 2 cut(s) 369, 491
HphI GGTGA 1 cut(s) 675
Hpy166II GTNNAC 2 cut(s) 568, 631
Hpy188I TCNGA 5 cut(s) 34, 344, 526, 691, 700
Hpy188III TCNNGA 3 cut(s) 25, 71, 608
Hpy8I GTNNAC 2 cut(s) 568, 631
Hpy99I CGWCG 4 cut(s) 80, 247, 520, 523
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 1 cut(s) 648
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 584
HpyF3I CTNAG 4 cut(s) 31, 147, 417, 697
HpySE526I ACGT 1 cut(s) 518
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 2 cut(s) 452, 620
HspAI GCGC 2 cut(s) 239, 674
KflI GGGWCCC 1 cut(s) 310
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 3 cut(s) 223, 413, 493
LpnPI CCDG 7 cut(s) 22, 74, 104, 129, 145, 382, 504
Lsp1109I GCAGC 2 cut(s) 562, 589
LweI GCATC 3 cut(s) 73, 154, 355
MaeI CTAG 1 cut(s) 198
MaeII ACGT 1 cut(s) 518
MaeIII GTNAC 2 cut(s) 227, 681
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 4 cut(s) 59, 328, 331, 539
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 1 cut(s) 203
MlyI GAGTC 3 cut(s) 59, 563, 626
MmeI TCCRAC 1 cut(s) 573
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 691
MspI CCGG 2 cut(s) 369, 491
Mva1269I GAATGC 2 cut(s) 191, 698
MvnI CGCG 2 cut(s) 239, 296
MwoI GCNNNNNNNGC 6 cut(s) 141, 245, 365, 374, 487, 584
NdeII GATC 1 cut(s) 54
NlaIII CATG 2 cut(s) 452, 620
NlaIV GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
NmeAIII GCCGAG 1 cut(s) 551
NmuCI GTSAC 1 cut(s) 681
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 348, 494, 578
PctI GAATGC 2 cut(s) 191, 698
PfeI GAWTC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 520
PkrI GCNGC 5 cut(s) 279, 298, 391, 577, 604
PleI GAGTC 3 cut(s) 58, 563, 626
PpsI GAGTC 3 cut(s) 58, 563, 626
PpuMI RGGWCCY 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 310
PspN4I GGNNCC 6 cut(s) 249, 311, 312, 346, 409, 495
PspPI GGNCC 2 cut(s) 310, 463
PspPPI RGGWCCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 520
RsaI GTAC 2 cut(s) 127, 293
RsaNI GTAC 2 cut(s) 126, 292
RseI CAYNNNNRTG 1 cut(s) 691
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 5 cut(s) 278, 297, 390, 576, 603
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 310, 463
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 3 cut(s) 59, 563, 626
SduI GDGCHC 1 cut(s) 43
SetI ASST 9 cut(s) 23, 137, 219, 350, 418, 423, 462, 521, 604
SfaNI GCATC 3 cut(s) 73, 154, 355
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 691
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 1 cut(s) 203
SsiI CCGC 9 cut(s) 58, 167, 221, 277, 296, 299, 359, 390, 561
SspMI CTAG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 1 cut(s) 521
TaqI TCGA 4 cut(s) 26, 53, 84, 155
TaqII GACCGA 1 cut(s) 399
TasI AATT 1 cut(s) 203
TatI WGTACW 1 cut(s) 125
TauI GCSGC 3 cut(s) 280, 299, 392
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 2 cut(s) 151, 166
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 2 cut(s) 575, 602
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 1 cut(s) 605
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 2 cut(s) 151, 166
Tth111I GACNNNGTC 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 203
XspI CTAG 1 cut(s) 198
ZraI GACGTC 1 cut(s) 519
ZrmI AGTACT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.