Rw1G017260

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004245
Physical Location & Seq
Forward (+)
38249458 .. 38250170
713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017260.1

Sequence Viewer

Length: 657 bp
ATGTCCACAGAAACAAGCTTCTCAAACTCAGACTGGGCTCTCTTGGAATCGATCCGCCAGCATCTTCTCGAAGACGATTTCGATACCAGTCGGCATTTTCCTCTGACTCTCCAGGCTCCAGCTCCTGGAACTGCACTGAGATATCGAACAGTGGCGGCGCGTGAACACCACACACCATCGGGTCAGAGGCATTTTAGAGGAGTGAGGAGGCGGCCGTGGGGAAAGTACGCGGCGGAGATAAGGGACCCGAAAAAGAACGGGACGAGAGTCTGGCTCGGGACCTACGAGAAGGCGGAGGACGCAGGATTGGCTTACGACCGAGCCGCTTTCAAAATGCGTGGCTCCAAAGCTAAGCTTAACTTTCTTCACTTGATTGGCATGCATGATGTGGAGGTGGGCCGTGTGCTTCCCAAGCGTGCCTCGCCGGAGCCGTCATCTCCGTCCTCCACGACGTCGTCTAAAGATGGCTCGCCGAAGCCAAAGAGGAGGAGGGGTGGAGTGGGCTCGGCTGCGGCGGAAGCCCAGTTGGAGGAAGCTGCTCAAGAGTTTCATGTGTTCCAAGTGGACTCATTCACATTTTGCAACGAACTATTGGTTGAGGATATGGCGCTCTTGTCACCATCTGAATGCTCAGACATTCTTTCTTTTTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

218

Amino Acids

24.32

Weight (kDa)

7.85

Isoelectric Point (pI)

60.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 64 - 115 8.7e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 455
AccB7I CCANNNNNTGG 1 cut(s) 125
AccII CGCG 2 cut(s) 160, 230
AciI CCGC 9 cut(s) 55, 155, 211, 230, 233, 293, 324, 512, 515
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 452
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 125, 529
AgsI TTSAA 1 cut(s) 331
AjnI CCWGG 2 cut(s) 111, 124
AluBI AGCT 5 cut(s) 18, 122, 350, 355, 536
AluI AGCT 5 cut(s) 18, 122, 350, 355, 536
AlwI GGATC 1 cut(s) 46
AlwNI CAGNNNCTG 1 cut(s) 125
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 2 cut(s) 212, 397
ApeKI GCWGC 2 cut(s) 509, 536
AspLEI GCGC 2 cut(s) 160, 610
AspS9I GGNCC 3 cut(s) 244, 279, 397
AsuHPI GGTGA 1 cut(s) 609
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 2 cut(s) 244, 279
BanII GRGCYC 2 cut(s) 40, 506
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 496, 523
BccI CCATC 3 cut(s) 184, 458, 628
BceAI ACGGC 3 cut(s) 199, 384, 415
BciT130I CCWGG 2 cut(s) 113, 126
BfoI RGCGCY 1 cut(s) 611
BisI GCNGC 7 cut(s) 156, 212, 231, 324, 510, 513, 537
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 7 cut(s) 157, 213, 232, 325, 511, 514, 538
Bme1390I CCNGG 2 cut(s) 113, 126
Bme18I GGWCC 2 cut(s) 244, 279
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 3 cut(s) 244, 279, 397
BmiI GGNNCC 6 cut(s) 117, 245, 246, 280, 343, 429
BmrFI CCNGG 2 cut(s) 113, 126
BmrI ACTGGG 2 cut(s) 43, 517
BmsI GCATC 1 cut(s) 70
BmuI ACTGGG 2 cut(s) 43, 517
BoxI GACNNNNGTC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 2 cut(s) 95, 102
Bpu1102I GCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 525
Bsa29I ATCGAT 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 452
BsaJI CCNNGG 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 529
Bse1I ACTGG 3 cut(s) 38, 87, 523
BseBI CCWGG 2 cut(s) 113, 126
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 215
BseLI CCNNNNNNNGG 2 cut(s) 125, 529
BseMII CTCAG 3 cut(s) 42, 128, 645
BseNI ACTGG 3 cut(s) 38, 87, 523
BseRI GAGGAG 4 cut(s) 213, 220, 499, 502
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 496, 523
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 160, 230
Bsh1285I CGRYCG 2 cut(s) 215, 319
BshFI GGCC 2 cut(s) 214, 399
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 2 cut(s) 215, 319
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 3 cut(s) 257, 274, 292
BslI CCNNNNNNNGG 2 cut(s) 125, 529
BsmFI GGGAC 3 cut(s) 257, 274, 292
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 2 cut(s) 214, 399
BsoBI CYCGRG 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 40, 506
Bsp143I GATC 1 cut(s) 51
Bsp1720I GCTNAGC 1 cut(s) 351
BspACI CCGC 9 cut(s) 55, 155, 211, 230, 233, 293, 324, 512, 515
BspANI GGCC 2 cut(s) 214, 399
BspCNI CTCAG 3 cut(s) 41, 129, 644
BspDI ATCGAT 1 cut(s) 50
BspFNI CGCG 2 cut(s) 160, 230
BspLI GGNNCC 6 cut(s) 117, 245, 246, 280, 343, 429
BspPI GGATC 1 cut(s) 46
BsrI ACTGG 3 cut(s) 38, 87, 523
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 452
Bst2UI CCWGG 2 cut(s) 113, 126
Bst4CI ACNGT 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 452
BstC8I GCNNGC 4 cut(s) 59, 380, 417, 470
BstDEI CTNAG 4 cut(s) 28, 137, 351, 631
BstDSI CCRYGG 1 cut(s) 215
BstFNI CGCG 2 cut(s) 160, 230
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 2 cut(s) 160, 610
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 2 cut(s) 215, 319
BstMWI GCNNNNNNNGC 5 cut(s) 299, 308, 412, 421, 518
BstNI CCWGG 2 cut(s) 113, 126
BstNSI RCATGY 1 cut(s) 382
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 111, 124
BstUI CGCG 2 cut(s) 160, 230
BstV1I GCAGC 2 cut(s) 496, 523
BstV2I GAAGAC 1 cut(s) 78
BstZI CGGCCG 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 2 cut(s) 214, 399
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 134, 156
Cac8I GCNNGC 4 cut(s) 59, 380, 417, 470
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 2 cut(s) 160, 610
Cfr13I GGNCC 3 cut(s) 244, 279, 397
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 226
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 3 cut(s) 379, 383, 551
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 4 cut(s) 28, 137, 351, 631
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 212
EagI CGGCCG 1 cut(s) 212
EciI GGCGGA 4 cut(s) 44, 248, 308, 530
EclXI CGGCCG 1 cut(s) 212
Eco24I GRGCYC 2 cut(s) 40, 506
Eco32I GATATC 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 244, 279
Eco52I CGGCCG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 275
EcoO109I RGGNCCY 2 cut(s) 244, 279
EcoRII CCWGG 2 cut(s) 111, 124
EcoRV GATATC 1 cut(s) 143
EcoT22I ATGCAT 1 cut(s) 384
EcoT38I GRGCYC 2 cut(s) 40, 506
FaeI CATG 3 cut(s) 382, 386, 554
FaiI YATR 4 cut(s) 380, 384, 552, 605
FalI AAGNNNNNCTT 4 cut(s) 339, 371, 344, 376
FaqI GGGAC 3 cut(s) 257, 274, 292
FatI CATG 3 cut(s) 378, 382, 550
Fnu4HI GCNGC 7 cut(s) 156, 212, 231, 324, 510, 513, 537
FriOI GRGCYC 2 cut(s) 40, 506
Fsp4HI GCNGC 7 cut(s) 156, 212, 231, 324, 510, 513, 537
GlaI GCGC 2 cut(s) 159, 609
GluI GCNGC 7 cut(s) 156, 212, 231, 324, 510, 513, 537
GsuI CTGGAG 2 cut(s) 95, 102
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 2 cut(s) 214, 399
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 308
HhaI GCGC 2 cut(s) 160, 610
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 3 cut(s) 382, 386, 554
Hin6I GCGC 2 cut(s) 158, 608
HinP1I GCGC 2 cut(s) 158, 608
HindIII AAGCTT 2 cut(s) 16, 353
HinfI GANTC 4 cut(s) 47, 106, 267, 566
HpaII CCGG 1 cut(s) 425
HphI GGTGA 1 cut(s) 609
Hpy166II GTNNAC 3 cut(s) 6, 164, 565
Hpy188I TCNGA 5 cut(s) 31, 105, 186, 625, 634
Hpy188III TCNNGA 3 cut(s) 68, 277, 542
Hpy8I GTNNAC 3 cut(s) 6, 164, 565
Hpy99I CGWCG 2 cut(s) 454, 457
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 3 cut(s) 134, 382, 582
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 308, 412, 421, 518
HpyF3I CTNAG 4 cut(s) 28, 137, 351, 631
HpySE526I ACGT 1 cut(s) 452
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 3 cut(s) 382, 386, 554
HspAI GCGC 2 cut(s) 158, 608
KflI GGGWCCC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 4 cut(s) 121, 127, 347, 427
Lsp1109I GCAGC 2 cut(s) 496, 523
LweI GCATC 1 cut(s) 70
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 1 cut(s) 615
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 3 cut(s) 56, 83, 356
MhlI GDGCHC 2 cut(s) 40, 506
MlyI GAGTC 3 cut(s) 100, 276, 560
MmeI TCCRAC 1 cut(s) 507
Mph1103I ATGCAT 1 cut(s) 384
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 625
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 113, 126
Mva1269I GAATGC 1 cut(s) 632
MvaI CCWGG 2 cut(s) 113, 126
MvnI CGCG 2 cut(s) 160, 230
MwoI GCNNNNNNNGC 5 cut(s) 299, 308, 412, 421, 518
NdeII GATC 1 cut(s) 51
NlaIII CATG 3 cut(s) 382, 386, 554
NlaIV GGNNCC 6 cut(s) 117, 245, 246, 280, 343, 429
NmeAIII GCCGAG 1 cut(s) 485
NmuCI GTSAC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 384
NspI RCATGY 1 cut(s) 382
PaeI GCATGC 1 cut(s) 382
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 428
PctI GAATGC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 47
PflFI GACNNNGTC 1 cut(s) 454
PflMI CCANNNNNTGG 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 7 cut(s) 157, 213, 232, 325, 511, 514, 538
PleI GAGTC 3 cut(s) 100, 275, 560
PpsI GAGTC 3 cut(s) 100, 275, 560
PpuMI RGGWCCY 2 cut(s) 244, 279
PshAI GACNNNNGTC 1 cut(s) 266
Psp5II RGGWCCY 2 cut(s) 244, 279
Psp6I CCWGG 2 cut(s) 111, 124
PspGI CCWGG 2 cut(s) 111, 124
PspN4I GGNNCC 6 cut(s) 117, 245, 246, 280, 343, 429
PspPI GGNCC 3 cut(s) 244, 279, 397
PspPPI RGGWCCY 2 cut(s) 244, 279
PstNI CAGNNNCTG 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 454
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 625
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 7 cut(s) 156, 212, 231, 324, 510, 513, 537
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 3 cut(s) 244, 279, 397
SchI GAGTC 3 cut(s) 100, 276, 560
ScrFI CCNGG 2 cut(s) 113, 126
SduI GDGCHC 2 cut(s) 40, 506
SetI ASST 8 cut(s) 20, 124, 284, 352, 357, 396, 455, 538
SfaNI GCATC 1 cut(s) 70
SinI GGWCC 2 cut(s) 244, 279
SmiMI CAYNNNNRTG 1 cut(s) 625
SmlI CTYRAG 1 cut(s) 540
SmoI CTYRAG 1 cut(s) 540
SphI GCATGC 1 cut(s) 382
SsiI CCGC 9 cut(s) 55, 155, 211, 230, 233, 293, 324, 512, 515
StyD4I CCNGG 2 cut(s) 111, 124
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 1 cut(s) 455
TaqI TCGA 4 cut(s) 50, 69, 81, 145
TaqII GACCGA 1 cut(s) 333
TauI GCSGC 5 cut(s) 158, 214, 233, 326, 515
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 2 cut(s) 141, 156
TseFI GTSAC 1 cut(s) 615
TseI GCWGC 2 cut(s) 509, 536
Tsp45I GTSAC 1 cut(s) 615
TspDTI ATGAA 1 cut(s) 539
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 2 cut(s) 141, 156
Tth111I GACNNNGTC 1 cut(s) 454
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 244, 279
XceI RCATGY 1 cut(s) 382
ZraI GACGTC 1 cut(s) 453
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.