Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002317

Physical Location & Seq

Genomic Coordinates Reverse (-)

11176998 .. 11185927

Gene Structure 8930 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1060100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGGCAGACAGTGAGACAGTTGTTGCTCAAACATCTGCAGTGATGGGGTATACATCAGCAGGATATACTTCAACAGGATATGCTGATGCCAGCTCAAATATTGCTACTGATACGGGTGCATTGCATTCTGAGGCTACCCCAGAGTTTTCTAATACACCTGGCGGTTCTAACATAGGTGATGGAAATGCATATTCTGCGGATCCCAATTCTCTCATGCAAGAAACACAAAGTTACACAGCATATGAGACCAAACCAGCTGTTGGAGTGACAGATGCTAGTGGGAATGTTGTTAGTAATGAAAATGACGCCTTGGAGTCATCTCAAGTTGCTAGTTATGATTCTTCTATGAATGGTGTTATTGCTTCTGGAGTGGGAACTGTTTCATCAGTTGATAATGGAAATGCCGAACCTCTTGATCCACAACAATTTGGGGATGCTTCTGCCATGTCTGTCGAAGAAGATAGACTGTGGAACATTGTGAGGGCTAATTCCTTGGATTTCAATGCCTGGACTGCTTTAATTGAAGAGACAGAGAAGATTGCACAGGATAACATATTGAAAATTCGGAAAGTTTATGATGCTTTTTTAGCAGAGTTTCCGTTATGTTATGGTTATTGGAAAAAGTATGCGGATCATGAGGCACGTCTAGGTTCTATCGATAAAGTTGTGGAGGTTTATGAACGTGCAGTTCAAGGTGTTACCTATTCAGTTGATGTCTGGTTGCATTACTGCATTTTTGCTATAAGCACATATGGAGATCCAGACACTGTTAGAAGGCTTTTTGAGCGAGGACTTGCTTATGTTGGAACAGATTACCTGTCCTTTACTCTGTGGGATAAGTATATAGAATATGAGTACATGCAGCAGGAGTGGGGCCGCCTTGCCATGATCTATTCACGAATATTAGAGAACCCAAATCAGCAACTGGATCGGTATTGGAACAGCTTTAAGGAGTTAGCTGGAAGTCGGCCACTCTCAGAATTGAGGACTGCTGAAGAAGCTGCAGCAGCAGGAGGTGCACATTCAGAGGGTATTGACAAAGTAAATGGGGAAGATGTTCAATCTGATGGTGCAGAACAATCTCCTAAACCTGTAAGTGCTGACTTAACAGAAGCCGAGGAGTTGGAGAAGTTTATCACCATTAGAGAAGAGGTGTATAAGAAAGCTAAAGAGTTCGATTCTAAGATCATTAGTTTTGAAACAGCTATTAGGAGGCCCTACTTTCACGTGCGGCCTCTCAATTCTGTAGAGCTTGAAAATTGGACCAGCTACCTAGACTTTATAGAAAGGGAGGGCGACTTGAGCAAGGTTGTCAAGCTATATGAAAGGTGCCTAATTGCTTGTGCTAATTATCCTGAATACTGGATTCGATATGTTCTATGCATGGAGGCCAGTGGAAGTATGGATATTGCCAATAATGCTCTTGCACGTGCAACCCAGGTTTTTGTGAAGAGACAACCGGAGATGCACCTTTTTGCTGCTCGATTCAAAGAGCAGAGTGGGGATATACCTGGTGCTCAAGCTGCCTATCAACTTGTACATTCTGAACTCTCGCCTGGTCTTCTTGAAGCAATCGTGAAGCATGCAAATATGGAACACCGTTTGGGAAATGTGGAAGCTGCCTACTCTTTATACGAACAGGCCATTGCCATTGAAAAAGTAAAAGAGCATTCACAGACGTTACCCATGTTGTATGCTCAGTACTCTCGGTTCACTTACCTGGTTTCTTTGAATGCGGAAAAAGCTAGAGAAATTTTAGATGGAGCACTCAAGCATGTACAACTGTCAAGAGCATTTCTTGAGTCCATGATGGTTTTCGAGACAATACAGCCACTGCCAAAGCGAATTGATCACATAGATTCATTGGTTGTGAAGTTCATAGAGCCCAGCTCAGAGAGCCCCAATTTTGCTAGCGTTGCTGACAGAGAAGAGCTATCTGGCATCTATTTGGAGTTTGTTAATCTCTTTGGAGATGCACAGTCTATCAAGAAGGCTGAGGATCGACATGCTAAGCTCTTCATACCTCATAGGAGCAGCACGTCAGAGTTGAAAAAACGTCAAGCTGAGGACTTTCTATCTTCAGATAAAGTGAAGCTAGCTAAAGCTTACTCTGGTGTTCCATCGCCCGCCCAGTCGATCATGGGAGCATATCCAAATGCACAGAGCCAATGGCCAGCTGGTTATGGTGCACAGCCTCAAGCTTGGCCACCAGCTACGCAAGCTACTCAGGGACAACAGTGGCCAGCTGGTTATACCCAACAGCCGTACAATGCATATGGTGGTTATGGAACTGCTGGCTACGCTAATCCGCAAGCGCCCGTGGTGGCGCCACAAACTGCTGCTTACGGTGCTTATCCTTCTACGTATCCTGCTCAGCAGGCCGTCCCGCCACAAAGTTACGCGCATCCAACCGTGGCAGCAGTAGCACCGGCTCCGCAGCCTGCTTCAGTTCCTCAGGCGTATTATGGGAGTTACTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000243 GO:0000245 GO:0000375 GO:0000377 GO:0000395 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005685 GO:0006139 GO:0006376 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0065003 GO:0071004 GO:0071010 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0120114 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

827

Amino Acids

91.29

Weight (kDa)

4.85

Isoelectric Point (pI)

43.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_PRP39_N	PF23240	154 - 318	2.6e-61	PRP39 N-terminal HAT repeat
Suf	PF05843	156 - 318	4e-15	Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_N	PF23233	203 - 322	5.7e-08	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
Suf	PF05843	376 - 592	6.3e-07	Suppressor of forked protein (Suf)
HAT_PRP39_C	PF23241	395 - 534	1.2e-46	PRP39 C-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04080 AT1G04080 AT1G04080 AT1G04080 AT1G04080
fragaria_vesca	FvH4_2g20550
malus_domestica	MD00G1060100.v1.1
prunus_persica	Prupe.8G215900_v2.0.a1 Prupe.8G215900_v2.0.a1 Prupe.8G215900_v2.0.a1 Prupe.8G215900_v2.0.a1
pyrus_communis	pycom05g17390 pycom10g15270
rosa_chinensis	RchiOBHm_Chr1g0327141 RchiOBHm_Chr6g0282561 RchiOBHm_Chr6g0286711
rosa_laevigata	RLG00000007391 RLG00000012550 RLG00000030995
rosa_multiflora	Rmu_co8283097.1_g000001 Rmu_co8332197.1_g000001 Rmu_sc0001468.1_g000022
rosa_roxburghii	Rroxscaffold_1G00034270 Rroxscaffold_1G00060390 Rroxscaffold_7G00182090
rosa_rugosa	Rorug05G0468800 Rorug06G0182100 Rorug06G0182200
rosa_samantha	Rh2CG306300 Rh5BG355000 Rh5BG355100 Rh5BG357900 Rh6AG023000 Rh6AG294900 Rh6BG204700 Rh6BG298100 Rh6BG298200 Rh6CG298600 Rh6DG290600
rosa_wichuraiana	Rw1G013170 Rw5G035150 Rw6G025370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1329, 2326
AccB7I	CCANNNNNTGG	1 cut(s)	260
AccI	GTMKAC	1 cut(s)	50
AccII	CGCG	1 cut(s)	2402
AclWI	GGATC	7 cut(s)	194, 207, 410, 639, 752, 936, 2007
AcoI	YGGCCR	4 cut(s)	968, 2171, 2204, 2240
AcsI	RAATTY	2 cut(s)	561, 1752
AcuI	CTGAAG	3 cut(s)	1014, 2064, 2430
AcvI	CACGTG	2 cut(s)	1228, 1430
AcyI	GRCGYC	2 cut(s)	306, 2327
AfaI	GTAC	5 cut(s)	857, 1539, 1703, 1779, 2267
AfiI	CCNNNNNNNGG	1 cut(s)	260
AjiI	CACGTC	2 cut(s)	644, 2040
AjnI	CCWGG	6 cut(s)	157, 506, 1437, 1510, 1555, 1719
Alw21I	GWGCWC	4 cut(s)	1021, 1519, 1768, 2191
Alw26I	GTCTC	5 cut(s)	8, 239, 521, 1447, 1814
Alw44I	GTGCAC	2 cut(s)	1017, 2187
AlwI	GGATC	7 cut(s)	194, 207, 410, 639, 752, 936, 2007
AlwNI	CAGNNNCTG	3 cut(s)	767, 925, 1834
ApaLI	GTGCAC	2 cut(s)	1017, 2187
ApoI	RAATTY	2 cut(s)	561, 1752
Asp700I	GAANNNNTTC	2 cut(s)	379, 1056
AspLEI	GCGC	3 cut(s)	2317, 2329, 2404
AspS9I	GGNCC	3 cut(s)	874, 1215, 1263
AsuHPI	GGTGA	2 cut(s)	188, 1129
AsuNHI	GCTAGC	2 cut(s)	1910, 2095
AvaII	GGWCC	1 cut(s)	1263
AxyI	CCTNAGG	1 cut(s)	2454
BaeGI	GKGCMC	2 cut(s)	1021, 2191
BalI	TGGCCA	3 cut(s)	2173, 2206, 2242
BamHI	GGATCC	1 cut(s)	199
BanI	GGYRCC	2 cut(s)	1329, 2326
BanII	GRGCYC	2 cut(s)	1887, 1901
BbrPI	CACGTG	2 cut(s)	1228, 1430
BbsI	GAAGAC	1 cut(s)	1553
Bbv12I	GWGCWC	4 cut(s)	1021, 1519, 1768, 2191
BbvCI	CCTCAGC	2 cut(s)	1995, 2064
BccI	CCATC	6 cut(s)	37, 173, 1061, 1754, 1804, 2128
BceAI	ACGGC	2 cut(s)	2248, 2366
BcgI	CGANNNNNNTGC	2 cut(s)	911, 945
BciT130I	CCWGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
BciVI	GTATCC	1 cut(s)	2376
BclI	TGATCA	1 cut(s)	1849
BcoDI	GTCTC	5 cut(s)	8, 239, 521, 1447, 1814
BfaI	CTAG	7 cut(s)	276, 330, 647, 1274, 1746, 1911, 2096
BfmI	CTRYAG	3 cut(s)	36, 1002, 1245
BfoI	RGCGCY	2 cut(s)	2318, 2330
BfuI	GTATCC	1 cut(s)	2376
BlpI	GCTNAGC	2 cut(s)	2010, 2373
BmcAI	AGTACT	1 cut(s)	1703
Bme1390I	CCNGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
Bme18I	GGWCC	1 cut(s)	1263
BmgBI	CACGTC	2 cut(s)	644, 2040
BmgT120I	GGNCC	3 cut(s)	874, 1215, 1263
BmiI	GGNNCC	5 cut(s)	201, 875, 1331, 2328, 2433
BmrFI	CCNGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
BmrI	ACTGGG	1 cut(s)	2125
BmsI	GCATC	8 cut(s)	76, 262, 424, 568, 1455, 1950, 1963, 2413
BmtI	GCTAGC	2 cut(s)	1914, 2099
BmuI	ACTGGG	1 cut(s)	2125
BpiI	GAAGAC	1 cut(s)	1553
BplI	GAGNNNNNCTC	2 cut(s)	1874, 1906
BpmI	CTGGAG	1 cut(s)	387
Bpu10I	CCTNAGC	2 cut(s)	1995, 2064
Bpu1102I	GCTNAGC	2 cut(s)	2010, 2373
BpuEI	CTTGAG	6 cut(s)	306, 1321, 1503, 1754, 1820, 2181
Bsa29I	ATCGAT	1 cut(s)	657
BsaAI	YACGTR	3 cut(s)	1228, 1430, 2364
BsaHI	GRCGYC	2 cut(s)	306, 2327
BsaI	GGTCTC	1 cut(s)	239
BsaJI	CCNNGG	6 cut(s)	309, 492, 1116, 1437, 2319, 2412
BsaWI	WCCGGW	1 cut(s)	1459
Bsc4I	CCNNNNNNNGG	1 cut(s)	260
Bse118I	RCCGGY	1 cut(s)	2428
Bse1I	ACTGG	4 cut(s)	930, 1367, 1392, 2131
Bse21I	CCTNAGG	1 cut(s)	2454
Bse3DI	GCAATG	2 cut(s)	119, 1644
BseBI	CCWGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
BseCI	ATCGAT	1 cut(s)	657
BseDI	CCNNGG	6 cut(s)	309, 492, 1116, 1437, 2319, 2412
BseGI	GGATG	2 cut(s)	439, 2404
BseLI	CCNNNNNNNGG	1 cut(s)	260
BseMI	GCAATG	2 cut(s)	119, 1644
BseMII	CTCAG	9 cut(s)	120, 990, 1712, 1905, 1986, 2055, 2240, 2387, 2468
BseNI	ACTGG	4 cut(s)	930, 1367, 1392, 2131
BseRI	GAGGAG	1 cut(s)	1133
BseSI	GKGCMC	2 cut(s)	1021, 2191
BseYI	CCCAGC	1 cut(s)	1886
BsgI	GTGCAG	2 cut(s)	705, 1092
Bsh1236I	CGCG	1 cut(s)	2402
BshNI	GGYRCC	2 cut(s)	1329, 2326
BshVI	ATCGAT	1 cut(s)	657
BsiHKAI	GWGCWC	4 cut(s)	1021, 1519, 1768, 2191
BsiSI	CCGG	2 cut(s)	1460, 2429
BslFI	GGGAC	2 cut(s)	2244, 2369
BslI	CCNNNNNNNGG	1 cut(s)	260
BsmAI	GTCTC	5 cut(s)	8, 239, 521, 1447, 1814
BsmFI	GGGAC	2 cut(s)	2244, 2369
BsmI	GAATGC	3 cut(s)	124, 1669, 1738
Bso31I	GGTCTC	1 cut(s)	239
Bsp1286I	GDGCHC	6 cut(s)	1021, 1519, 1768, 1887, 1901, 2191
Bsp1407I	TGTACA	2 cut(s)	1537, 1777
Bsp1720I	GCTNAGC	2 cut(s)	2010, 2373
BspCNI	CTCAG	9 cut(s)	121, 989, 1711, 1904, 1987, 2056, 2239, 2386, 2467
BspDI	ATCGAT	1 cut(s)	657
BspFNI	CGCG	1 cut(s)	2402
BspHI	TCATGA	1 cut(s)	634
BspLI	GGNNCC	5 cut(s)	201, 875, 1331, 2328, 2433
BspMAI	CTGCAG	2 cut(s)	40, 1006
BspOI	GCTAGC	2 cut(s)	1914, 2099
BspPI	GGATC	7 cut(s)	194, 207, 410, 639, 752, 936, 2007
BspQI	GCTCTTC	2 cut(s)	1923, 2021
BspT107I	GGYRCC	2 cut(s)	1329, 2326
BspTNI	GGTCTC	1 cut(s)	239
BsrDI	GCAATG	2 cut(s)	119, 1644
BsrFI	RCCGGY	1 cut(s)	2428
BsrGI	TGTACA	2 cut(s)	1537, 1777
BsrI	ACTGG	4 cut(s)	930, 1367, 1392, 2131
BssAI	RCCGGY	1 cut(s)	2428
BssECI	CCNNGG	6 cut(s)	309, 492, 1116, 1437, 2319, 2412
BssNAI	GTATAC	1 cut(s)	51
BssNI	GRCGYC	2 cut(s)	306, 2327
BssT1I	CCWWGG	2 cut(s)	309, 492
Bst1107I	GTATAC	1 cut(s)	51
Bst2UI	CCWGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
Bst6I	CTCTTC	5 cut(s)	519, 1143, 1445, 1923, 2021
BstACI	GRCGYC	2 cut(s)	306, 2327
BstAPI	GCANNNNNTGC	2 cut(s)	194, 1016
BstAUI	TGTACA	2 cut(s)	1537, 1777
BstBAI	YACGTR	3 cut(s)	1228, 1430, 2364
BstDSI	CCRYGG	2 cut(s)	2319, 2412
BstF5I	GGATG	2 cut(s)	439, 2404
BstFNI	CGCG	1 cut(s)	2402
BstH2I	RGCGCY	2 cut(s)	2318, 2330
BstHHI	GCGC	3 cut(s)	2317, 2329, 2404
BstMAI	GTCTC	5 cut(s)	8, 239, 521, 1447, 1814
BstNI	CCWGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
BstNSI	RCATGY	4 cut(s)	862, 1586, 1778, 2009
BstSCI	CCNGG	6 cut(s)	157, 506, 1437, 1510, 1555, 1719
BstSFI	CTRYAG	3 cut(s)	36, 1002, 1245
BstSLI	GKGCMC	2 cut(s)	1021, 2191
BstSNI	TACGTA	1 cut(s)	2364
BstUI	CGCG	1 cut(s)	2402
BstV2I	GAAGAC	1 cut(s)	1553
BstX2I	RGATCY	2 cut(s)	199, 757
BstYI	RGATCY	2 cut(s)	199, 757
BstZ17I	GTATAC	1 cut(s)	51
Bsu15I	ATCGAT	1 cut(s)	657
Bsu36I	CCTNAGG	1 cut(s)	2454
BsuI	GTATCC	1 cut(s)	2376
BsuTUI	ATCGAT	1 cut(s)	657
BtgI	CCRYGG	2 cut(s)	2319, 2412
BtgZI	GCGATG	1 cut(s)	2106
BtrI	CACGTC	2 cut(s)	644, 2040
BtsCI	GGATG	2 cut(s)	439, 2404
BtsI	GCAGTG	2 cut(s)	45, 1832
BtsIMutI	CAGTG	6 cut(s)	16, 45, 765, 1399, 1832, 2243
CaiI	CAGNNNCTG	3 cut(s)	767, 925, 1834
CciI	TCATGA	1 cut(s)	634
CfoI	GCGC	3 cut(s)	2317, 2329, 2404
Cfr10I	RCCGGY	1 cut(s)	2428
Cfr13I	GGNCC	3 cut(s)	874, 1215, 1263
ClaI	ATCGAT	1 cut(s)	657
CseI	GACGC	1 cut(s)	314
CsiI	ACCWGGT	2 cut(s)	1510, 1719
Csp6I	GTAC	5 cut(s)	856, 1538, 1702, 1778, 2266
CviQI	GTAC	5 cut(s)	856, 1538, 1702, 1778, 2266
DinI	GGCGCC	1 cut(s)	2328
EaeI	YGGCCR	4 cut(s)	968, 2171, 2204, 2240
Eam1104I	CTCTTC	5 cut(s)	519, 1143, 1445, 1923, 2021
EarI	CTCTTC	5 cut(s)	519, 1143, 1445, 1923, 2021
Eco105I	TACGTA	1 cut(s)	2364
Eco130I	CCWWGG	2 cut(s)	309, 492
Eco24I	GRGCYC	2 cut(s)	1887, 1901
Eco31I	GGTCTC	1 cut(s)	239
Eco47I	GGWCC	1 cut(s)	1263
Eco57I	CTGAAG	3 cut(s)	1014, 2064, 2430
Eco72I	CACGTG	2 cut(s)	1228, 1430
Eco81I	CCTNAGG	1 cut(s)	2454
EcoO109I	RGGNCCY	1 cut(s)	1215
EcoRII	CCWGG	6 cut(s)	157, 506, 1437, 1510, 1555, 1719
EcoT14I	CCWWGG	2 cut(s)	309, 492
EcoT22I	ATGCAT	3 cut(s)	190, 1385, 2275
EcoT38I	GRGCYC	2 cut(s)	1887, 1901
EgeI	GGCGCC	1 cut(s)	2328
EheI	GGCGCC	1 cut(s)	2328
ErhI	CCWWGG	2 cut(s)	309, 492
FaqI	GGGAC	2 cut(s)	2244, 2369
FauI	CCCGC	2 cut(s)	2134, 2394
FauNDI	CATATG	3 cut(s)	241, 751, 2275
FbaI	TGATCA	1 cut(s)	1849
FblI	GTMKAC	1 cut(s)	50
FokI	GGATG	2 cut(s)	446, 2391
FriOI	GRGCYC	2 cut(s)	1887, 1901
FspBI	CTAG	7 cut(s)	276, 330, 647, 1274, 1746, 1911, 2096
GlaI	GCGC	3 cut(s)	2316, 2328, 2403
GsaI	CCCAGC	1 cut(s)	1890
GsuI	CTGGAG	1 cut(s)	387
HaeII	RGCGCY	2 cut(s)	2318, 2330
HapII	CCGG	2 cut(s)	1460, 2429
HgaI	GACGC	1 cut(s)	314
HhaI	GCGC	3 cut(s)	2317, 2329, 2404
Hin1I	GRCGYC	2 cut(s)	306, 2327
Hin6I	GCGC	3 cut(s)	2315, 2327, 2402
HinP1I	GCGC	3 cut(s)	2315, 2327, 2402
HindIII	AAGCTT	2 cut(s)	2103, 2199
HinfI	GANTC	7 cut(s)	314, 338, 1178, 1366, 1485, 1802, 1859
HpaII	CCGG	2 cut(s)	1460, 2429
HphI	GGTGA	2 cut(s)	188, 1129
Hpy166II	GTNNAC	4 cut(s)	51, 1019, 1713, 2189
Hpy188I	TCNGA	9 cut(s)	130, 567, 979, 1027, 1066, 1546, 1894, 2044, 2083
Hpy8I	GTNNAC	4 cut(s)	51, 1019, 1713, 2189
HpyAV	CCTTC	3 cut(s)	768, 1984, 2367
HpyCH4IV	ACGT	8 cut(s)	643, 682, 1227, 1429, 1679, 2039, 2056, 2363
HpySE526I	ACGT	8 cut(s)	643, 682, 1227, 1429, 1679, 2039, 2056, 2363
Hsp92I	GRCGYC	2 cut(s)	306, 2327
HspAI	GCGC	3 cut(s)	2315, 2327, 2402
KasI	GGCGCC	1 cut(s)	2326
Ksp22I	TGATCA	1 cut(s)	1849
LguI	GCTCTTC	2 cut(s)	1923, 2021
LmnI	GCTCC	4 cut(s)	1763, 2031, 2144, 2437
LweI	GCATC	8 cut(s)	76, 262, 424, 568, 1455, 1950, 1963, 2413
MabI	ACCWGGT	2 cut(s)	1510, 1719
MaeI	CTAG	7 cut(s)	276, 330, 647, 1274, 1746, 1911, 2096
MaeII	ACGT	8 cut(s)	643, 682, 1227, 1429, 1679, 2039, 2056, 2363
MaeIII	GTNAC	6 cut(s)	230, 265, 697, 1680, 2396, 2471
MflI	RGATCY	2 cut(s)	199, 757
MhlI	GDGCHC	6 cut(s)	1021, 1519, 1768, 1887, 1901, 2191
MlsI	TGGCCA	3 cut(s)	2173, 2206, 2242
MluNI	TGGCCA	3 cut(s)	2173, 2206, 2242
Mly113I	GGCGCC	1 cut(s)	2327
MlyI	GAGTC	2 cut(s)	323, 1811
MmeI	TCCRAC	4 cut(s)	241, 784, 1104, 2432
Mox20I	TGGCCA	3 cut(s)	2173, 2206, 2242
Mph1103I	ATGCAT	3 cut(s)	190, 1385, 2275
MroXI	GAANNNNTTC	2 cut(s)	379, 1056
MscI	TGGCCA	3 cut(s)	2173, 2206, 2242
MseI	TTAA	4 cut(s)	518, 948, 1106, 1959
Msp20I	TGGCCA	3 cut(s)	2173, 2206, 2242
MspA1I	CMGCKG	3 cut(s)	257, 2177, 2246
MspI	CCGG	2 cut(s)	1460, 2429
MspR9I	CCNGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
Mva1269I	GAATGC	3 cut(s)	124, 1669, 1738
MvaI	CCWGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
MvnI	CGCG	1 cut(s)	2402
NarI	GGCGCC	1 cut(s)	2327
NdeI	CATATG	3 cut(s)	241, 751, 2275
NheI	GCTAGC	2 cut(s)	1910, 2095
NlaIV	GGNNCC	5 cut(s)	201, 875, 1331, 2328, 2433
NmeAIII	GCCGAG	1 cut(s)	1141
NmuCI	GTSAC	1 cut(s)	265
NsiI	ATGCAT	3 cut(s)	190, 1385, 2275
NspI	RCATGY	4 cut(s)	862, 1586, 1778, 2009
PaeI	GCATGC	1 cut(s)	1586
PagI	TCATGA	1 cut(s)	634
PciSI	GCTCTTC	2 cut(s)	1923, 2021
PctI	GAATGC	3 cut(s)	124, 1669, 1738
PdmI	GAANNNNTTC	2 cut(s)	379, 1056
PfeI	GAWTC	5 cut(s)	338, 1178, 1366, 1485, 1859
PflMI	CCANNNNNTGG	1 cut(s)	260
PleI	GAGTC	2 cut(s)	322, 1810
PluTI	GGCGCC	1 cut(s)	2330
PmaCI	CACGTG	2 cut(s)	1228, 1430
PmlI	CACGTG	2 cut(s)	1228, 1430
PpsI	GAGTC	2 cut(s)	322, 1810
Ppu21I	YACGTR	3 cut(s)	1228, 1430, 2364
Psp6I	CCWGG	6 cut(s)	157, 506, 1437, 1510, 1555, 1719
PspCI	CACGTG	2 cut(s)	1228, 1430
PspFI	CCCAGC	1 cut(s)	1886
PspGI	CCWGG	6 cut(s)	157, 506, 1437, 1510, 1555, 1719
PspN4I	GGNNCC	5 cut(s)	201, 875, 1331, 2328, 2433
PspPI	GGNCC	3 cut(s)	874, 1215, 1263
PsrI	GAACNNNNNNTAC	2 cut(s)	1694, 1726
PstI	CTGCAG	2 cut(s)	40, 1006
PstNI	CAGNNNCTG	3 cut(s)	767, 925, 1834
PsuI	RGATCY	2 cut(s)	199, 757
PvuII	CAGCTG	3 cut(s)	257, 2177, 2246
RsaI	GTAC	5 cut(s)	857, 1539, 1703, 1779, 2267
RsaNI	GTAC	5 cut(s)	856, 1538, 1702, 1778, 2266
SapI	GCTCTTC	2 cut(s)	1923, 2021
SaqAI	TTAA	4 cut(s)	518, 948, 1106, 1959
Sau96I	GGNCC	3 cut(s)	874, 1215, 1263
ScaI	AGTACT	1 cut(s)	1703
SchI	GAGTC	2 cut(s)	323, 1811
ScrFI	CCNGG	6 cut(s)	159, 508, 1439, 1512, 1557, 1721
SduI	GDGCHC	6 cut(s)	1021, 1519, 1768, 1887, 1901, 2191
SexAI	ACCWGGT	2 cut(s)	1510, 1719
SfaNI	GCATC	8 cut(s)	76, 262, 424, 568, 1455, 1950, 1963, 2413
SfcI	CTRYAG	3 cut(s)	36, 1002, 1245
SfoI	GGCGCC	1 cut(s)	2328
SinI	GGWCC	1 cut(s)	1263
SmlI	CTYRAG	6 cut(s)	321, 1300, 1518, 1769, 1799, 2196
SmoI	CTYRAG	6 cut(s)	321, 1300, 1518, 1769, 1799, 2196
SnaBI	TACGTA	1 cut(s)	2364
SphI	GCATGC	1 cut(s)	1586
SspDI	GGCGCC	1 cut(s)	2326
SspI	AATATT	2 cut(s)	100, 903
SspMI	CTAG	7 cut(s)	276, 330, 647, 1274, 1746, 1911, 2096
StyD4I	CCNGG	6 cut(s)	157, 506, 1437, 1510, 1555, 1719
StyI	CCWWGG	2 cut(s)	309, 492
TaiI	ACGT	8 cut(s)	646, 685, 1230, 1432, 1682, 2042, 2059, 2366
TaqI	TCGA	8 cut(s)	453, 657, 1176, 1369, 1483, 1818, 2002, 2135
TatI	WGTACW	4 cut(s)	855, 1537, 1701, 1777
TauI	GCSGC	2 cut(s)	879, 1234
TfiI	GAWTC	5 cut(s)	338, 1178, 1366, 1485, 1859
Tru1I	TTAA	4 cut(s)	518, 948, 1106, 1959
Tru9I	TTAA	4 cut(s)	518, 948, 1106, 1959
TscAI	CASTG	6 cut(s)	16, 45, 772, 1399, 1839, 2243
TseFI	GTSAC	1 cut(s)	265
Tsp45I	GTSAC	1 cut(s)	265
TspDTI	ATGAA	8 cut(s)	312, 362, 372, 693, 1338, 1851, 1867, 2008
TspGWI	ACGGA	1 cut(s)	588
TspRI	CASTG	6 cut(s)	16, 45, 772, 1399, 1839, 2243
Van91I	CCANNNNNTGG	1 cut(s)	260
VneI	GTGCAC	2 cut(s)	1017, 2187
VpaK11BI	GGWCC	1 cut(s)	1263
XapI	RAATTY	2 cut(s)	561, 1752
XceI	RCATGY	4 cut(s)	862, 1586, 1778, 2009
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1399, 2174
XmiI	GTMKAC	1 cut(s)	50
XmnI	GAANNNNTTC	2 cut(s)	379, 1056
XspI	CTAG	7 cut(s)	276, 330, 647, 1274, 1746, 1911, 2096
ZrmI	AGTACT	1 cut(s)	1703
Zsp2I	ATGCAT	3 cut(s)	190, 1385, 2275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.