Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0002317

Physical Location & Seq

Genomic Coordinates Reverse (-)

28778631 .. 28788347

Gene Structure 9717 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0182200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2355 bp

ATGCCGAGGAAGAGTAAAGCAAAGAAGAAGGAAGAGATTTGCGAGGAGGTTTGCTTCTATTGCAAAGACGGAGGACTCCTCTTGGTCTGCGACTACAAGGGTTGTCTTAAAGCTTATCATCCTGAATGTGCGGGAAAAGATGACTCTTTTGTGGAGACTGCACGGCGCTGGAACTGCAAATGGCATTCTTGCTTCATGTGCCATAAGGCTGCAAAGTTTCAATGCTTTTGCTGCCCACAAGCTGTATGCGGACGCTGCCTCTGTGACTCTGAGTTTGCGGTTATTAAAGTGGATAGAGGATTTTGCAATCATTGCTTGAAGCTTGCTGTACTCATAGAAGAAAACTTGGATGCTGATTCTGACGGGGGCAGAGTTGACTTCAAAGATATAAACACATTCGAGTACTTATTTAAGGAGTATTGGCAGGTAATGAAAAAGAAAGAACGCTTGTCTGCAGAACATGTCTACTCTGCGAAAAAGTTATTAAAGAAGGGAAAGAACTACGTGCGTGACTTTGATTCGAGTATAATTTATAAGAAAGAAGAAAAATTTTCAGAATCCGATGAAGAAAGTGACCTTCAGGTGTCTGATTATGATGGAGTAGAAAGTGGAGAAGAATACATGCCAGTTGCCAAAAAGAAGAGATCCAAGGGAAAACAGTGTGCAGTGAAAAGAAAAGTAGAAGCAGAGAAACGAGAATTTATGGGATGGGGATCAAAGTCTCTCCTAGAATTCCTTGCTTCTATGGGTAAAGATACATCCAAGGAGTTGTCACAGTATGAAGTCACCTCTGTGGTTACAGACTATTGTAAAGAAAACAAGCTTTTTGACCCAGAAAAGAAGAAGAAAGTAATTTGCGATGCAACCTTGCAGTCTCTTTTAGGGAGGAAAACATTGAGTAAAAATAGTATATATAACCTTCTGACTTCCCATTTTGCTGACAATATGGAAGAGTCAACGGAAGGGGATGAGAGTGGAAGTAGTTCAGAAGACAAAGATGAGAGTTTTATGGGTACCTGTAAAAGGCAGATCAAGTTGAGCCCTAAAGTACCAGATGTGCAGAAAAGCTGTTTTGCTTCCATAGTTCCTGAAAACATCAAGCTTGTGTACCTGAAGAGGAGCTTAGTGGAGGAATTGTTAAAGCAACCTGAGACTTTTGGTGAGAAAGTAACAGGATGTTTTGTGAGAGTCAAATCTGACCCAAATGATTATTCACAGAAAAATTCTCACCAGCTCTTGCAAATTAAAGGTATACAGGAAATTTTGAGGAATGGCAGAGAGGATACAGAAATACTTTTGCAATTTTTCAATAATTTTAAAGATTTACCTGTTTCCAAGCTGTCAGATGATGACTTCTGTAAGGAAGAATGTGAAGATTTGCATCAGAGAGTGAAAGATGGCCTCCTTAAAAAGCCAACTATTGTGGAGCTTGAACAGAAGGCTAGAAGTCTACATGAGAATATAACAAAGCACTGGCTTGCAAGAGAACTGACTCTGTTGCAGAAGCGCATTGACCTGGCAAATGAAAAAGGATGGAGGAGAGAGCTTGATGCGTACATGAATAAGAAGCTGCTACTTGAAACACCATCAGAACAATCACGGCTGTTAAATGAGGTTCCGGAAGTAATTGCAGCCATAGAAAAATGTGATCCTGTTCTTGAGTACTCCAGAAGCCAGGATAAACAAGGAAATGATGGCTCTTCCGAATCTGCCCCCAGAGAATCTCCTCAAACTCCCGGCACTGCTCTAGCCAATGAAACCTCATGGCGTGCAAATGGCAGAACAGATCCTGCAGGTTCACAGCTAGACCAGGAGAGAGACCTGGAGGGTGCAACAGCTAAACGTAAGAGAAAGCAGGCTTCATCAAACTTTGAACAGAGCTCTAGTATAGACGGTTCAAATGGTGACCATGAGAGAGACCTGGAAGATGCAAAGGATAGATCTAAGAGAAAGAAGGTTCCTACAATTCATGACTGCAAGCAGTCTCCTTGCATTCCATCTGGTCAGGCAGCAGAACTAGTGGAACCTAAAGTAGCTCGACTAGAAAGGATGAACTGTGAAAGCACATCTGAAGGTAAGGTAATTGAGTTGAGCGATGACGATGAAGATGTAATGGTTTCAGTTACAACACCTGCACTTGAAGTCCCAGGTTCTGATTTATGGTATTGTATGAGTCCACTTGGTGAAATGAGGGGCCCCTATAAATTGTCATTGCTAAAGCTATGGCGCGTCTCGAGTGGCAATGAATTAAGATACAAGGTTTGGAGAGTGGGTCAGAGCAAAGACGAGGCAATCTTTCTCAATGATGCCATTCATGGAAATTTTCCACCATGTAGAAATGCTTCCAAATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000243 GO:0000245 GO:0000375 GO:0000377 GO:0000395 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005685 GO:0006139 GO:0006376 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0065003 GO:0071004 GO:0071010 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0120114 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

784

Amino Acids

89.2

Weight (kDa)

6.33

Isoelectric Point (pI)

53.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SWIB	PF02201	240 - 312	2.5e-12	SWIB/MDM2 domain
SWIB_eIF2D	PF26291	241 - 297	3.5e-07	eIF2D, SWIB domain
Plus-3	PF03126	365 - 464	7.3e-18	Plus-3 domain
NERD_plant	PF25980	487 - 535	1.2e-21	Plant NERD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04080 AT1G04080 AT1G04080 AT1G04080 AT1G04080
fragaria_vesca	FvH4_2g20550
malus_domestica	MD00G1060100.v1.1
prunus_persica	Prupe.8G215900_v2.0.a1 Prupe.8G215900_v2.0.a1 Prupe.8G215900_v2.0.a1 Prupe.8G215900_v2.0.a1
pyrus_communis	pycom05g17390 pycom10g15270
rosa_chinensis	RchiOBHm_Chr1g0327141 RchiOBHm_Chr6g0282561 RchiOBHm_Chr6g0286711
rosa_laevigata	RLG00000007391 RLG00000012550 RLG00000030995
rosa_multiflora	Rmu_co8283097.1_g000001 Rmu_co8332197.1_g000001 Rmu_sc0001468.1_g000022
rosa_roxburghii	Rroxscaffold_1G00034270 Rroxscaffold_1G00060390 Rroxscaffold_7G00182090
rosa_rugosa	Rorug05G0468800 Rorug06G0182100 Rorug06G0182200
rosa_samantha	Rh2CG306300 Rh5BG355000 Rh5BG355100 Rh5BG357900 Rh6AG023000 Rh6AG294900 Rh6BG204700 Rh6BG298100 Rh6BG298200 Rh6CG298600 Rh6DG290600
rosa_wichuraiana	Rw1G013170 Rw5G035150 Rw6G025370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	534
AarI	CACCTGC	1 cut(s)	2140
Acc36I	ACCTGC	3 cut(s)	415, 1784, 2140
Acc65I	GGTACC	1 cut(s)	1013
AccB1I	GGYRCC	1 cut(s)	1013
AccI	GTMKAC	3 cut(s)	465, 1252, 1450
AccII	CGCG	1 cut(s)	2229
AccIII	TCCGGA	1 cut(s)	1618
AciI	CCGC	3 cut(s)	131, 249, 278
AclWI	GGATC	4 cut(s)	639, 721, 1643, 1781
AcsI	RAATTY	6 cut(s)	548, 698, 731, 1222, 1260, 2320
AcuI	CTGAAG	3 cut(s)	563, 1133, 2091
AdeI	CACNNNGTG	1 cut(s)	2183
AfaI	GTAC	7 cut(s)	330, 404, 1015, 1050, 1109, 1556, 1664
AfiI	CCNNNNNNNGG	1 cut(s)	1023
AflIII	ACRYGT	1 cut(s)	460
AgsI	TTSAA	9 cut(s)	221, 319, 382, 1309, 1433, 1580, 1874, 1899, 2141
AhlI	ACTAGT	1 cut(s)	2017
AjnI	CCWGG	6 cut(s)	1515, 1674, 1809, 1821, 1920, 2146
AjuI	GAANNNNNNNTTGG	2 cut(s)	1195, 1227
AleI	CACNNNNGTG	1 cut(s)	791
Alw21I	GWGCWC	1 cut(s)	1883
Alw26I	GTCTC	8 cut(s)	149, 726, 879, 1145, 1812, 1911, 1989, 2236
AlwI	GGATC	4 cut(s)	639, 721, 1643, 1781
AlwNI	CAGNNNCTG	2 cut(s)	1790, 2153
Ama87I	CYCGRG	1 cut(s)	2233
Aor13HI	TCCGGA	1 cut(s)	1618
AoxI	GGCC	2 cut(s)	1399, 2194
ApaI	GGGCCC	1 cut(s)	2198
ApeKI	GCWGC	6 cut(s)	209, 231, 255, 1570, 1631, 2009
ApoI	RAATTY	6 cut(s)	548, 698, 731, 1222, 1260, 2320
ArsI	GACNNNNNNTTYG	2 cut(s)	257, 289
Asp700I	GAANNNNTTC	2 cut(s)	982, 2341
Asp718I	GGTACC	1 cut(s)	1013
AspLEI	GCGC	3 cut(s)	168, 1509, 2229
AspS9I	GGNCC	2 cut(s)	2194, 2195
AsuC2I	CCSGG	1 cut(s)	1737
AsuHPI	GGTGA	5 cut(s)	778, 1172, 1220, 1916, 2195
AvaI	CYCGRG	1 cut(s)	2233
BaeGI	GKGCMC	1 cut(s)	2198
BanI	GGYRCC	1 cut(s)	1013
BanII	GRGCYC	3 cut(s)	1043, 1883, 2198
BarI	GAAGNNNNNNTAC	1 cut(s)	2326
BbsI	GAAGAC	1 cut(s)	996
Bbv12I	GWGCWC	1 cut(s)	1883
BbvI	GCAGC	6 cut(s)	196, 218, 242, 1557, 1643, 2021
BccI	CCATC	7 cut(s)	590, 702, 1391, 1527, 1594, 1688, 2005
BceAI	ACGGC	2 cut(s)	179, 1616
BciT130I	CCWGG	6 cut(s)	1517, 1676, 1811, 1823, 1922, 2148
BciVI	GTATCC	1 cut(s)	1276
BcnI	CCSGG	1 cut(s)	1737
BcoDI	GTCTC	8 cut(s)	149, 726, 879, 1145, 1812, 1911, 1989, 2236
BcuI	ACTAGT	1 cut(s)	2017
BfaI	CTAG	7 cut(s)	728, 1443, 1748, 1805, 1884, 2018, 2042
BfmI	CTRYAG	2 cut(s)	453, 1791
BfoI	RGCGCY	1 cut(s)	169
BfuAI	ACCTGC	3 cut(s)	415, 1784, 2140
BfuI	GTATCC	1 cut(s)	1276
BglII	AGATCT	1 cut(s)	1940
BisI	GCNGC	6 cut(s)	210, 232, 256, 1571, 1632, 2010
BlsI	GCNGC	6 cut(s)	211, 233, 257, 1572, 1633, 2011
BmcAI	AGTACT	2 cut(s)	404, 1664
Bme1390I	CCNGG	7 cut(s)	1517, 1676, 1737, 1811, 1823, 1922, 2148
BmeT110I	CYCGRG	1 cut(s)	2233
BmgT120I	GGNCC	2 cut(s)	2194, 2195
BmiI	GGNNCC	7 cut(s)	1015, 1617, 1959, 2025, 2195, 2196, 2197
BmrFI	CCNGG	7 cut(s)	1517, 1676, 1737, 1811, 1823, 1922, 2148
BmsI	GCATC	6 cut(s)	340, 850, 1390, 1540, 1918, 2296
BpiI	GAAGAC	1 cut(s)	996
BplI	GAGNNNNNCTC	2 cut(s)	63, 95
BpmI	CTGGAG	2 cut(s)	1651, 1844
BpuEI	CTTGAG	1 cut(s)	1679
BpuMI	CCSGG	1 cut(s)	1737
BsaAI	YACGTR	1 cut(s)	505
BsaBI	GATNNNNATC	2 cut(s)	712, 1380
BsaI	GGTCTC	2 cut(s)	1812, 1911
BsaJI	CCNNGG	4 cut(s)	5, 648, 762, 2146
BsaWI	WCCGGW	1 cut(s)	1618
BsaXI	ACNNNNNCTCC	4 cut(s)	1121, 1151, 2257, 2287
Bsc4I	CCNNNNNNNGG	1 cut(s)	1023
Bse1I	ACTGG	2 cut(s)	626, 1478
Bse3DI	GCAATG	3 cut(s)	310, 2210, 2248
Bse8I	GATNNNNATC	2 cut(s)	712, 1380
BseAI	TCCGGA	1 cut(s)	1618
BseBI	CCWGG	6 cut(s)	1517, 1676, 1811, 1823, 1922, 2148
BseDI	CCNNGG	4 cut(s)	5, 648, 762, 2146
BseGI	GGATG	8 cut(s)	118, 355, 713, 758, 973, 1181, 1538, 2055
BseJI	GATNNNNATC	2 cut(s)	712, 1380
BseLI	CCNNNNNNNGG	1 cut(s)	1023
BseMI	GCAATG	3 cut(s)	310, 2210, 2248
BseMII	CTCAG	2 cut(s)	261, 1140
BseNI	ACTGG	2 cut(s)	626, 1478
BseRI	GAGGAG	5 cut(s)	59, 68, 1132, 1552, 1716
BseSI	GKGCMC	1 cut(s)	2198
BseXI	GCAGC	6 cut(s)	196, 218, 242, 1557, 1643, 2021
BsgI	GTGCAG	4 cut(s)	144, 684, 1079, 2118
Bsh1236I	CGCG	1 cut(s)	2229
BshFI	GGCC	2 cut(s)	1401, 2196
BshNI	GGYRCC	1 cut(s)	1013
BsiHKAI	GWGCWC	1 cut(s)	1883
BsiHKCI	CYCGRG	1 cut(s)	2233
BsiSI	CCGG	2 cut(s)	1619, 1737
BslFI	GGGAC	1 cut(s)	2129
BslI	CCNNNNNNNGG	1 cut(s)	1023
BsmAI	GTCTC	8 cut(s)	149, 726, 879, 1145, 1812, 1911, 1989, 2236
BsmBI	CGTCTC	1 cut(s)	2236
BsmFI	GGGAC	1 cut(s)	2129
BsmI	GAATGC	2 cut(s)	184, 1992
BsnI	GGCC	2 cut(s)	1401, 2196
Bso31I	GGTCTC	2 cut(s)	1812, 1911
BsoBI	CYCGRG	1 cut(s)	2233
Bsp120I	GGGCCC	1 cut(s)	2194
Bsp1286I	GDGCHC	3 cut(s)	1043, 1883, 2198
Bsp13I	TCCGGA	1 cut(s)	1618
Bsp143I	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
BspACI	CCGC	3 cut(s)	131, 249, 278
BspANI	GGCC	2 cut(s)	1401, 2196
BspCNI	CTCAG	2 cut(s)	262, 1141
BspEI	TCCGGA	1 cut(s)	1618
BspFNI	CGCG	1 cut(s)	2229
BspHI	TCATGA	1 cut(s)	1969
BspLI	GGNNCC	7 cut(s)	1015, 1617, 1959, 2025, 2195, 2196, 2197
BspMAI	CTGCAG	2 cut(s)	457, 1795
BspMI	ACCTGC	3 cut(s)	415, 1784, 2140
BspPI	GGATC	4 cut(s)	639, 721, 1643, 1781
BspQI	GCTCTTC	1 cut(s)	1705
BspT107I	GGYRCC	1 cut(s)	1013
BspTNI	GGTCTC	2 cut(s)	1812, 1911
BsrDI	GCAATG	3 cut(s)	310, 2210, 2248
BsrI	ACTGG	2 cut(s)	626, 1478
BssECI	CCNNGG	4 cut(s)	5, 648, 762, 2146
BssMI	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
BssNAI	GTATAC	1 cut(s)	1253
BssT1I	CCWWGG	2 cut(s)	648, 762
Bst1107I	GTATAC	1 cut(s)	1253
Bst2UI	CCWGG	6 cut(s)	1517, 1676, 1811, 1823, 1922, 2148
Bst4CI	ACNGT	4 cut(s)	660, 777, 1895, 2057
Bst6I	CTCTTC	6 cut(s)	5, 27, 635, 945, 1109, 1705
BstAPI	GCANNNNNTGC	1 cut(s)	312
BstBAI	YACGTR	1 cut(s)	505
BstC8I	GCNNGC	5 cut(s)	324, 1479, 1770, 1857, 1979
BstDEI	CTNAG	4 cut(s)	270, 1123, 1149, 1944
BstEII	GGTNACC	1 cut(s)	1904
BstENI	CCTNNNNNAGG	1 cut(s)	1021
BstF5I	GGATG	8 cut(s)	118, 355, 713, 758, 973, 1181, 1538, 2055
BstFNI	CGCG	1 cut(s)	2229
BstH2I	RGCGCY	1 cut(s)	169
BstHHI	GCGC	3 cut(s)	168, 1509, 2229
BstKTI	GATC	6 cut(s)	647, 716, 1032, 1651, 1789, 1943
BstMAI	GTCTC	8 cut(s)	149, 726, 879, 1145, 1812, 1911, 1989, 2236
BstMBI	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
BstMWI	GCNNNNNNNGC	6 cut(s)	60, 174, 198, 231, 255, 312
BstNI	CCWGG	6 cut(s)	1517, 1676, 1811, 1823, 1922, 2148
BstNSI	RCATGY	2 cut(s)	464, 625
BstPI	GGTNACC	1 cut(s)	1904
BstSCI	CCNGG	7 cut(s)	1515, 1674, 1735, 1809, 1821, 1920, 2146
BstSFI	CTRYAG	2 cut(s)	453, 1791
BstSLI	GKGCMC	1 cut(s)	2198
BstUI	CGCG	1 cut(s)	2229
BstV1I	GCAGC	6 cut(s)	196, 218, 242, 1557, 1643, 2021
BstV2I	GAAGAC	1 cut(s)	996
BstX2I	RGATCY	3 cut(s)	644, 1786, 1940
BstYI	RGATCY	3 cut(s)	644, 1786, 1940
BstZ17I	GTATAC	1 cut(s)	1253
BsuI	GTATCC	1 cut(s)	1276
BsuRI	GGCC	2 cut(s)	1401, 2196
BtgZI	GCGATG	2 cut(s)	873, 2109
BtsCI	GGATG	8 cut(s)	118, 355, 713, 758, 973, 1181, 1538, 2055
BtsI	GCAGTG	2 cut(s)	672, 1740
BtsIMutI	CAGTG	4 cut(s)	665, 672, 1471, 1740
BveI	ACCTGC	3 cut(s)	415, 1784, 2140
Cac8I	GCNNGC	5 cut(s)	324, 1479, 1770, 1857, 1979
CaiI	CAGNNNCTG	2 cut(s)	1790, 2153
CciI	TCATGA	1 cut(s)	1969
CfoI	GCGC	3 cut(s)	168, 1509, 2229
Cfr13I	GGNCC	2 cut(s)	2194, 2195
CseI	GACGC	2 cut(s)	261, 2218
Csp6I	GTAC	7 cut(s)	329, 403, 1014, 1049, 1108, 1555, 1663
CspCI	CAANNNNNGTGG	2 cut(s)	1404, 1439
CviQI	GTAC	7 cut(s)	329, 403, 1014, 1049, 1108, 1555, 1663
DdeI	CTNAG	4 cut(s)	270, 1123, 1149, 1944
DpnI	GATC	6 cut(s)	646, 715, 1031, 1650, 1788, 1942
DpnII	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
DraI	TTTAAA	1 cut(s)	1318
DraIII	CACNNNGTG	1 cut(s)	2183
Eam1104I	CTCTTC	6 cut(s)	5, 27, 635, 945, 1109, 1705
EarI	CTCTTC	6 cut(s)	5, 27, 635, 945, 1109, 1705
Ecl136II	GAGCTC	1 cut(s)	1881
Eco130I	CCWWGG	2 cut(s)	648, 762
Eco24I	GRGCYC	3 cut(s)	1043, 1883, 2198
Eco31I	GGTCTC	2 cut(s)	1812, 1911
Eco53kI	GAGCTC	1 cut(s)	1881
Eco57I	CTGAAG	3 cut(s)	563, 1133, 2091
Eco88I	CYCGRG	1 cut(s)	2233
Eco91I	GGTNACC	1 cut(s)	1904
EcoICRI	GAGCTC	1 cut(s)	1881
EcoNI	CCTNNNNNAGG	1 cut(s)	1021
EcoO109I	RGGNCCY	2 cut(s)	2194, 2195
EcoO65I	GGTNACC	1 cut(s)	1904
EcoRI	GAATTC	1 cut(s)	731
EcoRII	CCWGG	6 cut(s)	1515, 1674, 1809, 1821, 1920, 2146
EcoT14I	CCWWGG	2 cut(s)	648, 762
EcoT38I	GRGCYC	3 cut(s)	1043, 1883, 2198
ErhI	CCWWGG	2 cut(s)	648, 762
Esp3I	CGTCTC	1 cut(s)	2236
FalI	AAGNNNNNCTT	2 cut(s)	1106, 1138
FaqI	GGGAC	1 cut(s)	2129
FauI	CCCGC	1 cut(s)	124
FblI	GTMKAC	3 cut(s)	465, 1252, 1450
Fnu4HI	GCNGC	6 cut(s)	210, 232, 256, 1571, 1632, 2010
FokI	GGATG	8 cut(s)	105, 362, 720, 745, 980, 1188, 1545, 2062
FriOI	GRGCYC	3 cut(s)	1043, 1883, 2198
Fsp4HI	GCNGC	6 cut(s)	210, 232, 256, 1571, 1632, 2010
FspBI	CTAG	7 cut(s)	728, 1443, 1748, 1805, 1884, 2018, 2042
GlaI	GCGC	3 cut(s)	167, 1508, 2228
GluI	GCNGC	6 cut(s)	210, 232, 256, 1571, 1632, 2010
GsuI	CTGGAG	2 cut(s)	1651, 1844
HaeII	RGCGCY	1 cut(s)	169
HaeIII	GGCC	2 cut(s)	1401, 2196
HapII	CCGG	2 cut(s)	1619, 1737
HgaI	GACGC	2 cut(s)	261, 2218
HhaI	GCGC	3 cut(s)	168, 1509, 2229
Hin6I	GCGC	3 cut(s)	166, 1507, 2227
HinP1I	GCGC	3 cut(s)	166, 1507, 2227
HincII	GTYRAC	2 cut(s)	376, 957
HindII	GTYRAC	2 cut(s)	376, 957
HindIII	AAGCTT	4 cut(s)	111, 320, 821, 1100
HpaII	CCGG	2 cut(s)	1619, 1737
HphI	GGTGA	5 cut(s)	778, 1172, 1220, 1916, 2195
Hpy166II	GTNNAC	8 cut(s)	376, 466, 957, 1108, 1253, 1451, 1799, 2177
Hpy188III	TCNNGA	7 cut(s)	122, 1088, 1619, 1658, 1668, 1970, 2233
Hpy8I	GTNNAC	8 cut(s)	376, 466, 957, 1108, 1253, 1451, 1799, 2177
HpyAV	CCTTC	8 cut(s)	22, 484, 587, 929, 956, 1432, 1948, 2066
HpyCH4III	ACNGT	4 cut(s)	660, 777, 1895, 2057
HpyCH4IV	ACGT	2 cut(s)	504, 1843
HpyF10VI	GCNNNNNNNGC	6 cut(s)	60, 174, 198, 231, 255, 312
HpyF3I	CTNAG	4 cut(s)	270, 1123, 1149, 1944
HpySE526I	ACGT	2 cut(s)	504, 1843
HspAI	GCGC	3 cut(s)	166, 1507, 2227
Kpn2I	TCCGGA	1 cut(s)	1618
KpnI	GGTACC	1 cut(s)	1017
Kzo9I	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
LguI	GCTCTTC	1 cut(s)	1705
LmnI	GCTCC	2 cut(s)	1119, 1426
Lsp1109I	GCAGC	6 cut(s)	196, 218, 242, 1557, 1643, 2021
LweI	GCATC	6 cut(s)	340, 850, 1390, 1540, 1918, 2296
MaeI	CTAG	7 cut(s)	728, 1443, 1748, 1805, 1884, 2018, 2042
MaeII	ACGT	2 cut(s)	504, 1843
MaeIII	GTNAC	9 cut(s)	263, 509, 572, 771, 784, 796, 1168, 1904, 2122
MalI	GATC	6 cut(s)	646, 715, 1031, 1650, 1788, 1942
MboI	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
MflI	RGATCY	3 cut(s)	644, 1786, 1940
MhlI	GDGCHC	3 cut(s)	1043, 1883, 2198
MlyI	GAGTC	7 cut(s)	69, 137, 260, 962, 1197, 1486, 2182
MroI	TCCGGA	1 cut(s)	1618
MroXI	GAANNNNTTC	2 cut(s)	982, 2341
MslI	CAYNNNNRTG	1 cut(s)	791
MspI	CCGG	2 cut(s)	1619, 1737
MspR9I	CCNGG	7 cut(s)	1517, 1676, 1737, 1811, 1823, 1922, 2148
Mva1269I	GAATGC	2 cut(s)	184, 1992
MvaI	CCWGG	6 cut(s)	1517, 1676, 1811, 1823, 1922, 2148
MvnI	CGCG	1 cut(s)	2229
MwoI	GCNNNNNNNGC	6 cut(s)	60, 174, 198, 231, 255, 312
NciI	CCSGG	1 cut(s)	1737
NdeII	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
NlaIV	GGNNCC	7 cut(s)	1015, 1617, 1959, 2025, 2195, 2196, 2197
NmeAIII	GCCGAG	1 cut(s)	30
NmuCI	GTSAC	6 cut(s)	263, 509, 572, 771, 784, 1904
NspI	RCATGY	2 cut(s)	464, 625
OliI	CACNNNNGTG	1 cut(s)	791
PaeR7I	CTCGAG	1 cut(s)	2233
PagI	TCATGA	1 cut(s)	1969
PaqCI	CACCTGC	1 cut(s)	2140
PciI	ACATGT	1 cut(s)	460
PciSI	GCTCTTC	1 cut(s)	1705
PctI	GAATGC	2 cut(s)	184, 1992
PdmI	GAANNNNTTC	2 cut(s)	982, 2341
PfeI	GAWTC	5 cut(s)	356, 518, 557, 1706, 1721
PkrI	GCNGC	6 cut(s)	211, 233, 257, 1572, 1633, 2011
PleI	GAGTC	7 cut(s)	69, 137, 260, 961, 1196, 1486, 2181
PpsI	GAGTC	7 cut(s)	69, 137, 260, 961, 1196, 1486, 2181
Ppu21I	YACGTR	1 cut(s)	505
PscI	ACATGT	1 cut(s)	460
PsiI	TTATAA	1 cut(s)	534
Psp124BI	GAGCTC	1 cut(s)	1883
Psp6I	CCWGG	6 cut(s)	1515, 1674, 1809, 1821, 1920, 2146
PspEI	GGTNACC	1 cut(s)	1904
PspGI	CCWGG	6 cut(s)	1515, 1674, 1809, 1821, 1920, 2146
PspN4I	GGNNCC	7 cut(s)	1015, 1617, 1959, 2025, 2195, 2196, 2197
PspOMI	GGGCCC	1 cut(s)	2194
PspPI	GGNCC	2 cut(s)	2194, 2195
PsrI	GAACNNNNNNTAC	2 cut(s)	1879, 1911
PstI	CTGCAG	2 cut(s)	457, 1795
PstNI	CAGNNNCTG	2 cut(s)	1790, 2153
PsuI	RGATCY	3 cut(s)	644, 1786, 1940
RsaI	GTAC	7 cut(s)	330, 404, 1015, 1050, 1109, 1556, 1664
RsaNI	GTAC	7 cut(s)	329, 403, 1014, 1049, 1108, 1555, 1663
RseI	CAYNNNNRTG	1 cut(s)	791
SacI	GAGCTC	1 cut(s)	1883
SapI	GCTCTTC	1 cut(s)	1705
SatI	GCNGC	6 cut(s)	210, 232, 256, 1571, 1632, 2010
Sau3AI	GATC	6 cut(s)	644, 713, 1029, 1648, 1786, 1940
Sau96I	GGNCC	2 cut(s)	2194, 2195
SbfI	CCTGCAGG	1 cut(s)	1795
ScaI	AGTACT	2 cut(s)	404, 1664
SchI	GAGTC	7 cut(s)	69, 137, 260, 962, 1197, 1486, 2182
ScrFI	CCNGG	7 cut(s)	1517, 1676, 1737, 1811, 1823, 1922, 2148
SdaI	CCTGCAGG	1 cut(s)	1795
SduI	GDGCHC	3 cut(s)	1043, 1883, 2198
SfaNI	GCATC	6 cut(s)	340, 850, 1390, 1540, 1918, 2296
SfcI	CTRYAG	2 cut(s)	453, 1791
Sfr274I	CTCGAG	1 cut(s)	2233
SlaI	CTCGAG	1 cut(s)	2233
SmiMI	CAYNNNNRTG	1 cut(s)	791
SmlI	CTYRAG	2 cut(s)	1658, 2233
SmoI	CTYRAG	2 cut(s)	1658, 2233
SpeI	ACTAGT	1 cut(s)	2017
Sse8387I	CCTGCAGG	1 cut(s)	1795
SsiI	CCGC	3 cut(s)	131, 249, 278
SspMI	CTAG	7 cut(s)	728, 1443, 1748, 1805, 1884, 2018, 2042
SstI	GAGCTC	1 cut(s)	1883
StyD4I	CCNGG	7 cut(s)	1515, 1674, 1735, 1809, 1821, 1920, 2146
StyI	CCWWGG	2 cut(s)	648, 762
TaaI	ACNGT	4 cut(s)	660, 777, 1895, 2057
TaiI	ACGT	2 cut(s)	507, 1846
TaqI	TCGA	4 cut(s)	399, 521, 2038, 2234
TatI	WGTACW	3 cut(s)	328, 402, 1662
TfiI	GAWTC	5 cut(s)	356, 518, 557, 1706, 1721
TscAI	CASTG	4 cut(s)	665, 672, 1478, 1747
TseFI	GTSAC	6 cut(s)	263, 509, 572, 771, 784, 1904
TseI	GCWGC	6 cut(s)	209, 231, 255, 1570, 1631, 2009
Tsp45I	GTSAC	6 cut(s)	263, 509, 572, 771, 784, 1904
TspGWI	ACGGA	2 cut(s)	84, 974
TspRI	CASTG	4 cut(s)	665, 672, 1478, 1747
XagI	CCTNNNNNAGG	1 cut(s)	1021
XapI	RAATTY	6 cut(s)	548, 698, 731, 1222, 1260, 2320
XceI	RCATGY	2 cut(s)	464, 625
XhoI	CTCGAG	1 cut(s)	2233
XmiI	GTMKAC	3 cut(s)	465, 1252, 1450
XmnI	GAANNNNTTC	2 cut(s)	982, 2341
XspI	CTAG	7 cut(s)	728, 1443, 1748, 1805, 1884, 2018, 2042
ZrmI	AGTACT	2 cut(s)	404, 1664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.