MD00G1203400.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001285
Physical Location & Seq
Forward (+)
48996527 .. 48999461
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1203400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGATGGAGTTCGGAGGAGGTGGCCGGAAGAGAGGCAGGCCGGAAGCTGCGCACTTTAATGGGAATGGCGGCTTCAAGAAATCCAAGCCAGAAATGGAGTCCTTTCAACCTGGTATAGGAAGCAAATCGAAGCCATGCACAAAGTTTTTCAGCACTTCCGGGTGCCCTTTTGGTGAAGGGTGTCACTTTTTGCATTATGTTCCTGGTGGCATCAAAGCAGTGTCTCAGATGACTGGTAGCAATCCAACCCATCCTCCTCCTCCTAGAAATTCAGCAGCCCCGCCATCATTTCCAGATGGTTCTTCTCCTCCAGCTGTCAAGACACGGTTGTGTAATAAGTACAACACAGTTGAAGGTTGCAAGTTTGGGGATAAATGCCATTTTGCCCATGGTGAGTGTGGAGCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 zf-CCCH zf-CCCH_2

Protein Analysis

137

Amino Acids

14.43

Weight (kDa)

9.3

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 107 - 132 7.1e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 162
AciI CCGC 2 cut(s) 69, 281
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 1 cut(s) 268
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 3 cut(s) 76, 107, 353
AjnI CCWGG 2 cut(s) 109, 202
AleI CACNNNNGTG 1 cut(s) 328
AluBI AGCT 3 cut(s) 47, 314, 404
AluI AGCT 3 cut(s) 47, 314, 404
Alw26I GTCTC 1 cut(s) 228
AoxI GGCC 2 cut(s) 22, 38
ApeKI GCWGC 2 cut(s) 47, 275
ApoI RAATTY 1 cut(s) 268
AspLEI GCGC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 2 cut(s) 185, 404
BaeGI GKGCMC 1 cut(s) 167
BanI GGYRCC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 34, 287
BccI CCATC 3 cut(s) 258, 290, 292
BciT130I CCWGG 2 cut(s) 111, 204
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 1 cut(s) 264
BisI GCNGC 3 cut(s) 48, 70, 276
BlsI GCNGC 3 cut(s) 49, 71, 277
Bme1390I CCNGG 3 cut(s) 111, 160, 204
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 3 cut(s) 111, 160, 204
BmsI GCATC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 160
BsaJI CCNNGG 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 238
BseBI CCWGG 2 cut(s) 111, 204
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 4 cut(s) 30, 246, 249, 297
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 2 cut(s) 34, 287
BshFI GGCC 2 cut(s) 24, 40
BshNI GGYRCC 1 cut(s) 162
BsiSI CCGG 3 cut(s) 25, 41, 159
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 228
BsnI GGCC 2 cut(s) 24, 40
Bsp1286I GDGCHC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 2 cut(s) 69, 281
BspANI GGCC 2 cut(s) 24, 40
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 162
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 2 cut(s) 111, 204
Bst4CI ACNGT 2 cut(s) 327, 349
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 225
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 228
BstNI CCWGG 2 cut(s) 111, 204
BstSCI CCNGG 3 cut(s) 109, 158, 202
BstSLI GKGCMC 1 cut(s) 167
BstV1I GCAGC 2 cut(s) 34, 287
BsuRI GGCC 2 cut(s) 24, 40
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 1 cut(s) 52
CsiI ACCWGGT 1 cut(s) 109
Csp6I GTAC 1 cut(s) 340
CviAII CATG 2 cut(s) 135, 389
CviJI RGCY 9 cut(s) 24, 40, 47, 72, 88, 133, 278, 314, 404
CviKI_1 RGCY 9 cut(s) 24, 40, 47, 72, 88, 133, 278, 314, 404
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoRII CCWGG 2 cut(s) 109, 202
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 2 cut(s) 138, 392
FaiI YATR 4 cut(s) 116, 136, 198, 390
FatI CATG 2 cut(s) 134, 388
FauI CCCGC 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 48, 70, 276
FokI GGATG 1 cut(s) 237
Fsp4HI GCNGC 3 cut(s) 48, 70, 276
FspBI CTAG 1 cut(s) 264
FspI TGCGCA 1 cut(s) 51
GlaI GCGC 1 cut(s) 51
GluI GCNGC 3 cut(s) 48, 70, 276
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 24, 40
HapII CCGG 3 cut(s) 25, 41, 159
HhaI GCGC 1 cut(s) 52
Hin1II CATG 2 cut(s) 138, 392
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 1 cut(s) 98
HpaII CCGG 3 cut(s) 25, 41, 159
HphI GGTGA 2 cut(s) 185, 404
Hpy188I TCNGA 2 cut(s) 14, 228
Hpy188III TCNNGA 3 cut(s) 76, 293, 319
HpyAV CCTTC 2 cut(s) 170, 347
HpyCH4III ACNGT 2 cut(s) 327, 349
HpyCH4V TGCA 3 cut(s) 138, 193, 360
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 2 cut(s) 138, 392
HspAI GCGC 1 cut(s) 50
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 34, 287
LweI GCATC 1 cut(s) 219
MabI ACCWGGT 1 cut(s) 109
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 182
MboII GAAGA 2 cut(s) 40, 294
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 1 cut(s) 268
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 7 cut(s) 8, 11, 26, 264, 267, 270, 318
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 2 cut(s) 57, 328
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 3 cut(s) 25, 41, 159
MspR9I CCNGG 3 cut(s) 111, 160, 204
MvaI CCWGG 2 cut(s) 111, 204
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 388
NlaIII CATG 2 cut(s) 138, 392
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 182
NsbI TGCGCA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 328
PkrI GCNGC 3 cut(s) 49, 71, 277
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Psp6I CCWGG 2 cut(s) 109, 202
PspGI CCWGG 2 cut(s) 109, 202
PspN4I GGNNCC 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 2 cut(s) 57, 328
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 3 cut(s) 48, 70, 276
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 3 cut(s) 111, 160, 204
SduI GDGCHC 1 cut(s) 167
SetI ASST 6 cut(s) 22, 49, 112, 316, 358, 406
SexAI ACCWGGT 1 cut(s) 109
SfaNI GCATC 1 cut(s) 219
SmiMI CAYNNNNRTG 2 cut(s) 57, 328
Sse9I AATT 1 cut(s) 268
SsiI CCGC 2 cut(s) 69, 281
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 109, 158, 202
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 327, 349
TaqI TCGA 1 cut(s) 128
TasI AATT 1 cut(s) 268
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 72
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 2 cut(s) 47, 275
Tsp45I GTSAC 1 cut(s) 182
TspRI CASTG 1 cut(s) 225
XagI CCTNNNNNAGG 1 cut(s) 114
XapI RAATTY 1 cut(s) 268
XcmI CCANNNNNNNNNTGG 3 cut(s) 91, 386, 395
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.