MD11G1017100.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001285
Physical Location & Seq
Reverse (-)
1384760 .. 1387902
3143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1017100.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGAGTTCGGAGGAGGTGGCCGGAAGAGAGGCAGGCCGGAAGCTGGGCACTTTAATGGCAATGGCGGCTTCAAGAAATCCAAGCCAGAAATGGAGTCCTTTCAACCTGGTATAGGAAGCAAATCGAAGCCATGCACAAAGTTTTTCAGCACTTCTGGGTGCCCTTTTGGTGAGGGATGTCATTTCTTGCATTATGTTCCTGGTGGCATCAAAGCGGTGTCTCAGATGACTGGTAGCAACCCAACTCATCCTCCTCCTCCTAGAAATTCAGCAGCCCCACCATCATTTCCAGATGGTTCATCTCCTCCAACTGTCAAGACACGGTTGTGTAACAAGTACAACACAGTTGAAGGTTGCAAGTTCGGGGATAAATGCCATTTTGCACATGGTGACTGGGAGCTTGGTAGGCCCACTGCTCAATCCTATGAAGATCCTCGTACAGGGGGAGGATTTCCGGGCAGGATGGGAGGTCGGATGGAGCCTCCCCCGCCAGCACATGGAGCTGTAGCAAGTTTTGGGGCCACAGCCACAGCTAAGATCAGTGTTGACGCTTCACTTGCTGGAGCCATAATTGGGAAAAACGGTGTGCACTCAAAGCAAATTTGTCGTGTGACTGGAGCGAAGCTTTCTATAAGAGAGCATGAAACAGATCATAAGCTGAGGAACATTGAGCTTGAGGGAACCTTGGACCAGATTAAAGACGCAAGTGCCATGGTTCGTGAGCTTATTGGGAACGTGAGTTCAGGTGCTGGAGCTCACAACATGAGGAACCCTGCTATATCAGCCTCATCTGCGGCAAGCAACTTCAAGACTAAGCTTTGTGAGAACTTTACTAAAGGTACATGCACCTTTGGGGATAGGTGCCACTTTGCACATGGACAAGAAGAGTTGCGCAGGTCGGGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 zf-CCCH zf-CCCH_2

Protein Analysis

303

Amino Acids

31.96

Weight (kDa)

9.32

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 177 - 241 2e-11 KH domain
zf-CCCH PF00642 269 - 294 5.4e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH_4 PF18044 272 - 292 8.5e-06 CCCH-type zinc finger
zf_CCCH_4 PF18345 274 - 292 6.8e-06 Zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 885
AccB1I GGYRCC 2 cut(s) 159, 861
AccB7I CCANNNNNTGG 1 cut(s) 497
AciI CCGC 4 cut(s) 66, 215, 488, 794
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 2 cut(s) 265, 600
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 3 cut(s) 338, 439, 841
AfiI CCNNNNNNNGG 5 cut(s) 44, 113, 440, 497, 573
AgsI TTSAA 4 cut(s) 73, 104, 350, 808
AjnI CCWGG 2 cut(s) 106, 199
AleI CACNNNNGTG 1 cut(s) 325
AloI GAACNNNNNNTCC 2 cut(s) 724, 756
Alw21I GWGCWC 2 cut(s) 591, 757
Alw26I GTCTC 1 cut(s) 225
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 4 cut(s) 19, 35, 407, 519
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 2 cut(s) 265, 600
AspLEI GCGC 1 cut(s) 894
AspS9I GGNCC 3 cut(s) 408, 519, 688
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 182, 401
AvaII GGWCC 1 cut(s) 688
BaeGI GKGCMC 3 cut(s) 51, 164, 591
BanI GGYRCC 2 cut(s) 159, 861
BanII GRGCYC 1 cut(s) 757
Bbv12I GWGCWC 2 cut(s) 591, 757
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 1 cut(s) 284
BccI CCATC 4 cut(s) 287, 289, 457, 469
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 2 cut(s) 108, 201
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 885
BisI GCNGC 3 cut(s) 67, 273, 795
BlsI GCNGC 3 cut(s) 68, 274, 796
Bme1390I CCNGG 3 cut(s) 108, 201, 456
Bme18I GGWCC 1 cut(s) 688
BmgT120I GGNCC 3 cut(s) 408, 519, 688
BmiI GGNNCC 7 cut(s) 161, 480, 520, 565, 682, 770, 863
BmrFI CCNGG 3 cut(s) 108, 201, 456
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 403
BpmI CTGGAG 3 cut(s) 582, 636, 771
Bpu10I CCTNAGC 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 684, 711
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 113, 440, 497, 573
Bse1I ACTGG 3 cut(s) 235, 398, 619
Bse3DI GCAATG 1 cut(s) 67
BseBI CCWGG 2 cut(s) 108, 201
BseDI CCNNGG 2 cut(s) 684, 711
BseGI GGATG 5 cut(s) 182, 247, 468, 480, 909
BseLI CCNNNNNNNGG 5 cut(s) 44, 113, 440, 497, 573
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 236, 650
BseNI ACTGG 3 cut(s) 235, 398, 619
BseRI GAGGAG 4 cut(s) 27, 243, 246, 294
BseSI GKGCMC 3 cut(s) 51, 164, 591
BseXI GCAGC 1 cut(s) 284
BseYI CCCAGC 1 cut(s) 44
BshFI GGCC 4 cut(s) 21, 37, 409, 521
BshNI GGYRCC 2 cut(s) 159, 861
BsiHKAI GWGCWC 2 cut(s) 591, 757
BsiSI CCGG 3 cut(s) 22, 38, 455
BslI CCNNNNNNNGG 5 cut(s) 44, 113, 440, 497, 573
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 4 cut(s) 21, 37, 409, 521
Bsp1286I GDGCHC 4 cut(s) 51, 164, 591, 757
Bsp143I GATC 3 cut(s) 430, 537, 649
Bsp19I CCATGG 1 cut(s) 711
BspACI CCGC 4 cut(s) 66, 215, 488, 794
BspANI GGCC 4 cut(s) 21, 37, 409, 521
BspCNI CTCAG 2 cut(s) 235, 651
BspLI GGNNCC 7 cut(s) 161, 480, 520, 565, 682, 770, 863
BspMI ACCTGC 1 cut(s) 885
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 2 cut(s) 159, 861
BsrDI GCAATG 1 cut(s) 67
BsrI ACTGG 3 cut(s) 235, 398, 619
BssECI CCNNGG 2 cut(s) 684, 711
BssMI GATC 3 cut(s) 430, 537, 649
BssT1I CCWWGG 2 cut(s) 684, 711
Bst2UI CCWGG 2 cut(s) 108, 201
Bst4CI ACNGT 4 cut(s) 313, 324, 346, 584
Bst6I CTCTTC 2 cut(s) 20, 879
BstC8I GCNNGC 3 cut(s) 35, 492, 799
BstDEI CTNAG 4 cut(s) 222, 534, 659, 813
BstDSI CCRYGG 1 cut(s) 711
BstENI CCTNNNNNAGG 2 cut(s) 111, 438
BstF5I GGATG 5 cut(s) 182, 247, 468, 480, 909
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 3 cut(s) 433, 540, 652
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 3 cut(s) 430, 537, 649
BstMWI GCNNNNNNNGC 8 cut(s) 66, 406, 487, 500, 557, 595, 782, 791
BstNI CCWGG 2 cut(s) 108, 201
BstNSI RCATGY 1 cut(s) 846
BstSCI CCNGG 3 cut(s) 106, 199, 454
BstSFI CTRYAG 1 cut(s) 504
BstSLI GKGCMC 3 cut(s) 51, 164, 591
BstV1I GCAGC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BsuRI GGCC 4 cut(s) 21, 37, 409, 521
BtgI CCRYGG 1 cut(s) 711
BtsCI GGATG 5 cut(s) 182, 247, 468, 480, 909
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 411, 547
BveI ACCTGC 1 cut(s) 885
Cac8I GCNNGC 3 cut(s) 35, 492, 799
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 1 cut(s) 894
Cfr13I GGNCC 3 cut(s) 408, 519, 688
CseI GACGC 2 cut(s) 557, 710
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 3 cut(s) 337, 438, 840
CviAII CATG 8 cut(s) 132, 386, 497, 641, 712, 763, 843, 875
CviQI GTAC 3 cut(s) 337, 438, 840
DdeI CTNAG 4 cut(s) 222, 534, 659, 813
DpnI GATC 3 cut(s) 432, 539, 651
DpnII GATC 3 cut(s) 430, 537, 649
DraIII CACNNNGTG 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 20, 879
EarI CTCTTC 2 cut(s) 20, 879
Ecl136II GAGCTC 1 cut(s) 755
Eco130I CCWWGG 2 cut(s) 684, 711
Eco24I GRGCYC 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 755
EcoNI CCTNNNNNAGG 2 cut(s) 111, 438
EcoRII CCWGG 2 cut(s) 106, 199
EcoT14I CCWWGG 2 cut(s) 684, 711
EcoT38I GRGCYC 1 cut(s) 757
ErhI CCWWGG 2 cut(s) 684, 711
FaeI CATG 8 cut(s) 135, 389, 500, 644, 715, 766, 846, 878
FatI CATG 8 cut(s) 131, 385, 496, 640, 711, 762, 842, 874
FauI CCCGC 1 cut(s) 495
Fnu4HI GCNGC 3 cut(s) 67, 273, 795
FokI GGATG 4 cut(s) 189, 234, 475, 487
FriOI GRGCYC 1 cut(s) 757
Fsp4HI GCNGC 3 cut(s) 67, 273, 795
FspBI CTAG 1 cut(s) 261
FspI TGCGCA 1 cut(s) 893
GlaI GCGC 1 cut(s) 893
GluI GCNGC 3 cut(s) 67, 273, 795
GsaI CCCAGC 1 cut(s) 48
GsuI CTGGAG 3 cut(s) 582, 636, 771
HaeIII GGCC 4 cut(s) 21, 37, 409, 521
HapII CCGG 3 cut(s) 22, 38, 455
HgaI GACGC 2 cut(s) 557, 710
HhaI GCGC 1 cut(s) 894
Hin1II CATG 8 cut(s) 135, 389, 500, 644, 715, 766, 846, 878
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 2 cut(s) 623, 815
HinfI GANTC 1 cut(s) 95
HpaII CCGG 3 cut(s) 22, 38, 455
HphI GGTGA 2 cut(s) 182, 401
Hpy166II GTNNAC 2 cut(s) 547, 589
Hpy188I TCNGA 3 cut(s) 11, 225, 474
Hpy188III TCNNGA 5 cut(s) 73, 290, 316, 719, 808
Hpy8I GTNNAC 2 cut(s) 547, 589
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 4 cut(s) 313, 324, 346, 584
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 7 cut(s) 135, 190, 357, 383, 589, 846, 872
HpyF10VI GCNNNNNNNGC 8 cut(s) 66, 406, 487, 500, 557, 595, 782, 791
HpyF3I CTNAG 4 cut(s) 222, 534, 659, 813
HpySE526I ACGT 1 cut(s) 735
Hsp92II CATG 8 cut(s) 135, 389, 500, 644, 715, 766, 846, 878
HspAI GCGC 1 cut(s) 892
Kzo9I GATC 3 cut(s) 430, 537, 649
LmnI GCTCC 6 cut(s) 397, 478, 500, 563, 617, 752
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 1 cut(s) 216
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 3 cut(s) 329, 389, 610
MalI GATC 3 cut(s) 432, 539, 651
MboI GATC 3 cut(s) 430, 537, 649
MboII GAAGA 3 cut(s) 37, 440, 896
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 4 cut(s) 51, 164, 591, 757
MluCI AATT 3 cut(s) 265, 570, 600
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 332, 452
MseI TTAA 2 cut(s) 54, 696
MslI CAYNNNNRTG 2 cut(s) 54, 325
MspI CCGG 3 cut(s) 22, 38, 455
MspR9I CCNGG 3 cut(s) 108, 201, 456
MvaI CCWGG 2 cut(s) 108, 201
MwoI GCNNNNNNNGC 8 cut(s) 66, 406, 487, 500, 557, 595, 782, 791
NciI CCSGG 1 cut(s) 456
NcoI CCATGG 1 cut(s) 711
NdeII GATC 3 cut(s) 430, 537, 649
NlaIII CATG 8 cut(s) 135, 389, 500, 644, 715, 766, 846, 878
NlaIV GGNNCC 7 cut(s) 161, 480, 520, 565, 682, 770, 863
NmuCI GTSAC 2 cut(s) 389, 610
NsbI TGCGCA 1 cut(s) 893
NspI RCATGY 1 cut(s) 846
OliI CACNNNNGTG 1 cut(s) 325
PflMI CCANNNNNTGG 1 cut(s) 497
PkrI GCNGC 3 cut(s) 68, 274, 796
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 757
Psp6I CCWGG 2 cut(s) 106, 199
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 2 cut(s) 106, 199
PspN4I GGNNCC 7 cut(s) 161, 480, 520, 565, 682, 770, 863
PspPI GGNCC 3 cut(s) 408, 519, 688
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 430
RsaI GTAC 3 cut(s) 338, 439, 841
RsaNI GTAC 3 cut(s) 337, 438, 840
RseI CAYNNNNRTG 2 cut(s) 54, 325
SacI GAGCTC 1 cut(s) 757
SaqAI TTAA 2 cut(s) 54, 696
SatI GCNGC 3 cut(s) 67, 273, 795
Sau3AI GATC 3 cut(s) 430, 537, 649
Sau96I GGNCC 3 cut(s) 408, 519, 688
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 108, 201, 456
SduI GDGCHC 4 cut(s) 51, 164, 591, 757
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 1 cut(s) 504
SinI GGWCC 1 cut(s) 688
SmiMI CAYNNNNRTG 2 cut(s) 54, 325
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 3 cut(s) 265, 570, 600
SsiI CCGC 4 cut(s) 66, 215, 488, 794
SspMI CTAG 1 cut(s) 261
SstI GAGCTC 1 cut(s) 757
StyD4I CCNGG 3 cut(s) 106, 199, 454
StyI CCWWGG 2 cut(s) 684, 711
TaaI ACNGT 4 cut(s) 313, 324, 346, 584
TaiI ACGT 1 cut(s) 738
TaqI TCGA 1 cut(s) 125
TasI AATT 3 cut(s) 265, 570, 600
TatI WGTACW 1 cut(s) 336
TauI GCSGC 2 cut(s) 69, 797
Tru1I TTAA 2 cut(s) 54, 696
Tru9I TTAA 2 cut(s) 54, 696
TscAI CASTG 2 cut(s) 418, 547
TseFI GTSAC 2 cut(s) 389, 610
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 2 cut(s) 389, 610
TspDTI ATGAA 3 cut(s) 288, 441, 657
TspRI CASTG 2 cut(s) 418, 547
Van91I CCANNNNNTGG 1 cut(s) 497
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 688
XagI CCTNNNNNAGG 2 cut(s) 111, 438
XapI RAATTY 2 cut(s) 265, 600
XceI RCATGY 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 3 cut(s) 88, 383, 872
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.