MD12G1059600.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001285
Physical Location & Seq
Forward (+)
6930882 .. 6934318
3437 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1059600.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGATACTCGTAAAAGAGGTCGACATGAAGGTGGCGGCTTTCACTCTAATGGCGGCTTCAAGAAGAACAAGCAAGAATTGGAGTCCTTTTCAACTGGTGTAGGAAGCAAATCGAAGCCATGCACCAAATTTTTCAGCACTGCTGGATGTCCTTTTGGTGAGAGTTGCCATTTTCTGCACTACGTTCCTGGAGGTTACAATGCCGTAGCTCAAATGATGAATCTGGCGCCTGCTCCTCCACCACCTAGGAACATTCCTGGCCCACCATCCCATTCCAATGGATCCAATTCATCTGGAGTTAAATCACGCATATGTACTAAATATAATTCTCCTGAAGGCTGCAAATTTGGTGACAAATGTCATTTTGCTCATGGTGAGTGGGAACTCGGCAAGCCTCTTGCTCAGTCCCACGATGATCCTCGTGCCATGGGACCTGGTCCAGGCCGTATGGGTAATTGGATGGAGCCACACCTGGCAGGCCCTGCATCTAGCTTTGGTGCCTCATCTACAGCAAAAATCAGTGTGGATGCTTCACATGCAGGAGCCATAATTGGGAAGGGTGGTGTGCATTCGAAGCAGATTTGTCTCCGGACAGGAGCCAAGCTTTCAATCCGGGACCATGAGTCAAATCCCAATCTCAGGAACATTGAACTTGAGGGGACCTTTGAACAGATTCAGCAAGCTAGTGCCATGGTAAGCGAGCTAATTGCGACTGTTTCAACCTCTGGCCCTGGAAAGGCTCCAGGAGGAGGAGGAGGTGGAGGAGGAGGCCGAGGAGCCCCAGCCCCTCCCAGAAGTAACTATAAGACGAAACTATGTGATAATTTTACCAAGGGGACTTGCACCTTTGGAGAAAGATGTCACTTTGCACACGGGGCTGCTGAATTGCGGAAGTCTGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 zf-CCCH zf-CCCH_2

Protein Analysis

302

Amino Acids

31.4

Weight (kDa)

9.39

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 170 - 233 1e-12 KH domain
zf-CCCH PF00642 268 - 292 7.7e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 226, 497
AccI GTMKAC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 588
AciI CCGC 3 cut(s) 36, 54, 889
AclWI GGATC 3 cut(s) 276, 289, 410
AcsI RAATTY 2 cut(s) 128, 344
AcuI CTGAAG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 227
AfaI GTAC 1 cut(s) 316
AfiI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
AgsI TTSAA 6 cut(s) 61, 93, 609, 650, 668, 720
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 7 cut(s) 187, 256, 433, 439, 471, 730, 742
AluBI AGCT 5 cut(s) 209, 492, 604, 683, 703
AluI AGCT 5 cut(s) 209, 492, 604, 683, 703
Alw26I GTCTC 1 cut(s) 590
AlwI GGATC 3 cut(s) 276, 289, 410
AlwNI CAGNNNCTG 1 cut(s) 482
Aor13HI TCCGGA 1 cut(s) 588
AoxI GGCC 5 cut(s) 259, 442, 478, 727, 769
ApeKI GCWGC 2 cut(s) 339, 878
ApoI RAATTY 2 cut(s) 128, 344
Asp700I GAANNNNTTC 1 cut(s) 672
AspA2I CCTAGG 1 cut(s) 245
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 7 cut(s) 260, 431, 437, 479, 616, 660, 728
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 3 cut(s) 170, 362, 386
AsuII TTCGAA 1 cut(s) 572
AvaII GGWCC 4 cut(s) 431, 437, 616, 660
AvrII CCTAGG 1 cut(s) 245
BamHI GGATCC 1 cut(s) 281
BanI GGYRCC 2 cut(s) 226, 497
BanII GRGCYC 1 cut(s) 781
BauI CACGAG 1 cut(s) 420
BbvI GCAGC 2 cut(s) 326, 865
BccI CCATC 2 cut(s) 274, 454
BceAI ACGGC 2 cut(s) 188, 429
BcgI CGANNNNNNTGC 2 cut(s) 689, 723
BciT130I CCWGG 7 cut(s) 189, 258, 435, 441, 473, 732, 744
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 3 cut(s) 246, 489, 684
BfmI CTRYAG 1 cut(s) 507
BfoI RGCGCY 1 cut(s) 230
BisI GCNGC 4 cut(s) 37, 55, 340, 879
BlnI CCTAGG 1 cut(s) 245
BlsI GCNGC 4 cut(s) 38, 56, 341, 880
Bme1390I CCNGG 8 cut(s) 189, 258, 435, 441, 473, 614, 732, 744
Bme18I GGWCC 4 cut(s) 431, 437, 616, 660
BmeRI GACNNNNNGTC 1 cut(s) 622
BmgT120I GGNCC 7 cut(s) 260, 431, 437, 479, 616, 660, 728
BmrFI CCNGG 8 cut(s) 189, 258, 435, 441, 473, 614, 732, 744
BmsI GCATC 2 cut(s) 494, 517
BoxI GACNNNNGTC 1 cut(s) 357
BpmI CTGGAG 3 cut(s) 210, 315, 726
Bpu14I TTCGAA 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 6 cut(s) 245, 426, 690, 730, 772, 831
BsaWI WCCGGW 1 cut(s) 588
Bsc4I CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
Bse1I ACTGG 1 cut(s) 100
BseAI TCCGGA 1 cut(s) 588
BseBI CCWGG 7 cut(s) 189, 258, 435, 441, 473, 732, 744
BseDI CCNNGG 6 cut(s) 245, 426, 690, 730, 772, 831
BseGI GGATG 4 cut(s) 152, 266, 465, 532
BseLI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
BseMII CTCAG 2 cut(s) 416, 652
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 7 cut(s) 225, 762, 765, 768, 777, 780, 789
BseXI GCAGC 2 cut(s) 326, 865
BseYI CCCAGC 1 cut(s) 781
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 5 cut(s) 261, 444, 480, 729, 771
BshNI GGYRCC 2 cut(s) 226, 497
BsiSI CCGG 2 cut(s) 589, 613
BslFI GGGAC 5 cut(s) 391, 444, 629, 673, 850
BslI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 5 cut(s) 391, 444, 629, 673, 850
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 5 cut(s) 261, 444, 480, 729, 771
Bsp119I TTCGAA 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 781
Bsp13I TCCGGA 1 cut(s) 588
Bsp143I GATC 2 cut(s) 281, 415
Bsp19I CCATGG 2 cut(s) 426, 690
BspACI CCGC 3 cut(s) 36, 54, 889
BspANI GGCC 5 cut(s) 261, 444, 480, 729, 771
BspCNI CTCAG 2 cut(s) 415, 651
BspEI TCCGGA 1 cut(s) 588
BspPI GGATC 3 cut(s) 276, 289, 410
BspT104I TTCGAA 1 cut(s) 572
BspT107I GGYRCC 2 cut(s) 226, 497
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 6 cut(s) 245, 426, 690, 730, 772, 831
BssMI GATC 2 cut(s) 281, 415
BssNI GRCGYC 1 cut(s) 227
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 4 cut(s) 245, 426, 690, 831
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 7 cut(s) 189, 258, 435, 441, 473, 732, 744
Bst4CI ACNGT 1 cut(s) 715
BstACI GRCGYC 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBI TTCGAA 1 cut(s) 572
BstC8I GCNNGC 5 cut(s) 231, 392, 478, 681, 701
BstDEI CTNAG 2 cut(s) 402, 638
BstDSI CCRYGG 2 cut(s) 426, 690
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 4 cut(s) 152, 266, 465, 532
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 2 cut(s) 284, 418
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 2 cut(s) 281, 415
BstMWI GCNNNNNNNGC 4 cut(s) 482, 536, 574, 875
BstNI CCWGG 7 cut(s) 189, 258, 435, 441, 473, 732, 744
BstNSI RCATGY 1 cut(s) 539
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 8 cut(s) 187, 256, 433, 439, 471, 612, 730, 742
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 326, 865
BstX2I RGATCY 1 cut(s) 281
BstXI CCANNNNNNTGG 1 cut(s) 278
BstYI RGATCY 1 cut(s) 281
BsuRI GGCC 5 cut(s) 261, 444, 480, 729, 771
BtgI CCRYGG 2 cut(s) 426, 690
BtsCI GGATG 4 cut(s) 152, 266, 465, 532
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 138, 526
Cac8I GCNNGC 5 cut(s) 231, 392, 478, 681, 701
CaiI CAGNNNCTG 1 cut(s) 482
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 7 cut(s) 260, 431, 437, 479, 616, 660, 728
CsiI ACCWGGT 1 cut(s) 433
Csp6I GTAC 1 cut(s) 315
CviAII CATG 7 cut(s) 26, 120, 371, 427, 536, 620, 691
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 2 cut(s) 402, 638
DinI GGCGCC 1 cut(s) 228
DpnI GATC 2 cut(s) 283, 417
DpnII GATC 2 cut(s) 281, 415
DriI GACNNNNNGTC 1 cut(s) 622
Eam1105I GACNNNNNGTC 1 cut(s) 622
Eco130I CCWWGG 4 cut(s) 245, 426, 690, 831
Eco24I GRGCYC 1 cut(s) 781
Eco47I GGWCC 4 cut(s) 431, 437, 616, 660
Eco57I CTGAAG 1 cut(s) 354
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoO109I RGGNCCY 3 cut(s) 431, 479, 660
EcoRII CCWGG 7 cut(s) 187, 256, 433, 439, 471, 730, 742
EcoT14I CCWWGG 4 cut(s) 245, 426, 690, 831
EcoT38I GRGCYC 1 cut(s) 781
EgeI GGCGCC 1 cut(s) 228
EheI GGCGCC 1 cut(s) 228
ErhI CCWWGG 4 cut(s) 245, 426, 690, 831
FaeI CATG 7 cut(s) 29, 123, 374, 430, 539, 623, 694
FaqI GGGAC 5 cut(s) 391, 444, 629, 673, 850
FatI CATG 7 cut(s) 25, 119, 370, 426, 535, 619, 690
FauNDI CATATG 1 cut(s) 311
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 4 cut(s) 37, 55, 340, 879
FokI GGATG 4 cut(s) 159, 253, 472, 539
FriOI GRGCYC 1 cut(s) 781
Fsp4HI GCNGC 4 cut(s) 37, 55, 340, 879
FspBI CTAG 3 cut(s) 246, 489, 684
GlaI GCGC 1 cut(s) 228
GluI GCNGC 4 cut(s) 37, 55, 340, 879
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 3 cut(s) 210, 315, 726
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 5 cut(s) 261, 444, 480, 729, 771
HapII CCGG 2 cut(s) 589, 613
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 7 cut(s) 29, 123, 374, 430, 539, 623, 694
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 23
HindII GTYRAC 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 4 cut(s) 83, 220, 623, 673
HpaII CCGG 2 cut(s) 589, 613
HphI GGTGA 3 cut(s) 170, 362, 386
Hpy166II GTNNAC 1 cut(s) 23
Hpy188III TCNNGA 6 cut(s) 61, 294, 332, 589, 640, 897
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 23, 329, 550
HpyCH4III ACNGT 1 cut(s) 715
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 8 cut(s) 123, 178, 342, 485, 539, 568, 843, 869
HpyF10VI GCNNNNNNNGC 4 cut(s) 482, 536, 574, 875
HpyF3I CTNAG 2 cut(s) 402, 638
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 7 cut(s) 29, 123, 374, 430, 539, 623, 694
HspAI GCGC 1 cut(s) 227
KasI GGCGCC 1 cut(s) 226
Kpn2I TCCGGA 1 cut(s) 588
Kzo9I GATC 2 cut(s) 281, 415
LmnI GCTCC 6 cut(s) 238, 463, 542, 596, 745, 776
Lsp1109I GCAGC 2 cut(s) 326, 865
LweI GCATC 2 cut(s) 494, 517
MabI ACCWGGT 1 cut(s) 433
MaeI CTAG 3 cut(s) 246, 489, 684
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 4 cut(s) 194, 350, 797, 860
MalI GATC 2 cut(s) 283, 417
MboI GATC 2 cut(s) 281, 415
MboII GAAGA 1 cut(s) 76
MflI RGATCY 1 cut(s) 281
MhlI GDGCHC 1 cut(s) 781
Mly113I GGCGCC 1 cut(s) 227
MlyI GAGTC 2 cut(s) 92, 632
MroI TCCGGA 1 cut(s) 588
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 4 cut(s) 30, 48, 276, 310
MspI CCGG 2 cut(s) 589, 613
MspR9I CCNGG 8 cut(s) 189, 258, 435, 441, 473, 614, 732, 744
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 7 cut(s) 189, 258, 435, 441, 473, 732, 744
MwoI GCNNNNNNNGC 4 cut(s) 482, 536, 574, 875
NarI GGCGCC 1 cut(s) 227
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 2 cut(s) 426, 690
NdeI CATATG 1 cut(s) 311
NdeII GATC 2 cut(s) 281, 415
NlaIII CATG 7 cut(s) 29, 123, 374, 430, 539, 623, 694
NmeAIII GCCGAG 2 cut(s) 366, 797
NmuCI GTSAC 2 cut(s) 350, 860
NspI RCATGY 1 cut(s) 539
NspV TTCGAA 1 cut(s) 572
PctI GAATGC 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 220, 673
PflFI GACNNNGTC 1 cut(s) 435
PfoI TCCNGGA 3 cut(s) 187, 612, 742
PkrI GCNGC 4 cut(s) 38, 56, 341, 880
PleI GAGTC 2 cut(s) 91, 631
PluTI GGCGCC 1 cut(s) 230
PpsI GAGTC 2 cut(s) 91, 631
PpuMI RGGWCCY 2 cut(s) 431, 660
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 2 cut(s) 431, 660
Psp6I CCWGG 7 cut(s) 187, 256, 433, 439, 471, 730, 742
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 7 cut(s) 187, 256, 433, 439, 471, 730, 742
PspPI GGNCC 7 cut(s) 260, 431, 437, 479, 616, 660, 728
PspPPI RGGWCCY 2 cut(s) 431, 660
PstNI CAGNNNCTG 1 cut(s) 482
PsuI RGATCY 1 cut(s) 281
PsyI GACNNNGTC 1 cut(s) 435
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 4 cut(s) 30, 48, 276, 310
SalI GTCGAC 1 cut(s) 21
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 4 cut(s) 37, 55, 340, 879
Sau3AI GATC 2 cut(s) 281, 415
Sau96I GGNCC 7 cut(s) 260, 431, 437, 479, 616, 660, 728
SchI GAGTC 2 cut(s) 92, 632
ScrFI CCNGG 8 cut(s) 189, 258, 435, 441, 473, 614, 732, 744
SduI GDGCHC 1 cut(s) 781
SexAI ACCWGGT 1 cut(s) 433
SfaNI GCATC 2 cut(s) 494, 517
SfcI CTRYAG 1 cut(s) 507
SfoI GGCGCC 1 cut(s) 228
SfuI TTCGAA 1 cut(s) 572
SinI GGWCC 4 cut(s) 431, 437, 616, 660
SmiMI CAYNNNNRTG 4 cut(s) 30, 48, 276, 310
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SsiI CCGC 3 cut(s) 36, 54, 889
SspDI GGCGCC 1 cut(s) 226
SspMI CTAG 3 cut(s) 246, 489, 684
StyD4I CCNGG 8 cut(s) 187, 256, 433, 439, 471, 612, 730, 742
StyI CCWWGG 4 cut(s) 245, 426, 690, 831
TaaI ACNGT 1 cut(s) 715
TaiI ACGT 1 cut(s) 186
TaqI TCGA 3 cut(s) 22, 113, 572
TatI WGTACW 1 cut(s) 314
TauI GCSGC 2 cut(s) 39, 57
TfiI GAWTC 2 cut(s) 220, 673
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 145, 526
TseFI GTSAC 2 cut(s) 350, 860
TseI GCWGC 2 cut(s) 339, 878
Tsp45I GTSAC 2 cut(s) 350, 860
TspDTI ATGAA 3 cut(s) 42, 233, 279
TspRI CASTG 2 cut(s) 145, 526
Tth111I GACNNNGTC 1 cut(s) 435
VpaK11BI GGWCC 4 cut(s) 431, 437, 616, 660
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 128, 344
XceI RCATGY 1 cut(s) 539
XmaJI CCTAGG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 3 cut(s) 246, 489, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.