MD14G1058500.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001285
Physical Location & Seq
Reverse (-)
6053219 .. 6056632
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1058500.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGATACTCGTAAAAGAGGGCGGCATGAAGGTGGCGGCTTTCACTCTAATGGCGGCTTCAAGAAGAACAAGCAAGAATTGGAGTCCTTTTCAACTGGTGTAGGAAGCAAATCGAAGCCATGCACCAAATTTTTCAGCACTGCTGGCTGTCCCTTTGGCGAGAGTTGCCACTTCCTGCACTATGTTCCTGGAGGTTACAATGCCGTGGCTCAGATGATGAATCTTGCGCCTGCCCCTCCGCCACCTAGGAACATTTCTGGCCCACCATCCAATTCCAATGGGTCCAATTCATCTGGAGTTAAATCGCGCATATGCAATAAATATAATTCTGCTGAAGGCTGCAAATTTGGTGACAAATGTCATTTTGCTCATGGTGAGTGGGAACTTGGCAAGCCTCTTGCTCAGACCCACGATGATCCTCGTGCCATGGGACCTGGTCCAGGCCGTATGGGTAATTGGATGGAGCCACACGCGGCACGCCCTGCATCCAGCTTTGGTGCCTCATCTACTGCAAAAATCAGTGTGGATGCATCACTTGCAGGAGCCATTATTGGGAAGGGAGGTGTACATTCAAAGCAGATTTGTCGTCAGACGGGAGCCAAGCTTTCAATCCGGGATCATGAGTCAAATCCCAATCTCAGGAACATTGAACTGGAGGGGACCTTTGAACAGATTCAGCAAGCTAGTGCCATGGTCAGCGAGCTAATTGCGACTGTTTCAATCTCCGGCCCTGGTAAGGCTCCAGGAGGAGGAGGAGGAGGCCCAGGAGCCCCAGCCCCTCCAGGAAGTAACTATAAGACGAAACTATGTGATAATTTTACCAAGGGGACTTGCACCTTTGGAGAAAGATGTCACTTTGCACACGGGGCTGCTGAATTGCGGAAGTCTGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 zf-CCCH zf-CCCH_2

Protein Analysis

299

Amino Acids

31.1

Weight (kDa)

9.31

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 170 - 233 1.2e-13 KH domain
zf-CCCH PF00642 265 - 289 7.6e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 497
AccII CGCG 2 cut(s) 307, 473
AciI CCGC 6 cut(s) 22, 36, 54, 239, 473, 880
AclWI GGATC 2 cut(s) 410, 624
AcsI RAATTY 2 cut(s) 128, 344
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
AgsI TTSAA 7 cut(s) 61, 93, 573, 609, 650, 668, 720
AjnI CCWGG 7 cut(s) 187, 433, 439, 730, 742, 763, 781
AluBI AGCT 4 cut(s) 492, 604, 683, 703
AluI AGCT 4 cut(s) 492, 604, 683, 703
AlwI GGATC 2 cut(s) 410, 624
AoxI GGCC 4 cut(s) 259, 442, 727, 760
ApeKI GCWGC 2 cut(s) 339, 869
ApoI RAATTY 2 cut(s) 128, 344
Asp700I GAANNNNTTC 1 cut(s) 672
AspA2I CCTAGG 1 cut(s) 245
AspLEI GCGC 2 cut(s) 229, 309
AspS9I GGNCC 7 cut(s) 260, 282, 431, 437, 660, 728, 761
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 362, 386
AvaII GGWCC 4 cut(s) 282, 431, 437, 660
AvrII CCTAGG 1 cut(s) 245
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 772
BauI CACGAG 1 cut(s) 420
BbvI GCAGC 2 cut(s) 326, 856
BccI CCATC 2 cut(s) 274, 454
BceAI ACGGC 2 cut(s) 188, 429
BcgI CGANNNNNNTGC 4 cut(s) 566, 600, 689, 723
BciT130I CCWGG 7 cut(s) 189, 435, 441, 732, 744, 765, 783
BcnI CCSGG 1 cut(s) 614
BfaI CTAG 2 cut(s) 246, 684
BisI GCNGC 6 cut(s) 23, 37, 55, 340, 474, 870
BlnI CCTAGG 1 cut(s) 245
BlsI GCNGC 6 cut(s) 24, 38, 56, 341, 475, 871
Bme1390I CCNGG 8 cut(s) 189, 435, 441, 614, 732, 744, 765, 783
Bme18I GGWCC 4 cut(s) 282, 431, 437, 660
BmgT120I GGNCC 7 cut(s) 260, 282, 431, 437, 660, 728, 761
BmiI GGNNCC 9 cut(s) 283, 432, 465, 499, 544, 598, 661, 741, 769
BmrFI CCNGG 8 cut(s) 189, 435, 441, 614, 732, 744, 765, 783
BmsI GCATC 3 cut(s) 494, 517, 539
BoxI GACNNNNGTC 1 cut(s) 357
BpmI CTGGAG 5 cut(s) 210, 315, 674, 726, 765
BpuMI CCSGG 1 cut(s) 614
BsaJI CCNNGG 7 cut(s) 204, 245, 426, 690, 730, 763, 822
Bsc4I CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
Bse1I ACTGG 2 cut(s) 100, 657
BseBI CCWGG 7 cut(s) 189, 435, 441, 732, 744, 765, 783
BseDI CCNNGG 7 cut(s) 204, 245, 426, 690, 730, 763, 822
BseGI GGATG 4 cut(s) 266, 465, 485, 532
BseLI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
BseMII CTCAG 3 cut(s) 224, 416, 652
BseNI ACTGG 2 cut(s) 100, 657
BseRI GAGGAG 4 cut(s) 762, 765, 768, 771
BseXI GCAGC 2 cut(s) 326, 856
BseYI CCCAGC 1 cut(s) 772
BsgI GTGCAG 1 cut(s) 161
Bsh1236I CGCG 2 cut(s) 307, 473
BshFI GGCC 4 cut(s) 261, 444, 729, 762
BshNI GGYRCC 1 cut(s) 497
BsiSI CCGG 2 cut(s) 613, 726
BslFI GGGAC 4 cut(s) 135, 444, 673, 841
BslI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
BsmFI GGGAC 4 cut(s) 135, 444, 673, 841
BsnI GGCC 4 cut(s) 261, 444, 729, 762
Bsp1286I GDGCHC 1 cut(s) 772
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 2 cut(s) 415, 616
Bsp19I CCATGG 2 cut(s) 426, 690
BspACI CCGC 6 cut(s) 22, 36, 54, 239, 473, 880
BspANI GGCC 4 cut(s) 261, 444, 729, 762
BspCNI CTCAG 3 cut(s) 223, 415, 651
BspFNI CGCG 2 cut(s) 307, 473
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 9 cut(s) 283, 432, 465, 499, 544, 598, 661, 741, 769
BspPI GGATC 2 cut(s) 410, 624
BspT107I GGYRCC 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 2 cut(s) 100, 657
BssECI CCNNGG 7 cut(s) 204, 245, 426, 690, 730, 763, 822
BssMI GATC 2 cut(s) 415, 616
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 4 cut(s) 245, 426, 690, 822
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 7 cut(s) 189, 435, 441, 732, 744, 765, 783
Bst4CI ACNGT 1 cut(s) 715
BstAPI GCANNNNNTGC 2 cut(s) 482, 536
BstAUI TGTACA 1 cut(s) 565
BstC8I GCNNGC 6 cut(s) 145, 231, 392, 478, 681, 701
BstDEI CTNAG 3 cut(s) 210, 402, 638
BstDSI CCRYGG 3 cut(s) 204, 426, 690
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 4 cut(s) 266, 465, 485, 532
BstFNI CGCG 2 cut(s) 307, 473
BstHHI GCGC 2 cut(s) 229, 309
BstKTI GATC 2 cut(s) 418, 619
BstMBI GATC 2 cut(s) 415, 616
BstMWI GCNNNNNNNGC 5 cut(s) 144, 165, 482, 536, 866
BstNI CCWGG 7 cut(s) 189, 435, 441, 732, 744, 765, 783
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 8 cut(s) 187, 433, 439, 612, 730, 742, 763, 781
BstUI CGCG 2 cut(s) 307, 473
BstV1I GCAGC 2 cut(s) 326, 856
BsuRI GGCC 4 cut(s) 261, 444, 729, 762
BtgI CCRYGG 3 cut(s) 204, 426, 690
BtsCI GGATG 4 cut(s) 266, 465, 485, 532
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 138, 526
Cac8I GCNNGC 6 cut(s) 145, 231, 392, 478, 681, 701
CciI TCATGA 1 cut(s) 619
CfoI GCGC 2 cut(s) 229, 309
Cfr13I GGNCC 7 cut(s) 260, 282, 431, 437, 660, 728, 761
CsiI ACCWGGT 1 cut(s) 433
Csp6I GTAC 1 cut(s) 566
CviAII CATG 6 cut(s) 26, 120, 371, 427, 620, 691
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 3 cut(s) 210, 402, 638
DpnI GATC 2 cut(s) 417, 618
DpnII GATC 2 cut(s) 415, 616
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 4 cut(s) 245, 426, 690, 822
Eco24I GRGCYC 1 cut(s) 772
Eco47I GGWCC 4 cut(s) 282, 431, 437, 660
Eco57I CTGAAG 1 cut(s) 354
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoO109I RGGNCCY 2 cut(s) 431, 660
EcoRII CCWGG 7 cut(s) 187, 433, 439, 730, 742, 763, 781
EcoT14I CCWWGG 4 cut(s) 245, 426, 690, 822
EcoT22I ATGCAT 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 4 cut(s) 245, 426, 690, 822
FaeI CATG 6 cut(s) 29, 123, 374, 430, 623, 694
FaqI GGGAC 4 cut(s) 135, 444, 673, 841
FatI CATG 6 cut(s) 25, 119, 370, 426, 619, 690
FauNDI CATATG 1 cut(s) 311
Fnu4HI GCNGC 6 cut(s) 23, 37, 55, 340, 474, 870
FokI GGATG 4 cut(s) 253, 472, 472, 539
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 6 cut(s) 23, 37, 55, 340, 474, 870
FspBI CTAG 2 cut(s) 246, 684
GlaI GCGC 2 cut(s) 228, 308
GluI GCNGC 6 cut(s) 23, 37, 55, 340, 474, 870
GsaI CCCAGC 1 cut(s) 776
GsuI CTGGAG 5 cut(s) 210, 315, 674, 726, 765
HaeIII GGCC 4 cut(s) 261, 444, 729, 762
HapII CCGG 2 cut(s) 613, 726
HhaI GCGC 2 cut(s) 229, 309
Hin1II CATG 6 cut(s) 29, 123, 374, 430, 623, 694
Hin6I GCGC 2 cut(s) 227, 307
HinP1I GCGC 2 cut(s) 227, 307
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 4 cut(s) 83, 220, 623, 673
HpaII CCGG 2 cut(s) 613, 726
HphI GGTGA 2 cut(s) 362, 386
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 3 cut(s) 213, 405, 591
Hpy188III TCNNGA 5 cut(s) 61, 294, 620, 640, 888
Hpy8I GTNNAC 1 cut(s) 566
HpyAV CCTTC 3 cut(s) 23, 329, 550
HpyCH4III ACNGT 1 cut(s) 715
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 165, 482, 536, 866
HpyF3I CTNAG 3 cut(s) 210, 402, 638
Hsp92II CATG 6 cut(s) 29, 123, 374, 430, 623, 694
HspAI GCGC 2 cut(s) 227, 307
Kzo9I GATC 2 cut(s) 415, 616
LmnI GCTCC 5 cut(s) 463, 542, 596, 745, 767
Lsp1109I GCAGC 2 cut(s) 326, 856
LweI GCATC 3 cut(s) 494, 517, 539
MabI ACCWGGT 1 cut(s) 433
MaeI CTAG 2 cut(s) 246, 684
MaeIII GTNAC 4 cut(s) 194, 350, 788, 851
MalI GATC 2 cut(s) 417, 618
MboI GATC 2 cut(s) 415, 616
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 772
MlyI GAGTC 2 cut(s) 92, 632
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 2 cut(s) 30, 48
MspI CCGG 2 cut(s) 613, 726
MspR9I CCNGG 8 cut(s) 189, 435, 441, 614, 732, 744, 765, 783
MvaI CCWGG 7 cut(s) 189, 435, 441, 732, 744, 765, 783
MvnI CGCG 2 cut(s) 307, 473
MwoI GCNNNNNNNGC 5 cut(s) 144, 165, 482, 536, 866
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 2 cut(s) 426, 690
NdeI CATATG 1 cut(s) 311
NdeII GATC 2 cut(s) 415, 616
NlaIII CATG 6 cut(s) 29, 123, 374, 430, 623, 694
NlaIV GGNNCC 9 cut(s) 283, 432, 465, 499, 544, 598, 661, 741, 769
NmuCI GTSAC 2 cut(s) 350, 851
NsiI ATGCAT 1 cut(s) 532
PagI TCATGA 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 220, 673
PflFI GACNNNGTC 1 cut(s) 435
PfoI TCCNGGA 4 cut(s) 187, 612, 742, 781
PkrI GCNGC 6 cut(s) 24, 38, 56, 341, 475, 871
PleI GAGTC 2 cut(s) 91, 631
PpsI GAGTC 2 cut(s) 91, 631
PpuMI RGGWCCY 2 cut(s) 431, 660
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 2 cut(s) 431, 660
Psp6I CCWGG 7 cut(s) 187, 433, 439, 730, 742, 763, 781
PspFI CCCAGC 1 cut(s) 772
PspGI CCWGG 7 cut(s) 187, 433, 439, 730, 742, 763, 781
PspN4I GGNNCC 9 cut(s) 283, 432, 465, 499, 544, 598, 661, 741, 769
PspPI GGNCC 7 cut(s) 260, 282, 431, 437, 660, 728, 761
PspPPI RGGWCCY 2 cut(s) 431, 660
PsyI GACNNNGTC 1 cut(s) 435
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 2 cut(s) 30, 48
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 6 cut(s) 23, 37, 55, 340, 474, 870
Sau3AI GATC 2 cut(s) 415, 616
Sau96I GGNCC 7 cut(s) 260, 282, 431, 437, 660, 728, 761
SchI GAGTC 2 cut(s) 92, 632
ScrFI CCNGG 8 cut(s) 189, 435, 441, 614, 732, 744, 765, 783
SduI GDGCHC 1 cut(s) 772
SexAI ACCWGGT 1 cut(s) 433
SfaNI GCATC 3 cut(s) 494, 517, 539
SinI GGWCC 4 cut(s) 282, 431, 437, 660
SmiMI CAYNNNNRTG 2 cut(s) 30, 48
SsiI CCGC 6 cut(s) 22, 36, 54, 239, 473, 880
SspMI CTAG 2 cut(s) 246, 684
StyD4I CCNGG 8 cut(s) 187, 433, 439, 612, 730, 742, 763, 781
StyI CCWWGG 4 cut(s) 245, 426, 690, 822
TaaI ACNGT 1 cut(s) 715
TaqI TCGA 1 cut(s) 113
TatI WGTACW 1 cut(s) 565
TauI GCSGC 4 cut(s) 25, 39, 57, 476
TfiI GAWTC 2 cut(s) 220, 673
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 145, 526
TseFI GTSAC 2 cut(s) 350, 851
TseI GCWGC 2 cut(s) 339, 869
Tsp45I GTSAC 2 cut(s) 350, 851
TspDTI ATGAA 3 cut(s) 42, 233, 279
TspRI CASTG 2 cut(s) 145, 526
Tth111I GACNNNGTC 1 cut(s) 435
VpaK11BI GGWCC 4 cut(s) 282, 431, 437, 660
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 128, 344
XmaJI CCTAGG 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 2 cut(s) 246, 684
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.