pycom12g05140

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001285
Physical Location & Seq
Reverse (-)
5024542 .. 5025177
636 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05140.1

Sequence Viewer

Length: 636 bp
ATGGATCGAATTCATCTGGAGTTAAATCACGCATATGCAGTAAATATAATTCTCCTGAAGGCTGCAAATTTGGTGACAAATGTCATTTTGCTCATGGTGAGTGGGAACTCGGCAAGCCTCTTGCTAACAGCTTGTCCCACGATCGATCCTCGTGCCATGGGACCTGGTCCAGGCCGTATGGGTAATTGGATGGAGCCACACCTGGCAGGCCCTGCATCTAGCTTTGGTGCCTCATCTACAGCAAAAATCAGTGTGGATGCCTCACATGCAGGAGCCATAATTGGGAAGGGTGGTGTGCATTCGAAGCAGATTTGTCTCCAGACAGGAGCCAAGCTTTCAATCCGGGACCATGAGTCAAATCCCAATCTCAGGAACATTGAACTTGAGGGGACCTTTGAACAGATTCAGCAAGCTAGTGCCATGGTAAGCAAGCTAATTGCGACTGTTTCAACCTCTGGCCCTGGAAAGGCTCCAGGAGGAGGAGGAGGAGGAGGAGGAGGCCGAGGAGCCCCAGCCCCTCCCAGAAGTAACTATAAGACGAAACTATGTGATAATTTTACCAAAGGGATTTGCACCTTTGGAGAAAGATGTCACTTTGCACACGGGGCTGCTGAATTGCGGAAGTCTGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 zf-CCCH zf-CCCH_2

Protein Analysis

212

Amino Acids

21.7

Weight (kDa)

9.37

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 80 - 143 2.9e-13 KH domain
zf-CCCH PF00642 178 - 202 4.3e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 227
AciI CCGC 1 cut(s) 619
AclWI GGATC 2 cut(s) 12, 140
AcsI RAATTY 2 cut(s) 9, 67
AcuI CTGAAG 1 cut(s) 77
AfiI CCNNNNNNNGG 5 cut(s) 170, 282, 369, 466, 479
AgsI TTSAA 4 cut(s) 339, 380, 398, 450
AhdI GACNNNNNGTC 1 cut(s) 352
AjnI CCWGG 5 cut(s) 163, 169, 201, 460, 472
AluBI AGCT 5 cut(s) 131, 222, 334, 413, 433
AluI AGCT 5 cut(s) 131, 222, 334, 413, 433
Alw26I GTCTC 1 cut(s) 320
AlwI GGATC 2 cut(s) 12, 140
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 4 cut(s) 172, 208, 457, 499
ApeKI GCWGC 2 cut(s) 62, 608
ApoI RAATTY 2 cut(s) 9, 67
Asp700I GAANNNNTTC 1 cut(s) 402
AspS9I GGNCC 6 cut(s) 161, 167, 209, 346, 390, 458
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 85, 109
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 4 cut(s) 161, 167, 346, 390
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 511
BauI CACGAG 1 cut(s) 150
BbvI GCAGC 2 cut(s) 49, 595
BccI CCATC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 159
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 5 cut(s) 165, 171, 203, 462, 474
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 2 cut(s) 219, 414
BfmI CTRYAG 1 cut(s) 237
BisI GCNGC 2 cut(s) 63, 609
BlsI GCNGC 2 cut(s) 64, 610
Bme1390I CCNGG 6 cut(s) 165, 171, 203, 344, 462, 474
Bme18I GGWCC 4 cut(s) 161, 167, 346, 390
BmeRI GACNNNNNGTC 1 cut(s) 352
BmgT120I GGNCC 6 cut(s) 161, 167, 209, 346, 390, 458
BmiI GGNNCC 9 cut(s) 162, 195, 229, 274, 328, 347, 391, 471, 508
BmrFI CCNGG 6 cut(s) 165, 171, 203, 344, 462, 474
BmsI GCATC 2 cut(s) 224, 247
BoxI GACNNNNGTC 1 cut(s) 80
BpmI CTGGAG 3 cut(s) 38, 302, 456
Bpu14I TTCGAA 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 344
Bsa29I ATCGAT 1 cut(s) 144
BsaJI CCNNGG 4 cut(s) 156, 420, 460, 502
Bsc4I CCNNNNNNNGG 5 cut(s) 170, 282, 369, 466, 479
BseBI CCWGG 5 cut(s) 165, 171, 203, 462, 474
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 4 cut(s) 156, 420, 460, 502
BseGI GGATG 2 cut(s) 195, 262
BseLI CCNNNNNNNGG 5 cut(s) 170, 282, 369, 466, 479
BseMII CTCAG 1 cut(s) 382
BseRI GAGGAG 8 cut(s) 492, 495, 498, 501, 504, 507, 510, 519
BseXI GCAGC 2 cut(s) 49, 595
BseYI CCCAGC 1 cut(s) 511
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 174, 210, 459, 501
BshNI GGYRCC 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 144
BsiEI CGRYCG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 343
BslFI GGGAC 4 cut(s) 120, 174, 359, 403
BslI CCNNNNNNNGG 5 cut(s) 170, 282, 369, 466, 479
BsmAI GTCTC 1 cut(s) 320
BsmFI GGGAC 4 cut(s) 120, 174, 359, 403
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 4 cut(s) 174, 210, 459, 501
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 511
Bsp143I GATC 3 cut(s) 4, 141, 145
Bsp19I CCATGG 2 cut(s) 156, 420
BspACI CCGC 1 cut(s) 619
BspANI GGCC 4 cut(s) 174, 210, 459, 501
BspCNI CTCAG 1 cut(s) 381
BspDI ATCGAT 1 cut(s) 144
BspLI GGNNCC 9 cut(s) 162, 195, 229, 274, 328, 347, 391, 471, 508
BspPI GGATC 2 cut(s) 12, 140
BspT104I TTCGAA 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 227
BssECI CCNNGG 4 cut(s) 156, 420, 460, 502
BssMI GATC 3 cut(s) 4, 141, 145
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 156, 420
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 5 cut(s) 165, 171, 203, 462, 474
Bst4CI ACNGT 1 cut(s) 445
BstAPI GCANNNNNTGC 1 cut(s) 212
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 4 cut(s) 115, 208, 411, 431
BstDEI CTNAG 1 cut(s) 368
BstDSI CCRYGG 2 cut(s) 156, 420
BstENI CCTNNNNNAGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 195, 262
BstKTI GATC 3 cut(s) 7, 144, 148
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 3 cut(s) 4, 141, 145
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 212, 266, 304, 605
BstNI CCWGG 5 cut(s) 165, 171, 203, 462, 474
BstNSI RCATGY 1 cut(s) 269
BstPAI GACNNNNGTC 1 cut(s) 80
BstSCI CCNGG 6 cut(s) 163, 169, 201, 342, 460, 472
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 49, 595
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 4 cut(s) 174, 210, 459, 501
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 2 cut(s) 156, 420
BtsCI GGATG 2 cut(s) 195, 262
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 115, 208, 411, 431
CaiI CAGNNNCTG 1 cut(s) 212
Cfr13I GGNCC 6 cut(s) 161, 167, 209, 346, 390, 458
ClaI ATCGAT 1 cut(s) 144
CsiI ACCWGGT 1 cut(s) 163
CviAII CATG 5 cut(s) 94, 157, 266, 350, 421
DdeI CTNAG 1 cut(s) 368
DpnI GATC 3 cut(s) 6, 143, 147
DpnII GATC 3 cut(s) 4, 141, 145
DriI GACNNNNNGTC 1 cut(s) 352
Eam1105I GACNNNNNGTC 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 156, 420
Eco24I GRGCYC 1 cut(s) 511
Eco47I GGWCC 4 cut(s) 161, 167, 346, 390
Eco57I CTGAAG 1 cut(s) 77
EcoNI CCTNNNNNAGG 1 cut(s) 168
EcoO109I RGGNCCY 3 cut(s) 161, 209, 390
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 5 cut(s) 163, 169, 201, 460, 472
EcoT14I CCWWGG 2 cut(s) 156, 420
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 2 cut(s) 156, 420
FaeI CATG 5 cut(s) 97, 160, 269, 353, 424
FaqI GGGAC 4 cut(s) 120, 174, 359, 403
FatI CATG 5 cut(s) 93, 156, 265, 349, 420
FauNDI CATATG 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 63, 609
FokI GGATG 2 cut(s) 202, 269
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 2 cut(s) 63, 609
FspBI CTAG 2 cut(s) 219, 414
GluI GCNGC 2 cut(s) 63, 609
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 3 cut(s) 38, 302, 456
HaeIII GGCC 4 cut(s) 174, 210, 459, 501
HapII CCGG 1 cut(s) 343
Hin1II CATG 5 cut(s) 97, 160, 269, 353, 424
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 2 cut(s) 353, 403
HpaII CCGG 1 cut(s) 343
HphI GGTGA 2 cut(s) 85, 109
Hpy188III TCNNGA 5 cut(s) 17, 55, 319, 370, 627
HpyAV CCTTC 2 cut(s) 52, 280
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4V TGCA 7 cut(s) 38, 65, 215, 269, 298, 573, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 266, 304, 605
HpyF3I CTNAG 1 cut(s) 368
Hsp92II CATG 5 cut(s) 97, 160, 269, 353, 424
Kzo9I GATC 3 cut(s) 4, 141, 145
LmnI GCTCC 5 cut(s) 193, 272, 326, 475, 506
Lsp1109I GCAGC 2 cut(s) 49, 595
LweI GCATC 2 cut(s) 224, 247
MabI ACCWGGT 1 cut(s) 163
MaeI CTAG 2 cut(s) 219, 414
MaeIII GTNAC 3 cut(s) 73, 527, 590
MalI GATC 3 cut(s) 6, 143, 147
MboI GATC 3 cut(s) 4, 141, 145
MhlI GDGCHC 1 cut(s) 511
MluCI AATT 8 cut(s) 9, 48, 67, 184, 279, 435, 553, 614
MlyI GAGTC 1 cut(s) 362
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 6 cut(s) 165, 171, 203, 344, 462, 474
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 5 cut(s) 165, 171, 203, 462, 474
MwoI GCNNNNNNNGC 4 cut(s) 212, 266, 304, 605
NciI CCSGG 1 cut(s) 344
NcoI CCATGG 2 cut(s) 156, 420
NdeI CATATG 1 cut(s) 34
NdeII GATC 3 cut(s) 4, 141, 145
NlaIII CATG 5 cut(s) 97, 160, 269, 353, 424
NlaIV GGNNCC 9 cut(s) 162, 195, 229, 274, 328, 347, 391, 471, 508
NmeAIII GCCGAG 2 cut(s) 89, 527
NmuCI GTSAC 2 cut(s) 73, 590
NspI RCATGY 1 cut(s) 269
NspV TTCGAA 1 cut(s) 302
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 1 cut(s) 403
PflFI GACNNNGTC 1 cut(s) 165
PfoI TCCNGGA 2 cut(s) 342, 472
PkrI GCNGC 2 cut(s) 64, 610
Ple19I CGATCG 1 cut(s) 144
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
PpuMI RGGWCCY 2 cut(s) 161, 390
PshAI GACNNNNGTC 1 cut(s) 80
Psp5II RGGWCCY 2 cut(s) 161, 390
Psp6I CCWGG 5 cut(s) 163, 169, 201, 460, 472
PspFI CCCAGC 1 cut(s) 511
PspGI CCWGG 5 cut(s) 163, 169, 201, 460, 472
PspN4I GGNNCC 9 cut(s) 162, 195, 229, 274, 328, 347, 391, 471, 508
PspPI GGNCC 6 cut(s) 161, 167, 209, 346, 390, 458
PspPPI RGGWCCY 2 cut(s) 161, 390
PstNI CAGNNNCTG 1 cut(s) 212
PsyI GACNNNGTC 1 cut(s) 165
PvuI CGATCG 1 cut(s) 144
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 2 cut(s) 63, 609
Sau3AI GATC 3 cut(s) 4, 141, 145
Sau96I GGNCC 6 cut(s) 161, 167, 209, 346, 390, 458
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 6 cut(s) 165, 171, 203, 344, 462, 474
SduI GDGCHC 1 cut(s) 511
SexAI ACCWGGT 1 cut(s) 163
SfaNI GCATC 2 cut(s) 224, 247
SfcI CTRYAG 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 4 cut(s) 161, 167, 346, 390
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 8 cut(s) 9, 48, 67, 184, 279, 435, 553, 614
SsiI CCGC 1 cut(s) 619
SspMI CTAG 2 cut(s) 219, 414
StyD4I CCNGG 6 cut(s) 163, 169, 201, 342, 460, 472
StyI CCWWGG 2 cut(s) 156, 420
TaaI ACNGT 1 cut(s) 445
TaqI TCGA 3 cut(s) 7, 144, 302
TasI AATT 8 cut(s) 9, 48, 67, 184, 279, 435, 553, 614
TfiI GAWTC 1 cut(s) 403
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 2 cut(s) 73, 590
TseI GCWGC 2 cut(s) 62, 608
Tsp45I GTSAC 2 cut(s) 73, 590
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 1 cut(s) 165
VpaK11BI GGWCC 4 cut(s) 161, 167, 346, 390
XagI CCTNNNNNAGG 1 cut(s) 168
XapI RAATTY 2 cut(s) 9, 67
XceI RCATGY 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 2 cut(s) 219, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.