MD01G1037200.v1.1

ZF-HD protein dimerisation region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002915
Physical Location & Seq
Forward (+)
12886256 .. 12888351
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1037200.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGAGTTTAACGATCAAGAGGACCACGAAGAGGAGATGGAGATGACGGCGAATTACGACTCGCTTGGAAACTCCGCCGGAGGCCGAGTCAAAATGTCGAGTTCTGGACCAGATGGTTTGGCGCTGGCAGCAGCAGCTGCGGCGGCGACTCAGCAGCAGCAGCAACCGAGGAAGGTTGTGAGGTACAGAGAGTGCTTAAAGAACCACGCGGTGGGGATTGGAGGCCACGCGCTGGACGGGTGCTGCAAGTTCTTGGCGGCCGGCCCCGAGGGCACGCTCGACGCGCTCAAGTGCGCCGCCTGCAACTGCCACCGCAACTTTCACCGAAAGGAGGGTGACCCGCCCGGACACGGTCTCGGAGTCATAACCGACGCGTGCGGGCAACTGGTACCGCACCACGGGCAGCAACACCACCCCCAGTTCTCGCCGTACTACCGGACTCCAGCTGGGTTTCTACACGTGGCGGCGCACCACAGGCTGCTGGCTTTGCCGTCGACTTCGGGAGGGGGAGGGACCCACAGCGGGGGGCAAGAGCAGGATGACGACGTGTCAAATCCCAGCGGGGGCGGTGGCGGAGGGAGTGGGGGTTTGGGAATGGGGATGGGTATGCACGGAACGGCGTCGTCGGGGAAGAAGAGATTCAGGACAAAGTTCAGTCAGGAGCAGAAAGAGAGGATGTTGAGCTTGGCGGAGAGGCTGGGGTGGAGGATTCAGAAGCAGGATGAACCGGCGGTTCAGCAGTTCTGCAACGAGACTGGAGTGAATCGCCATGTGCTCAAGGTTTGGATGCATAACAACAAACACACTCTGGCAATTTGGAGAAGGATTTGCCTGAAACGGTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ZF-HD_dimer

Protein Analysis

283

Amino Acids

30.45

Weight (kDa)

8.57

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 60 - 111 5.1e-28 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 388
AccB7I CCANNNNNTGG 2 cut(s) 211, 232
AccI GTMKAC 1 cut(s) 494
AccII CGCG 4 cut(s) 209, 230, 284, 374
AcoI YGGCCR 1 cut(s) 258
AcvI CACGTG 1 cut(s) 460
AcyI GRCGYC 1 cut(s) 620
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 3 cut(s) 185, 390, 431
AfiI CCNNNNNNNGG 9 cut(s) 31, 211, 232, 331, 350, 398, 522, 523, 563
AflIII ACRYGT 3 cut(s) 372, 457, 546
AgsI TTSAA 1 cut(s) 844
AhdI GACNNNNNGTC 1 cut(s) 547
AjiI CACGTC 1 cut(s) 547
AluBI AGCT 3 cut(s) 137, 446, 684
AluI AGCT 3 cut(s) 137, 446, 684
Alw21I GWGCWC 1 cut(s) 777
Alw26I GTCTC 2 cut(s) 359, 746
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 4 cut(s) 82, 223, 258, 262
Asp700I GAANNNNTTC 2 cut(s) 638, 839
Asp718I GGTACC 1 cut(s) 388
AspLEI GCGC 5 cut(s) 124, 232, 286, 296, 469
AspS9I GGNCC 4 cut(s) 22, 107, 263, 513
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 314, 347
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 3 cut(s) 22, 107, 513
BaeGI GKGCMC 1 cut(s) 275
BanI GGYRCC 1 cut(s) 388
BbrPI CACGTG 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 777
BccI CCATC 3 cut(s) 31, 107, 595
BceAI ACGGC 4 cut(s) 63, 412, 475, 633
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 359, 746
BfoI RGCGCY 1 cut(s) 125
BglI GCCNNNNNGGC 1 cut(s) 270
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 3 cut(s) 22, 107, 513
BmeRI GACNNNNNGTC 1 cut(s) 547
BmeT110I CYCGRG 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 4 cut(s) 22, 107, 263, 513
BmiI GGNNCC 4 cut(s) 265, 390, 514, 515
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 1 cut(s) 777
BmuI ACTGGG 1 cut(s) 412
BpmI CTGGAG 2 cut(s) 426, 777
BpuEI CTTGAG 2 cut(s) 272, 761
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 167, 267, 397
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 4 cut(s) 72, 102, 571, 601
Bsc4I CCNNNNNNNGG 9 cut(s) 31, 211, 232, 331, 350, 398, 522, 523, 563
Bse118I RCCGGY 2 cut(s) 260, 727
Bse1I ACTGG 3 cut(s) 390, 418, 760
BseDI CCNNGG 3 cut(s) 167, 267, 397
BseGI GGATG 5 cut(s) 544, 606, 681, 727, 792
BseLI CCNNNNNNNGG 9 cut(s) 31, 211, 232, 331, 350, 398, 522, 523, 563
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 390, 418, 760
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 275
BseX3I CGGCCG 1 cut(s) 258
BseYI CCCAGC 3 cut(s) 446, 557, 697
Bsh1236I CGCG 4 cut(s) 209, 230, 284, 374
Bsh1285I CGRYCG 1 cut(s) 261
BshFI GGCC 4 cut(s) 84, 225, 260, 264
BshNI GGYRCC 1 cut(s) 388
BsiEI CGRYCG 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 777
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 5 cut(s) 78, 261, 345, 436, 728
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 9 cut(s) 31, 211, 232, 331, 350, 398, 522, 523, 563
BsmAI GTCTC 2 cut(s) 359, 746
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 4 cut(s) 84, 225, 260, 264
Bso31I GGTCTC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 275, 777
Bsp143I GATC 1 cut(s) 13
BspANI GGCC 4 cut(s) 84, 225, 260, 264
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 4 cut(s) 209, 230, 284, 374
BspLI GGNNCC 4 cut(s) 265, 390, 514, 515
BspT107I GGYRCC 1 cut(s) 388
BspTNI GGTCTC 1 cut(s) 359
BsrFI RCCGGY 2 cut(s) 260, 727
BsrI ACTGG 3 cut(s) 390, 418, 760
BssAI RCCGGY 2 cut(s) 260, 727
BssECI CCNNGG 3 cut(s) 167, 267, 397
BssMI GATC 1 cut(s) 13
BssNI GRCGYC 1 cut(s) 620
Bst4CI ACNGT 2 cut(s) 353, 840
Bst6I CTCTTC 2 cut(s) 24, 629
BstACI GRCGYC 1 cut(s) 620
BstAPI GCANNNNNTGC 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 460
BstC8I GCNNGC 7 cut(s) 126, 262, 275, 301, 376, 380, 483
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 397
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 5 cut(s) 544, 606, 681, 727, 792
BstFNI CGCG 4 cut(s) 209, 230, 284, 374
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 5 cut(s) 124, 232, 286, 296, 469
BstKTI GATC 1 cut(s) 16
BstMAI GTCTC 2 cut(s) 359, 746
BstMBI GATC 1 cut(s) 13
BstMCI CGRYCG 1 cut(s) 261
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 4 cut(s) 209, 230, 284, 374
BstZI CGGCCG 1 cut(s) 258
BsuRI GGCC 4 cut(s) 84, 225, 260, 264
BtgI CCRYGG 1 cut(s) 397
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 5 cut(s) 544, 606, 681, 727, 792
Cac8I GCNNGC 7 cut(s) 126, 262, 275, 301, 376, 380, 483
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 5 cut(s) 124, 232, 286, 296, 469
Cfr10I RCCGGY 2 cut(s) 260, 727
Cfr13I GGNCC 4 cut(s) 22, 107, 263, 513
CseI GACGC 3 cut(s) 290, 380, 609
Csp6I GTAC 3 cut(s) 184, 389, 430
CviAII CATG 1 cut(s) 770
CviQI GTAC 3 cut(s) 184, 389, 430
DdeI CTNAG 1 cut(s) 150
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
DraIII CACNNNGTG 1 cut(s) 211
DriI GACNNNNNGTC 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 258
EagI CGGCCG 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 24, 629
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 2 cut(s) 24, 629
EciI GGCGGA 3 cut(s) 64, 588, 704
EclXI CGGCCG 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 359
Eco47I GGWCC 3 cut(s) 22, 107, 513
Eco52I CGGCCG 1 cut(s) 258
Eco72I CACGTG 1 cut(s) 460
Eco88I CYCGRG 1 cut(s) 266
Eco91I GGTNACC 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 513
EcoO65I GGTNACC 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 792
FaeI CATG 1 cut(s) 773
FaiI YATR 4 cut(s) 365, 608, 771, 792
FaqI GGGAC 1 cut(s) 526
FatI CATG 1 cut(s) 769
FauI CCCGC 4 cut(s) 348, 371, 515, 554
FblI GTMKAC 1 cut(s) 494
FokI GGATG 5 cut(s) 551, 613, 688, 734, 799
FseI GGCCGGCC 1 cut(s) 264
GlaI GCGC 5 cut(s) 123, 231, 285, 295, 468
GsaI CCCAGC 3 cut(s) 450, 561, 701
GsuI CTGGAG 2 cut(s) 426, 777
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 4 cut(s) 84, 225, 260, 264
HapII CCGG 5 cut(s) 78, 261, 345, 436, 728
HgaI GACGC 3 cut(s) 290, 380, 609
HhaI GCGC 5 cut(s) 124, 232, 286, 296, 469
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 1 cut(s) 773
Hin6I GCGC 5 cut(s) 122, 230, 284, 294, 467
HinP1I GCGC 5 cut(s) 122, 230, 284, 294, 467
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HinfI GANTC 8 cut(s) 59, 87, 148, 360, 439, 639, 709, 763
HpaII CCGG 5 cut(s) 78, 261, 345, 436, 728
HphI GGTGA 2 cut(s) 314, 347
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 2 cut(s) 359, 714
Hpy188III TCNNGA 5 cut(s) 17, 105, 501, 643, 659
Hpy8I GTNNAC 1 cut(s) 495
Hpy99I CGWCG 6 cut(s) 284, 374, 496, 548, 625, 628
HpyAV CCTTC 2 cut(s) 166, 816
HpyCH4III ACNGT 2 cut(s) 353, 840
HpyCH4IV ACGT 2 cut(s) 459, 546
HpyCH4V TGCA 5 cut(s) 246, 303, 610, 747, 790
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 2 cut(s) 459, 546
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 1 cut(s) 773
HspAI GCGC 5 cut(s) 122, 230, 284, 294, 467
KflI GGGWCCC 1 cut(s) 513
KpnI GGTACC 1 cut(s) 392
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Kzo9I GATC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 661
LweI GCATC 1 cut(s) 777
MaeII ACGT 2 cut(s) 459, 546
MaeIII GTNAC 1 cut(s) 335
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 3 cut(s) 41, 643, 646
MhlI GDGCHC 2 cut(s) 275, 777
MluCI AATT 2 cut(s) 52, 813
MluI ACGCGT 1 cut(s) 372
MlyI GAGTC 5 cut(s) 53, 96, 142, 369, 433
Mph1103I ATGCAT 1 cut(s) 792
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 638, 839
MseI TTAA 2 cut(s) 9, 197
MspA1I CMGCKG 4 cut(s) 137, 446, 522, 561
MspI CCGG 5 cut(s) 78, 261, 345, 436, 728
MspR9I CCNGG 1 cut(s) 345
MvnI CGCG 4 cut(s) 209, 230, 284, 374
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 13
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 1 cut(s) 773
NlaIV GGNNCC 4 cut(s) 265, 390, 514, 515
NmeAIII GCCGAG 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 335
NsiI ATGCAT 1 cut(s) 792
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 2 cut(s) 638, 839
PfeI GAWTC 3 cut(s) 639, 709, 763
PflFI GACNNNGTC 1 cut(s) 351
PflMI CCANNNNNTGG 2 cut(s) 211, 232
PleI GAGTC 5 cut(s) 53, 95, 142, 368, 433
PmaCI CACGTG 1 cut(s) 460
PmlI CACGTG 1 cut(s) 460
PpsI GAGTC 5 cut(s) 53, 95, 142, 368, 433
Ppu21I YACGTR 1 cut(s) 460
PpuMI RGGWCCY 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 513
PspCI CACGTG 1 cut(s) 460
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 3 cut(s) 446, 557, 697
PspN4I GGNNCC 4 cut(s) 265, 390, 514, 515
PspPI GGNCC 4 cut(s) 22, 107, 263, 513
PspPPI RGGWCCY 1 cut(s) 513
PstNI CAGNNNCTG 1 cut(s) 137
PsyI GACNNNGTC 1 cut(s) 351
PvuII CAGCTG 2 cut(s) 137, 446
RigI GGCCGGCC 1 cut(s) 264
RsaI GTAC 3 cut(s) 185, 390, 431
RsaNI GTAC 3 cut(s) 184, 389, 430
SalI GTCGAC 1 cut(s) 493
SaqAI TTAA 2 cut(s) 9, 197
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 4 cut(s) 22, 107, 263, 513
SchI GAGTC 5 cut(s) 53, 96, 142, 369, 433
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 275, 777
SetI ASST 8 cut(s) 139, 177, 185, 448, 462, 549, 686, 783
SfaNI GCATC 1 cut(s) 777
SinI GGWCC 3 cut(s) 22, 107, 513
SmlI CTYRAG 2 cut(s) 287, 776
SmoI CTYRAG 2 cut(s) 287, 776
Sse9I AATT 2 cut(s) 52, 813
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 353, 840
TaiI ACGT 2 cut(s) 462, 549
TaqI TCGA 3 cut(s) 98, 279, 494
TasI AATT 2 cut(s) 52, 813
TauI GCSGC 5 cut(s) 143, 146, 260, 299, 467
TfiI GAWTC 3 cut(s) 639, 709, 763
Tru1I TTAA 2 cut(s) 9, 197
Tru9I TTAA 2 cut(s) 9, 197
TseFI GTSAC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 738
TspGWI ACGGA 1 cut(s) 627
Tth111I GACNNNGTC 1 cut(s) 351
Van91I CCANNNNNTGG 2 cut(s) 211, 232
VpaK11BI GGWCC 3 cut(s) 22, 107, 513
XmiI GTMKAC 1 cut(s) 494
XmnI GAANNNNTTC 2 cut(s) 638, 839
Zsp2I ATGCAT 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.