MD08G1192300.v1.1

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002915
Physical Location & Seq
Reverse (-)
24640151 .. 24642134
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1192300.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGAATTTGATGAGCACGAAGAGCAAGACGAGGAGATGGAGATGCAGGCGGCGCCACCGGGTTACGCCTCTGTTGCAGAGAGTTCCAGGCCCAAAATGGGGCCCGCCGGCGAGGAGACCGTCTCCAGTGCTCGAAAAAGGGGGACTGCCGCAGCACCCAATTCCACCACCACCACTACCCTAGCCACCATGGTCCGGTACAGGGAATGCCTCAAGAACCACGCCGTTACCATCGGCGGTCACGCCCTCGACGGCTGTGGCGAGTTTCTCTCTGCGGGGGATGAGGGCACCATCGACGCCCTAAAATGTGCCGCCTGCAACTGCCACCGCAACTTCCATCGCAAGGAGTCCGAGGGCGAGTTAATTCGTCACCACCAGGGTGCCGGTGGGGCCCACCACCACCACCATCAGCATCAATTCTCCTCAGGCTACTGTCGCCCACCGCCGCCGCCTTCTGGCTACCTGATCACCTCGCCGCACGCAAGGCCTACACTAGCCTTGCCTGCGGCATCCATCGGAGGCCGCGGAGGGTCTCATAGTAGGGAAGAGGGAGAGGACGTGTCGAATCCAAGTTCGAGTGGCGGTGGAGGCGGAGGAGGATTTGGGATGAGTAAGAAGAGACACCGGACAAAGTTCACGCAGGAGCAGAAGGAGAAGATGTTGGAGTTTGCGGAGAAAGTTGGGTGGAGGATTCAGAAACACGACGAAGCGGCGATTGAGGAGTTTTGCGGAGAGACTGGAGTGAAGCGCCATGTGTTCAAGGTTTGGATGCACAACAACAAGCACACTCTTGATTAA

Protein Analysis

266

Amino Acids

28.78

Weight (kDa)

6.57

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 65 - 117 1.9e-29 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 52, 287, 380
AccII CGCG 1 cut(s) 525
AcsI RAATTY 1 cut(s) 5
AcyI GRCGYC 2 cut(s) 53, 297
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 6 cut(s) 98, 99, 195, 202, 343, 455
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 1 cut(s) 760
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 86, 375
AleI CACNNNNGTG 1 cut(s) 378
Alw21I GWGCWC 2 cut(s) 18, 133
Alw26I GTCTC 5 cut(s) 110, 127, 537, 613, 728
AoxI GGCC 5 cut(s) 89, 101, 390, 485, 520
ApaI GGGCCC 2 cut(s) 105, 394
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 5
AspLEI GCGC 2 cut(s) 55, 750
AspS9I GGNCC 6 cut(s) 90, 101, 102, 193, 390, 391
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 362, 460
AvaII GGWCC 1 cut(s) 193
AxyI CCTNAGG 1 cut(s) 424
BaeGI GKGCMC 3 cut(s) 105, 290, 394
BanI GGYRCC 3 cut(s) 52, 287, 380
BanII GRGCYC 2 cut(s) 105, 394
Bbv12I GWGCWC 2 cut(s) 18, 133
BbvI GCAGC 1 cut(s) 164
BccI CCATC 6 cut(s) 31, 239, 299, 345, 414, 521
BceAI ACGGC 2 cut(s) 209, 268
BciT130I CCWGG 2 cut(s) 88, 377
BclI TGATCA 1 cut(s) 465
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 5 cut(s) 110, 127, 537, 613, 728
BfaI CTAG 2 cut(s) 182, 494
BfoI RGCGCY 2 cut(s) 56, 751
BglI GCCNNNNNGGC 1 cut(s) 389
Bme1390I CCNGG 3 cut(s) 60, 88, 377
Bme18I GGWCC 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 6 cut(s) 90, 101, 102, 193, 390, 391
BmiI GGNNCC 7 cut(s) 54, 102, 103, 289, 382, 391, 392
BmrFI CCNGG 3 cut(s) 60, 88, 377
BmsI GCATC 4 cut(s) 33, 421, 518, 759
BplI GAGNNNNNCTC 4 cut(s) 107, 139, 254, 286
BpmI CTGGAG 2 cut(s) 109, 759
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 60
BsaHI GRCGYC 2 cut(s) 53, 297
BsaI GGTCTC 2 cut(s) 110, 537
BsaJI CCNNGG 4 cut(s) 189, 351, 376, 523
BsaWI WCCGGW 2 cut(s) 195, 624
BsaXI ACNNNNNCTCC 4 cut(s) 543, 573, 644, 674
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 99, 195, 202, 343, 455
Bse118I RCCGGY 2 cut(s) 107, 383
Bse1I ACTGG 2 cut(s) 126, 742
Bse21I CCTNAGG 1 cut(s) 424
BseBI CCWGG 2 cut(s) 88, 377
BseDI CCNNGG 4 cut(s) 189, 351, 376, 523
BseGI GGATG 4 cut(s) 286, 509, 612, 774
BseLI CCNNNNNNNGG 6 cut(s) 98, 99, 195, 202, 343, 455
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 2 cut(s) 126, 742
BseRI GAGGAG 5 cut(s) 47, 128, 412, 609, 734
BseSI GKGCMC 3 cut(s) 105, 290, 394
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 5 cut(s) 91, 103, 392, 487, 522
BshNI GGYRCC 3 cut(s) 52, 287, 380
BsiHKAI GWGCWC 2 cut(s) 18, 133
BsiSI CCGG 5 cut(s) 59, 108, 196, 384, 625
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 6 cut(s) 98, 99, 195, 202, 343, 455
BsmAI GTCTC 5 cut(s) 110, 127, 537, 613, 728
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 157
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 5 cut(s) 91, 103, 392, 487, 522
Bso31I GGTCTC 2 cut(s) 110, 537
Bsp120I GGGCCC 2 cut(s) 101, 390
Bsp1286I GDGCHC 5 cut(s) 18, 105, 133, 290, 394
Bsp143I GATC 1 cut(s) 465
Bsp19I CCATGG 1 cut(s) 189
BspANI GGCC 5 cut(s) 91, 103, 392, 487, 522
BspCNI CTCAG 1 cut(s) 437
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 7 cut(s) 54, 102, 103, 289, 382, 391, 392
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 3 cut(s) 52, 287, 380
BspTNI GGTCTC 2 cut(s) 110, 537
BsrFI RCCGGY 2 cut(s) 107, 383
BsrI ACTGG 2 cut(s) 126, 742
BssAI RCCGGY 2 cut(s) 107, 383
BssECI CCNNGG 4 cut(s) 189, 351, 376, 523
BssMI GATC 1 cut(s) 465
BssNI GRCGYC 2 cut(s) 53, 297
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 2 cut(s) 88, 377
Bst4CI ACNGT 2 cut(s) 121, 434
Bst6I CTCTTC 3 cut(s) 15, 540, 611
BstACI GRCGYC 2 cut(s) 53, 297
BstC8I GCNNGC 6 cut(s) 48, 105, 109, 316, 480, 504
BstDEI CTNAG 1 cut(s) 424
BstDSI CCRYGG 2 cut(s) 189, 523
BstF5I GGATG 4 cut(s) 286, 509, 612, 774
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 2 cut(s) 56, 751
BstHHI GCGC 2 cut(s) 55, 750
BstKTI GATC 1 cut(s) 468
BstMAI GTCTC 5 cut(s) 110, 127, 537, 613, 728
BstMBI GATC 1 cut(s) 465
BstMWI GCNNNNNNNGC 8 cut(s) 22, 52, 74, 389, 435, 484, 503, 588
BstNI CCWGG 2 cut(s) 88, 377
BstSCI CCNGG 3 cut(s) 58, 86, 375
BstSLI GKGCMC 3 cut(s) 105, 290, 394
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 424
BsuRI GGCC 5 cut(s) 91, 103, 392, 487, 522
BtgI CCRYGG 2 cut(s) 189, 523
BtgZI GCGATG 1 cut(s) 323
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 4 cut(s) 286, 509, 612, 774
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 6 cut(s) 48, 105, 109, 316, 480, 504
CfoI GCGC 2 cut(s) 55, 750
Cfr10I RCCGGY 2 cut(s) 107, 383
Cfr13I GGNCC 6 cut(s) 90, 101, 102, 193, 390, 391
Cfr42I CCGCGG 1 cut(s) 526
CseI GACGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 199
CviAII CATG 2 cut(s) 190, 752
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 1 cut(s) 424
DinI GGCGCC 1 cut(s) 54
DpnI GATC 1 cut(s) 467
DpnII GATC 1 cut(s) 465
Eam1104I CTCTTC 3 cut(s) 15, 540, 611
EarI CTCTTC 3 cut(s) 15, 540, 611
EciI GGCGGA 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 487
Eco24I GRGCYC 2 cut(s) 105, 394
Eco31I GGTCTC 2 cut(s) 110, 537
Eco47I GGWCC 1 cut(s) 193
Eco81I CCTNAGG 1 cut(s) 424
EcoO109I RGGNCCY 2 cut(s) 101, 390
EcoRII CCWGG 2 cut(s) 86, 375
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 2 cut(s) 105, 394
EgeI GGCGCC 1 cut(s) 54
EheI GGCGCC 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 2 cut(s) 193, 755
FaiI YATR 3 cut(s) 191, 537, 753
FaqI GGGAC 1 cut(s) 157
FatI CATG 2 cut(s) 189, 751
FauI CCCGC 2 cut(s) 112, 268
FbaI TGATCA 1 cut(s) 465
FokI GGATG 4 cut(s) 293, 496, 619, 781
FriOI GRGCYC 2 cut(s) 105, 394
FspBI CTAG 2 cut(s) 182, 494
GlaI GCGC 2 cut(s) 54, 749
GsuI CTGGAG 2 cut(s) 109, 759
HaeII RGCGCY 2 cut(s) 56, 751
HaeIII GGCC 5 cut(s) 91, 103, 392, 487, 522
HapII CCGG 5 cut(s) 59, 108, 196, 384, 625
HgaI GACGC 1 cut(s) 305
HhaI GCGC 2 cut(s) 55, 750
Hin1I GRCGYC 2 cut(s) 53, 297
Hin1II CATG 2 cut(s) 193, 755
Hin6I GCGC 2 cut(s) 53, 748
HinP1I GCGC 2 cut(s) 53, 748
HinfI GANTC 3 cut(s) 347, 565, 691
HpaII CCGG 5 cut(s) 59, 108, 196, 384, 625
HphI GGTGA 2 cut(s) 362, 460
Hpy166II GTNNAC 1 cut(s) 636
Hpy188I TCNGA 3 cut(s) 352, 518, 696
Hpy188III TCNNGA 2 cut(s) 214, 791
Hpy8I GTNNAC 1 cut(s) 636
Hpy99I CGWCG 3 cut(s) 254, 299, 707
HpyAV CCTTC 2 cut(s) 462, 643
HpyCH4III ACNGT 2 cut(s) 121, 434
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 4 cut(s) 46, 77, 318, 772
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 52, 74, 389, 435, 484, 503, 588
HpyF3I CTNAG 1 cut(s) 424
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 2 cut(s) 53, 297
Hsp92II CATG 2 cut(s) 193, 755
HspAI GCGC 2 cut(s) 53, 748
KasI GGCGCC 1 cut(s) 52
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 465
KspI CCGCGG 1 cut(s) 526
Kzo9I GATC 1 cut(s) 465
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 4 cut(s) 33, 421, 518, 759
MaeI CTAG 2 cut(s) 182, 494
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 4 cut(s) 62, 226, 239, 368
MalI GATC 1 cut(s) 467
MboI GATC 1 cut(s) 465
MboII GAAGA 4 cut(s) 32, 557, 628, 667
MhlI GDGCHC 5 cut(s) 18, 105, 133, 290, 394
MluCI AATT 4 cut(s) 5, 160, 363, 416
Mly113I GGCGCC 1 cut(s) 53
MlyI GAGTC 1 cut(s) 356
MmeI TCCRAC 1 cut(s) 642
MreI CGCCGGCG 1 cut(s) 107
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 2 cut(s) 362, 796
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 525
MspI CCGG 5 cut(s) 59, 108, 196, 384, 625
MspR9I CCNGG 3 cut(s) 60, 88, 377
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 2 cut(s) 88, 377
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 8 cut(s) 22, 52, 74, 389, 435, 484, 503, 588
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 53
NciI CCSGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 189
NdeII GATC 1 cut(s) 465
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 2 cut(s) 193, 755
NlaIV GGNNCC 7 cut(s) 54, 102, 103, 289, 382, 391, 392
NmuCI GTSAC 2 cut(s) 239, 368
OliI CACNNNNGTG 1 cut(s) 378
PceI AGGCCT 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 15
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 212
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 565, 691
PleI GAGTC 1 cut(s) 355
PluTI GGCGCC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 86, 375
PspGI CCWGG 2 cut(s) 86, 375
PspN4I GGNNCC 7 cut(s) 54, 102, 103, 289, 382, 391, 392
PspOMI GGGCCC 2 cut(s) 101, 390
PspPI GGNCC 6 cut(s) 90, 101, 102, 193, 390, 391
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 378
SacII CCGCGG 1 cut(s) 526
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 362, 796
Sau3AI GATC 1 cut(s) 465
Sau96I GGNCC 6 cut(s) 90, 101, 102, 193, 390, 391
SchI GAGTC 1 cut(s) 356
ScrFI CCNGG 3 cut(s) 60, 88, 377
SduI GDGCHC 5 cut(s) 18, 105, 133, 290, 394
SetI ASST 4 cut(s) 465, 473, 561, 765
SfaNI GCATC 4 cut(s) 33, 421, 518, 759
SfoI GGCGCC 1 cut(s) 54
Sfr303I CCGCGG 1 cut(s) 526
SgrAI CRCCGGYG 1 cut(s) 107
SgrBI CCGCGG 1 cut(s) 526
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 4 cut(s) 5, 160, 363, 416
SseBI AGGCCT 1 cut(s) 487
SspDI GGCGCC 1 cut(s) 52
SspMI CTAG 2 cut(s) 182, 494
StuI AGGCCT 1 cut(s) 487
StyD4I CCNGG 3 cut(s) 58, 86, 375
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 2 cut(s) 121, 434
TaiI ACGT 1 cut(s) 561
TaqI TCGA 5 cut(s) 133, 249, 294, 563, 575
TasI AATT 4 cut(s) 5, 160, 363, 416
TauI GCSGC 9 cut(s) 53, 152, 314, 448, 451, 478, 509, 525, 713
TfiI GAWTC 2 cut(s) 565, 691
Tru1I TTAA 2 cut(s) 362, 796
Tru9I TTAA 2 cut(s) 362, 796
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 239, 368
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 239, 368
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 5
XcmI CCANNNNNNNNNTGG 2 cut(s) 94, 383
XspI CTAG 2 cut(s) 182, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.