MD15G1316100.v1.1

ZF-HD protein dimerisation region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002915
Physical Location & Seq
Reverse (-)
32327019 .. 32327855
837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1316100.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGAGTTTGAGGATCAAGAGGAGCACGAGGAGGAGATGGAGATGGCGGCTAGCTACGACTCGCTCGGCAACTCAGCCGGAGGCCGAGTCAAAATGTCGAGTTCTGGACCAGATGGTTTGGCGCTAGCAGCGGCTGCTGCAGCGGCGGCGGCGTCTGATCAGCAGCAGCAACCAAGGAAGGCTGTGAGGTACCGGGAGTGCTTGAAGAACCACGCGGTGGGGATTGGAGGCCACGCGCTGGACGGGTGCTGCGAGTTCCTGGCGGCCGGCCCGGACGGCACGCTCGACGCGCTCAAGTGCGCCGCCTGCAACTGCCATCGGAACTTCCACCGAAAGGAGGGCGACCCGGCAGGACACGGTCTCGGAGTCATAACCGATGCGTACGGGCAGCTGGTGCCACACCACGGGCAGCAACACCACCCGCAGTTCTCACCGTACTACAGGACTCCAGCTGGTTACCTCCACGTGGCGGCGCACCACAGGCCGCTGGCTTTGCCGTCTACTTCGGGAGGGGGAGGGACCTACAGCGGGGGGCAGGAGCAGGATGACGACGTGTCGAATCCCAGCGGGGGCGGTGGCGGTGGCGGAGGGAGTGGGGGTTTGGGAATGGGAATGGGTATGCACGGAACGGCGTCCTCTGGGAAGAAGAGGTTTCGGACAAAGTTTAGTCAGGAGCAGAAGGAGAGGATGTTGAGCTTGGCGGAGCGGCTCGGGTGGAGGATTCAGAAGCAGGACGAACCGGCGGTTCAGCAATTCTGTAACGAGACCGGGGTGAAGCGCCACGTGCTCAAGGTTTGGATGCATAACAACAAACACACTCTGGGTAAGAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ZF-HD_dimer

Protein Analysis

279

Amino Acids

29.54

Weight (kDa)

6.99

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 62 - 113 3.6e-28 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 189
AccB1I GGYRCC 2 cut(s) 189, 394
AccB7I CCANNNNNTGG 2 cut(s) 217, 238
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 500
AccII CGCG 3 cut(s) 215, 236, 290
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 264
AcvI CACGTG 2 cut(s) 466, 784
AcyI GRCGYC 2 cut(s) 152, 632
AdeI CACNNNGTG 1 cut(s) 217
AfaI GTAC 3 cut(s) 191, 383, 437
AfiI CCNNNNNNNGG 9 cut(s) 217, 238, 334, 337, 404, 466, 469, 528, 569
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 1 cut(s) 205
AhdI GACNNNNNGTC 1 cut(s) 553
AjiI CACGTC 1 cut(s) 553
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 4 cut(s) 54, 391, 452, 696
AluI AGCT 4 cut(s) 54, 391, 452, 696
Alw21I GWGCWC 2 cut(s) 27, 789
Alw26I GTCTC 2 cut(s) 365, 758
AlwI GGATC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 5 cut(s) 82, 229, 264, 268, 482
ApeKI GCWGC 9 cut(s) 128, 134, 137, 140, 163, 166, 249, 388, 409
Asp718I GGTACC 1 cut(s) 189
AspLEI GCGC 6 cut(s) 124, 238, 292, 302, 475, 780
AspS9I GGNCC 3 cut(s) 107, 269, 519
AsuC2I CCSGG 4 cut(s) 194, 272, 347, 769
AsuHPI GGTGA 2 cut(s) 423, 784
AsuNHI GCTAGC 2 cut(s) 50, 124
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 2 cut(s) 107, 519
BanI GGYRCC 2 cut(s) 189, 394
BauI CACGAG 1 cut(s) 26
BbrPI CACGTG 2 cut(s) 466, 784
Bbv12I GWGCWC 2 cut(s) 27, 789
BbvI GCAGC 9 cut(s) 121, 124, 140, 152, 175, 178, 236, 400, 421
BccI CCATC 4 cut(s) 31, 37, 107, 324
BceAI ACGGC 3 cut(s) 292, 481, 645
BciT130I CCWGG 1 cut(s) 260
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 4 cut(s) 194, 272, 347, 769
BcoDI GTCTC 2 cut(s) 365, 758
BfaI CTAG 3 cut(s) 51, 125, 835
BfmI CTRYAG 3 cut(s) 138, 439, 523
BfoI RGCGCY 2 cut(s) 125, 781
BglI GCCNNNNNGGC 1 cut(s) 276
Bme1390I CCNGG 5 cut(s) 194, 260, 272, 347, 769
Bme18I GGWCC 2 cut(s) 107, 519
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 710
BmgBI CACGTC 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 107, 269, 519
BmiI GGNNCC 3 cut(s) 191, 396, 520
BmrFI CCNGG 5 cut(s) 194, 260, 272, 347, 769
BmsI GCATC 2 cut(s) 367, 789
BmtI GCTAGC 2 cut(s) 54, 128
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 2 cut(s) 278, 773
BpuMI CCSGG 4 cut(s) 194, 272, 347, 769
BsaAI YACGTR 2 cut(s) 466, 784
BsaHI GRCGYC 2 cut(s) 152, 632
BsaI GGTCTC 2 cut(s) 365, 758
BsaJI CCNNGG 3 cut(s) 173, 403, 768
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 583, 613, 674, 704
Bsc4I CCNNNNNNNGG 9 cut(s) 217, 238, 334, 337, 404, 466, 469, 528, 569
Bse118I RCCGGY 2 cut(s) 266, 739
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 3 cut(s) 173, 403, 768
BseGI GGATG 3 cut(s) 550, 693, 804
BseLI CCNNNNNNNGG 9 cut(s) 217, 238, 334, 337, 404, 466, 469, 528, 569
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 3 cut(s) 35, 44, 47
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 9 cut(s) 121, 124, 140, 152, 175, 178, 236, 400, 421
BseYI CCCAGC 1 cut(s) 563
Bsh1236I CGCG 3 cut(s) 215, 236, 290
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 5 cut(s) 84, 231, 266, 270, 484
BshNI GGYRCC 2 cut(s) 189, 394
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 27, 789
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 7 cut(s) 78, 193, 267, 272, 347, 740, 768
BsiWI CGTACG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 9 cut(s) 217, 238, 334, 337, 404, 466, 469, 528, 569
BsmAI GTCTC 2 cut(s) 365, 758
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 5 cut(s) 84, 231, 266, 270, 484
Bso31I GGTCTC 2 cut(s) 365, 758
BsoBI CYCGRG 1 cut(s) 710
Bsp1286I GDGCHC 2 cut(s) 27, 789
Bsp143I GATC 2 cut(s) 13, 157
BspANI GGCC 5 cut(s) 84, 231, 266, 270, 484
BspCNI CTCAG 1 cut(s) 86
BspFNI CGCG 3 cut(s) 215, 236, 290
BspLI GGNNCC 3 cut(s) 191, 396, 520
BspMAI CTGCAG 1 cut(s) 142
BspOI GCTAGC 2 cut(s) 54, 128
BspPI GGATC 1 cut(s) 21
BspT107I GGYRCC 2 cut(s) 189, 394
BspTNI GGTCTC 2 cut(s) 365, 758
BsrBI CCGCTC 1 cut(s) 706
BsrFI RCCGGY 2 cut(s) 266, 739
BssAI RCCGGY 2 cut(s) 266, 739
BssECI CCNNGG 3 cut(s) 173, 403, 768
BssMI GATC 2 cut(s) 13, 157
BssNI GRCGYC 2 cut(s) 152, 632
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 359, 435
Bst6I CTCTTC 1 cut(s) 641
BstACI GRCGYC 2 cut(s) 152, 632
BstAPI GCANNNNNTGC 2 cut(s) 134, 394
BstBAI YACGTR 2 cut(s) 466, 784
BstC8I GCNNGC 6 cut(s) 52, 126, 268, 281, 307, 489
BstDEI CTNAG 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 403
BstEII GGTNACC 1 cut(s) 455
BstF5I GGATG 3 cut(s) 550, 693, 804
BstFNI CGCG 3 cut(s) 215, 236, 290
BstH2I RGCGCY 2 cut(s) 125, 781
BstHHI GCGC 6 cut(s) 124, 238, 292, 302, 475, 780
BstKTI GATC 2 cut(s) 16, 160
BstMAI GTCTC 2 cut(s) 365, 758
BstMBI GATC 2 cut(s) 13, 157
BstMCI CGRYCG 1 cut(s) 267
BstNI CCWGG 1 cut(s) 260
BstPI GGTNACC 1 cut(s) 455
BstSCI CCNGG 5 cut(s) 192, 258, 270, 345, 767
BstSFI CTRYAG 3 cut(s) 138, 439, 523
BstUI CGCG 3 cut(s) 215, 236, 290
BstV1I GCAGC 9 cut(s) 121, 124, 140, 152, 175, 178, 236, 400, 421
BstZI CGGCCG 1 cut(s) 264
BsuRI GGCC 5 cut(s) 84, 231, 266, 270, 484
BtgI CCRYGG 1 cut(s) 403
BtrI CACGTC 1 cut(s) 553
BtsCI GGATG 3 cut(s) 550, 693, 804
Cac8I GCNNGC 6 cut(s) 52, 126, 268, 281, 307, 489
CaiI CAGNNNCTG 1 cut(s) 134
CfoI GCGC 6 cut(s) 124, 238, 292, 302, 475, 780
Cfr10I RCCGGY 2 cut(s) 266, 739
Cfr13I GGNCC 3 cut(s) 107, 269, 519
CseI GACGC 3 cut(s) 141, 296, 621
Csp6I GTAC 3 cut(s) 190, 382, 436
CviQI GTAC 3 cut(s) 190, 382, 436
DdeI CTNAG 1 cut(s) 73
DpnI GATC 2 cut(s) 15, 159
DpnII GATC 2 cut(s) 13, 157
DraIII CACNNNGTG 1 cut(s) 217
DriI GACNNNNNGTC 1 cut(s) 553
EaeI YGGCCR 1 cut(s) 264
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 641
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 1 cut(s) 641
EciI GGCGGA 2 cut(s) 600, 716
EclXI CGGCCG 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 173
Eco31I GGTCTC 2 cut(s) 365, 758
Eco47I GGWCC 2 cut(s) 107, 519
Eco52I CGGCCG 1 cut(s) 264
Eco72I CACGTG 2 cut(s) 466, 784
Eco88I CYCGRG 1 cut(s) 710
Eco91I GGTNACC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 519
EcoO65I GGTNACC 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 173
EcoT22I ATGCAT 1 cut(s) 804
ErhI CCWWGG 1 cut(s) 173
FaiI YATR 3 cut(s) 371, 620, 804
FaqI GGGAC 1 cut(s) 532
FauI CCCGC 3 cut(s) 429, 521, 560
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 1 cut(s) 500
FokI GGATG 3 cut(s) 557, 700, 811
FseI GGCCGGCC 1 cut(s) 270
FspBI CTAG 3 cut(s) 51, 125, 835
GlaI GCGC 6 cut(s) 123, 237, 291, 301, 474, 779
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 2 cut(s) 125, 781
HaeIII GGCC 5 cut(s) 84, 231, 266, 270, 484
HapII CCGG 7 cut(s) 78, 193, 267, 272, 347, 740, 768
HgaI GACGC 3 cut(s) 141, 296, 621
HhaI GCGC 6 cut(s) 124, 238, 292, 302, 475, 780
Hin1I GRCGYC 2 cut(s) 152, 632
Hin6I GCGC 6 cut(s) 122, 236, 290, 300, 473, 778
HinP1I GCGC 6 cut(s) 122, 236, 290, 300, 473, 778
HinfI GANTC 6 cut(s) 59, 87, 366, 445, 559, 721
HpaII CCGG 7 cut(s) 78, 193, 267, 272, 347, 740, 768
HphI GGTGA 2 cut(s) 423, 784
Hpy166II GTNNAC 1 cut(s) 501
Hpy188I TCNGA 5 cut(s) 157, 321, 365, 657, 726
Hpy188III TCNNGA 4 cut(s) 17, 105, 507, 671
Hpy8I GTNNAC 1 cut(s) 501
Hpy99I CGWCG 2 cut(s) 290, 554
HpyAV CCTTC 2 cut(s) 172, 673
HpyCH4III ACNGT 2 cut(s) 359, 435
HpyCH4IV ACGT 3 cut(s) 465, 552, 783
HpyCH4V TGCA 4 cut(s) 140, 309, 622, 802
HpyF3I CTNAG 1 cut(s) 73
HpySE526I ACGT 3 cut(s) 465, 552, 783
Hsp92I GRCGYC 2 cut(s) 152, 632
HspAI GCGC 6 cut(s) 122, 236, 290, 300, 473, 778
KpnI GGTACC 1 cut(s) 193
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 2 cut(s) 13, 157
LmnI GCTCC 4 cut(s) 22, 538, 673, 703
Lsp1109I GCAGC 9 cut(s) 121, 124, 140, 152, 175, 178, 236, 400, 421
LweI GCATC 2 cut(s) 367, 789
MaeI CTAG 3 cut(s) 51, 125, 835
MaeII ACGT 3 cut(s) 465, 552, 783
MaeIII GTNAC 2 cut(s) 455, 758
MalI GATC 2 cut(s) 15, 159
MbiI CCGCTC 1 cut(s) 706
MboI GATC 2 cut(s) 13, 157
MboII GAAGA 3 cut(s) 217, 655, 658
MhlI GDGCHC 2 cut(s) 27, 789
MluCI AATT 1 cut(s) 752
MlyI GAGTC 4 cut(s) 53, 96, 375, 439
Mph1103I ATGCAT 1 cut(s) 804
MroNI GCCGGC 1 cut(s) 266
MspA1I CMGCKG 7 cut(s) 131, 143, 391, 452, 487, 528, 567
MspI CCGG 7 cut(s) 78, 193, 267, 272, 347, 740, 768
MspR9I CCNGG 5 cut(s) 194, 260, 272, 347, 769
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 3 cut(s) 215, 236, 290
NaeI GCCGGC 1 cut(s) 268
NciI CCSGG 4 cut(s) 194, 272, 347, 769
NdeII GATC 2 cut(s) 13, 157
NgoMIV GCCGGC 1 cut(s) 266
NheI GCTAGC 2 cut(s) 50, 124
NlaIV GGNNCC 3 cut(s) 191, 396, 520
NmeAIII GCCGAG 2 cut(s) 45, 110
NsiI ATGCAT 1 cut(s) 804
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 282
PdiI GCCGGC 1 cut(s) 268
PfeI GAWTC 2 cut(s) 559, 721
Pfl23II CGTACG 1 cut(s) 381
PflFI GACNNNGTC 1 cut(s) 357
PflMI CCANNNNNTGG 2 cut(s) 217, 238
PleI GAGTC 4 cut(s) 53, 95, 374, 439
PmaCI CACGTG 2 cut(s) 466, 784
PmlI CACGTG 2 cut(s) 466, 784
PpsI GAGTC 4 cut(s) 53, 95, 374, 439
Ppu21I YACGTR 2 cut(s) 466, 784
PpuMI RGGWCCY 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 258
PspCI CACGTG 2 cut(s) 466, 784
PspEI GGTNACC 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 1 cut(s) 258
PspLI CGTACG 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 191, 396, 520
PspPI GGNCC 3 cut(s) 107, 269, 519
PspPPI RGGWCCY 1 cut(s) 519
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 134
PsyI GACNNNGTC 1 cut(s) 357
PvuII CAGCTG 2 cut(s) 391, 452
RigI GGCCGGCC 1 cut(s) 270
RsaI GTAC 3 cut(s) 191, 383, 437
RsaNI GTAC 3 cut(s) 190, 382, 436
Sau3AI GATC 2 cut(s) 13, 157
Sau96I GGNCC 3 cut(s) 107, 269, 519
SchI GAGTC 4 cut(s) 53, 96, 375, 439
ScrFI CCNGG 5 cut(s) 194, 260, 272, 347, 769
SduI GDGCHC 2 cut(s) 27, 789
SfaNI GCATC 2 cut(s) 367, 789
SfcI CTRYAG 3 cut(s) 138, 439, 523
SinI GGWCC 2 cut(s) 107, 519
SmlI CTYRAG 2 cut(s) 293, 788
SmoI CTYRAG 2 cut(s) 293, 788
Sse9I AATT 1 cut(s) 752
SspMI CTAG 3 cut(s) 51, 125, 835
StyD4I CCNGG 5 cut(s) 192, 258, 270, 345, 767
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 2 cut(s) 359, 435
TaiI ACGT 3 cut(s) 468, 555, 786
TaqI TCGA 3 cut(s) 98, 285, 557
TasI AATT 1 cut(s) 752
TfiI GAWTC 2 cut(s) 559, 721
TseI GCWGC 9 cut(s) 128, 134, 137, 140, 163, 166, 249, 388, 409
TspGWI ACGGA 1 cut(s) 639
Tth111I GACNNNGTC 1 cut(s) 357
Van91I CCANNNNNTGG 2 cut(s) 217, 238
VpaK11BI GGWCC 2 cut(s) 107, 519
XmiI GTMKAC 1 cut(s) 500
XspI CTAG 3 cut(s) 51, 125, 835
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.