MD03G1044500.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002466
Physical Location & Seq
Reverse (-)
3527238 .. 3531810
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1044500.v1.1.491

Sequence Viewer

Length: 3618 bp
ATGATGGGATTCTTTTGCTTTCCGTTGATGTTTGTTTTTCACTTCCACCTGCTCCTAAGTTCGACCTCAGGCGCCAGAACTTTGTCTTCAAGCGGCGGCGGCGGCGGCGGCGATGTGGTGGAGCTGTTGTATGCATTCAAACAATCCTCTGTTGTATCTGATCCCCAGGGCTTCTTATCTGATTGGAAACCTGATTCTTCCACTCCTTTCTGCTCATGGAAAGGCATCACCTGCTCCTCTGACGGCGCAGTCACCTCCCTCAACCTCTCCAACGCCGGTCTCATTGGCGACCTCCACCTTCCGCGTCTCACCACCCTGCCGAACCTCCAGCAACTCCACCTACAGGGCAATTCATTCTCTGGTGCTGATCTCTCTGTGTCCAATTTAACATCATGCAGGCTTGAAACTGTTGACTTGTCCTCCAACAACATCTCCCAACCGCTTCCAGTCCGGTCGTTTCTTCAGGGCTGCGACCGCCTTGTTTTCGCTAACTTGTCGCGAAATTCAATCCCCGGAGGCGGTCTAGGGTTTGGTGCTTCTCTGCTTCAGCTTGACATCTCTCACAATCTGATTTCGAATGCGGACTGGTTGACTTGCAACAATCTGAACCTGCTCAATGTTTCACACAACAAGCTCACCGGAAAGCTGAGCGATTCTTTCTTGTCTTGCAAGAATGTATCAACCCTTGACCTTTCGTACAACAATTTTTCCGGGAAATTGCCAAACAGCTTCATTGCAAAGGCTTCACCTTCTCTCAAGTATTTGGATCTCTCAAGCAACAATTTTTCGGGGAATTTCTCCGCCCTTGATTTCGGGCAGTGCAGCAGCCTCACTTTGCTTAGGCTATCACATAATGCACTCTCTGGGGATCAGTTTCCTCCGAGTTTCGAAAGCTGTCAGGCTCTAGAGACGCTAGACCTGTCGAATAATAAGCTCGAGAATGAAATTCCGGGTGTTTTGTTGGGTAATCTGAAGAAATTGAGACAACTATTTCTTGGTCATAATCGTTTTTCGGGTGCAATTCCTGCCGAACTAGGAAAAGCTTGTGGGACTCTTGAGGAACTCGATATCTCTGATAACACTCTCTCTGGAGAGTTGCCCTCGAGTTTTTTGTCCTGCACTTCATTGGTTAGCCTCAATCTCAGTCACAATCAGCTCTCAGGGAAATTCCTCAATACCGTTGTAAGTAGTCTTCCTAGCTTGAGATATCTCTATGTGCCATTCAACAACATAACTGGTCCTGTGCCGCTGTCTCTTACAAATGGCACGCGACTTCAAGTGCTAGATCTCAGTTCGAATGCCTTCACTGGGAACATTCCTTCAGAGTTTTGCTCCTCCAATGCTGCATCAGTTTTAGAAAAGGTGCTTCTAGCCAACAATGATCTGTCCGGGAATGTGCCCTCGGAGCTTGGAAACTGCAAGAACCTGAAGGCCATTGATCTGAGTTTCAACCATTTGAGTGGTCCAATTCCTTCAGAAATTTGGAGCTTGCCGAAGCTCTCTGACTTGGTTATGTGGGCAAACAATCTCACCGGCGAAATCCCAGAAGGCATTTGCATCAACGGAGGAAACCTGGAAACTCTGATTCTCAACAACAATCTCATCTCTGGAACCATTCCGCGGTCGATTGTCAACTGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGCCTCAGTGGAGATATCCCGTCTGGAATTGGGAATCTCCATAAGCTTGCTATACTCCAGTTGGGCAACAATTCACTCTCGGGACAGATTCCAGCTGAGCTTGGCAAGTGTGAGAACCTCATTTGGCTTGATTTAAATAGCAATGAATTACACGGTTCTATCCCTCCGGAGCTTTCCAACCAAGCCGGCCTAGTCCTTCCCGGGATTGTTTCCGGGAAGCATTTTGCATTCGTAAGAAATGAGGGGGGGACATCTTGCAGGGGTGCAGGAGGACTAGTTGAGTTTGAGGGCATCCGGGCAGAGACACTAGAAAAGTTTCCTATGGTTCACTCGTGCTCGTCAACTAGAATTTACTCTGGATTGACAGTTTACACATTCACCAGCAATGGCAGCATGATCTTCCTTGATCTGTCTTACAATTCCTTGTCCGGAACCATTCCTGAAAACTTAGGTACGCTGTCCTACTTGCAGGTCCTAAACATAGGACACAACATGCTAGGTGGAAACATTCCTGATAGCTTTGGAGGATTGAAAGCAGTTGGAGTCCTTGATCTCTCTCACAACAATCTTCAGGGTTATGTCCCGGGATCGTTGGGGACACTCTCTTTTCTGAATGACCTTGATGTGTCCAACAACAACCTCACTGGCGTCATCCCTTCCGGAGGTCAGCTGACCACTTTCCCAGCATCCAGATATGAGAACAACTCCGGCCTTTGTGGGGTACCCTTGGCGGCATGTAGCTCTCAAAGGCAATCATCAGATTCCAAAGATGGAGGGAAAAAGAATTCTTTGACCTCAGTAATGGTAATTGGTACCACAGTCTTTTTCTTCTGTATCCTTATACTAGCGCTAGTGCTGTATCGGGTGAAGAAGTGCCAGCAGAAGGAGGAAAAGAGAGAGAAATATATCGAAAGCCTTCCAACTTCCGGCAGCAGCAGCTGGAAACTTTGCGGTGTTCCTGAGCCTCTGAGCATCAACGTAGCCACATTCGAGAAACCTTTGAGGAAACTGACATTCGCCCATCTGCTCGAAGCCACCAACGGTTTCAGTGTAGACAGCTTGATTGGCACTGGAGGGTTTGGCGAGGTCTACAAGGCAAAACTGGGAGATGGCTGTGTTGTTGCAATTAAGAAGCTTATTCAAGTCACAGGGCAAGGCGACAGGGAGTTTATGGCAGAAATGGAAACTATTGGGAAAATCAAGCACCGGAACCTGGTTCCTTTGTTGGGGTACTGCAAGATCGGCGAGGAGAGACTTCTTGTGTATGAGTACATGAAATGGGGAAGTCTGGAGGCTGTTCTTCATGACAAGTCTAAGGCAGGGGTTTTGAGGCTTGATTGGGCAGCAAGAAAGAAGATTGCCATAGGGTCTGCGAGAGGTCTAGCATTCCTTCACCATAGTTGCTCACCTCATATTATCCACCGTGACATGAAGTCTAGCAATGTTCTTCTGGATGAAAATTTTGAGGCCAGAGTATCTGATTTTGGCATGGCAAGATTGGTCAATGCCCTCGACACTCATCTCAGTGTGAGCACCCTTGCAGGCACTCCAGGTTATGTTCCTCCTGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGGGATGTCTACAGTTATGGCGTTATTTTGCTAGAGCTTCTCTCTGGTAAAAGGCCTATAGACCCATCAGCGTTTGGTGATGACAACAATCTGGTTGGATGGGCAAAGCTGCTTCAGAGAGAGAAGAGATGGAGTGAGATACTGGACACCGAGTTGTTGGCACAAATATCCGGTGAGGCTGAACTATACCAGTATCTGCGGATTGCCTTTGAATGTCTTGATCACAGGCCATTCCATAGGCCAACCATGATTCAGGTTATGGCTATGTTTAAAGAGCTTCAGGTTGATTCACAGAATGACGTCCTTGATGACTTTTCGCTGAAAGAGACTGTTGTTGAAGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1206

Amino Acids

130.29

Weight (kDa)

5.89

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 79 2.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 293 - 431 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 295 - 337 3.3e-06 Leucine rich repeat
LRR_8 PF13855 351 - 411 1.2e-07 Leucine rich repeat
LRR_14 PF23598 469 - 607 2e-06 Leucine-rich repeat region
Island PF20141 620 - 684 3.7e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 712 - 771 6.1e-08 Leucine rich repeat
Pkinase PF00069 907 - 1178 7.8e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 909 - 1178 1.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 57, 239
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 3573
Acc36I ACCTGC 4 cut(s) 57, 239, 618, 2131
Acc65I GGTACC 2 cut(s) 2392, 2483
AccB1I GGYRCC 3 cut(s) 71, 2392, 2483
AccI GTMKAC 3 cut(s) 2724, 2760, 3279
AccII CGCG 4 cut(s) 304, 499, 1270, 1621
AccIII TCCGGA 3 cut(s) 1837, 2099, 2330
AclWI GGATC 4 cut(s) 155, 774, 876, 2266
AcsI RAATTY 8 cut(s) 502, 793, 945, 1166, 1479, 2019, 2455, 3130
AcuI CTGAAG 9 cut(s) 446, 530, 992, 1305, 1448, 1458, 2225, 3368, 3533
AcyI GRCGYC 3 cut(s) 72, 2319, 3570
AfaI GTAC 7 cut(s) 698, 2125, 2394, 2485, 2903, 2942, 3242
AfeI AGCGCT 1 cut(s) 2520
AhdI GACNNNNNGTC 1 cut(s) 3103
AhlI ACTAGT 1 cut(s) 1945
AjnI CCWGG 4 cut(s) 165, 1572, 2883, 3220
Alw21I GWGCWC 2 cut(s) 2009, 3206
Alw26I GTCTC 8 cut(s) 284, 311, 902, 976, 1257, 1967, 2917, 3590
AlwI GGATC 4 cut(s) 155, 774, 876, 2266
AlwNI CAGNNNCTG 2 cut(s) 2610, 3466
Ama87I CYCGRG 5 cut(s) 935, 1102, 1750, 1871, 2254
Aor13HI TCCGGA 3 cut(s) 1837, 2099, 2330
Aor51HI AGCGCT 1 cut(s) 2520
AoxI GGCC 7 cut(s) 1431, 1858, 2380, 3138, 3323, 3497, 3509
ApoI RAATTY 8 cut(s) 502, 793, 945, 1166, 1479, 2019, 2455, 3130
Asp700I GAANNNNTTC 3 cut(s) 1301, 1986, 2586
Asp718I GGTACC 2 cut(s) 2392, 2483
AspLEI GCGC 3 cut(s) 74, 248, 2521
AspS9I GGNCC 3 cut(s) 1238, 1463, 2143
AsuII TTCGAA 3 cut(s) 575, 888, 1295
AvaI CYCGRG 5 cut(s) 935, 1102, 1750, 1871, 2254
AvaII GGWCC 3 cut(s) 1238, 1463, 2143
AxyI CCTNAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 1401
BaeI ACNNNNGTAYC 2 cut(s) 2475, 2508
BanI GGYRCC 3 cut(s) 71, 2392, 2483
BarI GAAGNNNNNNTAC 2 cut(s) 1176, 1208
BauI CACGAG 1 cut(s) 2002
BbsI GAAGAC 2 cut(s) 78, 1184
Bbv12I GWGCWC 2 cut(s) 2009, 3206
BccI CCATC 6 cut(s) 2435, 2700, 2774, 3343, 3363, 3393
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 4 cut(s) 167, 1574, 2885, 3222
BciVI GTATCC 1 cut(s) 2516
BclI TGATCA 1 cut(s) 3490
BcoDI GTCTC 8 cut(s) 284, 311, 902, 976, 1257, 1967, 2917, 3590
BcuI ACTAGT 1 cut(s) 1945
BfmI CTRYAG 3 cut(s) 341, 3280, 3327
BfoI RGCGCY 2 cut(s) 75, 2522
BfuAI ACCTGC 4 cut(s) 57, 239, 618, 2131
BfuI GTATCC 1 cut(s) 2516
BglII AGATCT 1 cut(s) 1285
BlpI GCTNAGC 2 cut(s) 647, 1767
BmcAI AGTACT 1 cut(s) 3242
Bme18I GGWCC 3 cut(s) 1238, 1463, 2143
BmeRI GACNNNNNGTC 1 cut(s) 3103
BmeT110I CYCGRG 5 cut(s) 935, 1102, 1750, 1871, 2254
BmgT120I GGNCC 3 cut(s) 1238, 1463, 2143
BmiI GGNNCC 7 cut(s) 73, 1612, 2104, 2394, 2485, 2882, 2889
BmrI ACTGGG 2 cut(s) 1317, 2783
BmsI GCATC 7 cut(s) 234, 1355, 1566, 1971, 2366, 2652, 3248
BmuI ACTGGG 2 cut(s) 1317, 2783
BpiI GAAGAC 2 cut(s) 78, 1184
BplI GAGNNNNNCTC 8 cut(s) 1085, 1117, 1316, 1348, 2360, 2392, 3296, 3328
BpmI CTGGAG 6 cut(s) 311, 1110, 1712, 2763, 2981, 3204
Bpu10I CCTNAGC 2 cut(s) 839, 2631
Bpu1102I GCTNAGC 2 cut(s) 647, 1767
Bpu14I TTCGAA 3 cut(s) 575, 888, 1295
BpuEI CTTGAG 4 cut(s) 740, 757, 1076, 1222
BsaHI GRCGYC 3 cut(s) 72, 2319, 3570
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 8 cut(s) 165, 166, 511, 1401, 1619, 1871, 2254, 2397
BsaWI WCCGGW 7 cut(s) 450, 638, 1837, 2099, 2330, 2877, 3440
BsaXI ACNNNNNCTCC 8 cut(s) 113, 143, 404, 416, 434, 446, 1932, 1962
Bse118I RCCGGY 3 cut(s) 275, 1532, 1856
Bse21I CCTNAGG 1 cut(s) 67
Bse3DI GCAATG 4 cut(s) 732, 1819, 2062, 3118
BseAI TCCGGA 3 cut(s) 1837, 2099, 2330
BseBI CCWGG 4 cut(s) 167, 1574, 2885, 3222
BseDI CCNNGG 8 cut(s) 165, 166, 511, 1401, 1619, 1871, 2254, 2397
BseGI GGATG 6 cut(s) 1962, 2322, 2357, 3130, 3280, 3374
BseMI GCAATG 4 cut(s) 732, 1819, 2062, 3118
BseRI GAGGAG 3 cut(s) 226, 1324, 2933
BseSI GKGCMC 1 cut(s) 1401
BseYI CCCAGC 1 cut(s) 2353
BsgI GTGCAG 4 cut(s) 841, 1102, 1621, 1956
Bsh1236I CGCG 4 cut(s) 304, 499, 1270, 1621
Bsh1285I CGRYCG 3 cut(s) 455, 475, 1625
BshFI GGCC 7 cut(s) 1433, 1860, 2382, 3140, 3325, 3499, 3511
BshNI GGYRCC 3 cut(s) 71, 2392, 2483
BsiEI CGRYCG 3 cut(s) 455, 475, 1625
BsiHKAI GWGCWC 2 cut(s) 2009, 3206
BsiHKCI CYCGRG 5 cut(s) 935, 1102, 1750, 1871, 2254
BslFI GGGAC 5 cut(s) 1063, 1767, 1933, 2237, 2281
BsmAI GTCTC 8 cut(s) 284, 311, 902, 976, 1257, 1967, 2917, 3590
BsmBI CGTCTC 2 cut(s) 311, 902
BsmFI GGGAC 5 cut(s) 1063, 1767, 1933, 2237, 2281
BsmI GAATGC 5 cut(s) 134, 583, 1303, 1898, 3056
BsnI GGCC 7 cut(s) 1433, 1860, 2382, 3140, 3325, 3499, 3511
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 5 cut(s) 935, 1102, 1750, 1871, 2254
Bsp119I TTCGAA 3 cut(s) 575, 888, 1295
Bsp1286I GDGCHC 3 cut(s) 1401, 2009, 3206
Bsp13I TCCGGA 3 cut(s) 1837, 2099, 2330
Bsp1720I GCTNAGC 2 cut(s) 647, 1767
Bsp68I TCGCGA 1 cut(s) 499
BspANI GGCC 7 cut(s) 1433, 1860, 2382, 3140, 3325, 3499, 3511
BspEI TCCGGA 3 cut(s) 1837, 2099, 2330
BspFNI CGCG 4 cut(s) 304, 499, 1270, 1621
BspHI TCATGA 1 cut(s) 2974
BspLI GGNNCC 7 cut(s) 73, 1612, 2104, 2394, 2485, 2882, 2889
BspMI ACCTGC 4 cut(s) 57, 239, 618, 2131
BspPI GGATC 4 cut(s) 155, 774, 876, 2266
BspT104I TTCGAA 3 cut(s) 575, 888, 1295
BspT107I GGYRCC 3 cut(s) 71, 2392, 2483
BspTNI GGTCTC 1 cut(s) 284
BsrDI GCAATG 4 cut(s) 732, 1819, 2062, 3118
BsrFI RCCGGY 3 cut(s) 275, 1532, 1856
BssAI RCCGGY 3 cut(s) 275, 1532, 1856
BssECI CCNNGG 8 cut(s) 165, 166, 511, 1401, 1619, 1871, 2254, 2397
BssNI GRCGYC 3 cut(s) 72, 2319, 3570
BssSI CACGAG 1 cut(s) 2002
BssT1I CCWWGG 1 cut(s) 2397
Bst2BI CACGAG 1 cut(s) 2002
Bst2UI CCWGG 4 cut(s) 167, 1574, 2885, 3222
Bst4CI ACNGT 9 cut(s) 409, 1180, 1826, 2038, 2491, 2714, 3095, 3284, 3601
Bst6I CTCTTC 1 cut(s) 3389
BstACI GRCGYC 3 cut(s) 72, 2319, 3570
BstAPI GCANNNNNTGC 2 cut(s) 231, 1025
BstBI TTCGAA 3 cut(s) 575, 888, 1295
BstC8I GCNNGC 7 cut(s) 398, 1268, 1490, 1719, 1858, 2549, 3214
BstDSI CCRYGG 1 cut(s) 1619
BstENI CCTNNNNNAGG 1 cut(s) 2331
BstF5I GGATG 6 cut(s) 1962, 2322, 2357, 3130, 3280, 3374
BstFNI CGCG 4 cut(s) 304, 499, 1270, 1621
BstH2I RGCGCY 2 cut(s) 75, 2522
BstHHI GCGC 3 cut(s) 74, 248, 2521
BstMAI GTCTC 8 cut(s) 284, 311, 902, 976, 1257, 1967, 2917, 3590
BstMCI CGRYCG 3 cut(s) 455, 475, 1625
BstNI CCWGG 4 cut(s) 167, 1574, 2885, 3222
BstNSI RCATGY 2 cut(s) 2167, 2409
BstSFI CTRYAG 3 cut(s) 341, 3280, 3327
BstSLI GKGCMC 1 cut(s) 1401
BstUI CGCG 4 cut(s) 304, 499, 1270, 1621
BstV2I GAAGAC 2 cut(s) 78, 1184
BstX2I RGATCY 2 cut(s) 766, 1285
BstXI CCANNNNNNTGG 1 cut(s) 1460
BstYI RGATCY 2 cut(s) 766, 1285
Bsu36I CCTNAGG 1 cut(s) 67
BsuI GTATCC 1 cut(s) 2516
BsuRI GGCC 7 cut(s) 1433, 1860, 2382, 3140, 3325, 3499, 3511
BtgI CCRYGG 1 cut(s) 1619
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 6 cut(s) 1962, 2322, 2357, 3130, 3280, 3374
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 7 cut(s) 824, 1305, 1684, 2313, 2725, 2739, 3202
BtuMI TCGCGA 1 cut(s) 499
BveI ACCTGC 4 cut(s) 57, 239, 618, 2131
Cac8I GCNNGC 7 cut(s) 398, 1268, 1490, 1719, 1858, 2549, 3214
CaiI CAGNNNCTG 2 cut(s) 2610, 3466
CciI TCATGA 1 cut(s) 2974
CfoI GCGC 3 cut(s) 74, 248, 2521
Cfr10I RCCGGY 3 cut(s) 275, 1532, 1856
Cfr13I GGNCC 3 cut(s) 1238, 1463, 2143
Cfr42I CCGCGG 1 cut(s) 1622
Cfr9I CCCGGG 2 cut(s) 1871, 2254
CseI GACGC 3 cut(s) 293, 919, 2308
CsiI ACCWGGT 1 cut(s) 2883
Csp6I GTAC 7 cut(s) 697, 2124, 2393, 2484, 2902, 2941, 3241
CviQI GTAC 7 cut(s) 697, 2124, 2393, 2484, 2902, 2941, 3241
DinI GGCGCC 1 cut(s) 73
DraI TTTAAA 2 cut(s) 1806, 3541
DrdI GACNNNNNNGTC 1 cut(s) 248
DriI GACNNNNNGTC 1 cut(s) 3103
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 3389
Eam1105I GACNNNNNGTC 1 cut(s) 3103
EarI CTCTTC 1 cut(s) 3389
EciI GGCGGA 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 2397
Eco147I AGGCCT 1 cut(s) 3325
Eco31I GGTCTC 1 cut(s) 284
Eco32I GATATC 3 cut(s) 1069, 1208, 1687
Eco47I GGWCC 3 cut(s) 1238, 1463, 2143
Eco47III AGCGCT 1 cut(s) 2520
Eco57I CTGAAG 9 cut(s) 446, 530, 992, 1305, 1448, 1458, 2225, 3368, 3533
Eco81I CCTNAGG 1 cut(s) 67
Eco88I CYCGRG 5 cut(s) 935, 1102, 1750, 1871, 2254
EcoNI CCTNNNNNAGG 1 cut(s) 2331
EcoO109I RGGNCCY 1 cut(s) 2143
EcoRI GAATTC 1 cut(s) 2455
EcoRII CCWGG 4 cut(s) 165, 1572, 2883, 3220
EcoRV GATATC 3 cut(s) 1069, 1208, 1687
EcoT14I CCWWGG 1 cut(s) 2397
EcoT22I ATGCAT 1 cut(s) 136
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 2397
Esp3I CGTCTC 2 cut(s) 311, 902
FaqI GGGAC 5 cut(s) 1063, 1767, 1933, 2237, 2281
FbaI TGATCA 1 cut(s) 3490
FblI GTMKAC 3 cut(s) 2724, 2760, 3279
FokI GGATG 6 cut(s) 1949, 2309, 2344, 3137, 3287, 3381
GlaI GCGC 3 cut(s) 73, 247, 2520
GsaI CCCAGC 1 cut(s) 2357
GsuI CTGGAG 6 cut(s) 311, 1110, 1712, 2763, 2981, 3204
HaeII RGCGCY 2 cut(s) 75, 2522
HaeIII GGCC 7 cut(s) 1433, 1860, 2382, 3140, 3325, 3499, 3511
HgaI GACGC 3 cut(s) 293, 919, 2308
HhaI GCGC 3 cut(s) 74, 248, 2521
Hin1I GRCGYC 3 cut(s) 72, 2319, 3570
Hin6I GCGC 3 cut(s) 72, 246, 2519
HinP1I GCGC 3 cut(s) 72, 246, 2519
HincII GTYRAC 4 cut(s) 412, 591, 1633, 2013
HindII GTYRAC 4 cut(s) 412, 591, 1633, 2013
HindIII AAGCTT 3 cut(s) 1041, 1715, 2804
Hpy166II GTNNAC 9 cut(s) 412, 591, 1633, 1999, 2013, 2041, 2725, 2761, 3280
Hpy8I GTNNAC 9 cut(s) 412, 591, 1633, 1999, 2013, 2041, 2725, 2761, 3280
HpyCH4III ACNGT 9 cut(s) 409, 1180, 1826, 2038, 2491, 2714, 3095, 3284, 3601
HpyCH4IV ACGT 2 cut(s) 2649, 3570
HpySE526I ACGT 2 cut(s) 2649, 3570
Hsp92I GRCGYC 3 cut(s) 72, 2319, 3570
HspAI GCGC 3 cut(s) 72, 246, 2519
KasI GGCGCC 1 cut(s) 71
Kpn2I TCCGGA 3 cut(s) 1837, 2099, 2330
KpnI GGTACC 2 cut(s) 2396, 2487
KroI GCCGGC 1 cut(s) 1856
KroNI GCCGGC 1 cut(s) 1858
Ksp22I TGATCA 1 cut(s) 3490
KspI CCGCGG 1 cut(s) 1622
LmnI GCTCC 7 cut(s) 57, 121, 239, 1337, 1405, 1485, 1840
LweI GCATC 7 cut(s) 234, 1355, 1566, 1971, 2366, 2652, 3248
MabI ACCWGGT 1 cut(s) 2883
MaeII ACGT 2 cut(s) 2649, 3570
MaeIII GTNAC 5 cut(s) 250, 1145, 1656, 2815, 3095
MflI RGATCY 2 cut(s) 766, 1285
MhlI GDGCHC 3 cut(s) 1401, 2009, 3206
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 2 cut(s) 1045, 2223
MmeI TCCRAC 7 cut(s) 294, 447, 1872, 2191, 2325, 2615, 3346
Mph1103I ATGCAT 1 cut(s) 136
MroI TCCGGA 3 cut(s) 1837, 2099, 2330
MroNI GCCGGC 1 cut(s) 1856
MroXI GAANNNNTTC 3 cut(s) 1301, 1986, 2586
MseI TTAA 4 cut(s) 386, 1805, 2799, 3540
MslI CAYNNNNRTG 3 cut(s) 1458, 1643, 3195
MspA1I CMGCKG 5 cut(s) 1249, 1621, 1766, 2341, 2610
Mva1269I GAATGC 5 cut(s) 134, 583, 1303, 1898, 3056
MvaI CCWGG 4 cut(s) 167, 1574, 2885, 3222
MvnI CGCG 4 cut(s) 304, 499, 1270, 1621
NaeI GCCGGC 1 cut(s) 1858
NarI GGCGCC 1 cut(s) 72
NgoMIV GCCGGC 1 cut(s) 1856
NlaIV GGNNCC 7 cut(s) 73, 1612, 2104, 2394, 2485, 2882, 2889
NmuCI GTSAC 5 cut(s) 250, 1145, 1656, 2815, 3095
NruI TCGCGA 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 2 cut(s) 2167, 2409
NspV TTCGAA 3 cut(s) 575, 888, 1295
PaeR7I CTCGAG 2 cut(s) 935, 1102
PagI TCATGA 1 cut(s) 2974
PaqCI CACCTGC 2 cut(s) 57, 239
PasI CCCWGGG 1 cut(s) 166
PceI AGGCCT 1 cut(s) 3325
PctI GAATGC 5 cut(s) 134, 583, 1303, 1898, 3056
PdiI GCCGGC 1 cut(s) 1858
PdmI GAANNNNTTC 3 cut(s) 1301, 1986, 2586
PfeI GAWTC 9 cut(s) 9, 194, 653, 1585, 1705, 1759, 2432, 3520, 3557
PfoI TCCNGGA 3 cut(s) 710, 1388, 1883
PleI GAGTC 2 cut(s) 1045, 2222
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 2 cut(s) 1045, 2222
PpuMI RGGWCCY 1 cut(s) 2143
Psp5II RGGWCCY 1 cut(s) 2143
Psp6I CCWGG 4 cut(s) 165, 1572, 2883, 3220
PspFI CCCAGC 1 cut(s) 2353
PspGI CCWGG 4 cut(s) 165, 1572, 2883, 3220
PspN4I GGNNCC 7 cut(s) 73, 1612, 2104, 2394, 2485, 2882, 2889
PspPI GGNCC 3 cut(s) 1238, 1463, 2143
PspPPI RGGWCCY 1 cut(s) 2143
PspXI VCTCGAGB 1 cut(s) 1102
PstNI CAGNNNCTG 2 cut(s) 2610, 3466
PsuI RGATCY 2 cut(s) 766, 1285
PvuII CAGCTG 3 cut(s) 1766, 2341, 2610
RruI TCGCGA 1 cut(s) 499
RsaI GTAC 7 cut(s) 698, 2125, 2394, 2485, 2903, 2942, 3242
RsaNI GTAC 7 cut(s) 697, 2124, 2393, 2484, 2902, 2941, 3241
RseI CAYNNNNRTG 3 cut(s) 1458, 1643, 3195
SacII CCGCGG 1 cut(s) 1622
SaqAI TTAA 4 cut(s) 386, 1805, 2799, 3540
Sau96I GGNCC 3 cut(s) 1238, 1463, 2143
ScaI AGTACT 1 cut(s) 3242
SchI GAGTC 2 cut(s) 1045, 2223
SduI GDGCHC 3 cut(s) 1401, 2009, 3206
SexAI ACCWGGT 1 cut(s) 2883
SfaNI GCATC 7 cut(s) 234, 1355, 1566, 1971, 2366, 2652, 3248
SfcI CTRYAG 3 cut(s) 341, 3280, 3327
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 2 cut(s) 935, 1102
Sfr303I CCGCGG 1 cut(s) 1622
SfuI TTCGAA 3 cut(s) 575, 888, 1295
SgrAI CRCCGGYG 1 cut(s) 1532
SgrBI CCGCGG 1 cut(s) 1622
SinI GGWCC 3 cut(s) 1238, 1463, 2143
SlaI CTCGAG 2 cut(s) 935, 1102
SmaI CCCGGG 2 cut(s) 1873, 2256
SmiI ATTTAAAT 1 cut(s) 1806
SmiMI CAYNNNNRTG 3 cut(s) 1458, 1643, 3195
SmlI CTYRAG 6 cut(s) 755, 772, 935, 1055, 1102, 1201
SmoI CTYRAG 6 cut(s) 755, 772, 935, 1055, 1102, 1201
SpeI ACTAGT 1 cut(s) 1945
SseBI AGGCCT 1 cut(s) 3325
SspDI GGCGCC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 3325
StyI CCWWGG 1 cut(s) 2397
SwaI ATTTAAAT 1 cut(s) 1806
TaaI ACNGT 9 cut(s) 409, 1180, 1826, 2038, 2491, 2714, 3095, 3284, 3601
TaiI ACGT 2 cut(s) 2652, 3573
TatI WGTACW 2 cut(s) 2940, 3240
TauI GCSGC 8 cut(s) 96, 99, 102, 105, 108, 111, 1249, 2405
TfiI GAWTC 9 cut(s) 9, 194, 653, 1585, 1705, 1759, 2432, 3520, 3557
Tru1I TTAA 4 cut(s) 386, 1805, 2799, 3540
Tru9I TTAA 4 cut(s) 386, 1805, 2799, 3540
TscAI CASTG 7 cut(s) 824, 1312, 1684, 2320, 2725, 2746, 3202
TseFI GTSAC 5 cut(s) 250, 1145, 1656, 2815, 3095
Tsp45I GTSAC 5 cut(s) 250, 1145, 1656, 2815, 3095
TspDTI ATGAA 9 cut(s) 342, 721, 957, 1113, 1830, 2960, 2963, 3116, 3141
TspGWI ACGGA 2 cut(s) 12, 1578
TspMI CCCGGG 2 cut(s) 1871, 2254
TspRI CASTG 7 cut(s) 824, 1312, 1684, 2320, 2725, 2746, 3202
VpaK11BI GGWCC 3 cut(s) 1238, 1463, 2143
XagI CCTNNNNNAGG 1 cut(s) 2331
XapI RAATTY 8 cut(s) 502, 793, 945, 1166, 1479, 2019, 2455, 3130
XbaI TCTAGA 1 cut(s) 904
XceI RCATGY 2 cut(s) 2167, 2409
XhoI CTCGAG 2 cut(s) 935, 1102
XmaI CCCGGG 2 cut(s) 1871, 2254
XmiI GTMKAC 3 cut(s) 2724, 2760, 3279
XmnI GAANNNNTTC 3 cut(s) 1301, 1986, 2586
ZraI GACGTC 1 cut(s) 3571
ZrmI AGTACT 1 cut(s) 3242
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.