Rw5G045300
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002466
Physical Location & Seq
Forward (+)
79213813 .. 79217390
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045300.1

Sequence Viewer

Length: 3549 bp
ATGATGGCTTTGTTCTGTTTTCTGCTTCTGCTTCTTTTTCACTCTCTAAATTTCTCTTCAGCTGCTTCTAATGAGGATGTGGTGAAGAAGCTTTTGTTGCAGTTCAAGCAATCCTCTGTTCAATCTGACCCTCAAGGTTTCTTATCTGACTGGAAATCTGATTCTTCAACTGCTCCTTGTTCATGGAAAGGCCTGACTTGCTCTGAAGGCCACGTCATCACCCTCGACCTCTCCTCTCATGGTCTCATTGGAAGTCTTTACCTTCCAACTCTCACAGCCTTGCCAAGTCTTCAAAATCTGTACTTGCAAAACAATTCATTCTCTGCTACTGATCTTTCAGTTTCCAACATTACCTCATGTAGTCTTGTTACAGTTGACTTGTCCTCCAACAACATCACAAGCCCTCTTCCGGTTCGGTCTTTTCTTGAGGGTTGTGAGAATCTGGCTTTGGTCAACCTCTCTGGCAATTCAATCCCAGGAGGGAGTTTCAGCTTTGGTGCTTCCTTGCTTCAGCTTGACATCTCTAGAAACCTGATTTCGGATTCATCCCTGTTGACTTGCCAGAATCTGAATCTGCTCAATGTTTCCGGTAACAAGCTGACCGGAAAACTGAGTGATTCTATCTTGCCTTGCAAGAATCTGACGACACTTGACCTTTCATACAATGCTTTGTCTGGGGCGATACCAAACACTTTCCTGGAAAGCGTCTCGGCATCTCTCAAGTATTTGGATCTCTCAAGCAACAATTTCTCCGCAAAGTTTGCAAGCCTTGATTTCGGGCAGTGTAGCAGCCTCACTCTGCTGAAGCTATCACACAATAATCTCTATGGGGATGAGTTTCCTTCAAGCCTTGCTAACTGTCAAGCTTTGGAGACACTGAACCTGACTAGTAATAAGTTGCAGGACAAGATTCCGGGTGCTTTGTTGGGAAATCTCAAGAAGTTGAGGCAACTCTTTCTGGGTCGTAATCAGTTTTCAGGTGAAATTCCAGCTGAATTAGGAAAGGCTTGTGGGACACTCAAGGAGCTTGATATTTCCGATAACATTCTTACTGGTGAATTGCCTTCAAATTTTGTGTCATGCTCTTTTTTGGTTAGTCTCAATCTTGGTCGCAATCAGCTGTCAGGAAATTTCCTCAGTACTGTTGTGAGTAAGCTTCCGAGTTTGAGATATCTCTATGTACCCTTCAACAACATCACTGGTCCTGTGCCACCATCTATTACCAGTGGTACTCGGCTTCAAGTGCTCGATCTCAGCGCTAATAACTTCACAGGGAATGTTCCTTCAGTGTTTTGCTTCTCCAATGCTTCCTCAGCTTTGGAAAAGATACTTCTAGCCAACAATTTTCTCTCCGGGACTGTGCCATCGGAACTTGGAAACTGCAAGAATCTCAAGGCAATTGATCTTAGTTTCAACAGCTTGAGTGGTCCAATTCCTTCAGAAGTTTGGAGCTTGCCAAATCTTTCCGACTTGGTTATGTGGGCAAACAATCTCACTGGTGAAATCCCAGATGGCATTTGCATCAATGGAGGAAACCTGGAAACCCTGATTCTCAACAATAATCTCATTACTGGAGCCATTCCACAGTCAATTGCCAACTGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGGCTTACAGGAGCAATCCCTTCAGGTATTGGAAATCTCCTTAAGCTTGCTATACTTCAGTTAGGTAATAATTCACTGTCTGGTCAGATTCCAGCTGAGCTTGGCAAGTGTCAAAGCCTCATTTGGCTTGACCTGAACAGCAATGACCTAAATGGTTCTATCCCATCAGAGCTTGCTAACCAGGCCGGCCTAGTCATTCCTGGAACAGTTTCTGGGAAGCAGTTTGCATTTGTTAGAAATGAGGGTGTTGAGTTTGAGGGAGTACGACCTCAGAGACTAGAAAGTTTACCTATGGTTCATTCTTGTCCATCAACTAGAATTTACACAGGAATGGCAGTTTACACATTCACCAGCAATGGAAGCATGATCTTCCTTGACGTTTCCTACAATTACTTGTCTGGGACTATTCCCGCAAACTTAGGTACACTGTCCTATTTGCAAGTCTTCAATCTAGGACACAATATGTTAAGTGGAAATATTCCAGAAAGCTTTGGAGGATTAAAAGCAATTGGCGTCCTGGATCTCTCTCACAATAATCTTCAAGGTTATGTCCCAGGATCTTTGGGGAATCTCTCTTTCCTGAGTGACCTTGATGTGTCTAACAACAACCTCACTGGCCCTGTCCCTTCCGGAGGTCAGCTGACAACTTTCCCAGCATCCAGATATGAGAACAACTCTGGCCTTTGTGGGTTACCCCTGCCACCGTGTGGCTCTCAAAGACATTCAGCAGAGAGAGTTAGGGGAAAGAAGCCATCTATGGCTTCAGGAATGGTTATTGGCATCGCATTCTTTCTCTTCTGTATCCTTATACTTGCATTAGCTCTATATCGAGTGAAGAAGTACCAGCGGAAAGAGGCGAAGAGAGAAAAATATATTGAAAGCCTTCCGACTTCAGGTAGCAGCAGCTGGAAACTTTCCAGTGTTCCTGAGCCTCTCAGCATCAACGTAGCCACATTTGAAAAACCTTTGAGGAAGCTGACCTTCGCCCATTTACTTGAAGCCACCAATGGTTTCAGTGCTGATAGCTTGATTGGATCTGGAGGGTTTGGTGAGGTCTACAAGGCACAATTGGGAGATGGCTGTGTTGTTGCAATCAAGAAGCTTATTCAAGTCACAGGTCAAGGAGACCGAGAGTTTATGGCAGAAATGGAAACTATTGGGAAAATCAAACACCGAAACCTGGTTCCTTTGCTGGGGTACTGCAAGGTTGGAGAGGAGAGACTTCTTGTGTATGAGTACATGAAATGGGGAAGTCTGGAGAGTGTTTTTCATGATAAGATTAAGGGAGGGGGCTCCAGGCTTGACTGGGCAGCACGAAAGAAGATTGCCATAGGGTCTGCTAGAGGTCTAGCATTCCTTCACCATAGCTGCATACCTCATATTATCCACCGGGACATGAAGTCTAGCAATGTTCTTATAGATGAAAACTTTGAGGCCAGAGTATCAGATTTTGGCATGGCAAGATTGGTCAATGCCCTCGATACTCATCTCAGTGTGAGCACCCTAGCAGGAACTCCAGGTTACGTGCCCCCCGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGAGATGTCTACAGTTATGGTGTTATACTGCTAGAGCTTCTCTCAGGAAAAAGGCCTATAGACCCGTCAGCATTTGGTGATGACAACAACCTTGTTGGATGGGCAAAGCAGCTTCAAAGAGAAAAGAGATGGAATGAGATACTCGACACTGAGTTGATGACACAAAAATCTGGTGAGGCTGAACTATACCAGTATCTGAAGATTGCCTTTGAGTGCCTAGACGACAGGCCATTCCGGCGGCCAACAATGATTCAGGTTATGGCTATGTTTAAAGAGCTTCAGGTTGATTCGGACAGTGATGTCCTGGATGGTTTTTCACTCAAAGACACTGTTGCTGAGGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1182

Amino Acids

127.92

Weight (kDa)

6.05

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 68 2.9e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 339 - 399 5.1e-06 Leucine rich repeat
Island PF20141 608 - 662 2.2e-18 Brassinosteroid receptor island domain
LRR_8 PF13855 670 - 725 1.8e-07 Leucine rich repeat
LRR_8 PF13855 691 - 749 1.6e-07 Leucine rich repeat
Pkinase PF00069 885 - 1155 8.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 886 - 1155 1.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3500
AccB7I CCANNNNNTGG 1 cut(s) 2342
AccI GTMKAC 2 cut(s) 2691, 3210
AccIII TCCGGA 1 cut(s) 2264
AciI CCGC 4 cut(s) 753, 2046, 2482, 3439
AclWI GGATC 4 cut(s) 738, 2163, 2200, 2677
AcoI YGGCCR 1 cut(s) 3440
AcsI RAATTY 5 cut(s) 49, 984, 1069, 1129, 1953
AcyI GRCGYC 1 cut(s) 2148
AdeI CACNNNGTG 1 cut(s) 2342
AfeI AGCGCT 1 cut(s) 1258
AfiI CCNNNNNNNGG 7 cut(s) 409, 538, 2267, 2342, 2528, 2815, 2828
AflII CTTAAG 1 cut(s) 1676
AhdI GACNNNNNGTC 1 cut(s) 3034
AhlI ACTAGT 1 cut(s) 887
AjiI CACGTC 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
Alw21I GWGCWC 2 cut(s) 1248, 3137
Alw26I GTCTC 7 cut(s) 248, 712, 866, 1103, 1903, 2754, 2848
AlwI GGATC 4 cut(s) 738, 2163, 2200, 2677
AlwNI CAGNNNCTG 4 cut(s) 568, 1847, 2541, 3397
Ama87I CYCGRG 1 cut(s) 3167
Aor13HI TCCGGA 1 cut(s) 2264
Aor51HI AGCGCT 1 cut(s) 1258
ApeKI GCWGC 7 cut(s) 62, 789, 2535, 2538, 2945, 3003, 3310
ApoI RAATTY 5 cut(s) 49, 984, 1069, 1129, 1953
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
Asp700I GAANNNNTTC 2 cut(s) 1843, 2517
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 3 cut(s) 1202, 1427, 2252
AsuC2I CCSGG 3 cut(s) 915, 1354, 3026
AvaI CYCGRG 1 cut(s) 3167
AvaII GGWCC 2 cut(s) 1202, 1427
BaeGI GKGCMC 1 cut(s) 3165
BanII GRGCYC 1 cut(s) 2930
BbsI GAAGAC 2 cut(s) 281, 2071
Bbv12I GWGCWC 2 cut(s) 1248, 3137
BbvCI CCTCAGC 2 cut(s) 1312, 3537
BbvI GCAGC 7 cut(s) 49, 801, 2547, 2550, 2957, 2990, 3322
BciVI GTATCC 1 cut(s) 2447
BcnI CCSGG 3 cut(s) 915, 1354, 3026
BcoDI GTCTC 7 cut(s) 248, 712, 866, 1103, 1903, 2754, 2848
BcuI ACTAGT 1 cut(s) 887
BfmI CTRYAG 2 cut(s) 3211, 3258
BfoI RGCGCY 1 cut(s) 1260
BfrI CTTAAG 1 cut(s) 1676
BfuI GTATCC 1 cut(s) 2447
BglI GCCNNNNNGGC 1 cut(s) 3436
BisI GCNGC 8 cut(s) 63, 790, 2536, 2539, 2946, 3004, 3311, 3440
BlpI GCTNAGC 1 cut(s) 1731
BlsI GCNGC 8 cut(s) 64, 791, 2537, 2540, 2947, 3005, 3312, 3441
BmcAI AGTACT 2 cut(s) 1141, 3173
Bme18I GGWCC 2 cut(s) 1202, 1427
BmeRI GACNNNNNGTC 1 cut(s) 3034
BmeT110I CYCGRG 1 cut(s) 3167
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 3 cut(s) 1202, 1427, 2252
BmiI GGNNCC 4 cut(s) 1576, 2820, 2929, 3543
BmrI ACTGGG 1 cut(s) 2950
BmsI GCATC 6 cut(s) 722, 1530, 2300, 2424, 2583, 3179
BmuI ACTGGG 1 cut(s) 2950
BpiI GAAGAC 2 cut(s) 281, 2071
BplI GAGNNNNNCTC 4 cut(s) 2294, 2326, 3227, 3259
BpmI CTGGAG 5 cut(s) 1593, 2694, 2912, 2914, 3135
Bpu10I CCTNAGC 3 cut(s) 1312, 2562, 3537
Bpu1102I GCTNAGC 1 cut(s) 1731
BpuEI CTTGAG 8 cut(s) 117, 446, 704, 721, 920, 1004, 1376, 1441
BpuMI CCSGG 3 cut(s) 915, 1354, 3026
BsaAI YACGTR 1 cut(s) 3160
BsaBI GATNNNNATC 1 cut(s) 3120
BsaHI GRCGYC 1 cut(s) 2148
BsaI GGTCTC 2 cut(s) 248, 2754
BsaJI CCNNGG 2 cut(s) 475, 2188
BsaWI WCCGGW 4 cut(s) 409, 587, 602, 2264
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 734, 764
Bsc4I CCNNNNNNNGG 7 cut(s) 409, 538, 2267, 2342, 2528, 2815, 2828
Bse118I RCCGGY 2 cut(s) 1635, 1820
Bse3DI GCAATG 3 cut(s) 1783, 1996, 3049
Bse8I GATNNNNATC 1 cut(s) 3120
BseAI TCCGGA 1 cut(s) 2264
BseDI CCNNGG 2 cut(s) 475, 2188
BseGI GGATG 6 cut(s) 82, 545, 838, 2291, 3305, 3514
BseJI GATNNNNATC 1 cut(s) 3120
BseLI CCNNNNNNNGG 7 cut(s) 409, 538, 2267, 2342, 2528, 2815, 2828
BseMI GCAATG 3 cut(s) 1783, 1996, 3049
BseRI GAGGAG 2 cut(s) 223, 2864
BseSI GKGCMC 1 cut(s) 3165
BseXI GCAGC 7 cut(s) 49, 801, 2547, 2550, 2957, 2990, 3322
BseYI CCCAGC 2 cut(s) 2287, 2827
BsgI GTGCAG 1 cut(s) 1585
BsiHKAI GWGCWC 2 cut(s) 1248, 3137
BsiHKCI CYCGRG 1 cut(s) 3167
BslFI GGGAC 6 cut(s) 1027, 1369, 2050, 2171, 2243, 3041
BslI CCNNNNNNNGG 7 cut(s) 409, 538, 2267, 2342, 2528, 2815, 2828
BsmAI GTCTC 7 cut(s) 248, 712, 866, 1103, 1903, 2754, 2848
BsmBI CGTCTC 1 cut(s) 712
BsmFI GGGAC 6 cut(s) 1027, 1369, 2050, 2171, 2243, 3041
BsmI GAATGC 2 cut(s) 2420, 2987
Bso31I GGTCTC 2 cut(s) 248, 2754
BsoBI CYCGRG 1 cut(s) 3167
Bsp1286I GDGCHC 4 cut(s) 1248, 2930, 3137, 3165
Bsp13I TCCGGA 1 cut(s) 2264
Bsp143I GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
Bsp1720I GCTNAGC 1 cut(s) 1731
BspACI CCGC 4 cut(s) 753, 2046, 2482, 3439
BspEI TCCGGA 1 cut(s) 2264
BspHI TCATGA 1 cut(s) 2905
BspLI GGNNCC 4 cut(s) 1576, 2820, 2929, 3543
BspPI GGATC 4 cut(s) 738, 2163, 2200, 2677
BspTI CTTAAG 1 cut(s) 1676
BspTNI GGTCTC 2 cut(s) 248, 2754
BsrDI GCAATG 3 cut(s) 1783, 1996, 3049
BsrFI RCCGGY 2 cut(s) 1635, 1820
BssAI RCCGGY 2 cut(s) 1635, 1820
BssECI CCNNGG 2 cut(s) 475, 2188
BssMI GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
BssNI GRCGYC 1 cut(s) 2148
Bst6I CTCTTC 4 cut(s) 61, 411, 2435, 2489
BstACI GRCGYC 1 cut(s) 2148
BstAFI CTTAAG 1 cut(s) 1676
BstAPI GCANNNNNTGC 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 3160
BstC8I GCNNGC 5 cut(s) 766, 1454, 1683, 1809, 1822
BstEII GGTNACC 1 cut(s) 2325
BstENI CCTNNNNNAGG 1 cut(s) 2265
BstF5I GGATG 6 cut(s) 82, 545, 838, 2291, 3305, 3514
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 8 cut(s) 334, 733, 1252, 1405, 2004, 2158, 2195, 2672
BstMAI GTCTC 7 cut(s) 248, 712, 866, 1103, 1903, 2754, 2848
BstMBI GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
BstPI GGTNACC 1 cut(s) 2325
BstSFI CTRYAG 2 cut(s) 3211, 3258
BstSLI GKGCMC 1 cut(s) 3165
BstV1I GCAGC 7 cut(s) 49, 801, 2547, 2550, 2957, 2990, 3322
BstV2I GAAGAC 2 cut(s) 281, 2071
BstX2I RGATCY 4 cut(s) 730, 2155, 2192, 2669
BstYI RGATCY 4 cut(s) 730, 2155, 2192, 2669
BsuI GTATCC 1 cut(s) 2447
BtgZI GCGATG 1 cut(s) 2401
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 6 cut(s) 82, 545, 838, 2291, 3305, 3514
BtsI GCAGTG 1 cut(s) 788
Cac8I GCNNGC 5 cut(s) 766, 1454, 1683, 1809, 1822
CaiI CAGNNNCTG 4 cut(s) 568, 1847, 2541, 3397
CciI TCATGA 1 cut(s) 2905
CfoI GCGC 1 cut(s) 1259
Cfr10I RCCGGY 2 cut(s) 1635, 1820
Cfr13I GGNCC 3 cut(s) 1202, 1427, 2252
CseI GACGC 2 cut(s) 694, 2137
CsiI ACCWGGT 1 cut(s) 2814
DpnI GATC 8 cut(s) 333, 732, 1251, 1404, 2003, 2157, 2194, 2671
DpnII GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
DraI TTTAAA 1 cut(s) 3472
DraIII CACNNNGTG 1 cut(s) 2342
DrdI GACNNNNNNGTC 1 cut(s) 3500
DriI GACNNNNNGTC 1 cut(s) 3034
DseDI GACNNNNNNGTC 1 cut(s) 3500
EaeI YGGCCR 1 cut(s) 3440
Eam1104I CTCTTC 4 cut(s) 61, 411, 2435, 2489
Eam1105I GACNNNNNGTC 1 cut(s) 3034
EarI CTCTTC 4 cut(s) 61, 411, 2435, 2489
Eco147I AGGCCT 2 cut(s) 192, 3256
Eco24I GRGCYC 1 cut(s) 2930
Eco31I GGTCTC 2 cut(s) 248, 2754
Eco32I GATATC 1 cut(s) 1172
Eco47I GGWCC 2 cut(s) 1202, 1427
Eco47III AGCGCT 1 cut(s) 1258
Eco88I CYCGRG 1 cut(s) 3167
Eco91I GGTNACC 1 cut(s) 2325
EcoNI CCTNNNNNAGG 1 cut(s) 2265
EcoO65I GGTNACC 1 cut(s) 2325
EcoRV GATATC 1 cut(s) 1172
EcoT38I GRGCYC 1 cut(s) 2930
Esp3I CGTCTC 1 cut(s) 712
FalI AAGNNNNNCTT 4 cut(s) 2600, 2632, 3392, 3424
FaqI GGGAC 6 cut(s) 1027, 1369, 2050, 2171, 2243, 3041
FauI CCCGC 1 cut(s) 2053
FblI GTMKAC 2 cut(s) 2691, 3210
Fnu4HI GCNGC 8 cut(s) 63, 790, 2536, 2539, 2946, 3004, 3311, 3440
FokI GGATG 6 cut(s) 89, 532, 845, 2278, 3312, 3521
FriOI GRGCYC 1 cut(s) 2930
FseI GGCCGGCC 1 cut(s) 1824
Fsp4HI GCNGC 8 cut(s) 63, 790, 2536, 2539, 2946, 3004, 3311, 3440
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 8 cut(s) 63, 790, 2536, 2539, 2946, 3004, 3311, 3440
GsaI CCCAGC 2 cut(s) 2291, 2831
GsuI CTGGAG 5 cut(s) 1593, 2694, 2912, 2914, 3135
HaeII RGCGCY 1 cut(s) 1260
HgaI GACGC 2 cut(s) 694, 2137
HhaI GCGC 1 cut(s) 1259
Hin1I GRCGYC 1 cut(s) 2148
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HincII GTYRAC 3 cut(s) 376, 454, 555
HindII GTYRAC 3 cut(s) 376, 454, 555
HindIII AAGCTT 6 cut(s) 89, 864, 1154, 1679, 2122, 2735
Hpy166II GTNNAC 8 cut(s) 376, 454, 555, 1922, 1975, 2060, 2692, 3211
Hpy8I GTNNAC 8 cut(s) 376, 454, 555, 1922, 1975, 2060, 2692, 3211
HpyCH4IV ACGT 4 cut(s) 213, 2013, 2580, 3159
HpySE526I ACGT 4 cut(s) 213, 2013, 2580, 3159
Hsp92I GRCGYC 1 cut(s) 2148
HspAI GCGC 1 cut(s) 1257
Kpn2I TCCGGA 1 cut(s) 2264
KroI GCCGGC 1 cut(s) 1820
KroNI GCCGGC 1 cut(s) 1822
Kzo9I GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
LmnI GCTCC 6 cut(s) 178, 1024, 1449, 1574, 1646, 2933
Lsp1109I GCAGC 7 cut(s) 49, 801, 2547, 2550, 2957, 2990, 3322
LweI GCATC 6 cut(s) 722, 1530, 2300, 2424, 2583, 3179
MabI ACCWGGT 1 cut(s) 2814
MaeII ACGT 4 cut(s) 213, 2013, 2580, 3159
MaeIII GTNAC 7 cut(s) 367, 590, 1620, 2219, 2325, 2746, 3155
MalI GATC 8 cut(s) 333, 732, 1251, 1404, 2003, 2157, 2194, 2671
MboI GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
MfeI CAATTG 4 cut(s) 1398, 1590, 2142, 2702
MflI RGATCY 4 cut(s) 730, 2155, 2192, 2669
MhlI GDGCHC 4 cut(s) 1248, 2930, 3137, 3165
MmeI TCCRAC 7 cut(s) 290, 369, 411, 1491, 2546, 2824, 3277
MroI TCCGGA 1 cut(s) 2264
MroNI GCCGGC 1 cut(s) 1820
MroXI GAANNNNTTC 2 cut(s) 1843, 2517
MseI TTAA 5 cut(s) 1677, 2102, 2135, 2916, 3471
MslI CAYNNNNRTG 3 cut(s) 1607, 1964, 3126
MspA1I CMGCKG 7 cut(s) 62, 992, 1120, 1730, 2275, 2482, 2541
MspCI CTTAAG 1 cut(s) 1676
MunI CAATTG 4 cut(s) 1398, 1590, 2142, 2702
Mva1269I GAATGC 2 cut(s) 2420, 2987
NaeI GCCGGC 1 cut(s) 1822
NciI CCSGG 3 cut(s) 915, 1354, 3026
NdeII GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
NgoMIV GCCGGC 1 cut(s) 1820
NlaIV GGNNCC 4 cut(s) 1576, 2820, 2929, 3543
NmeAIII GCCGAG 2 cut(s) 689, 1213
NmuCI GTSAC 3 cut(s) 1620, 2219, 2746
PagI TCATGA 1 cut(s) 2905
PceI AGGCCT 2 cut(s) 192, 3256
PcsI WCGNNNNNNNCGW 1 cut(s) 3165
PctI GAATGC 2 cut(s) 2420, 2987
PdiI GCCGGC 1 cut(s) 1822
PdmI GAANNNNTTC 2 cut(s) 1843, 2517
PflMI CCANNNNNTGG 1 cut(s) 2342
PfoI TCCNGGA 5 cut(s) 696, 1352, 1834, 2151, 3504
PkrI GCNGC 8 cut(s) 64, 791, 2537, 2540, 2947, 3005, 3312, 3441
Ppu21I YACGTR 1 cut(s) 3160
PspEI GGTNACC 1 cut(s) 2325
PspFI CCCAGC 2 cut(s) 2287, 2827
PspN4I GGNNCC 4 cut(s) 1576, 2820, 2929, 3543
PspPI GGNCC 3 cut(s) 1202, 1427, 2252
PstNI CAGNNNCTG 4 cut(s) 568, 1847, 2541, 3397
PsuI RGATCY 4 cut(s) 730, 2155, 2192, 2669
PvuII CAGCTG 6 cut(s) 62, 992, 1120, 1730, 2275, 2541
RigI GGCCGGCC 1 cut(s) 1824
RseI CAYNNNNRTG 3 cut(s) 1607, 1964, 3126
SaqAI TTAA 5 cut(s) 1677, 2102, 2135, 2916, 3471
SatI GCNGC 8 cut(s) 63, 790, 2536, 2539, 2946, 3004, 3311, 3440
Sau3AI GATC 8 cut(s) 331, 730, 1249, 1402, 2001, 2155, 2192, 2669
Sau96I GGNCC 3 cut(s) 1202, 1427, 2252
ScaI AGTACT 2 cut(s) 1141, 3173
SduI GDGCHC 4 cut(s) 1248, 2930, 3137, 3165
SexAI ACCWGGT 1 cut(s) 2814
SfaNI GCATC 6 cut(s) 722, 1530, 2300, 2424, 2583, 3179
SfcI CTRYAG 2 cut(s) 3211, 3258
SinI GGWCC 2 cut(s) 1202, 1427
SmiMI CAYNNNNRTG 3 cut(s) 1607, 1964, 3126
SmlI CTYRAG 9 cut(s) 132, 425, 719, 736, 935, 1019, 1391, 1420, 1676
SmoI CTYRAG 9 cut(s) 132, 425, 719, 736, 935, 1019, 1391, 1420, 1676
SpeI ACTAGT 1 cut(s) 887
SseBI AGGCCT 2 cut(s) 192, 3256
SsiI CCGC 4 cut(s) 753, 2046, 2482, 3439
SspI AATATT 1 cut(s) 2113
StuI AGGCCT 2 cut(s) 192, 3256
TaiI ACGT 4 cut(s) 216, 2016, 2583, 3162
TaqI TCGA 6 cut(s) 225, 1248, 2464, 3114, 3187, 3345
TaqII GACCGA 2 cut(s) 405, 2778
TatI WGTACW 4 cut(s) 300, 1139, 2871, 3171
TauI GCSGC 1 cut(s) 3442
Tru1I TTAA 5 cut(s) 1677, 2102, 2135, 2916, 3471
Tru9I TTAA 5 cut(s) 1677, 2102, 2135, 2916, 3471
TseFI GTSAC 3 cut(s) 1620, 2219, 2746
TseI GCWGC 7 cut(s) 62, 789, 2535, 2538, 2945, 3003, 3310
Tsp45I GTSAC 3 cut(s) 1620, 2219, 2746
TspDTI ATGAA 9 cut(s) 171, 306, 534, 648, 1922, 2891, 2894, 3047, 3072
Van91I CCANNNNNTGG 1 cut(s) 2342
Vha464I CTTAAG 1 cut(s) 1676
VpaK11BI GGWCC 2 cut(s) 1202, 1427
XagI CCTNNNNNAGG 1 cut(s) 2265
XapI RAATTY 5 cut(s) 49, 984, 1069, 1129, 1953
XbaI TCTAGA 1 cut(s) 524
XmiI GTMKAC 2 cut(s) 2691, 3210
XmnI GAANNNNTTC 2 cut(s) 1843, 2517
ZrmI AGTACT 2 cut(s) 1141, 3173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.