RLG00000036472
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002466
Physical Location & Seq
Forward (+)
81047704 .. 81051279
3576 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036472

Sequence Viewer

Length: 3576 bp
ATGGGTTTGTTCTGTTTTCTGGTTCTGCTTCTTTTTCACTCTCTAAATTTCACTTCAGCTGCTTCTAATGAGGATGTGGTGAAGAAGCTTTTGTTGCAGTTCAAGCAATCCTCTGTTCAATCTGACCCTCATGGTTTCTTATCTGACTGGAACTCTGATTCTTCAACTGCCCTTTGTTCATGGAAAGGCCTGACTTGCTCTGAAGGCCACGTCATCACCCTCGACCTCTCCTATTCTGGTCTCATTGGAAGTCTTCACCTTCCAACTCTCACAGCCTTGCCAAGTCTTCAAAATCTGTACTTGCAAGGCAATTCATTCTCTGCTTCTGATCTTTCAGTTTCCAACATTACCTCATGTAGTCTTGTTATAGTTGACTTGTCCTCCAACAACATCACAAGCCCTCTTCCGGTTCGGTCTTTTCTTGAGGGTTGTGAGAATCTGGCTTTGGTCAACCTCTCTGGCAATTCAATCCCAGGAGGGAGTTTCAGCTTTGTTGCTTCCTTGCTTCAGCTTGACATCTCTAGAAACCTGATTTCGGATTCATCCTTTTTGACTTGCCAGAATCTGAATCTGCTCAATGTTTCTGGTAACAAGCTGACCGGAAAACTGAGTGGTTCTATCTTGACTTGCAAGAATCTGACGACATTTGACCTTTCACACAATGCTTTGTCTGGGGAGATACCAAACACTTTCCTGGAAAGCGTCTCGGCATCTCTCAAGTATTTGGATCTCTCAAGCAACAATTTCTCTGCAAAGTTTGCAAGCCTTGATTTCGGGCAGTGTAGCAGCCTCACTTTGCTGAAGCTATCACACAATAATCTCTATGGGGATGAGTTTCCTTCAAGCCTTGCTAACTGTCAAGCTTTGGAGACACTGAACCTGACTAGTAATAAGTTGCAGGACAAGATTCCGGGTGCTTTGTTGGGAAATCTCAAGAAGTTGAGGCAACTCTTTCTGGGTCGTAATCAGTTTTCAGGTGAAATTCCTGCTGAATTAGGAAAGGCTTGTGGGACACTTCAGGAGCTTGATATTTCCGATAACATTCTTACTGGTGAATTGCCTTCCAATTTTGTGTCATGCTCTTCTTTGGTTAGTCTCAATCTTGGTCGCAATCAGCTCTCAGGAAATTTCCTCAGTACTGTTGTGAGTAAGCTTCCGAGTTTGAGATATCTCTATGTACCCTTCAACAACATCACTGGTCCTGTGCCACCATCTATTACCAATGGTACTCGGCTTCAAGTGCTAGATCTCAGCGCTAATCTCTTCACAGGGAATGTTCCTTCAGTGTTTTGCTTCCCCAATGCTCCCTCAGCTTTGGAAAAGATACTTCTAGCCAACAATTTTCTCTCCGGGGCTGTGCCATCAGAACTTGGAAACTGCAAGAACCTCAAGGCAATTGATCTTAGTTTCAACCGCTTGAGTGGTCCAATTCCTTCGGAAGTTTGGAGCTTGCCAAATCTTTCCGACTTGGTTATGTGGGCAAACAATCTCACTGGTGAAATCCCACAAGGCATTTGCATCAATGGAGGAAACCTGGAAACCCTGATTCTCAACAATAATCTCATTACTGGAGCCATTCCACAGTCGATTGCCAACTGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGGCTTACAGGAGCAATCCCTTCAGGTATTGGAAATCTCCTTAAGCTTGCTATACTTCAGTTGGGTAATAATTCACTGTCGGGTCAGATTCCAGCTGAGCTTGGCAAGTGTCAAAGCCTTATTTGGCTTGACCTGAACAGCAATGACCTAAATGGTTCTATCCCATCAGAGCTTGCTAACCAGGCCAGCCTAGTCATTCCTGGAACAGTTTCTGGGAAGCAGTTTGCATTTGTTAGAAATGAGGGTGGTACAGCTTGCAGGGGTGCAGGAGGACTGGTTGAGTTTGAGGGAGTCCGACCTCAGAGACTCGAAGGTTTACCTATGGTTCATTCTTGTCCATCAACTAGAATTTACACAGGAATGACAGTTTACACTTTCACCAGCAATGGAAGCATGATCTTCCTTGACATTTCCTACAATCACTTGTCAGGGACTATTCCCGCAAACTTAGGTACACTGTCCTATTTGCAAGTCTTCAATCTAGGACACAATATGTTAACTGGAAATATTCCAGAAAGCTTTGGAGGATTAAAAGCAATTGGAGTCCTCGATCTCTCTCACAATAATCTTCAAGGTTATGTCCCAGGATCTTTGGGGACTCTCTCTTTCCTGAGTGACCTTGATGTGTCTAACAACAACCTCACGGGCCCTGTCCCTTCCGGAGGTCAGCTGACGACTTTCCCAGCATCCAGATATGAGAACAACTCTGGCCTTTGTGGGTTACCCCTGCCACCGTGTGACCCTCAAAGACATTCAGCAGAGAGAGTTAGGGGAAAGAAGCCATCTATGGCCTCAGGAATGGTTATTGGCATCGCATTCTTTCTCTTCTGTATCCTTATACTTGCATTAGCTCTATATCGAGTGAAGAAGTACCAGCGGAAAGAGGCTAAGAGAGAAAAATATATTGAAAGCCTTCCAACTTCAGGTAGCAGCAGCTGGAAACTTTCCAGTGTTCCTGAGCCTCTCAGCATCAACGTAGCCACATTTGAAAAACCTTTGAGGAAGCTGACCTTCGCCCATTTACTTGAAGCCACCAATGGTTTCAGTGCTGATAGCTTGATTGGTTCTGGAGGGTTTGGTGAGGTGTACAAGGCACAATTGGGAGATGGCTGTGTTGTTGCAATCAAGAAGCTTATTCAAGTCACAGGTCAAGGAGACCGAGAGTTTATGGCAGAAATGGAAACTATTGGGAAAATCAAACACCGAAACCTGGTTCCTTTGCTGGGGTACTGCAAGGTTGGAGAGGAGAGACTTCTTGTGTATGAGTACATGAAATGGGGAAGTCTGGAGAGTGTTTTTCATGATAAGATTAAGGGAGGGGGCTCAAGGCTTGACTGGGCAGCACGAAAGAAGATTGCCATAGGGTCTGCTAGAGGTCTAGCATTCCTTCACCATAGCTGCATACCTCATATTATCCACCGGGACATGAAGTCTAGCAATGTTCTTATAGATGAAAACTTTGAGGCCAGAGTATCAGATTTTGGCATGGCAAGATTGGTCAATGCCCTCGATACTCATCTCAGTGTGAGCACCCTAGCAGGAACTCCAGGTTACGTGCCCCCCGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGAGATGTCTACAGTTATGGTGTTATACTGCTAGAGCTTCTCTCAGGAAAAAGGCCTATAGACCCGTCAGCATTTGGTGATGACAACAACCTTGTTGGATGGGCAAAGCAGCTTCAAAGAGAAAAGAGATGGAATGAGATACTCGACACTGAGTTGATGACACAAAAATCTGGTGAGGCTGAACTATACCAGTATCTGAAGATTGCCTTTGAGTGCCTAGACGACAGGCCATTCCGGCGGCCAACAATGATTCAGGTTATGGCCATGTTTAAAGAGCTTCAGGTTGATTCGGACAGTGATGTCCTGGATGGTTTTTCACTCAAAGACACTGTTGCTGAGGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1192

Amino Acids

128.78

Weight (kDa)

6.14

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 67 5.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 281 - 391 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 338 - 398 3.5e-07 Leucine rich repeat
Island PF20141 608 - 671 1.6e-30 Brassinosteroid receptor island domain
LRR_8 PF13855 679 - 734 7.3e-08 Leucine rich repeat
LRR_8 PF13855 699 - 758 7.6e-08 Leucine rich repeat
Pkinase PF00069 894 - 1164 8.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 895 - 1164 1.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3527
AccI GTMKAC 1 cut(s) 3237
AccIII TCCGGA 1 cut(s) 2291
AciI CCGC 4 cut(s) 1414, 2073, 2509, 3466
AclWI GGATC 2 cut(s) 735, 2227
AcoI YGGCCR 2 cut(s) 3467, 3489
AcsI RAATTY 4 cut(s) 46, 981, 1126, 1980
AdeI CACNNNGTG 1 cut(s) 2369
AfeI AGCGCT 1 cut(s) 1255
AfiI CCNNNNNNNGG 6 cut(s) 406, 535, 2294, 2555, 2842, 2855
AflII CTTAAG 1 cut(s) 1673
AhdI GACNNNNNGTC 1 cut(s) 3061
AhlI ACTAGT 1 cut(s) 884
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 9 cut(s) 472, 693, 1533, 1812, 1831, 2215, 2841, 3178, 3531
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
Alw21I GWGCWC 1 cut(s) 3164
Alw26I GTCTC 7 cut(s) 245, 709, 863, 1100, 1930, 2781, 2875
AlwI GGATC 2 cut(s) 735, 2227
AlwNI CAGNNNCTG 4 cut(s) 565, 1844, 2568, 3424
Ama87I CYCGRG 1 cut(s) 3194
Aor13HI TCCGGA 1 cut(s) 2291
Aor51HI AGCGCT 1 cut(s) 1255
ApaI GGGCCC 1 cut(s) 2282
ApeKI GCWGC 7 cut(s) 59, 786, 2562, 2565, 2972, 3030, 3337
ApoI RAATTY 4 cut(s) 46, 981, 1126, 1980
Asp700I GAANNNNTTC 2 cut(s) 1840, 2544
AspLEI GCGC 1 cut(s) 1256
AspS9I GGNCC 4 cut(s) 1199, 1424, 2278, 2279
AsuC2I CCSGG 3 cut(s) 912, 1351, 3053
AvaI CYCGRG 1 cut(s) 3194
AvaII GGWCC 2 cut(s) 1199, 1424
AxyI CCTNAGG 1 cut(s) 2425
BaeGI GKGCMC 2 cut(s) 2282, 3192
BalI TGGCCA 1 cut(s) 3491
BanII GRGCYC 2 cut(s) 2282, 2957
BbsI GAAGAC 3 cut(s) 245, 278, 2098
Bbv12I GWGCWC 1 cut(s) 3164
BbvCI CCTCAGC 2 cut(s) 1309, 3564
BbvI GCAGC 7 cut(s) 46, 798, 2574, 2577, 2984, 3017, 3349
BccI CCATC 9 cut(s) 1219, 1369, 1804, 1978, 2422, 2732, 3321, 3351, 3530
BciT130I CCWGG 9 cut(s) 474, 695, 1535, 1814, 1833, 2217, 2843, 3180, 3533
BciVI GTATCC 1 cut(s) 2474
BcnI CCSGG 3 cut(s) 912, 1351, 3053
BcoDI GTCTC 7 cut(s) 245, 709, 863, 1100, 1930, 2781, 2875
BcuI ACTAGT 1 cut(s) 884
BfmI CTRYAG 2 cut(s) 3238, 3285
BfoI RGCGCY 1 cut(s) 1257
BfrI CTTAAG 1 cut(s) 1673
BfuI GTATCC 1 cut(s) 2474
BglI GCCNNNNNGGC 1 cut(s) 3463
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 8 cut(s) 60, 787, 2563, 2566, 2973, 3031, 3338, 3467
BlpI GCTNAGC 1 cut(s) 1728
BlsI GCNGC 8 cut(s) 61, 788, 2564, 2567, 2974, 3032, 3339, 3468
BmcAI AGTACT 2 cut(s) 1138, 3200
Bme18I GGWCC 2 cut(s) 1199, 1424
BmeRI GACNNNNNGTC 1 cut(s) 3061
BmeT110I CYCGRG 1 cut(s) 3194
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 4 cut(s) 1199, 1424, 2278, 2279
BmiI GGNNCC 4 cut(s) 1573, 2280, 2847, 3570
BmrI ACTGGG 1 cut(s) 2977
BmsI GCATC 6 cut(s) 719, 1527, 2327, 2451, 2610, 3206
BmuI ACTGGG 1 cut(s) 2977
BpiI GAAGAC 3 cut(s) 245, 278, 2098
BplI GAGNNNNNCTC 4 cut(s) 2321, 2353, 3254, 3286
BpmI CTGGAG 4 cut(s) 1590, 2721, 2939, 3162
Bpu10I CCTNAGC 3 cut(s) 1309, 2589, 3564
Bpu1102I GCTNAGC 1 cut(s) 1728
BpuEI CTTGAG 7 cut(s) 443, 701, 718, 917, 1373, 1438, 2941
BpuMI CCSGG 3 cut(s) 912, 1351, 3053
BsaAI YACGTR 1 cut(s) 3187
BsaBI GATNNNNATC 1 cut(s) 3147
BsaI GGTCTC 2 cut(s) 245, 2781
BsaJI CCNNGG 3 cut(s) 472, 1350, 2215
BsaWI WCCGGW 3 cut(s) 406, 599, 2291
BsaXI ACNNNNNCTCC 4 cut(s) 365, 395, 1893, 1923
Bsc4I CCNNNNNNNGG 6 cut(s) 406, 535, 2294, 2555, 2842, 2855
Bse118I RCCGGY 1 cut(s) 1632
Bse21I CCTNAGG 1 cut(s) 2425
Bse3DI GCAATG 3 cut(s) 1780, 2023, 3076
Bse8I GATNNNNATC 1 cut(s) 3147
BseAI TCCGGA 1 cut(s) 2291
BseBI CCWGG 9 cut(s) 474, 695, 1535, 1814, 1833, 2217, 2843, 3180, 3533
BseDI CCNNGG 3 cut(s) 472, 1350, 2215
BseGI GGATG 6 cut(s) 79, 542, 835, 2318, 3332, 3541
BseJI GATNNNNATC 1 cut(s) 3147
BseLI CCNNNNNNNGG 6 cut(s) 406, 535, 2294, 2555, 2842, 2855
BseMI GCAATG 3 cut(s) 1780, 2023, 3076
BseRI GAGGAG 1 cut(s) 2891
BseSI GKGCMC 2 cut(s) 2282, 3192
BseXI GCAGC 7 cut(s) 46, 798, 2574, 2577, 2984, 3017, 3349
BseYI CCCAGC 2 cut(s) 2314, 2854
BsgI GTGCAG 2 cut(s) 1582, 1917
BsiHKAI GWGCWC 1 cut(s) 3164
BsiHKCI CYCGRG 1 cut(s) 3194
BsiSI CCGG 8 cut(s) 407, 600, 911, 1350, 1633, 2292, 3052, 3463
BslFI GGGAC 6 cut(s) 1024, 2077, 2198, 2242, 2270, 3068
BslI CCNNNNNNNGG 6 cut(s) 406, 535, 2294, 2555, 2842, 2855
BsmAI GTCTC 7 cut(s) 245, 709, 863, 1100, 1930, 2781, 2875
BsmBI CGTCTC 1 cut(s) 709
BsmFI GGGAC 6 cut(s) 1024, 2077, 2198, 2242, 2270, 3068
BsmI GAATGC 2 cut(s) 2447, 3014
Bso31I GGTCTC 2 cut(s) 245, 2781
BsoBI CYCGRG 1 cut(s) 3194
Bsp120I GGGCCC 1 cut(s) 2278
Bsp1286I GDGCHC 4 cut(s) 2282, 2957, 3164, 3192
Bsp13I TCCGGA 1 cut(s) 2291
Bsp1407I TGTACA 1 cut(s) 2718
Bsp143I GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
Bsp1720I GCTNAGC 1 cut(s) 1728
BspACI CCGC 4 cut(s) 1414, 2073, 2509, 3466
BspEI TCCGGA 1 cut(s) 2291
BspHI TCATGA 1 cut(s) 2932
BspLI GGNNCC 4 cut(s) 1573, 2280, 2847, 3570
BspPI GGATC 2 cut(s) 735, 2227
BspQI GCTCTTC 1 cut(s) 1087
BspTI CTTAAG 1 cut(s) 1673
BspTNI GGTCTC 2 cut(s) 245, 2781
BsrDI GCAATG 3 cut(s) 1780, 2023, 3076
BsrFI RCCGGY 1 cut(s) 1632
BsrGI TGTACA 1 cut(s) 2718
BssAI RCCGGY 1 cut(s) 1632
BssECI CCNNGG 3 cut(s) 472, 1350, 2215
BssMI GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
Bst2UI CCWGG 9 cut(s) 474, 695, 1535, 1814, 1833, 2217, 2843, 3180, 3533
Bst6I CTCTTC 4 cut(s) 408, 1087, 1268, 2462
BstAFI CTTAAG 1 cut(s) 1673
BstAPI GCANNNNNTGC 1 cut(s) 758
BstAUI TGTACA 1 cut(s) 2718
BstBAI YACGTR 1 cut(s) 3187
BstC8I GCNNGC 6 cut(s) 763, 1451, 1680, 1806, 1819, 1888
BstEII GGTNACC 1 cut(s) 2352
BstENI CCTNNNNNAGG 1 cut(s) 2292
BstF5I GGATG 6 cut(s) 79, 542, 835, 2318, 3332, 3541
BstH2I RGCGCY 1 cut(s) 1257
BstHHI GCGC 1 cut(s) 1256
BstKTI GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
BstMAI GTCTC 7 cut(s) 245, 709, 863, 1100, 1930, 2781, 2875
BstMBI GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
BstNI CCWGG 9 cut(s) 474, 695, 1535, 1814, 1833, 2217, 2843, 3180, 3533
BstPI GGTNACC 1 cut(s) 2352
BstSFI CTRYAG 2 cut(s) 3238, 3285
BstSLI GKGCMC 2 cut(s) 2282, 3192
BstV1I GCAGC 7 cut(s) 46, 798, 2574, 2577, 2984, 3017, 3349
BstV2I GAAGAC 3 cut(s) 245, 278, 2098
BstX2I RGATCY 3 cut(s) 727, 1246, 2219
BstYI RGATCY 3 cut(s) 727, 1246, 2219
Bsu36I CCTNAGG 1 cut(s) 2425
BsuI GTATCC 1 cut(s) 2474
BtgZI GCGATG 1 cut(s) 2428
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 6 cut(s) 79, 542, 835, 2318, 3332, 3541
BtsI GCAGTG 1 cut(s) 785
Cac8I GCNNGC 6 cut(s) 763, 1451, 1680, 1806, 1819, 1888
CaiI CAGNNNCTG 4 cut(s) 565, 1844, 2568, 3424
CciI TCATGA 1 cut(s) 2932
CfoI GCGC 1 cut(s) 1256
Cfr10I RCCGGY 1 cut(s) 1632
Cfr13I GGNCC 4 cut(s) 1199, 1424, 2278, 2279
CseI GACGC 1 cut(s) 691
CsiI ACCWGGT 1 cut(s) 2841
DpnI GATC 7 cut(s) 330, 729, 1248, 1401, 2030, 2184, 2221
DpnII GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
DraI TTTAAA 1 cut(s) 3499
DraIII CACNNNGTG 1 cut(s) 2369
DrdI GACNNNNNNGTC 1 cut(s) 3527
DriI GACNNNNNGTC 1 cut(s) 3061
DseDI GACNNNNNNGTC 1 cut(s) 3527
EaeI YGGCCR 2 cut(s) 3467, 3489
Eam1104I CTCTTC 4 cut(s) 408, 1087, 1268, 2462
Eam1105I GACNNNNNGTC 1 cut(s) 3061
EarI CTCTTC 4 cut(s) 408, 1087, 1268, 2462
Eco147I AGGCCT 2 cut(s) 189, 3283
Eco24I GRGCYC 2 cut(s) 2282, 2957
Eco31I GGTCTC 2 cut(s) 245, 2781
Eco32I GATATC 1 cut(s) 1169
Eco47I GGWCC 2 cut(s) 1199, 1424
Eco47III AGCGCT 1 cut(s) 1255
Eco81I CCTNAGG 1 cut(s) 2425
Eco88I CYCGRG 1 cut(s) 3194
Eco91I GGTNACC 1 cut(s) 2352
EcoNI CCTNNNNNAGG 1 cut(s) 2292
EcoO109I RGGNCCY 1 cut(s) 2279
EcoO65I GGTNACC 1 cut(s) 2352
EcoRII CCWGG 9 cut(s) 472, 693, 1533, 1812, 1831, 2215, 2841, 3178, 3531
EcoRV GATATC 1 cut(s) 1169
EcoT38I GRGCYC 2 cut(s) 2282, 2957
Esp3I CGTCTC 1 cut(s) 709
FalI AAGNNNNNCTT 4 cut(s) 2627, 2659, 3419, 3451
FaqI GGGAC 6 cut(s) 1024, 2077, 2198, 2242, 2270, 3068
FauI CCCGC 1 cut(s) 2080
FblI GTMKAC 1 cut(s) 3237
Fnu4HI GCNGC 8 cut(s) 60, 787, 2563, 2566, 2973, 3031, 3338, 3467
FokI GGATG 6 cut(s) 86, 529, 842, 2305, 3339, 3548
FriOI GRGCYC 2 cut(s) 2282, 2957
Fsp4HI GCNGC 8 cut(s) 60, 787, 2563, 2566, 2973, 3031, 3338, 3467
GlaI GCGC 1 cut(s) 1255
GluI GCNGC 8 cut(s) 60, 787, 2563, 2566, 2973, 3031, 3338, 3467
GsaI CCCAGC 2 cut(s) 2318, 2858
GsuI CTGGAG 4 cut(s) 1590, 2721, 2939, 3162
HaeII RGCGCY 1 cut(s) 1257
HapII CCGG 8 cut(s) 407, 600, 911, 1350, 1633, 2292, 3052, 3463
HgaI GACGC 1 cut(s) 691
HhaI GCGC 1 cut(s) 1256
Hin6I GCGC 1 cut(s) 1254
HinP1I GCGC 1 cut(s) 1254
HincII GTYRAC 3 cut(s) 373, 451, 2130
HindII GTYRAC 3 cut(s) 373, 451, 2130
HindIII AAGCTT 6 cut(s) 86, 861, 1151, 1676, 2149, 2762
HpaI GTTAAC 1 cut(s) 2130
HpaII CCGG 8 cut(s) 407, 600, 911, 1350, 1633, 2292, 3052, 3463
Hpy166II GTNNAC 8 cut(s) 373, 451, 1949, 2002, 2087, 2130, 2719, 3238
Hpy8I GTNNAC 8 cut(s) 373, 451, 1949, 2002, 2087, 2130, 2719, 3238
HpyCH4IV ACGT 3 cut(s) 210, 2607, 3186
HpySE526I ACGT 3 cut(s) 210, 2607, 3186
HspAI GCGC 1 cut(s) 1254
Kpn2I TCCGGA 1 cut(s) 2291
KspAI GTTAAC 1 cut(s) 2130
Kzo9I GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
LguI GCTCTTC 1 cut(s) 1087
LmnI GCTCC 5 cut(s) 1021, 1309, 1446, 1571, 1643
Lsp1109I GCAGC 7 cut(s) 46, 798, 2574, 2577, 2984, 3017, 3349
LweI GCATC 6 cut(s) 719, 1527, 2327, 2451, 2610, 3206
MabI ACCWGGT 1 cut(s) 2841
MaeII ACGT 3 cut(s) 210, 2607, 3186
MaeIII GTNAC 7 cut(s) 587, 1617, 2246, 2352, 2369, 2773, 3182
MalI GATC 7 cut(s) 330, 729, 1248, 1401, 2030, 2184, 2221
MboI GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
MfeI CAATTG 3 cut(s) 1395, 2169, 2729
MflI RGATCY 3 cut(s) 727, 1246, 2219
MhlI GDGCHC 4 cut(s) 2282, 2957, 3164, 3192
MlsI TGGCCA 1 cut(s) 3491
MluNI TGGCCA 1 cut(s) 3491
MlyI GAGTC 4 cut(s) 1932, 1932, 2184, 2224
MmeI TCCRAC 8 cut(s) 287, 366, 408, 1488, 1951, 2573, 2851, 3304
Mox20I TGGCCA 1 cut(s) 3491
MroI TCCGGA 1 cut(s) 2291
MroXI GAANNNNTTC 2 cut(s) 1840, 2544
MscI TGGCCA 1 cut(s) 3491
MseI TTAA 5 cut(s) 1674, 2129, 2162, 2943, 3498
MslI CAYNNNNRTG 3 cut(s) 1604, 1991, 3153
Msp20I TGGCCA 1 cut(s) 3491
MspA1I CMGCKG 5 cut(s) 59, 1727, 2302, 2509, 2568
MspCI CTTAAG 1 cut(s) 1673
MspI CCGG 8 cut(s) 407, 600, 911, 1350, 1633, 2292, 3052, 3463
MunI CAATTG 3 cut(s) 1395, 2169, 2729
Mva1269I GAATGC 2 cut(s) 2447, 3014
MvaI CCWGG 9 cut(s) 474, 695, 1535, 1814, 1833, 2217, 2843, 3180, 3533
NciI CCSGG 3 cut(s) 912, 1351, 3053
NdeII GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
NlaIV GGNNCC 4 cut(s) 1573, 2280, 2847, 3570
NmeAIII GCCGAG 2 cut(s) 686, 1210
NmuCI GTSAC 4 cut(s) 1617, 2246, 2369, 2773
PagI TCATGA 1 cut(s) 2932
PceI AGGCCT 2 cut(s) 189, 3283
PciSI GCTCTTC 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 1 cut(s) 3192
PctI GAATGC 2 cut(s) 2447, 3014
PdmI GAANNNNTTC 2 cut(s) 1840, 2544
PfoI TCCNGGA 3 cut(s) 693, 1831, 3531
PkrI GCNGC 8 cut(s) 61, 788, 2564, 2567, 2974, 3032, 3339, 3468
PleI GAGTC 4 cut(s) 1931, 1932, 2183, 2224
PpsI GAGTC 4 cut(s) 1931, 1932, 2183, 2224
Ppu21I YACGTR 1 cut(s) 3187
Psp6I CCWGG 9 cut(s) 472, 693, 1533, 1812, 1831, 2215, 2841, 3178, 3531
PspEI GGTNACC 1 cut(s) 2352
PspFI CCCAGC 2 cut(s) 2314, 2854
PspGI CCWGG 9 cut(s) 472, 693, 1533, 1812, 1831, 2215, 2841, 3178, 3531
PspN4I GGNNCC 4 cut(s) 1573, 2280, 2847, 3570
PspOMI GGGCCC 1 cut(s) 2278
PspPI GGNCC 4 cut(s) 1199, 1424, 2278, 2279
PstNI CAGNNNCTG 4 cut(s) 565, 1844, 2568, 3424
PsuI RGATCY 3 cut(s) 727, 1246, 2219
PvuII CAGCTG 4 cut(s) 59, 1727, 2302, 2568
RseI CAYNNNNRTG 3 cut(s) 1604, 1991, 3153
SapI GCTCTTC 1 cut(s) 1087
SaqAI TTAA 5 cut(s) 1674, 2129, 2162, 2943, 3498
SatI GCNGC 8 cut(s) 60, 787, 2563, 2566, 2973, 3031, 3338, 3467
Sau3AI GATC 7 cut(s) 328, 727, 1246, 1399, 2028, 2182, 2219
Sau96I GGNCC 4 cut(s) 1199, 1424, 2278, 2279
ScaI AGTACT 2 cut(s) 1138, 3200
SchI GAGTC 4 cut(s) 1932, 1932, 2184, 2224
SduI GDGCHC 4 cut(s) 2282, 2957, 3164, 3192
SexAI ACCWGGT 1 cut(s) 2841
SfaNI GCATC 6 cut(s) 719, 1527, 2327, 2451, 2610, 3206
SfcI CTRYAG 2 cut(s) 3238, 3285
SinI GGWCC 2 cut(s) 1199, 1424
SmiMI CAYNNNNRTG 3 cut(s) 1604, 1991, 3153
SmlI CTYRAG 8 cut(s) 422, 716, 733, 932, 1388, 1417, 1673, 2956
SmoI CTYRAG 8 cut(s) 422, 716, 733, 932, 1388, 1417, 1673, 2956
SpeI ACTAGT 1 cut(s) 884
SseBI AGGCCT 2 cut(s) 189, 3283
SsiI CCGC 4 cut(s) 1414, 2073, 2509, 3466
SspI AATATT 1 cut(s) 2140
StuI AGGCCT 2 cut(s) 189, 3283
TaiI ACGT 3 cut(s) 213, 2610, 3189
TaqI TCGA 8 cut(s) 222, 1586, 1941, 2181, 2491, 3141, 3214, 3372
TaqII GACCGA 2 cut(s) 402, 2805
TatI WGTACW 5 cut(s) 297, 1136, 2718, 2898, 3198
TauI GCSGC 1 cut(s) 3469
Tru1I TTAA 5 cut(s) 1674, 2129, 2162, 2943, 3498
Tru9I TTAA 5 cut(s) 1674, 2129, 2162, 2943, 3498
TseFI GTSAC 4 cut(s) 1617, 2246, 2369, 2773
TseI GCWGC 7 cut(s) 59, 786, 2562, 2565, 2972, 3030, 3337
Tsp45I GTSAC 4 cut(s) 1617, 2246, 2369, 2773
TspDTI ATGAA 8 cut(s) 168, 303, 531, 1949, 2918, 2921, 3074, 3099
Vha464I CTTAAG 1 cut(s) 1673
VpaK11BI GGWCC 2 cut(s) 1199, 1424
XagI CCTNNNNNAGG 1 cut(s) 2292
XapI RAATTY 4 cut(s) 46, 981, 1126, 1980
XbaI TCTAGA 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 3237
XmnI GAANNNNTTC 2 cut(s) 1840, 2544
ZrmI AGTACT 2 cut(s) 1138, 3200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.