Rh5BG509300
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002466
Physical Location & Seq
Forward (+)
80996398 .. 80999976
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG509300.1

Sequence Viewer

Length: 3579 bp
ATGATGGCTTTGTTCTGTTTTCTGCTTCTGCTTCTTTTTCACTCTCTAAATTTCTCTTCAGCTGCTTCTAATGAGGATGTGGTGAAGAAGCTTTTGTTGCAGTTCAAGCAATCCTCTGTTCAATCTGACCCTCAAGGTTTCTTATCTGACTGGAAATCTGATTCTTCAACTGCTCCTTGTTCATGGAAAGGCCTGACTTGCTCTGAAGGCCACGTCATCACCCTCGACCTCTCCTCTCATGGTCTCATTGGAAGTCTTTACCTTCCAACTCTCACAGCCTTGCCAAGTCTTCAAAATCTGTACTTGCAAAACAATTCATTCTCTGCTACTGATCTTTCAGTTTCCAACATTACCTCATGTAGTCTTGTTACAGTTGACTTGTCCTCCAACAACATCACAAGCCCTCTTCCGGTTCGGTCTTTTCTTGAGGGTTGTGAGAATCTGGCTTTGGTCAACCTCTCTGGCAATTCAATCCCAGGAGGGAGTTTCAGCTTTGGTGCTTCCTTGCTTCAGCTTGACATCTCTAGAAACCTGATTTCGGATTCATCCTTGTTGACTTGCCAGAATCTGAATCTGCTCAATGTTTCCGGTAACAAGCTGACCGGAAAACTGAGTGATTCTATCTTGCCTTGCAAGAATCTGACGACACTTGACCTTTCATACAATGCTTTGTCTGGGGAGATACCAAACACTTTCCTGGAAAGCGTCTCGGCATCTCTCAAGTATTTGGATCTCTCAAGCAACAATTTCTCTGCAAAGTTTGCAAGCCTTGATTTCGGGCAGTGTAGCAGCCTCACTCTGCTGAAGCTATCACACAATAATCTCTATGGGGATGATTTTCCTTCAAGCCTTGCTAACTGTCAAGCTTTGGAGACACTGAACCTGACTAGTAATAAGTTGCAGGACAAGATTCCGGGTGCTTTGTTGGGAAATCTCAAGAAGTTGAGGCAACTCTTTCTGGGTCGTAATCAGTTTTCAGGTGAAATTCCAGCTGAATTAGGAAAGGCTTGTGGGACACTCAAGGAGCTTGATATTTCCGATAACATTCTTACTGGTGAATTGCCTTCAAATTTTGTGTCGTGCTCTTTTTTGGTTAGTCTCAATCTTGGTCGCAATCAGCTGTCAGGAAATTTCCTCAGTACTGTTGTGAGTAAGCTTCCGAGTTTGAGATATCTCTATGTACCCTTCAACAACATCACTGGTCCTGTGCCACCATCTATTACCAGTGGTACTCGGCTTCAAGTGCTCGATCTCAGCGCTAATAACTTCACAGGGAATGTTCCTTCAGTGTTTTGCTTCTCCAATGCTCCCTCAGCTTTGGAAAAGATACTTCTAGCCAACAATTTTCTCTCCGGGACTGTGCCATCGGAACTTGGAAACTGCAAGAACCTCAAGGCAATTGATCTTAGTTTCAACAGCTTGAGTGGTCCAATTCCTTCAGAAGTTTGGAGCTTGCCAAATCTTTCCGACTTGGTTATGTGGGCAAACAATCTCACTGGTGAAATCCCAGAAGGCATTTGCATCAATGGAGGAAACCTGGAAACCCTGATTCTCAACAATAATCTCATTACTGGAGCCATTCCACAGTCAATTGCCAACTGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGGCTTACAGGAGCAATCCCTTCAGGTATTGGAAATCTCCTTAAGCTTGCTATACTTCAGTTGGGTAATAATTCACTGTCTGGTCAGATTCCAGCTGAGCTTGGCAAGTGTCAAAGCCTCATTTGGCTTGACCTGAACAGCAATGACCTAAATGGTTCTATCCCATCAGAGCTTGCTAACCAGGCCGGCCTAGTCATTCCTGGAACAGTTTCTGGGAAGCAGTTTGCATTTGTTAGAAATGAGGGTGGTACAGCTTGCAGGGGTGCGGGAGGACTAGTTGAGTTTGAGGGAGTACGACCTCAGAGACTAGAAAGTTTACCTATGGTTCATTCTTGTCCATCAACTAGAATTTACACAGGAATGGCAGTTTACACATTCACCAGCAATGGAAGCATGATCTTCCTTGACGTTTCCTACAATTACTTGTCTGGGACTATTCCCGCAAACTTAGGTACACTGTCCTATTTGCAAGTCTTCAATCTAGGACACAATATGTTAAGTGGAAATATTCCAGAAAGCTTTGGAGGATTAAAAGCAATTGGCGTCCTGGATCTCTCTCACAATAATCTTCAAGGTTATGTCCCAGGATCTTTGGGGAATCTCTCTTTCCTGAGTGACCTTGATGTGTCTAACAACAACCTCACGGGCCCTGTCCCTTCCGGAGGTCAGCTGACAACTTTCCCAGCATCCAGATATGAGAACAACTCTGGCCTTTGTGGGTTACCCCTGCCACCGTGTGGCTCTCAAAGACATTCAGCAGAGAGAGTTAGGGGAAAGAAGCCATCTATGGCTTCAGGAATGGTTATTGGCATCGCATTCTTTCTCTTCTGTATCCTTATACTTGCATTAGCTCTATATCGAGTGAAGAAGTACCAGCGGAAAGAGGCGAAGAGAGAAAAATATATTGAAAGCCTTCCGACTTCAGGTAGCAGCAGCTGGAAACTTTCCAGTGTTCCTGAGCCTCTCAGCATCAACGTAGCCACATTTGAAAAACCTTTGAGGAAGCTGACCTTCGCCCATTTACTTGAAGCCACCAATGGTTTCAGTGCTGATAGCTTGATTGGATCTGGAGGGTTTGGTGAGGTCTACAAGGCACAATTGGGAGATGGCTGTGTTGTTGCAATCAAGAAGCTTATTCAAGTCACAGGTCAAGGAGACCGAGAGTTTATGGCAGAAATGGAAACTATTGGGAAAATCAAACACCGAAACCTGGTTCCTTTGCTGGGGTACTGCAAGGTTGGAGAGGAGAGACTTCTTGTGTATGAGTACATGAAATGGGGAAGTCTGGAGAGTGTTTTTCATGATAAGATTAAGGGAGGGGGCACCAGGCTTGACTGGGCAGCACGAAAGAAGATTGCCATAGGGTCTGCTAGAGGTCTAGCATTCCTTCACCATAGCTGCATACCTCATATTATCCACCGGGACATGAAGTCTAGCAATGTTCTTATAGATGAAAACTTTGAGGCCAGAGTATCAGATTTTGGCATGGCAAGATTGGTCAATGCCCTCGATACTCATCTCAGTGTGAGCACCCTAGCAGGAACTCCAGGTTACGTGCCCCCCGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGAGATGTCTACAGTTATGGTGTTATACTGCTAGAGCTTCTCTCAGGAAAAAGGCCTATAGACCCGTCAACATTTGGTGATGACAACAACCTTGTTGGATGGGCAAAGCAGCTTCAAAGAGAAAAGAGATGGAATGAGATACTCGACACTGAGTTGATGACACAAAAATCTGGTGAGGCTGAACTATACCAGTATCTGAAGATTGCCTTTGAGTGCCTAGACGACAGGCCATTCCGGCGGCCAACAATGATTCAGGTTATGGCTATGTTTAAAGAGCTTCAGGTTGATTCGGACAGTGATGTCCTGGATGGTTTTTCACTCAAAGACACTGTTGCTGAGGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1192

Amino Acids

128.87

Weight (kDa)

6.05

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 68 2.9e-09 Leucine rich repeat N-terminal domain
LRR_4 PF12799 212 - 257 9.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 339 - 399 5.2e-06 Leucine rich repeat
Island PF20141 608 - 672 2.4e-30 Brassinosteroid receptor island domain
LRR_8 PF13855 680 - 735 1.8e-07 Leucine rich repeat
LRR_8 PF13855 701 - 759 1.7e-07 Leucine rich repeat
Pkinase PF00069 895 - 1165 6.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 896 - 1165 1.4e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3530
AccB1I GGYRCC 1 cut(s) 2957
AccB7I CCANNNNNTGG 1 cut(s) 2372
AccI GTMKAC 2 cut(s) 2721, 3240
AccIII TCCGGA 1 cut(s) 2294
AciI CCGC 4 cut(s) 1901, 2076, 2512, 3469
AclWI GGATC 4 cut(s) 738, 2193, 2230, 2707
AcoI YGGCCR 1 cut(s) 3470
AcsI RAATTY 5 cut(s) 49, 984, 1069, 1129, 1983
AcyI GRCGYC 1 cut(s) 2178
AdeI CACNNNGTG 1 cut(s) 2372
AfeI AGCGCT 1 cut(s) 1258
AfiI CCNNNNNNNGG 7 cut(s) 409, 538, 2297, 2372, 2558, 2845, 2858
AflII CTTAAG 1 cut(s) 1676
AhdI GACNNNNNGTC 1 cut(s) 3064
AhlI ACTAGT 2 cut(s) 887, 1909
AjiI CACGTC 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
Alw21I GWGCWC 3 cut(s) 1085, 1248, 3167
Alw26I GTCTC 7 cut(s) 248, 712, 866, 1103, 1933, 2784, 2878
AlwI GGATC 4 cut(s) 738, 2193, 2230, 2707
AlwNI CAGNNNCTG 4 cut(s) 568, 1847, 2571, 3427
Ama87I CYCGRG 1 cut(s) 3197
Aor13HI TCCGGA 1 cut(s) 2294
Aor51HI AGCGCT 1 cut(s) 1258
ApaI GGGCCC 1 cut(s) 2285
ApeKI GCWGC 7 cut(s) 62, 789, 2565, 2568, 2975, 3033, 3340
ApoI RAATTY 5 cut(s) 49, 984, 1069, 1129, 1983
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
Asp700I GAANNNNTTC 2 cut(s) 1843, 2547
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 4 cut(s) 1202, 1427, 2281, 2282
AsuC2I CCSGG 3 cut(s) 915, 1354, 3056
AvaI CYCGRG 1 cut(s) 3197
AvaII GGWCC 2 cut(s) 1202, 1427
BaeGI GKGCMC 3 cut(s) 2285, 2960, 3195
BanI GGYRCC 1 cut(s) 2957
BanII GRGCYC 1 cut(s) 2285
BbsI GAAGAC 2 cut(s) 281, 2101
Bbv12I GWGCWC 3 cut(s) 1085, 1248, 3167
BbvCI CCTCAGC 2 cut(s) 1312, 3567
BbvI GCAGC 7 cut(s) 49, 801, 2577, 2580, 2987, 3020, 3352
BccI CCATC 9 cut(s) 1222, 1372, 1807, 1981, 2425, 2735, 3324, 3354, 3533
BciVI GTATCC 1 cut(s) 2477
BcnI CCSGG 3 cut(s) 915, 1354, 3056
BcoDI GTCTC 7 cut(s) 248, 712, 866, 1103, 1933, 2784, 2878
BcuI ACTAGT 2 cut(s) 887, 1909
BfmI CTRYAG 2 cut(s) 3241, 3288
BfoI RGCGCY 1 cut(s) 1260
BfrI CTTAAG 1 cut(s) 1676
BfuI GTATCC 1 cut(s) 2477
BglI GCCNNNNNGGC 1 cut(s) 3466
BisI GCNGC 8 cut(s) 63, 790, 2566, 2569, 2976, 3034, 3341, 3470
BlpI GCTNAGC 1 cut(s) 1731
BlsI GCNGC 8 cut(s) 64, 791, 2567, 2570, 2977, 3035, 3342, 3471
BmcAI AGTACT 2 cut(s) 1141, 3203
Bme18I GGWCC 2 cut(s) 1202, 1427
BmeRI GACNNNNNGTC 1 cut(s) 3064
BmeT110I CYCGRG 1 cut(s) 3197
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 4 cut(s) 1202, 1427, 2281, 2282
BmiI GGNNCC 5 cut(s) 1576, 2283, 2850, 2959, 3573
BmrI ACTGGG 1 cut(s) 2980
BmsI GCATC 6 cut(s) 722, 1530, 2330, 2454, 2613, 3209
BmuI ACTGGG 1 cut(s) 2980
BpiI GAAGAC 2 cut(s) 281, 2101
BplI GAGNNNNNCTC 4 cut(s) 2324, 2356, 3257, 3289
BpmI CTGGAG 4 cut(s) 1593, 2724, 2942, 3165
Bpu10I CCTNAGC 3 cut(s) 1312, 2592, 3567
Bpu1102I GCTNAGC 1 cut(s) 1731
BpuEI CTTGAG 8 cut(s) 117, 446, 704, 721, 920, 1004, 1376, 1441
BpuMI CCSGG 3 cut(s) 915, 1354, 3056
BsaAI YACGTR 1 cut(s) 3190
BsaBI GATNNNNATC 1 cut(s) 3150
BsaHI GRCGYC 1 cut(s) 2178
BsaI GGTCTC 2 cut(s) 248, 2784
BsaJI CCNNGG 2 cut(s) 475, 2218
BsaWI WCCGGW 4 cut(s) 409, 587, 602, 2294
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 1896, 1926
Bsc4I CCNNNNNNNGG 7 cut(s) 409, 538, 2297, 2372, 2558, 2845, 2858
Bse118I RCCGGY 2 cut(s) 1635, 1820
Bse1I ACTGG 9 cut(s) 155, 1057, 1204, 1224, 1501, 1576, 2583, 2975, 3421
Bse3DI GCAATG 3 cut(s) 1783, 2026, 3079
Bse8I GATNNNNATC 1 cut(s) 3150
BseAI TCCGGA 1 cut(s) 2294
BseDI CCNNGG 2 cut(s) 475, 2218
BseGI GGATG 6 cut(s) 82, 545, 838, 2321, 3335, 3544
BseJI GATNNNNATC 1 cut(s) 3150
BseLI CCNNNNNNNGG 7 cut(s) 409, 538, 2297, 2372, 2558, 2845, 2858
BseMI GCAATG 3 cut(s) 1783, 2026, 3079
BseNI ACTGG 9 cut(s) 155, 1057, 1204, 1224, 1501, 1576, 2583, 2975, 3421
BseRI GAGGAG 2 cut(s) 223, 2894
BseSI GKGCMC 3 cut(s) 2285, 2960, 3195
BseXI GCAGC 7 cut(s) 49, 801, 2577, 2580, 2987, 3020, 3352
BseYI CCCAGC 2 cut(s) 2317, 2857
BsgI GTGCAG 1 cut(s) 1585
BshNI GGYRCC 1 cut(s) 2957
BsiHKAI GWGCWC 3 cut(s) 1085, 1248, 3167
BsiHKCI CYCGRG 1 cut(s) 3197
BslFI GGGAC 6 cut(s) 1027, 1369, 2080, 2201, 2273, 3071
BslI CCNNNNNNNGG 7 cut(s) 409, 538, 2297, 2372, 2558, 2845, 2858
BsmAI GTCTC 7 cut(s) 248, 712, 866, 1103, 1933, 2784, 2878
BsmBI CGTCTC 1 cut(s) 712
BsmFI GGGAC 6 cut(s) 1027, 1369, 2080, 2201, 2273, 3071
BsmI GAATGC 2 cut(s) 2450, 3017
Bso31I GGTCTC 2 cut(s) 248, 2784
BsoBI CYCGRG 1 cut(s) 3197
Bsp120I GGGCCC 1 cut(s) 2281
Bsp1286I GDGCHC 6 cut(s) 1085, 1248, 2285, 2960, 3167, 3195
Bsp13I TCCGGA 1 cut(s) 2294
Bsp143I GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
Bsp1720I GCTNAGC 1 cut(s) 1731
BspACI CCGC 4 cut(s) 1901, 2076, 2512, 3469
BspEI TCCGGA 1 cut(s) 2294
BspHI TCATGA 1 cut(s) 2935
BspLI GGNNCC 5 cut(s) 1576, 2283, 2850, 2959, 3573
BspPI GGATC 4 cut(s) 738, 2193, 2230, 2707
BspT107I GGYRCC 1 cut(s) 2957
BspTI CTTAAG 1 cut(s) 1676
BspTNI GGTCTC 2 cut(s) 248, 2784
BsrDI GCAATG 3 cut(s) 1783, 2026, 3079
BsrFI RCCGGY 2 cut(s) 1635, 1820
BsrI ACTGG 9 cut(s) 155, 1057, 1204, 1224, 1501, 1576, 2583, 2975, 3421
BssAI RCCGGY 2 cut(s) 1635, 1820
BssECI CCNNGG 2 cut(s) 475, 2218
BssMI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
BssNI GRCGYC 1 cut(s) 2178
Bst6I CTCTTC 4 cut(s) 61, 411, 2465, 2519
BstACI GRCGYC 1 cut(s) 2178
BstAFI CTTAAG 1 cut(s) 1676
BstAPI GCANNNNNTGC 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 3190
BstC8I GCNNGC 6 cut(s) 766, 1454, 1683, 1809, 1822, 1891
BstEII GGTNACC 1 cut(s) 2355
BstENI CCTNNNNNAGG 1 cut(s) 2295
BstF5I GGATG 6 cut(s) 82, 545, 838, 2321, 3335, 3544
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 8 cut(s) 334, 733, 1252, 1405, 2034, 2188, 2225, 2702
BstMAI GTCTC 7 cut(s) 248, 712, 866, 1103, 1933, 2784, 2878
BstMBI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
BstPI GGTNACC 1 cut(s) 2355
BstSFI CTRYAG 2 cut(s) 3241, 3288
BstSLI GKGCMC 3 cut(s) 2285, 2960, 3195
BstV1I GCAGC 7 cut(s) 49, 801, 2577, 2580, 2987, 3020, 3352
BstV2I GAAGAC 2 cut(s) 281, 2101
BstX2I RGATCY 4 cut(s) 730, 2185, 2222, 2699
BstYI RGATCY 4 cut(s) 730, 2185, 2222, 2699
BsuI GTATCC 1 cut(s) 2477
BtgZI GCGATG 1 cut(s) 2431
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 6 cut(s) 82, 545, 838, 2321, 3335, 3544
BtsI GCAGTG 1 cut(s) 788
Cac8I GCNNGC 6 cut(s) 766, 1454, 1683, 1809, 1822, 1891
CaiI CAGNNNCTG 4 cut(s) 568, 1847, 2571, 3427
CciI TCATGA 1 cut(s) 2935
CfoI GCGC 1 cut(s) 1259
Cfr10I RCCGGY 2 cut(s) 1635, 1820
Cfr13I GGNCC 4 cut(s) 1202, 1427, 2281, 2282
CseI GACGC 2 cut(s) 694, 2167
CsiI ACCWGGT 1 cut(s) 2844
CviAII CATG 9 cut(s) 183, 239, 357, 2029, 2905, 2936, 3061, 3121, 3576
DpnI GATC 8 cut(s) 333, 732, 1251, 1404, 2033, 2187, 2224, 2701
DpnII GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
DraI TTTAAA 1 cut(s) 3502
DraIII CACNNNGTG 1 cut(s) 2372
DrdI GACNNNNNNGTC 1 cut(s) 3530
DriI GACNNNNNGTC 1 cut(s) 3064
DseDI GACNNNNNNGTC 1 cut(s) 3530
EaeI YGGCCR 1 cut(s) 3470
Eam1104I CTCTTC 4 cut(s) 61, 411, 2465, 2519
Eam1105I GACNNNNNGTC 1 cut(s) 3064
EarI CTCTTC 4 cut(s) 61, 411, 2465, 2519
Eco147I AGGCCT 2 cut(s) 192, 3286
Eco24I GRGCYC 1 cut(s) 2285
Eco31I GGTCTC 2 cut(s) 248, 2784
Eco32I GATATC 1 cut(s) 1172
Eco47I GGWCC 2 cut(s) 1202, 1427
Eco47III AGCGCT 1 cut(s) 1258
Eco88I CYCGRG 1 cut(s) 3197
Eco91I GGTNACC 1 cut(s) 2355
EcoNI CCTNNNNNAGG 1 cut(s) 2295
EcoO109I RGGNCCY 1 cut(s) 2282
EcoO65I GGTNACC 1 cut(s) 2355
EcoRV GATATC 1 cut(s) 1172
EcoT38I GRGCYC 1 cut(s) 2285
Esp3I CGTCTC 1 cut(s) 712
FaeI CATG 9 cut(s) 186, 242, 360, 2032, 2908, 2939, 3064, 3124, 3579
FalI AAGNNNNNCTT 4 cut(s) 2630, 2662, 3422, 3454
FaqI GGGAC 6 cut(s) 1027, 1369, 2080, 2201, 2273, 3071
FatI CATG 9 cut(s) 182, 238, 356, 2028, 2904, 2935, 3060, 3120, 3575
FauI CCCGC 2 cut(s) 1894, 2083
FblI GTMKAC 2 cut(s) 2721, 3240
Fnu4HI GCNGC 8 cut(s) 63, 790, 2566, 2569, 2976, 3034, 3341, 3470
FokI GGATG 6 cut(s) 89, 532, 845, 2308, 3342, 3551
FriOI GRGCYC 1 cut(s) 2285
FseI GGCCGGCC 1 cut(s) 1824
Fsp4HI GCNGC 8 cut(s) 63, 790, 2566, 2569, 2976, 3034, 3341, 3470
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 8 cut(s) 63, 790, 2566, 2569, 2976, 3034, 3341, 3470
GsaI CCCAGC 2 cut(s) 2321, 2861
GsuI CTGGAG 4 cut(s) 1593, 2724, 2942, 3165
HaeII RGCGCY 1 cut(s) 1260
HgaI GACGC 2 cut(s) 694, 2167
HhaI GCGC 1 cut(s) 1259
Hin1I GRCGYC 1 cut(s) 2178
Hin1II CATG 9 cut(s) 186, 242, 360, 2032, 2908, 2939, 3064, 3124, 3579
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HincII GTYRAC 4 cut(s) 376, 454, 555, 3300
HindII GTYRAC 4 cut(s) 376, 454, 555, 3300
HindIII AAGCTT 6 cut(s) 89, 864, 1154, 1679, 2152, 2765
Hpy166II GTNNAC 9 cut(s) 376, 454, 555, 1952, 2005, 2090, 2722, 3241, 3300
Hpy8I GTNNAC 9 cut(s) 376, 454, 555, 1952, 2005, 2090, 2722, 3241, 3300
HpyCH4IV ACGT 4 cut(s) 213, 2043, 2610, 3189
HpySE526I ACGT 4 cut(s) 213, 2043, 2610, 3189
Hsp92I GRCGYC 1 cut(s) 2178
Hsp92II CATG 9 cut(s) 186, 242, 360, 2032, 2908, 2939, 3064, 3124, 3579
HspAI GCGC 1 cut(s) 1257
Kpn2I TCCGGA 1 cut(s) 2294
KroI GCCGGC 1 cut(s) 1820
KroNI GCCGGC 1 cut(s) 1822
Kzo9I GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
LmnI GCTCC 6 cut(s) 178, 1024, 1312, 1449, 1574, 1646
Lsp1109I GCAGC 7 cut(s) 49, 801, 2577, 2580, 2987, 3020, 3352
LweI GCATC 6 cut(s) 722, 1530, 2330, 2454, 2613, 3209
MabI ACCWGGT 1 cut(s) 2844
MaeII ACGT 4 cut(s) 213, 2043, 2610, 3189
MaeIII GTNAC 7 cut(s) 367, 590, 1620, 2249, 2355, 2776, 3185
MalI GATC 8 cut(s) 333, 732, 1251, 1404, 2033, 2187, 2224, 2701
MboI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
MfeI CAATTG 4 cut(s) 1398, 1590, 2172, 2732
MflI RGATCY 4 cut(s) 730, 2185, 2222, 2699
MhlI GDGCHC 6 cut(s) 1085, 1248, 2285, 2960, 3167, 3195
MmeI TCCRAC 7 cut(s) 290, 369, 411, 1491, 2576, 2854, 3307
MroI TCCGGA 1 cut(s) 2294
MroNI GCCGGC 1 cut(s) 1820
MroXI GAANNNNTTC 2 cut(s) 1843, 2547
MseI TTAA 5 cut(s) 1677, 2132, 2165, 2946, 3501
MslI CAYNNNNRTG 3 cut(s) 1607, 1994, 3156
MspA1I CMGCKG 7 cut(s) 62, 992, 1120, 1730, 2305, 2512, 2571
MspCI CTTAAG 1 cut(s) 1676
MunI CAATTG 4 cut(s) 1398, 1590, 2172, 2732
Mva1269I GAATGC 2 cut(s) 2450, 3017
NaeI GCCGGC 1 cut(s) 1822
NciI CCSGG 3 cut(s) 915, 1354, 3056
NdeII GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
NgoMIV GCCGGC 1 cut(s) 1820
NlaIII CATG 9 cut(s) 186, 242, 360, 2032, 2908, 2939, 3064, 3124, 3579
NlaIV GGNNCC 5 cut(s) 1576, 2283, 2850, 2959, 3573
NmeAIII GCCGAG 2 cut(s) 689, 1213
NmuCI GTSAC 3 cut(s) 1620, 2249, 2776
PagI TCATGA 1 cut(s) 2935
PceI AGGCCT 2 cut(s) 192, 3286
PcsI WCGNNNNNNNCGW 1 cut(s) 3195
PctI GAATGC 2 cut(s) 2450, 3017
PdiI GCCGGC 1 cut(s) 1822
PdmI GAANNNNTTC 2 cut(s) 1843, 2547
PflMI CCANNNNNTGG 1 cut(s) 2372
PfoI TCCNGGA 5 cut(s) 696, 1352, 1834, 2181, 3534
PkrI GCNGC 8 cut(s) 64, 791, 2567, 2570, 2977, 3035, 3342, 3471
Ppu21I YACGTR 1 cut(s) 3190
PspEI GGTNACC 1 cut(s) 2355
PspFI CCCAGC 2 cut(s) 2317, 2857
PspN4I GGNNCC 5 cut(s) 1576, 2283, 2850, 2959, 3573
PspOMI GGGCCC 1 cut(s) 2281
PspPI GGNCC 4 cut(s) 1202, 1427, 2281, 2282
PstNI CAGNNNCTG 4 cut(s) 568, 1847, 2571, 3427
PsuI RGATCY 4 cut(s) 730, 2185, 2222, 2699
PvuII CAGCTG 6 cut(s) 62, 992, 1120, 1730, 2305, 2571
RigI GGCCGGCC 1 cut(s) 1824
RseI CAYNNNNRTG 3 cut(s) 1607, 1994, 3156
SaqAI TTAA 5 cut(s) 1677, 2132, 2165, 2946, 3501
SatI GCNGC 8 cut(s) 63, 790, 2566, 2569, 2976, 3034, 3341, 3470
Sau3AI GATC 8 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222, 2699
Sau96I GGNCC 4 cut(s) 1202, 1427, 2281, 2282
ScaI AGTACT 2 cut(s) 1141, 3203
SduI GDGCHC 6 cut(s) 1085, 1248, 2285, 2960, 3167, 3195
SexAI ACCWGGT 1 cut(s) 2844
SfaNI GCATC 6 cut(s) 722, 1530, 2330, 2454, 2613, 3209
SfcI CTRYAG 2 cut(s) 3241, 3288
SinI GGWCC 2 cut(s) 1202, 1427
SmiMI CAYNNNNRTG 3 cut(s) 1607, 1994, 3156
SmlI CTYRAG 9 cut(s) 132, 425, 719, 736, 935, 1019, 1391, 1420, 1676
SmoI CTYRAG 9 cut(s) 132, 425, 719, 736, 935, 1019, 1391, 1420, 1676
SpeI ACTAGT 2 cut(s) 887, 1909
SseBI AGGCCT 2 cut(s) 192, 3286
SsiI CCGC 4 cut(s) 1901, 2076, 2512, 3469
SspI AATATT 1 cut(s) 2143
StuI AGGCCT 2 cut(s) 192, 3286
TaiI ACGT 4 cut(s) 216, 2046, 2613, 3192
TaqI TCGA 6 cut(s) 225, 1248, 2494, 3144, 3217, 3375
TaqII GACCGA 2 cut(s) 405, 2808
TatI WGTACW 4 cut(s) 300, 1139, 2901, 3201
TauI GCSGC 1 cut(s) 3472
Tru1I TTAA 5 cut(s) 1677, 2132, 2165, 2946, 3501
Tru9I TTAA 5 cut(s) 1677, 2132, 2165, 2946, 3501
TseFI GTSAC 3 cut(s) 1620, 2249, 2776
TseI GCWGC 7 cut(s) 62, 789, 2565, 2568, 2975, 3033, 3340
Tsp45I GTSAC 3 cut(s) 1620, 2249, 2776
TspDTI ATGAA 9 cut(s) 171, 306, 534, 648, 1952, 2921, 2924, 3077, 3102
Van91I CCANNNNNTGG 1 cut(s) 2372
Vha464I CTTAAG 1 cut(s) 1676
VpaK11BI GGWCC 2 cut(s) 1202, 1427
XagI CCTNNNNNAGG 1 cut(s) 2295
XapI RAATTY 5 cut(s) 49, 984, 1069, 1129, 1983
XbaI TCTAGA 1 cut(s) 524
XmiI GTMKAC 2 cut(s) 2721, 3240
XmnI GAANNNNTTC 2 cut(s) 1843, 2547
ZrmI AGTACT 2 cut(s) 1141, 3203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.